Destabilized open state sheep connexin-50 in DMPC nanodiscs at neutral pH

Method: ELECTRON MICROSCOPY D_max: 156.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9z8l
deposition_date	2025-11-18
title	Destabilized open state sheep connexin-50 in DMPC nanodiscs at neutral pH
keywords	connexin, gap junction, cryo-EM, pH regulation, lipid gating, large-pore channel, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	46.24 Å
rg_electron	46.94 Å
i0	698328000.00
molecular_weight	297030.0 kDa
excluded_volume	403970 Å³
envelope_volume	528080 Å³
shell_volume	90581 Å³
envelope_diameter	154.2 Å
shell_rg	53.74 Å
envelope_rg	47.10 Å
shape_rg	46.92 Å
total_rg	47.35 Å
total_atoms	44508
n_residues	2244
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	156.7 Å
rg_real	46.67 Å
rg_real_error	1.46 Å
i0_real	6.9830e+08
i0_real_error	1.2010e+07
rg_reciprocal	46.25 Å
i0_reciprocal	698000000.0000
total_estimate	0.7935
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	46.1 Å
skewness	0.574
kurtosis	-0.333
angular_range	— – 0.1700 Å⁻¹
current_alpha	0.0000
highest_alpha	106600000.0000
n_real_points	35
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.618; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.984; Smooth: 0.473

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.200 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.20 Å
resolution_method	—
num_particles	75136

description	Gap junction alpha-8 protein
formula_weight	49216.8 kDa
src_method	nat
entity_count	12

Destabilized open state sheep connexin-50 in DMPC nanodiscs at neutral pH

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 non-polymer

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

type	polypeptide(L)
strand_id	A,B,C,D,E,F,G,H,I,J,K,L
atom_count	45264

description	1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE
formula_weight	677.9 kDa
src_method	syn
entity_count	276

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	756

fit	/app/data/saxs_computation/z8/9z8l/9z8l.fit
pddf	/app/data/pr_computation/z8/9z8l/9z8l.pr.out
profile	/app/data/saxs_computation/z8/9z8l/9z8l.dat