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9yee

Rod-hook protein in Vibrio cholerae at assembled, opened state

Method: ELECTRON MICROSCOPY D_max: 221.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9yee
deposition_date	2025-09-24
title	Rod-hook protein in Vibrio cholerae at assembled, opened state
keywords	In situ cryo-EM, sheathed flagellar motor, Vibrio cholerae, rod-hook, assembled state, MOTOR PROTEIN; MOTOR PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	74.52 Å
rg_electron	74.82 Å
i0	27982200000.00
molecular_weight	1375000.0 kDa
excluded_volume	1700600 Å³
envelope_volume	2327700 Å³
shell_volume	244830 Å³
envelope_diameter	297.3 Å
shell_rg	81.67 Å
envelope_rg	76.60 Å
shape_rg	74.85 Å
total_rg	74.78 Å
total_atoms	96589
n_residues	12773
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	221.8 Å
rg_real	73.30 Å
rg_real_error	0.81 Å
i0_real	2.7610e+10
i0_real_error	5.1620e+08
rg_reciprocal	73.80 Å
i0_reciprocal	27930000000.0000
total_estimate	0.8344
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	84.5 Å
skewness	0.474
kurtosis	-0.196
angular_range	— – 0.1050 Å⁻¹
current_alpha	0.1840
highest_alpha	4384000000.0000
n_real_points	22
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.901; Stabil: 0.998; Sysdev: 1.000; Positv: 1.000; Valcen: 0.970; Smooth: 0.093

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	Flagellar basal-body rod protein FlgG
formula_weight	28114.3 kDa
src_method	nat
entity_count	27

Polymer

type	polypeptide(L)
strand_id	Ba,Bb,Bc,Bd,Be,Bf,Bg,Bh,Bi,Bj,Bk,Bl,Bm,Bn,Bo,Bp,Bq,Br,Bs,Bt,Bu,Bv,Bw,Bx,By,Bz,Cb
atom_count	96589

MQPALWVSKTGLDAQQTNIATISNNLANASTIGFKKGRAVFEDLFYQNINQPGAQSSQNTRLPSGLMLGAGSKVVATQKVHTNGNVQTTSNSLDMMIEGDGFFQILMPDGNIAYSRNGQFTLDDEGAIVTSGAGYRLQPEIIIPEDAISITIGNDGEVSVRVRGQQDNQVLGQITTVDFINPGGLEPVGQNLYLPTGASGEPQEGVPGLDGLGDIRQSMLETSNVNVTEELVNMIEAQRVYEMNSKVISAVDKMMSFVNQQL

Entity 2 polymer

description	Flagellar basal-body rod protein FlgF
formula_weight	26778.3 kDa
src_method	nat
entity_count	5

Polymer

type	polypeptide(L)
strand_id	Ca,Cc,Cd,Cv,Cw

MDRALFLAMSGAKQNMQAMQLRANNLANVSTTGFRADLAQARSMQAYGEGLPSRVFSMTERPGHNFAQGSVITTGRDLDITIQGDGWISVLDKTGKEGLTRNGNLRIDETGLLLNGNGHPVLGETGAPITLPVPLNKVEIGGDGTISVRPQGAPADAMEVVDRIKLVRLNDQSLFKDVNGLFRATDPNTQFEADASVKILTGALEGSNVNAIGEMTSLIDLQRQFEMQVKMMSTAEEMDKASDSLLRSS

Entity 3 polymer

description	Flagellar hook protein FlgE
formula_weight	46880.3 kDa
src_method	nat
entity_count	17

Polymer

type	polypeptide(L)
strand_id	Ce,Cf,Cg,Ch,Ci,Cj,Ck,Cl,Cm,Cn,Co,Cp,Cq,Cr,Cs,Ct,Cu

MSYVSLSGLSAAQMDLNTTSNNIANANTFGFKESRAEFGDVYSTSLFTNAKTTPGQGVQAAKVAQQFHEGSSIYTNNPLDLRIAGTGFFAVAKDRLVPQQNELTRNGAFHLDKNSFMVTANDEFLLGYEVNPDTGDVLSYEPKPINIPPQFGKPKQTANIDLGANLPANGDLKDPALFDITDPETYNRTTSSTIYDSMGQPYKLTTYYLKDMNQANTWQTYYTVTDKTGEKPINVVGGDAASPTGHVGHTMRFNNDGTLSSLNNGQPIVTEPLGGGANPVDLNGADVNQTLSFSLDSATQFAAPFELTKFDQDGATTGFLTKIDFDENGSVLATYSNGINTTLGRVALVRVANEQGLDKKGGTQWDATQFSGAKIWGESNKGSFGSISNGSLEQSNIDMTQELVDLISAQRNFQANSRALEVHNGLQQNILQIR

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	3.44 Å
resolution_method	—
num_particles	77446

fit	/app/data/saxs_computation/ye/9yee/9yee.fit
pddf	/app/data/pr_computation/ye/9yee/9yee.pr.out
profile	/app/data/saxs_computation/ye/9yee/9yee.dat

Rod-hook protein in Vibrio cholerae at assembled, opened state

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)