Current Protein Identity：A0A0E2KXL3
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 11BQ Escherichia coli 70S ribosome containing an evolved 16S rRNA (EC-S3.5) Deposited 2026-02-16 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 307 SPD SPERMIDINE × 14 SPM SPERMINE × 1 C CYTIDINE-5'-MONOPHOSPHATE × 1 G GUANOSINE-5'-MONOPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 11DG Chimeric Escherichia coli 70S ribosome containing an evolved Vibrio cholerae 16S rRNA (VC-S4.4) Deposited 2026-02-18 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 272 SPD SPERMIDINE × 14 SPM SPERMINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 11EL Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Vibrio cholerae (VC-ST) Deposited 2026-02-19 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 308 SPD SPERMIDINE × 16 SPM SPERMINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 11FV Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Pseudomonas aeruginosa (PA-S3.3) Deposited 2026-02-21 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain z
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 307 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 11GG Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Pseudomonas aeruginosa (PA-ST) Deposited 2026-02-22 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain z
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 307 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.02 Å |
| 5U9F 3.2 A cryo-EM ArfA-RF2 ribosome rescue complex (Structure II) Deposited 2016-12-16 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 360 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 5U9G 3.2 A cryo-EM ArfA-RF2 ribosome rescue complex (Structure I) Deposited 2016-12-16 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 348 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6OGF 70S termination complex with RF2 bound to the UGA codon. Partially rotated ribosome with RF2 bound (Structure III). Deposited 2019-04-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain W
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6OGG 70S termination complex with RF2 bound to the UGA codon. Rotated ribosome with RF2 bound (Structure IV). Deposited 2019-04-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain W
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6OGI 70S termination complex with RF2 bound to the UAG codon. Rotated ribosome conformation (Structure V) Deposited 2019-04-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain W
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 6OM6 Structure of trans-translation inhibitor bound to E. coli 70S ribosome with P site tRNA Deposited 2019-04-18 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain w
1–75（75 aa）
|
Not recorded | KKL 4-amino-N-[5-(4-ethynylphenyl)-1,3,4-oxadiazol-2-yl]benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Blot time: 3.5 seconds
|
Resolution 3.10 Å |
| 6VYQ Escherichia coli transcription-translation complex A1 (TTC-A1) containing an 15 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6VYR Escherichia coli transcription-translation complex A1 (TTC-A1) containing an 18 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6VYS Escherichia coli transcription-translation complex A1 (TTC-A1) containing a 21 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6VYW Escherichia coli transcription-translation complex C3 (TTC-C3) containing mRNA with a 27 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 6VYX Escherichia coli transcription-translation complex C4 (TTC-C4) containing mRNA with a 21 nt long spacer, transcription factor NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.90 Å |
| 6VYY Escherichia coli transcription-translation complex C5 (TTC-C5) containing mRNA with a 21 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.90 Å |
| 6VYZ Escherichia coli transcription-translation complex C6 (TTC-C6) containing mRNA with a 21 nt long spacer, NusA, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.90 Å |
| 6VZ2 Escherichia coli transcription-translation complex D1 (TTC-D1) containing mRNA with a 27 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 10.00 Å |
| 6VZ5 Escherichia coli transcription-translation complex D3 (TTC-D3) containing mRNA with a 21 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.90 Å |
| 6VZJ Escherichia coli transcription-translation complex A1 (TTC-A1) containing mRNA with a 15 nt long spacer, fMet-tRNAs at E-site and P-site, and lacking transcription factor NusG Deposited 2020-02-28 | Assembly 1 Other combination Heteromer；Protein × 57 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 6X6T Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B1 (TTC-B1) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-29 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6X7F Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B2 (TTC-B2) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-29 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6X7K Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-30 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6X9Q Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 27 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-06-03 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 6XDQ Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 30 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-06-11 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6XDR Escherichia coli transcription-translation complex B (TTC-B) containing an 27 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-11 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å |
| 6XGF Escherichia coli transcription-translation complex B (TTC-B) containing an 30 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-17 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.00 Å |
| 6XII Escherichia coli transcription-translation complex B (TTC-B) containing an 24 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-20 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 6XIJ Escherichia coli transcription-translation complex A (TTC-A) containing an 24 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-20 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.00 Å |
| 7K00 Structure of the Bacterial Ribosome at 2 Angstrom Resolution Deposited 2020-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 309 SPD SPERMIDINE × 16 SPM SPERMINE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.98 Å |
| 7S1G wild-type Escherichia coli stalled ribosome with antibiotic linezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain z
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 196 ZLD N-{[(5S)-3-(3-fluoro-4-morpholin-4-ylphenyl)-2-oxo-1,3-oxazolidin-5-yl]methyl}acetamide × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.48 Å |
| 7S1H Wild-type Escherichia coli ribosome with antibiotic linezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain z
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 206 ZLD N-{[(5S)-3-(3-fluoro-4-morpholin-4-ylphenyl)-2-oxo-1,3-oxazolidin-5-yl]methyl}acetamide × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.35 Å |
| 7S1I Wild-type Escherichia coli stalled ribosome with antibiotic radezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain z
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 209 RD8 Radezolid × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.48 Å |
| 7S1J Wild-type Escherichia coli ribosome with antibiotic radezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain z
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 199 RD8 Radezolid × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.47 Å |
| 7S1K Cfr-modified Escherichia coli stalled ribosome with antibiotic radezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain z
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 207 RD8 Radezolid × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.42 Å |
| 7SA4 Damaged 70S ribosome with PrfH bound Deposited 2021-09-22 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain w
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 439 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.55 Å |
| 7UG7 70S ribosome complex in an intermediate state of translocation bound to EF-G(GDP) stalled by Argyrin B Deposited 2022-03-24 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain SR
1–75（75 aa）
|
Not recorded | PUT 1,4-DIAMINOBUTANE × 11 MG MAGNESIUM ION × 404 SPD SPERMIDINE × 2 ZN ZINC ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.58 Å |
| 8EIU E. coli 70S ribosome with A-loop mutations U2554C and U2555C Deposited 2022-09-15 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 2 PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 268 SPD SPERMIDINE × 16 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 SPM SPERMINE × 1 MET METHIONINE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.24 Å |
| 8EKC Escherichia coli 70S ribosome bound to thermorubin, deacylated P-site tRNAfMet and aminoacylated A-site Phe-tRNA Deposited 2022-09-20 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain r
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 714 T8B Thermorubin × 1 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8EMM Composite 70S ribosome structure for "Atomistic simulations of the E. coli ribosome provide selection criteria for translationally active substrates Deposited 2022-09-28 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 321 SPD SPERMIDINE × 17 SPM SPERMINE × 1 K POTASSIUM ION × 10 ZN ZINC ION × 2 MET METHIONINE × 2 PAR PAROMOMYCIN × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.10 Å |
| 8EYQ 30S_delta_ksgA_h44_inactive_conformation Deposited 2022-10-28 | Assembly 1 Protein–RNA Heteromer；Protein × 17 PDB declaration: octadecameric（18） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8EYT 30S_delta_ksgA+KsgA complex Deposited 2022-10-28 | Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 21-meric（21） Consistent with all polymers |
Chain V
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 8FIZ Cryo-EM structure of E. coli 70S Ribosome containing mRNA and tRNA (in the transcription-translation complex) Deposited 2022-12-18 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain AT
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8FTO E. coli 70S ribosome with an improved MS2 tag inserted in H98 Deposited 2023-01-12 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 284 FME N-FORMYLMETHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.85 Å |
| 8FZD Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with apoRF3, RF1, P- and E-site tRNAPhe (Composite state I-B) Deposited 2023-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain r
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 581 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8FZE Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF1, P- and E-site tRNAPhe (State I-A) Deposited 2023-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain r
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 570 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8FZF Cryo-EM structure of an E. coli rotated ribosome complex bound with RF3-ppGpp and p/E-tRNAPhe (Composite state I-C) Deposited 2023-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain r
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 384 G4P GUANOSINE-5',3'-TETRAPHOSPHATE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8FZG Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF3-GDPCP, RF1, P- and E-site tRNAPhe (Composite state II-A) Deposited 2023-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain r
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 428 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8FZH Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF1, P- and E-site tRNAPhe (State II-D) Deposited 2023-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain r
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 595 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8FZI Cryo-EM structure of an E. coli rotated ribosome bound with RF3-GDPCP and p/E-tRNAPhe (Composite state II-B) Deposited 2023-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain r
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 737 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8FZJ Cryo-EM structure of an E. coli rotated ribosome bound with RF3-GDPCP and p/E-tRNAPhe (Composite state II-C) Deposited 2023-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain r
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 757 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8G6W Structure of WT E.coli 70S ribosome complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site ortho-aminobenzoic acid charged NH-tRNAPhe Deposited 2023-02-16 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 2 PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 320 2AE 2-AMINO-BENZAMIDE × 1 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 1 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 14 SPD SPERMIDINE × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.02 Å |
| 8G7P Structure of the Escherichia coli 70S ribosome in complex with EF-Tu and Ile-tRNAIle(LAU) bound to the cognate AUA codon (Structure I) Deposited 2023-02-16 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain r
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 876 K POTASSIUM ION × 2 ILE ISOLEUCINE × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8G7Q Structure of the Escherichia coli 70S ribosome in complex with EF-Tu and Ile-tRNAIle(LAU) bound to the near-cognate AUG codon (Structure II) Deposited 2023-02-16 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain r
1–75（75 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 950 ILE ISOLEUCINE × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8G7R Structure of the Escherichia coli 70S ribosome in complex with A-site tRNAIle(LAU) bound to the cognate AUA codon (Structure III) Deposited 2023-02-16 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain r
1–75（75 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 734 K POTASSIUM ION × 2 ILE ISOLEUCINE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 8G7S Structure of the Escherichia coli 70S ribosome in complex with P-site tRNAIle(LAU) bound to the cognate AUA codon (Structure IV) Deposited 2023-02-16 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain r
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 564 ILE ISOLEUCINE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8SYL Cryo-EM structure of the Escherichia coli 70S ribosome in complex with amikacin, mRNA, and A-, P-, and E-site tRNAs Deposited 2023-05-25 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain r
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 474 AKN (2S)-N-[(1R,2S,3S,4R,5S)-4-[(2R,3R,4S,5S,6R)-6-(aminomethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-5-azanyl-2-[(2S,3R,4S,5S ,6R)-4-azanyl-6-(hydroxymethyl)-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-cyclohexyl]-4-azanyl-2-oxidanyl-butanamide × 2 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8UPO Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-23 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.50 Å |
| 8UPR Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-23 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.30 Å |
| 8UQL Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8UQM Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.30 Å |
| 8UQP Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8UR0 Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-25 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8URH Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-26 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.70 Å |
| 8URI Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-26 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.30 Å |
| 8URM E. coli 70S ribosome with unmodified tRNAPro(GGG) bound to slippery P-site CCC-C codon and tRNAVal(UAC) in the A site Deposited 2023-10-26 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain w
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 395 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8URX Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 30 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-27 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 8URY Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 30 nt long spacer, and fMet-tRNA in E-site and P-site of the ribosome Deposited 2023-10-27 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8UTJ E. coli 70S ribosome with unmodified lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the 0 frame Deposited 2023-10-31 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain w
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 530 LYS LYSINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8UX8 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the +1 mRNA reading frame Deposited 2023-11-09 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain w
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 395 LYS LYSINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8UXB E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-09 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain w
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 287 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8UZ3 E. coli 70S ribosome with unmodified e*/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-14 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain w
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 415 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8UZG E. coli 70S ribosome with unmodified e*/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-15 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain w
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 398 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 8V03 E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-16 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain w
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 281 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 8VKV Escherichia coli transcription-translation coupled complex (TTC-B) containing mRNA with a 33 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-10 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8VL1 Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 36 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-11 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.30 Å |
| 8VOO Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 39 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8VOP Escherichia coli transcription-translation coupled complex (TTC-B) containing mRNA with a 36 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.00 Å |
| 8VOQ Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 39 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.30 Å |
| 8VOR Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 51 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8VOS Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 60 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 8VS9 Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site and deacyl-tRNA in the E site of E. coli 70S ribosome Deposited 2024-01-23 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain S18
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 8VSA Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site of E. coli 70S ribosome Deposited 2024-01-23 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain S18
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 8VV4 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon Deposited 2024-01-30 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain w
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 424 LYS LYSINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8VWQ E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the closed conformation) Deposited 2024-02-02 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain w
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 586 LYS LYSINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8W2N E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the open conformation) Deposited 2024-02-20 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain w
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 481 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9AX7 70S initiation complex (tRNA-fMet M1 + CUG start codon) Deposited 2024-03-06 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.63 Å |
| 9B4Z E. coli 70S ribosome complex (N1-methylated 16S A1408 + arbekacin) Deposited 2024-03-21 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain AR
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 9B50 E. coli 70S ribosome complex (unmethylated 16S A1408 + arbekacin) Deposited 2024-03-21 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain AR
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9B51 E. coli 70S ribosome complex (N1-methylated 16S A1408 + G418) Deposited 2024-03-21 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain AR
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 309 GET GENETICIN × 2 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 9CG5 70S initiation complex (tRNA-fMet M1 + UUG start codon) Deposited 2024-06-28 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.59 Å |
| 9CG6 70S initiation complex (tRNA-fMet M1 + GUG start codon) Deposited 2024-06-28 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.61 Å |
| 9CG7 70S initiation complex (tRNA-fMet M1 + AUG start codon) Deposited 2024-06-28 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.75 Å |
| 9DUK Structure of mutant 30S subunit with extended helix 26, version 3 Deposited 2024-10-03 | Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 21-meric（21） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.56 Å |
| 9DUL Structure of mutant 30S subunit with extended helix 26, version 4 Deposited 2024-10-03 | Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 21-meric（21） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.56 Å |
| 9MKK Structure of arbekacin bound Escherichia coli 70S ribosome Deposited 2024-12-17 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | 84G Arbekacin × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9MOR Damaged 70S ribosome with PrfH bound Deposited 2024-12-27 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain w
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 439 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.65 Å |
| 9MQ4 Damaged 70S ribosome with PrfH bound Deposited 2025-01-01 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain w
1–75（75 aa）
|
Not recorded | MG MAGNESIUM ION × 439 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.78 Å |
| 9O2X Structure of WT E.coli ribosome 70S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site (S)-betahydroxyBocK charged NH-tRNAPyl Deposited 2025-04-04 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 320 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 10 PAR PAROMOMYCIN × 1 SPD SPERMIDINE × 14 SPM SPERMINE × 1 A1B71 (2S)-6-[(tert-butoxycarbonyl)amino]-2-(hydroxymethyl)hexanoic acid × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.29 Å |
| 9O2Y Structure of WT E.coli ribosome 70S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site (R) beta-2-hydroxy-BocLysine acid charged NH-tRNAPyl Deposited 2025-04-04 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 320 SPD SPERMIDINE × 13 FME N-FORMYLMETHIONINE × 1 SPM SPERMINE × 1 K POTASSIUM ION × 10 ZN ZINC ION × 2 A1B70 (2R)-6-[(tert-butoxycarbonyl)amino]-2-(hydroxymethyl)hexanoic acid × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.14 Å |
| 9PIH E. coli 70S ribosome bound to Doxycycline Deposited 2025-07-10 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 276 K POTASSIUM ION × 92 DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 9PII E. coli 70S ribosome bound to Sarecycline Deposited 2025-07-10 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 281 K POTASSIUM ION × 100 V7A Sarecycline × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 9PIJ E. coli 70S ribosome bound to Minocycline Deposited 2025-07-10 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 340 K POTASSIUM ION × 148 MIY (4S,4AS,5AR,12AS)-4,7-BIS(DIMETHYLAMINO)-3,10,12,12A-TETRAHYDROXY-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2- CARBOXAMIDE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 9Y79 Escherichia coli transcription-translation loosely coupled complex (TTC-LC^walked) containing mRNA with a 39 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2025-09-09 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain C
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 3.20 Å |