Cryo-EM structure of Vasopressin receptor 2 (V2R)-BRIL/anti BRIL SRP2070 Fab antibody complex with tolvaptan, focused on receptor

Method: ELECTRON MICROSCOPY D_max: 70.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uvw
deposition_date	2025-05-11
title	Cryo-EM structure of Vasopressin receptor 2 (V2R)-BRIL/anti BRIL SRP2070 Fab antibody complex with tolvaptan, focused on receptor
keywords	GPCR, V2R, vasopressin, tolvaptan, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	20.96 Å
rg_electron	19.91 Å
i0	27334900.00
molecular_weight	27806.0 kDa
excluded_volume	27453 Å³
envelope_volume	45120 Å³
shell_volume	19272 Å³
envelope_diameter	72.1 Å
shell_rg	26.12 Å
envelope_rg	20.47 Å
shape_rg	19.88 Å
total_rg	20.64 Å
total_atoms	2113
n_residues	266
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	70.5 Å
rg_real	20.96 Å
rg_real_error	0.48 Å
i0_real	2.7330e+07
i0_real_error	3.5980e+05
rg_reciprocal	20.96 Å
i0_reciprocal	27330000.0000
total_estimate	0.8790
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	24.5 Å
skewness	0.346
kurtosis	-0.349
angular_range	— – 0.3800 Å⁻¹
current_alpha	0.0000
highest_alpha	2900000.0000
n_real_points	70
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.834; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.979; Smooth: 0.941

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.890 Å
resolution_low	2.890 Å
r_factor	—
r_work	0.4036
r_free	—

resolution	2.89 Å
resolution_method	—
num_particles	195895

description	Vasopressin V2 receptor,Soluble cytochrome b562
formula_weight	53595.0 kDa
mutation	M243W/H338I/R342L
src_method	man
entity_count	1

Cryo-EM structure of Vasopressin receptor 2 (V2R)-BRIL/anti BRIL SRP2070 Fab antibody complex with tolvaptan, focused on receptor

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

description	(R)-Tolvaptan
formula_weight	448.9 kDa
src_method	syn
entity_count	1

fit	/app/data/saxs_computation/uv/9uvw/9uvw.fit
pddf	/app/data/pr_computation/uv/9uvw/9uvw.pr.out
profile	/app/data/saxs_computation/uv/9uvw/9uvw.dat

type	polypeptide(L)
strand_id	A
atom_count	2113