← Back to search

9uvv

Cryo-EM structure of Vasopressin receptor 2 (V2R)-BRIL/anti BRIL SRP2070 Fab antibody complex with tolvaptan

Method: ELECTRON MICROSCOPY D_max: 112.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	9uvv
deposition_date	2025-05-11
title	Cryo-EM structure of Vasopressin receptor 2 (V2R)-BRIL/anti BRIL SRP2070 Fab antibody complex with tolvaptan
keywords	GPCR, V2R, vasopressin, tolvaptan, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	34.95 Å
rg_electron	34.74 Å
i0	223654000.00
molecular_weight	80785.0 kDa
excluded_volume	78453 Å³
envelope_volume	147690 Å³
shell_volume	36830 Å³
envelope_diameter	119.5 Å
shell_rg	39.74 Å
envelope_rg	34.09 Å
shape_rg	34.74 Å
total_rg	35.03 Å
total_atoms	6121
n_residues	785
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	112.8 Å
rg_real	34.97 Å
rg_real_error	0.95 Å
i0_real	2.2370e+08
i0_real_error	3.5310e+06
rg_reciprocal	34.96 Å
i0_reciprocal	223700000.0000
total_estimate	0.8993
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	35.1 Å
skewness	0.235
kurtosis	-0.704
angular_range	— – 0.2250 Å⁻¹
current_alpha	0.0000
highest_alpha	11900000.0000
n_real_points	46
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.933; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.956; Smooth: 0.930

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (4)

Entity 1 polymer

description	Vasopressin V2 receptor,Soluble cytochrome b562
formula_weight	53595.0 kDa
mutation	M243W/H338I/R342L
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	6121

MKTIIALSYIFCLVFADYKDDDDAENLYFQGMLMASTTSAVPGHPSLPSLPSNSSQERPLDTRDPLLARAELALLSIVFVAVALSNGLVLAALARRGRRGHWAPIHVFIGHLCLADLAVALFQVLPQLAWKATDRFRGPDALCRAVKYLQMVGMYASSYMILAMTLDRHRAICRPMLAYRHGSGAHWNRPVLVAWAFSLLLSLPQLFIFAQRNVEGGSGVTDCWACFAEPWGRRTYVTWIALMVFVAPTLGIAACQVLIFREIHASLADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLAVAKTVRMTLVIVVVYVLCWAPFFLVQLWAAWDPEAPLEGAPFVLLMLLASLNSCTNPWIYASFSSSVSSELRSLLCCARGRTPPSLGPQDESCTTASSSLAKDTSSLEVLFQ

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 2 polymer

description	Light chain of anti-BRIL SRP2070 fab antibody
formula_weight	26065.0 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	L

MEAPAQLLFLLLLWLPSSRAMDIVLTQSPATLSVTPGDRVSLSCRASQSVSNYLHWYQQKSHESPRLLIKYASQSISGIPSRFSGSGSGTDFTLSINSVETEDFGMYFCQQSNSWPLTFGAGTKLELRRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC

Source

organism	Mus musculus
ncbi_taxonomy_id	10090
host_organism	Cricetulus griseus

Entity 3 polymer

description	Heavy chain of anti-BRIL SRP2070 fab antibody
formula_weight	31421.2 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	H

MEAPAQLLFLLLLWLPSSRAMQIQLQQSGPELVKPGASVKISCKASGYTFTDFYINWMKQRPGQGLEWIGWIFPGSGNTHYNEKFKGKATLIVDTSSSTAFMQLNSLTSEDSAVYFCTRPVSYYYDFDYWGQGTTLTVSSAKTTAPSVYPLAPVCGDTSGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGCLEVLFQGPGHHHHHHHHSAWSHPQFEKGGGSGGGGSGGSAWSHPQFEK

Source

organism	Mus musculus
ncbi_taxonomy_id	10090
host_organism	Cricetulus griseus

Entity 4 non-polymer

description	(R)-Tolvaptan
formula_weight	448.9 kDa
src_method	syn
entity_count	1

▶

6. Citations (1)

▶

length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.020 Å
resolution_low	3.020 Å
r_factor	—
r_work	0.3579
r_free	—

resolution	3.02 Å
resolution_method	—
num_particles	380888

fit	/app/data/saxs_computation/uv/9uvv/9uvv.fit
pddf	/app/data/pr_computation/uv/9uvv/9uvv.pr.out
profile	/app/data/saxs_computation/uv/9uvv/9uvv.dat

Cryo-EM structure of Vasopressin receptor 2 (V2R)-BRIL/anti BRIL SRP2070 Fab antibody complex with tolvaptan

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)