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9wag

Yeast-expressed polio type 1 expanded virus-like particles

Method: ELECTRON MICROSCOPY D_max: 99.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9wag
deposition_date	2025-08-12
title	Yeast-expressed polio type 1 expanded virus-like particles
keywords	Poliovirus type 1, virus-like particles, expanded state, VIRAL PROTEIN, VIRUS LIKE PARTICLE; VIRUS LIKE PARTICLE
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	29.98 Å
rg_electron	29.19 Å
i0	77687300.00
molecular_weight	70013.0 kDa
excluded_volume	87875 Å³
envelope_volume	116010 Å³
shell_volume	33983 Å³
envelope_diameter	106.0 Å
shell_rg	35.47 Å
envelope_rg	29.49 Å
shape_rg	29.18 Å
total_rg	29.85 Å
total_atoms	4930
n_residues	625
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	99.6 Å
rg_real	30.05 Å
rg_real_error	0.51 Å
i0_real	7.7690e+07
i0_real_error	1.0450e+06
rg_reciprocal	30.02 Å
i0_reciprocal	77690000.0000
total_estimate	0.8835
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	31.9 Å
skewness	0.417
kurtosis	-0.299
angular_range	— – 0.2650 Å⁻¹
current_alpha	0.0002
highest_alpha	21920000.0000
n_real_points	54
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.869; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.972; Smooth: 0.901

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	Capsid protein VP1
formula_weight	33478.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	4930

GLGQMLESMIDNTVRETVGAATSRDALPNTEASGPTHSKEIPALTAVETGATNPLVPSDTVQTRHVVQHRSRSESSIESFFARGACVTIMTVDNSASTTNKDKLFAVWKITYKDTVQLRRKLEFFTYSRFDMELTFVVTANFTETNNGHALNQVYQIMYVPPGAPVPEKWDDYTWQTSSNPSIFYTYGTAPARISVPYVGISNAYSHFYDGFSKVPLKDQSAALGDSLYGAASLNDFGILAVRVVNDHNPTKVTSKIRVYLKPKHIRVWCPRPPRAVAYYGPGVDYKDGTLTPLSTKDLTTY

Source

organism	Poliovirus 1
ncbi_taxonomy_id	12080
host_organism	Komagataella pastoris

Entity 2 polymer

description	Capsid protein VP2
formula_weight	30075.8 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

SPNIEACGYSDRVLQLTLGNSTITTQEAANSVVAYGRWPEYLRDSEANPVDQPTEPDVAACRFYTLDTVSWTKESRGWWWKLPDALRDMGLFGQNMYYHYLGRSGYTVHVQCNASKFHQGALGVFAVPEMCLAGDSNTTTMHTSYQNANPGEKGGTFTGTFTPDNNQTSPARRFCPVDYLLGNGTLLGNAFVFPHQIINLRTNNCATLVLPYVNSLSIDSMVKHNNWGIAILPLAPLNFASESSPEIPITLTIAPMCCEFNGLRNITLPRLQ

Source

organism	Poliovirus 1
ncbi_taxonomy_id	12080
host_organism	Komagataella pastoris

Entity 3 polymer

description	Capsid protein VP3
formula_weight	26547.5 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C

GLPVMNTPGSNQYLTADNFQSPCALPEFDVTPPIDIPGEVKNMMELAEIDTMIPFDLSATKKNTMEMYRVRLSDKPHTDDPILCLSLSPASDPRLSHTMLGEILNYYTHWAGSLKFTFLFCGSMMATGKLLVSYAPPGADPPKKRKEAMLGTHVIWDIGLQSSCTMVVPWISNTTYRQTIDDSFTEGGYISVFYQTRIVVPLSTPREMDILGFVSACNDFSVRLLRDTTHIEQKALAQ

Source

organism	Poliovirus 1
ncbi_taxonomy_id	12080
host_organism	Komagataella pastoris

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.950 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.95 Å
resolution_method	—
num_particles	211442

fit	/app/data/saxs_computation/wa/9wag/9wag.fit
pddf	/app/data/pr_computation/wa/9wag/9wag.pr.out
profile	/app/data/saxs_computation/wa/9wag/9wag.dat

Yeast-expressed polio type 1 expanded virus-like particles

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)