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9w02

Cryo-EM structure of TasH-tigRNA-ssDNA complex

Method: ELECTRON MICROSCOPY D_max: 92.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9w02
deposition_date	2025-07-23
title	Cryo-EM structure of TasH-tigRNA-ssDNA complex
keywords	protein complex, ANTIVIRAL PROTEIN; ANTIVIRAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	29.03 Å
rg_electron	29.23 Å
i0	111450000.00
molecular_weight	70717.0 kDa
excluded_volume	83103 Å³
envelope_volume	109260 Å³
shell_volume	32237 Å³
envelope_diameter	96.9 Å
shell_rg	35.59 Å
envelope_rg	28.73 Å
shape_rg	29.29 Å
total_rg	29.59 Å
total_atoms	4900
n_residues	532
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	92.8 Å
rg_real	29.10 Å
rg_real_error	0.69 Å
i0_real	1.1140e+08
i0_real_error	1.5690e+06
rg_reciprocal	29.07 Å
i0_reciprocal	111400000.0000
total_estimate	0.8837
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	30.4 Å
skewness	0.413
kurtosis	-0.414
angular_range	— – 0.2750 Å⁻¹
current_alpha	0.0000
highest_alpha	14450000.0000
n_real_points	56
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.898; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.959; Smooth: 0.833

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	HNH nuclease domain-containing protein
formula_weight	40031.2 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	4900

MNKQVLKEQASHCEITGAPLAGLPELVDVDRITERFQGGTYTPDNTRVLTPRAHMERHGILRERDQWLEELKAMMDDRAQTMKVVMKMNNQLLAYQRQTDHARQSTEQFLQDTLDASNKRLAQIDREVTKHIKHAKDPLAQAAMGVPGVGPITVAGLQTYVDLEKAKSASALWAYIGIDKPSHDRYTKGEAGGGNKTLRTMVWNMANSMIKNRKCPYRTVYEQTKERLAVSEKVTKSRNTQGQLIECAWKDTKPSHRHGAALRAVMKHFLADYWFVGRELAGLDTRPLYVQEKLGHTGIVQPQERGWEWGGSWSHPQFEKGGGSGGGSGGSAWSHPQFEKNLYFQSGSHHHHHH

Source

organism	Salicola phage CGphi29
ncbi_taxonomy_id	754067
host_organism	Escherichia coli

Entity 2 polymer

description	RNA (36-MER)
formula_weight	11948.2 kDa
src_method	man
entity_count	1

Polymer

type	polyribonucleotide
strand_id	C

AGUCAUUCCGUUAAAGACAACCACGGAGACGAAGCGA

Source

organism	Salicola phage CGphi29
ncbi_taxonomy_id	754067
host_organism	Escherichia coli

Entity 3 polymer

description	DNA (38-MER)
formula_weight	11784.6 kDa
src_method	man
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	D

GTCCTGTAACACGGAGACGTAACGGAATTGCCTTAGGG

Source

organism	Salicola phage CGphi29
ncbi_taxonomy_id	754067
host_organism	Escherichia coli

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.72 Å
resolution_method	—
num_particles	219563

fit	/app/data/saxs_computation/w0/9w02/9w02.fit
pddf	/app/data/pr_computation/w0/9w02/9w02.pr.out
profile	/app/data/saxs_computation/w0/9w02/9w02.dat

Cryo-EM structure of TasH-tigRNA-ssDNA complex

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)