Current Protein Identity:A0A140N5A3 New Search
Main Difference Dimensions in This Set
Different construct Different assembly state Different ligand/ion Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
6QDW Cryo-EM structure of the 50S ribosomal subunit at 2.83 Angstroms with modeled GBC SecM peptide Deposited 2019-01-03 Assembly 1 Protein–RNA Heteromer;Protein × 29 PDB declaration: 32-meric(32) Consistent with all polymers
Chain f 1–179(179 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5;Tico Buffer
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.83 Å
6YS3 Cryo-EM structure of the 50S ribosomal subunit at 2.58 Angstroms with modeled GBC SecM peptide Deposited 2020-04-20 Assembly 1 Protein–RNA Heteromer;Protein × 29 PDB declaration: 32-meric(32) Consistent with all polymers
Chain f 1–179(179 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.58 Å
7OTC Cryo-EM structure of an Escherichia coli 70S ribosome in complex with elongation factor G and the antibiotic Argyrin B Deposited 2021-06-10 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 52-meric(52) Consistent with all polymers
Chain F 2–178(177 aa)
Not recorded MG MAGNESIUM ION × 292 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 PUT 1,4-DIAMINOBUTANE × 8 SPD SPERMIDINE × 2 ZN ZINC ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 1I7 Argyrin B × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8FZD Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with apoRF3, RF1, P- and E-site tRNAPhe (Composite state I-B) Deposited 2023-01-28 Assembly 1 Protein–RNA Heteromer;Protein × 53 PDB declaration: 59-meric(59) Consistent with all polymers
Chain F 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 581 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4;5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZE Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF1, P- and E-site tRNAPhe (State I-A) Deposited 2023-01-28 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 58-meric(58) Consistent with all polymers
Chain F 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 570 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4;5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8FZF Cryo-EM structure of an E. coli rotated ribosome complex bound with RF3-ppGpp and p/E-tRNAPhe (Composite state I-C) Deposited 2023-01-28 Assembly 1 Protein–RNA Heteromer;Protein × 51 PDB declaration: 56-meric(56) Consistent with all polymers
Chain F 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 384 G4P GUANOSINE-5',3'-TETRAPHOSPHATE × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4;5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8FZG Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF3-GDPCP, RF1, P- and E-site tRNAPhe (Composite state II-A) Deposited 2023-01-28 Assembly 1 Protein–RNA Heteromer;Protein × 53 PDB declaration: 59-meric(59) Consistent with all polymers
Chain F 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 428 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4;5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZH Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF1, P- and E-site tRNAPhe (State II-D) Deposited 2023-01-28 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 58-meric(58) Consistent with all polymers
Chain F 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4;5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8FZI Cryo-EM structure of an E. coli rotated ribosome bound with RF3-GDPCP and p/E-tRNAPhe (Composite state II-B) Deposited 2023-01-28 Assembly 1 Protein–RNA Heteromer;Protein × 51 PDB declaration: 56-meric(56) Consistent with all polymers
Chain F 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 737 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4;5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZJ Cryo-EM structure of an E. coli rotated ribosome bound with RF3-GDPCP and p/E-tRNAPhe (Composite state II-C) Deposited 2023-01-28 Assembly 1 Protein–RNA Heteromer;Protein × 51 PDB declaration: 56-meric(56) Consistent with all polymers
Chain F 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 757 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4;5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8URM E. coli 70S ribosome with unmodified tRNAPro(GGG) bound to slippery P-site CCC-C codon and tRNAVal(UAC) in the A site Deposited 2023-10-26 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 55-meric(55) Consistent with all polymers
Chain E 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 395 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8URY Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 30 nt long spacer, and fMet-tRNA in E-site and P-site of the ribosome Deposited 2023-10-27 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain n 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UTJ E. coli 70S ribosome with unmodified lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the 0 frame Deposited 2023-10-31 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain E 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 530 LYS LYSINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8UX8 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the +1 mRNA reading frame Deposited 2023-11-09 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain E 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 395 LYS LYSINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8UXB E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-09 Assembly 1 Protein–RNA Heteromer;Protein × 48 PDB declaration: 53-meric(53) Consistent with all polymers
Chain E 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 287 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8UZ3 E. coli 70S ribosome with unmodified e*/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-14 Assembly 1 Protein–RNA Heteromer;Protein × 48 PDB declaration: 53-meric(53) Consistent with all polymers
Chain E 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 415 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8UZG E. coli 70S ribosome with unmodified e*/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-15 Assembly 1 Protein–RNA Heteromer;Protein × 48 PDB declaration: 53-meric(53) Consistent with all polymers
Chain E 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 398 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8V03 E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-16 Assembly 1 Protein–RNA Heteromer;Protein × 48 PDB declaration: 53-meric(53) Consistent with all polymers
Chain E 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 281 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
8VKV Escherichia coli transcription-translation coupled complex (TTC-B) containing mRNA with a 33 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-10 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain n 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8VL1 Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 36 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-11 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain n 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.30 Å
8VOO Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 39 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain n 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8VOP Escherichia coli transcription-translation coupled complex (TTC-B) containing mRNA with a 36 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain n 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.00 Å
8VOQ Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 39 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain n 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.30 Å
8VOR Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 51 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain n 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8VS9 Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site and deacyl-tRNA in the E site of E. coli 70S ribosome Deposited 2024-01-23 Assembly 1 Protein–RNA Heteromer;Protein × 53 PDB declaration: 59-meric(59) Consistent with all polymers
Chain L05 1–179(179 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8VSA Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site of E. coli 70S ribosome Deposited 2024-01-23 Assembly 1 Protein–RNA Heteromer;Protein × 53 PDB declaration: 58-meric(58) Consistent with all polymers
Chain L05 1–179(179 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8VV4 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon Deposited 2024-01-30 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers
Chain E 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 424 LYS LYSINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8VWQ E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the closed conformation) Deposited 2024-02-02 Assembly 1 Protein–RNA Heteromer;Protein × 51 PDB declaration: 56-meric(56) Consistent with all polymers
Chain E 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 586 LYS LYSINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8W2N E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the open conformation) Deposited 2024-02-20 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain E 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 481 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9AX7 70S initiation complex (tRNA-fMet M1 + CUG start codon) Deposited 2024-03-06 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers
Chain f 1–179(179 aa)
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9B4Z E. coli 70S ribosome complex (N1-methylated 16S A1408 + arbekacin) Deposited 2024-03-21 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 56-meric(56) Consistent with all polymers
Chain BF 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9B50 E. coli 70S ribosome complex (unmethylated 16S A1408 + arbekacin) Deposited 2024-03-21 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 52-meric(52) Consistent with all polymers
Chain BF 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
9B51 E. coli 70S ribosome complex (N1-methylated 16S A1408 + G418) Deposited 2024-03-21 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 56-meric(56) Consistent with all polymers
Chain BF 1–179(179 aa)
Not recorded MG MAGNESIUM ION × 309 GET GENETICIN × 2 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
9CG5 70S initiation complex (tRNA-fMet M1 + UUG start codon) Deposited 2024-06-28 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers
Chain f 1–179(179 aa)
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.59 Å
9CG6 70S initiation complex (tRNA-fMet M1 + GUG start codon) Deposited 2024-06-28 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers
Chain f 1–179(179 aa)
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.61 Å
9CG7 70S initiation complex (tRNA-fMet M1 + AUG start codon) Deposited 2024-06-28 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers
Chain f 1–179(179 aa)
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.75 Å
9GR1 E. coli 70S-TEC complex in delivery state Deposited 2024-09-10 Assembly 1 Protein–RNA Heteromer;Protein × 48 PDB declaration: 55-meric(55) Consistent with all polymers
Chain f 1–179(179 aa)
Not recorded ZN ZINC ION × 1 K POTASSIUM ION × 168 MG MAGNESIUM ION × 360 PHE PHENYLALANINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.17 Å