Stable open sheep connexin-50 in amphipol at low pH

Method: ELECTRON MICROSCOPY D_max: 152.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9z7w
deposition_date	2025-11-17
title	Stable open sheep connexin-50 in amphipol at low pH
keywords	connexin, gap junction, cryo-EM, pH regulation, lipid gating, large-pore channel, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	45.09 Å
rg_electron	45.74 Å
i0	772455000.00
molecular_weight	250790.0 kDa
excluded_volume	321450 Å³
envelope_volume	415300 Å³
shell_volume	76176 Å³
envelope_diameter	149.2 Å
shell_rg	49.66 Å
envelope_rg	45.89 Å
shape_rg	45.76 Å
total_rg	45.85 Å
total_atoms	35388
n_residues	2184
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	152.8 Å
rg_real	45.32 Å
rg_real_error	1.48 Å
i0_real	7.7250e+08
i0_real_error	1.3340e+07
rg_reciprocal	45.10 Å
i0_reciprocal	772200000.0000
total_estimate	0.8302
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	48.0 Å
skewness	0.510
kurtosis	-0.335
angular_range	— – 0.1750 Å⁻¹
current_alpha	0.0000
highest_alpha	203500000.0000
n_real_points	36
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.692; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.969; Smooth: 0.744

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4. Crystallography & Experiment

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5. Entities & Polymer Info (1)

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.700 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.70 Å
resolution_method	—
num_particles	127102

description	Gap junction alpha-8 protein
formula_weight	49216.8 kDa
src_method	man
entity_count	12

Stable open sheep connexin-50 in amphipol at low pH

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (1)

Entity 1 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

type	polypeptide(L)
strand_id	A,B,C,D,E,F,G,H,I,J,K,L
atom_count	35388

organism	Ovis aries
common_name	sheep
ncbi_taxonomy_id	9940
host_organism	Escherichia coli

fit	/app/data/saxs_computation/z7/9z7w/9z7w.fit
pddf	/app/data/pr_computation/z7/9z7w/9z7w.pr.out
profile	/app/data/saxs_computation/z7/9z7w/9z7w.dat