Asymmetrically gated state sheep connexin-50 in DMPC nanodiscs at low pH

Method: ELECTRON MICROSCOPY D_max: 158.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9za4
deposition_date	2025-11-19
title	Asymmetrically gated state sheep connexin-50 in DMPC nanodiscs at low pH
keywords	connexin, gap junction, cryo-EM, pH regulation, lipid gating, large-pore channel, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	45.49 Å
rg_electron	46.25 Å
i0	671962000.00
molecular_weight	287540.0 kDa
excluded_volume	389800 Å³
envelope_volume	502450 Å³
shell_volume	88051 Å³
envelope_diameter	150.2 Å
shell_rg	53.03 Å
envelope_rg	46.10 Å
shape_rg	46.25 Å
total_rg	46.55 Å
total_atoms	42816
n_residues	2202
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	158.1 Å
rg_real	45.84 Å
rg_real_error	1.68 Å
i0_real	6.7200e+08
i0_real_error	1.1810e+07
rg_reciprocal	45.50 Å
i0_reciprocal	671700000.0000
total_estimate	0.8100
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	45.2 Å
skewness	0.554
kurtosis	-0.333
angular_range	— – 0.1750 Å⁻¹
current_alpha	0.0000
highest_alpha	92070000.0000
n_real_points	36
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.578; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.915; Smooth: 0.878

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.400 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.40 Å
resolution_method	—
num_particles	162334

description	Gap junction alpha-8 protein
formula_weight	49216.8 kDa
src_method	nat
entity_count	12

Asymmetrically gated state sheep connexin-50 in DMPC nanodiscs at low pH

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 non-polymer

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

type	polypeptide(L)
strand_id	A,B,C,D,E,F,G,H,I,J,K,L
atom_count	43524

description	1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE
formula_weight	677.9 kDa
src_method	syn
entity_count	252

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	708

fit	/app/data/saxs_computation/za/9za4/9za4.fit
pddf	/app/data/pr_computation/za/9za4/9za4.pr.out
profile	/app/data/saxs_computation/za/9za4/9za4.dat