Current Protein Identity:B7MCR3 New Search
Main Difference Dimensions in This Set
Different construct Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
11EL Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Vibrio cholerae (VC-ST) Deposited 2026-02-19 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 53-meric(53) Consistent with all polymers
Chain K 1–129(129 aa)
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 308 SPD SPERMIDINE × 16 SPM SPERMINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
11FV Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Pseudomonas aeruginosa (PA-S3.3) Deposited 2026-02-21 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 52-meric(52) Consistent with all polymers
Chain r 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 307 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
11GG Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Pseudomonas aeruginosa (PA-ST) Deposited 2026-02-22 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 52-meric(52) Consistent with all polymers
Chain r 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 307 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.02 Å
5U9F 3.2 A cryo-EM ArfA-RF2 ribosome rescue complex (Structure II) Deposited 2016-12-16 Assembly 1 Protein–RNA Heteromer;Protein × 54 PDB declaration: 60-meric(60) Consistent with all polymers
Chain K 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 360 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
5U9G 3.2 A cryo-EM ArfA-RF2 ribosome rescue complex (Structure I) Deposited 2016-12-16 Assembly 1 Protein–RNA Heteromer;Protein × 54 PDB declaration: 60-meric(60) Consistent with all polymers
Chain K 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 348 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
5UZ4 The cryo-EM structure of YjeQ bound to the 30S subunit suggests a fidelity checkpoint function for this protein in ribosome assembly Deposited 2017-02-24 Assembly 1 Protein–RNA Heteromer;Protein × 20 PDB declaration: 21-meric(21) Consistent with all polymers
Chain K 1–129(129 aa)
Not recorded ZN ZINC ION × 1 GGM 3'-O-(N-methylanthraniloyl)-beta:gamma-imidoguanosine-5'-triphosphate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5WF0 70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex C2) Deposited 2017-07-11 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 59-meric(59) Consistent with all polymers
Chain k 13–128(116 aa)
Not recorded FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 1480 K POTASSIUM ION × 5 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE;5 second blot time, no wait time
Resolution 3.60 Å
5WFS 70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex C4) Deposited 2017-07-12 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 59-meric(59) Consistent with all polymers
Chain k 13–128(116 aa)
Not recorded FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 1471 K POTASSIUM ION × 7 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE;5 second blot time, no wait time
Resolution 3.00 Å
6AWB Structure of 30S ribosomal subunit and RNA polymerase complex in non-rotated state Deposited 2017-09-05 Assembly 1 Protein–RNA Heteromer;Protein × 26 PDB declaration: 27-meric(27) Consistent with all polymers
Chain N 13–128(116 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7;20 mM Tris-HCl, pH 7.0, 100 mM NH4Cl, 10 mM MgCl2, 0.5 mM EDTA, 6 mM BME
cryo-EM vitrification conditions Cryogen ETHANE;2.5 uL of 50 nM 30S and 150 nM RNAP was applied to the grid. After a 30 second incubation, the grid was blotted for 5 seconds at blotting power 8.
Resolution 6.70 Å
6AWC Structure of 30S ribosomal subunit and RNA polymerase complex in rotated state Deposited 2017-09-05 Assembly 1 Protein–RNA Heteromer;Protein × 26 PDB declaration: 27-meric(27) Consistent with all polymers
Chain N 13–128(116 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7;20 mM Tris-HCl, pH 7.0, 100 mM NH4Cl, 10 mM MgCl2, 0.5 mM EDTA, 6 mM BME
cryo-EM vitrification conditions Cryogen ETHANE;2.5 uL of 50 nM 30S and 150 nM RNAP was applied to the grid. After a 30 second incubation, the grid was blotted for 5 seconds at blotting power 8.
Resolution 7.90 Å
6AWD Structure of 30S (S1 depleted) ribosomal subunit and RNA polymerase complex Deposited 2017-09-05 Assembly 1 Protein–RNA Heteromer;Protein × 25 PDB declaration: 26-meric(26) Consistent with all polymers
Chain N 13–128(116 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7;20 mM Tris-HCl, pH 7.0, 100 mM NH4Cl, 10 mM MgCl2, 0.5 mM EDTA, 6 mM BME
cryo-EM vitrification conditions Cryogen ETHANE;2.5 uL of 50 nM 30S and 150 nM RNAP was applied to the grid. After a 30 second incubation, the grid was blotted for 5 seconds at blotting power 8.
Resolution 8.10 Å
6DNC E.coli RF1 bound to E.coli 70S ribosome in response to UAU sense A-site codon Deposited 2018-06-06 Assembly 1 Protein–RNA Heteromer;Protein × 53 PDB declaration: 59-meric(59) Consistent with all polymers
Chain XA 1–129(129 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7;20 mM Tris-HCl, 100 mM NH4Cl, 20.5 mM MgCl2, 0.5 mM EDTA, 6 mM beta-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6OGF 70S termination complex with RF2 bound to the UGA codon. Partially rotated ribosome with RF2 bound (Structure III). Deposited 2019-04-02 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain P 1–129(129 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6OGG 70S termination complex with RF2 bound to the UGA codon. Rotated ribosome with RF2 bound (Structure IV). Deposited 2019-04-02 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain P 1–129(129 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6OGI 70S termination complex with RF2 bound to the UAG codon. Rotated ribosome conformation (Structure V) Deposited 2019-04-02 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers
Chain P 1–129(129 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6OM6 Structure of trans-translation inhibitor bound to E. coli 70S ribosome with P site tRNA Deposited 2019-04-18 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers
Chain p 1–129(129 aa)
Not recorded KKL 4-amino-N-[5-(4-ethynylphenyl)-1,3,4-oxadiazol-2-yl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE;Blot time: 3.5 seconds
Resolution 3.10 Å
6VYQ Escherichia coli transcription-translation complex A1 (TTC-A1) containing an 15 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 58 PDB declaration: 66-meric(66) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6VYR Escherichia coli transcription-translation complex A1 (TTC-A1) containing an 18 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 58 PDB declaration: 66-meric(66) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6VYS Escherichia coli transcription-translation complex A1 (TTC-A1) containing a 21 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 58 PDB declaration: 66-meric(66) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6VYT Escherichia coli transcription-translation complex A2 (TTC-A2) containing a 15 nt long mRNA spacer, NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 55 PDB declaration: 64-meric(64) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 14.00 Å
6VYU Escherichia coli transcription-translation complex C2 (TTC-C2) containing a 27 nt long mRNA spacer Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.00 Å
6VYW Escherichia coli transcription-translation complex C3 (TTC-C3) containing mRNA with a 27 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.00 Å
6VYX Escherichia coli transcription-translation complex C4 (TTC-C4) containing mRNA with a 21 nt long spacer, transcription factor NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.90 Å
6VYY Escherichia coli transcription-translation complex C5 (TTC-C5) containing mRNA with a 21 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.90 Å
6VYZ Escherichia coli transcription-translation complex C6 (TTC-C6) containing mRNA with a 21 nt long spacer, NusA, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.90 Å
6VZ2 Escherichia coli transcription-translation complex D1 (TTC-D1) containing mRNA with a 27 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 10.00 Å
6X6T Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B1 (TTC-B1) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-29 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6X7F Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B2 (TTC-B2) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-29 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
6X7K Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-30 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6X9Q Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 27 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-06-03 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
6XDQ Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 30 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-06-11 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6XDR Escherichia coli transcription-translation complex B (TTC-B) containing an 27 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-11 Assembly 1 Other combination Heteromer;Protein × 59 PDB declaration: 67-meric(67) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.70 Å
6XGF Escherichia coli transcription-translation complex B (TTC-B) containing an 30 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-17 Assembly 1 Other combination Heteromer;Protein × 59 PDB declaration: 67-meric(67) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
6XII Escherichia coli transcription-translation complex B (TTC-B) containing an 24 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-20 Assembly 1 Other combination Heteromer;Protein × 59 PDB declaration: 67-meric(67) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.00 Å
6XIJ Escherichia coli transcription-translation complex A (TTC-A) containing an 24 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-20 Assembly 1 Other combination Heteromer;Protein × 58 PDB declaration: 66-meric(66) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.00 Å
7S1G wild-type Escherichia coli stalled ribosome with antibiotic linezolid Deposited 2021-09-02 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain r 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 196 ZLD N-{[(5S)-3-(3-fluoro-4-morpholin-4-ylphenyl)-2-oxo-1,3-oxazolidin-5-yl]methyl}acetamide × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.48 Å
7S1H Wild-type Escherichia coli ribosome with antibiotic linezolid Deposited 2021-09-02 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 52-meric(52) Consistent with all polymers
Chain r 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 206 ZLD N-{[(5S)-3-(3-fluoro-4-morpholin-4-ylphenyl)-2-oxo-1,3-oxazolidin-5-yl]methyl}acetamide × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.35 Å
7S1I Wild-type Escherichia coli stalled ribosome with antibiotic radezolid Deposited 2021-09-02 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain r 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 209 RD8 Radezolid × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.48 Å
7S1J Wild-type Escherichia coli ribosome with antibiotic radezolid Deposited 2021-09-02 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 52-meric(52) Consistent with all polymers
Chain r 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 199 RD8 Radezolid × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.47 Å
7S1K Cfr-modified Escherichia coli stalled ribosome with antibiotic radezolid Deposited 2021-09-02 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain r 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 207 RD8 Radezolid × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.42 Å
7SA4 Damaged 70S ribosome with PrfH bound Deposited 2021-09-22 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 58-meric(58) Consistent with all polymers
Chain p 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 439 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.55 Å
7UG7 70S ribosome complex in an intermediate state of translocation bound to EF-G(GDP) stalled by Argyrin B Deposited 2022-03-24 Assembly 1 Protein–RNA Heteromer;Protein × 54 PDB declaration: 60-meric(60) Consistent with all polymers
Chain SK 1–129(129 aa)
Not recorded PUT 1,4-DIAMINOBUTANE × 11 MG MAGNESIUM ION × 404 SPD SPERMIDINE × 2 ZN ZINC ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.58 Å
8EYQ 30S_delta_ksgA_h44_inactive_conformation Deposited 2022-10-28 Assembly 1 Protein–RNA Heteromer;Protein × 17 PDB declaration: octadecameric(18) Consistent with all polymers
Chain K 1–129(129 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8EYT 30S_delta_ksgA+KsgA complex Deposited 2022-10-28 Assembly 1 Protein–RNA Heteromer;Protein × 20 PDB declaration: 21-meric(21) Consistent with all polymers
Chain R 1–129(129 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8FIZ Cryo-EM structure of E. coli 70S Ribosome containing mRNA and tRNA (in the transcription-translation complex) Deposited 2022-12-18 Assembly 1 Protein–RNA Heteromer;Protein × 51 PDB declaration: 56-meric(56) Consistent with all polymers
Chain AM 1–129(129 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8TUV Merkel Cell Polyomavirus LTA NLSm bound to importin alpha 2 Deposited 2023-08-17 Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count
Chain C 1–129(129 aa)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;296 K;0.75 M sodium citrate, 0.1 M HEPES pH 6.5, 0.01 M DTT
Resolution 2.30 Å R-free 0.218
8UPO Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-23 Assembly 1 Other combination Heteromer;Protein × 58 PDB declaration: 66-meric(66) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.50 Å
8UPR Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-23 Assembly 1 Other combination Heteromer;Protein × 59 PDB declaration: 67-meric(67) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.30 Å
8UQL Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24 Assembly 1 Other combination Heteromer;Protein × 59 PDB declaration: 67-meric(67) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8UQM Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.30 Å
8UQP Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24 Assembly 1 Other combination Heteromer;Protein × 59 PDB declaration: 67-meric(67) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8UR0 Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-25 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8URH Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-26 Assembly 1 Other combination Heteromer;Protein × 59 PDB declaration: 67-meric(67) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.70 Å
8URI Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-26 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.30 Å
8URM E. coli 70S ribosome with unmodified tRNAPro(GGG) bound to slippery P-site CCC-C codon and tRNAVal(UAC) in the A site Deposited 2023-10-26 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 55-meric(55) Consistent with all polymers
Chain p 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 395 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8URX Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 30 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-27 Assembly 1 Other combination Heteromer;Protein × 59 PDB declaration: 67-meric(67) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.60 Å
8UTJ E. coli 70S ribosome with unmodified lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the 0 frame Deposited 2023-10-31 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain p 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 530 LYS LYSINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8UX8 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the +1 mRNA reading frame Deposited 2023-11-09 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain p 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 395 LYS LYSINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8UXB E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-09 Assembly 1 Protein–RNA Heteromer;Protein × 48 PDB declaration: 53-meric(53) Consistent with all polymers
Chain p 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 287 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8UZ3 E. coli 70S ribosome with unmodified e*/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-14 Assembly 1 Protein–RNA Heteromer;Protein × 48 PDB declaration: 53-meric(53) Consistent with all polymers
Chain p 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 415 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8UZG E. coli 70S ribosome with unmodified e*/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-15 Assembly 1 Protein–RNA Heteromer;Protein × 48 PDB declaration: 53-meric(53) Consistent with all polymers
Chain p 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 398 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8V03 E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-16 Assembly 1 Protein–RNA Heteromer;Protein × 48 PDB declaration: 53-meric(53) Consistent with all polymers
Chain p 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 281 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
8VKV Escherichia coli transcription-translation coupled complex (TTC-B) containing mRNA with a 33 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-10 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8VL1 Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 36 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-11 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.30 Å
8VOO Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 39 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8VOP Escherichia coli transcription-translation coupled complex (TTC-B) containing mRNA with a 36 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.00 Å
8VOQ Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 39 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.30 Å
8VOR Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 51 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8VOS Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 60 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
8VS9 Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site and deacyl-tRNA in the E site of E. coli 70S ribosome Deposited 2024-01-23 Assembly 1 Protein–RNA Heteromer;Protein × 53 PDB declaration: 59-meric(59) Consistent with all polymers
Chain S11 1–129(129 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8VSA Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site of E. coli 70S ribosome Deposited 2024-01-23 Assembly 1 Protein–RNA Heteromer;Protein × 53 PDB declaration: 58-meric(58) Consistent with all polymers
Chain S11 1–129(129 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8VV4 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon Deposited 2024-01-30 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers
Chain p 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 424 LYS LYSINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8VWQ E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the closed conformation) Deposited 2024-02-02 Assembly 1 Protein–RNA Heteromer;Protein × 51 PDB declaration: 56-meric(56) Consistent with all polymers
Chain p 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 586 LYS LYSINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8W2N E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the open conformation) Deposited 2024-02-20 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain p 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 481 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9B4Z E. coli 70S ribosome complex (N1-methylated 16S A1408 + arbekacin) Deposited 2024-03-21 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 56-meric(56) Consistent with all polymers
Chain AK 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9B50 E. coli 70S ribosome complex (unmethylated 16S A1408 + arbekacin) Deposited 2024-03-21 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 52-meric(52) Consistent with all polymers
Chain AK 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
9B51 E. coli 70S ribosome complex (N1-methylated 16S A1408 + G418) Deposited 2024-03-21 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 56-meric(56) Consistent with all polymers
Chain AK 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 309 GET GENETICIN × 2 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
9MKK Structure of arbekacin bound Escherichia coli 70S ribosome Deposited 2024-12-17 Assembly 1 Protein–RNA Heteromer;Protein × 47 PDB declaration: 52-meric(52) Consistent with all polymers
Chain K 1–129(129 aa)
Not recorded 84G Arbekacin × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9MOR Damaged 70S ribosome with PrfH bound Deposited 2024-12-27 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 58-meric(58) Consistent with all polymers
Chain p 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 439 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9MQ4 Damaged 70S ribosome with PrfH bound Deposited 2025-01-01 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 58-meric(58) Consistent with all polymers
Chain p 1–129(129 aa)
Not recorded MG MAGNESIUM ION × 439 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.78 Å
9PIH E. coli 70S ribosome bound to Doxycycline Deposited 2025-07-10 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers
Chain K 1–129(129 aa)
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 276 K POTASSIUM ION × 92 DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
9PII E. coli 70S ribosome bound to Sarecycline Deposited 2025-07-10 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers
Chain K 1–129(129 aa)
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 281 K POTASSIUM ION × 100 V7A Sarecycline × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
9PIJ E. coli 70S ribosome bound to Minocycline Deposited 2025-07-10 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers
Chain K 1–129(129 aa)
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 340 K POTASSIUM ION × 148 MIY (4S,4AS,5AR,12AS)-4,7-BIS(DIMETHYLAMINO)-3,10,12,12A-TETRAHYDROXY-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2- CARBOXAMIDE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9Y79 Escherichia coli transcription-translation loosely coupled complex (TTC-LC^walked) containing mRNA with a 39 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2025-09-09 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain Q 1–129(129 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions Resolution 3.20 Å