SAXSdb — 9zq6

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1. Structure Basics

entry_id	9zq6
deposition_date	2025-12-17
title	Structure of SpyCas9 in complex with the anti-CRISPR protein AcrIIA26
keywords	CRISPR, Cas9, Acr, IMMUNE SYSTEM; IMMUNE SYSTEM
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	39.33 Å
rg_electron	38.40 Å
i0	691172000.00
molecular_weight	198510.0 kDa
excluded_volume	241800 Å³
envelope_volume	351190 Å³
shell_volume	73859 Å³
envelope_diameter	126.1 Å
shell_rg	46.70 Å
envelope_rg	36.98 Å
shape_rg	38.37 Å
total_rg	38.95 Å
total_atoms	13893
n_residues	1559
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	122.0 Å
rg_real	39.01 Å
rg_real_error	0.86 Å
i0_real	6.9120e+08
i0_real_error	1.1940e+07
rg_reciprocal	39.21 Å
i0_reciprocal	691300000.0000
total_estimate	0.8984
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	51.8 Å
skewness	0.078
kurtosis	-0.530
angular_range	— – 0.2000 Å⁻¹
current_alpha	0.0000
highest_alpha	83250000.0000
n_real_points	41
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.915; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.973; Smooth: 0.957

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	CRISPR-associated endonuclease Cas9/Csn1
formula_weight	158699.8 kDa
ec_number	3.1.-.-
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	13893

MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD

Source

organism	Streptococcus pyogenes M1 GAS
ncbi_taxonomy_id	160490
host_organism	Escherichia coli B

Entity 2 polymer

description	AcrIIA26
formula_weight	21449.3 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

MKKLYIQTNQFANGELQVENTSYELCDTFKELYSVASNLVDENTLNFVEDNFIEQNYKDEYNGVYENDGDTGEFVGQVFENKVTEEQFKELLEQLEITYTEFDPEEELAKCIANKNRKSEFYGNGLKVIAEYLESISHEDALAVVTYYYFYFGFGYEDQLISDIKDDQEDGVKFEHVERSETI

Source

organism	Streptococcus
ncbi_taxonomy_id	1301
host_organism	Escherichia coli B

Entity 3 polymer

description	sgRNA
formula_weight	38698.0 kDa
src_method	syn
entity_count	1

Polymer

type	polyribonucleotide
strand_id	C

GGAGAGUCUCUCAGCUGGUACAGUUUUAGAGCUAUGCUGUUUUGAAAAAAACAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUCG

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6. Citations (3)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	2.98 Å
resolution_method	—
num_particles	59914

fit	/app/data/saxs_computation/zq/9zq6/9zq6.fit
pddf	/app/data/pr_computation/zq/9zq6/9zq6.pr.out
profile	/app/data/saxs_computation/zq/9zq6/9zq6.dat

Structure of SpyCas9 in complex with the anti-CRISPR protein AcrIIA26

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

6. Citations (3)

7. Files & Curves (10)

Files (7)

Curves (3)