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9uo1

Cryo-EM structure of human organic solute transporter Ost-alpha/beta bound with DHEAS

Method: ELECTRON MICROSCOPY D_max: 90.8 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uo1
deposition_date	2025-04-24
title	Cryo-EM structure of human organic solute transporter Ost-alpha/beta bound with DHEAS
keywords	substrate, transport, heterodimer, TRANSPORT PROTEIN; TRANSPORT PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	29.73 Å
rg_electron	28.39 Å
i0	95359900.00
molecular_weight	102980.0 kDa
excluded_volume	140320 Å³
envelope_volume	159190 Å³
shell_volume	44620 Å³
envelope_diameter	95.7 Å
shell_rg	37.38 Å
envelope_rg	28.51 Å
shape_rg	28.38 Å
total_rg	29.53 Å
total_atoms	7236
n_residues	748
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	90.8 Å
rg_real	29.59 Å
rg_real_error	0.57 Å
i0_real	9.5360e+07
i0_real_error	1.4610e+06
rg_reciprocal	29.65 Å
i0_reciprocal	95360000.0000
total_estimate	0.9097
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	39.4 Å
skewness	0.138
kurtosis	-0.501
angular_range	— – 0.2650 Å⁻¹
current_alpha	0.0000
highest_alpha	18510000.0000
n_real_points	54
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.956; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.994; Smooth: 0.958

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4. Crystallography & Experiment

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5. Entities & Polymer Info (9)

Entity 1 polymer

description	Organic solute transporter subunit alpha
formula_weight	38967.1 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,C
atom_count	7238

MEPGRTQIKLDPRYTADLLEVLKTNYGIPSACFSQPPTAAQLLRALGPVELALTSILTLLALGSIAIFLEDAVYLYKNTLCPIKRRTLLWKSSAPTVVSVLCCFGLWIPRSLVLVEMTITSFYAVCFYLLMLVMVEGFGGKEAVLRTLRDTPMMVHTGPCCCCCPCCPRLLLTRKKLQLLMLGPFQYAFLKITLTLVGLFLVPDGIYDPADISEGSTALWINTFLGVSTLLALWTLGIISRQARLHLGEQNMGAKFALFQVLLILTALQPSIFSVLANGGQIACSPPYSSKTRSQVMNCHLLILETFLMTVLTRMYYRRKDHKVGYETFSSPDLDLNLKAGGSDYKDDDDK

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 2 polymer

description	Organic solute transporter subunit beta
formula_weight	15943.9 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	B,D

MEHSEGAPGDPAGTVVPQELLEEMLWFFRVEDASPWNHSILALAAVVVIISMVLLGRSIQASRKEKMQPPEKETPEVLHLDEAKDHNSLNNLRETLLSEKPNLAQVELELKERDVLSVFLPDVPETESGGSHHHHHHHHHH

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 3 non-polymer

description	(7E,21R,24S)-27-amino-24-hydroxy-18,24-dioxo-19,23,25-trioxa-24lambda~5~-phosphaheptacos-7-en-21-yl (9Z,12E)-octadeca-9,12-dienoate
formula_weight	742.0 kDa
src_method	nat
entity_count	4

Entity 4 non-polymer

description	PHOSPHATIDYL ETHANOL
formula_weight	717.0 kDa
src_method	syn
entity_count	2

Entity 5 non-polymer

description	1-O-OCTADECYL-SN-GLYCERO-3-PHOSPHOCHOLINE
formula_weight	510.7 kDa
src_method	nat
entity_count	2

Entity 6 non-polymer

description	CHOLESTEROL
formula_weight	386.7 kDa
src_method	syn
entity_count	32

Entity 7 non-polymer

description	PALMITIC ACID
formula_weight	256.4 kDa
src_method	nat
entity_count	12

Entity 8 non-polymer

description	17-oxoandrost-5-en-3beta-yl hydrogen sulfate
formula_weight	368.5 kDa
src_method	nat
entity_count	4

Entity 9 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	2

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6. Citations (1)

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resolution	2.90 Å
resolution_method	—
num_particles	81957

fit	/app/data/saxs_computation/uo/9uo1/9uo1.fit
pddf	/app/data/pr_computation/uo/9uo1/9uo1.pr.out
profile	/app/data/saxs_computation/uo/9uo1/9uo1.dat

Cryo-EM structure of human organic solute transporter Ost-alpha/beta bound with DHEAS

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

EM 3D

5. Entities & Polymer Info (9)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 non-polymer

Entity 6 non-polymer

Entity 7 non-polymer

Entity 8 non-polymer

Entity 9 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)