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9uuk

Crystal structure of the mu2 subunit of the clathrin-adaptor protein 2 (AP2) bound to HPV16 E7(residues 22-32; S31E and S32E)

Method: X-RAY DIFFRACTION D_max: 85.3 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uuk
deposition_date	2025-05-07
last_revision	2025-09-10
title	Crystal structure of the mu2 subunit of the clathrin-adaptor protein 2 (AP2) bound to HPV16 E7(residues 22-32; S31E and S32E)
keywords	mu2, clathrin-adaptor protein 2, AP2, HPV16, E7, CR2, ENDOCYTOSIS; ENDOCYTOSIS
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	23.70 Å
rg_electron	23.13 Å
i0	12545900.00
molecular_weight	27654.0 kDa
excluded_volume	35144 Å³
envelope_volume	43218 Å³
shell_volume	17206 Å³
envelope_diameter	87.1 Å
shell_rg	27.90 Å
envelope_rg	23.48 Å
shape_rg	23.13 Å
total_rg	23.83 Å
total_atoms	1940
n_residues	245
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	85.3 Å
rg_real	24.02 Å
rg_real_error	0.88 Å
i0_real	1.2550e+07
i0_real_error	1.7310e+05
rg_reciprocal	23.95 Å
i0_reciprocal	12550000.0000
total_estimate	0.7747
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	20.0 Å
skewness	0.612
kurtosis	-0.205
angular_range	— – 0.3350 Å⁻¹
current_alpha	0.0000
highest_alpha	5105000.0000
n_real_points	65
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.554; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.417; Smooth: 0.991

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	AP-2 complex subunit mu
formula_weight	32124.7 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	1940

GHMQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli

Entity 2 polymer

description	Protein E7
formula_weight	1418.5 kDa
mutation	S31E, S32E
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

LYCYEQLNDEE

Source

organism	Human papillomavirus 16
ncbi_taxonomy_id	333760
host_organism	synthetic construct

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6. Citations (1)

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length_a	125.206 Å
length_b	125.206 Å
length_c	73.492 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 64
int_tables_number	172
crystal_system	—

resolution	3.199 Å
resolution_low	35.794 Å
r_factor	0.2092
r_work	0.2054
r_free	0.2438

fit	/app/data/saxs_computation/uu/9uuk/9uuk.fit
pddf	/app/data/pr_computation/uu/9uuk/9uuk.pr.out
profile	/app/data/saxs_computation/uu/9uuk/9uuk.dat

Crystal structure of the mu2 subunit of the clathrin-adaptor protein 2 (AP2) bound to HPV16 E7(residues 22-32; S31E and S32E)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)