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9w03

Cryo-EM structure of TasH-tigRNA complex

Method: ELECTRON MICROSCOPY D_max: 97.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9w03
deposition_date	2025-07-23
title	Cryo-EM structure of TasH-tigRNA complex
keywords	protein complex, ANTIVIRAL PROTEIN; ANTIVIRAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	29.96 Å
rg_electron	30.18 Å
i0	93272700.00
molecular_weight	66166.0 kDa
excluded_volume	78561 Å³
envelope_volume	105660 Å³
shell_volume	30554 Å³
envelope_diameter	100.7 Å
shell_rg	35.84 Å
envelope_rg	29.73 Å
shape_rg	30.23 Å
total_rg	30.49 Å
total_atoms	4596
n_residues	513
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	97.2 Å
rg_real	30.08 Å
rg_real_error	0.53 Å
i0_real	9.3270e+07
i0_real_error	1.1860e+06
rg_reciprocal	30.03 Å
i0_reciprocal	93270000.0000
total_estimate	0.8803
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	29.4 Å
skewness	0.408
kurtosis	-0.531
angular_range	— – 0.2650 Å⁻¹
current_alpha	0.0001
highest_alpha	16350000.0000
n_real_points	54
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.877; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.920; Smooth: 0.888

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	HNH nuclease domain-containing protein
formula_weight	40031.2 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	4596

MNKQVLKEQASHCEITGAPLAGLPELVDVDRITERFQGGTYTPDNTRVLTPRAHMERHGILRERDQWLEELKAMMDDRAQTMKVVMKMNNQLLAYQRQTDHARQSTEQFLQDTLDASNKRLAQIDREVTKHIKHAKDPLAQAAMGVPGVGPITVAGLQTYVDLEKAKSASALWAYIGIDKPSHDRYTKGEAGGGNKTLRTMVWNMANSMIKNRKCPYRTVYEQTKERLAVSEKVTKSRNTQGQLIECAWKDTKPSHRHGAALRAVMKHFLADYWFVGRELAGLDTRPLYVQEKLGHTGIVQPQERGWEWGGSWSHPQFEKGGGSGGGSGGSAWSHPQFEKNLYFQSGSHHHHHH

Source

organism	Salicola phage CGphi29
ncbi_taxonomy_id	754067
host_organism	Escherichia coli

Entity 2 polymer

description	RNA (36-MER)
formula_weight	11948.2 kDa
src_method	man
entity_count	1

Polymer

type	polyribonucleotide
strand_id	C

AGUCAUUCCGUUAAAGACAACCACGGAGACGAAGCGA

Source

organism	Salicola phage CGphi29
ncbi_taxonomy_id	754067
host_organism	Escherichia coli

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.30 Å
resolution_method	—
num_particles	107787

fit	/app/data/saxs_computation/w0/9w03/9w03.fit
pddf	/app/data/pr_computation/w0/9w03/9w03.pr.out
profile	/app/data/saxs_computation/w0/9w03/9w03.dat

Cryo-EM structure of TasH-tigRNA complex

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)