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9wzs

Cryo-EM structure of Fks2 in complex with enfumafungin

Method: ELECTRON MICROSCOPY Dmax: 125.0 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

1,3-beta-glucan synthase component GSC2

Saccharomyces cerevisiae S288C

UniProt P40989

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Monomer Protein × 1 其他Polymer 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 1–1895 Not recorded 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ERG ERGOSTEROL × 10 A1E06 Enfumafungin × 1 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.32 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9WZS Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9LU8 Structure of yeast 1,3-beta-glucan synthase FKS2 Deposited 2025-02-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–1895（1895 aa）
Not recorded MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.99 Å
9WZT Cryo-EM structure of Fks2 in apo state Deposited 2025-09-29 Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–1895（1895 aa）
Not recorded AV0 Lauryl Maltose Neopentyl Glycol × 2 ERG ERGOSTEROL × 9 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.64 Å
9X04 Cryo-EM structure of Fks2 with intact active site Deposited 2025-09-29 Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–1895（1895 aa）
Not recorded ERG ERGOSTEROL × 20 A1E06 Enfumafungin × 1 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.41 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name FKS2_YEAST
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–1895； UniProt 1–1895

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 9wzs

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 9wzs
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2. Structure Basics 2. Structure Basics

Entry ID entry_id9wzs
Deposition date deposition_date2025-09-29
Structure title titleCryo-EM structure of Fks2 in complex with enfumafungin
Keywords keywordsComplex, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier38.80 Å
Radius of gyration Rg (electron density) rg_electron38.21 Å
Forward intensity I(0) i0883180000.00
Molecular weight molecular_weight170540.0 kDa
Excluded volume excluded_volume170170 ų
Envelope volume envelope_volume312820 ų
Hydration-shell volume shell_volume66133 ų
Envelope diameter envelope_diameter137.5 Å
Shell Rg shell_rg45.78 Å
Envelope Rg envelope_rg38.04 Å
Shape Rg shape_rg38.18 Å
Total Rg total_rg38.60 Å
Total atoms total_atoms13280
Residues n_residues1542
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax125.0 Å
Rg (real space) rg_real38.68 Å
Rg uncertainty (real space) rg_real_error0.75 Å
I(0) (real space) i0_real8.8320e+08
I(0) uncertainty (real space) i0_real_error1.3890e+07
Rg (reciprocal space) rg_reciprocal38.76 Å
I(0) (reciprocal space) i0_reciprocal883200000.0000
Solution quality estimate total_estimate0.8924
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary45.7 Å
Skewness Skewness skewness0.260
Kurtosis Kurtosis kurtosis-0.435
Angular range angular_range— – 0.2050 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha105300000.0000
Real-space data points n_real_points42
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.909; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.876
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (5)

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8. Citations (1)

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9. Files and Curves (10)