Current Protein Identity：G1T1F0
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain OO
21–156（136 aa）
|
Not recorded | MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions
After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
|
Resolution 3.65 Å |
| 3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain OO
21–156（136 aa）
|
Not recorded | MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions
After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
|
Resolution 3.45 Å |
| 3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain OO
21–156（136 aa）
|
Not recorded | MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions
After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
|
Resolution 3.65 Å |
| 5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain OO
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
|
Resolution 3.31 Å |
| 5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain OO
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
|
Resolution 3.65 Å |
| 5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain OO
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 258 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
|
Resolution 3.75 Å |
| 5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain OO
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
|
Resolution 3.35 Å |
| 5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain OO
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
|
Resolution 3.53 Å |
| 5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain OO
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
|
Resolution 3.67 Å |
| 5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain OO
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
|
Resolution 3.99 Å |
| 5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain OO
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
|
Resolution 3.47 Å |
| 6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain PP
1–151（151 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain PP
1–151（151 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain MM
21–155（135 aa）
|
Not recorded | MG MAGNESIUM ION × 299 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 | Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric（41） Consistent with all polymers |
Chain Q
1–151（151 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric（50） Consistent with all polymers |
Chain Q
1–151（151 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric（39） Consistent with all polymers |
Chain Q
16–151（136 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.48 Å |
| 6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain a5
16–151（136 aa）
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.49 Å |
| 7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain a5
16–151（136 aa）
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain P2
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；50 mM HEPES pH 7.4
100 mM KOAc
5 mM Mg(Oac)2
1mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
|
Resolution 3.80 Å |
| 7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain OO
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；50 mM HEPES pH 7.4
100 mM KOAc
5 mM Mg(Oac)2
1mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
|
Resolution 4.10 Å |
| 7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain An
1–151（151 aa）
|
Not recorded | SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.20 Å |
| 7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain An
1–151（151 aa）
|
Not recorded | SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.40 Å |
| 7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain An
1–151（151 aa）
|
Not recorded | SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.90 Å |
| 7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain An
1–151（151 aa）
|
Not recorded | MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.10 Å |
| 8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain P3
16–151（136 aa）
|
Not recorded | MG MAGNESIUM ION × 275 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 | Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric（40） Consistent with all polymers |
Chain Q
1–151（151 aa）
|
Not recorded | MG MAGNESIUM ION × 178 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |
| 8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 | Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric（40） Consistent with all polymers |
Chain Q
1–151（151 aa）
|
Not recorded | MG MAGNESIUM ION × 230 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9BDL 80S ribosome with angiogenin Deposited 2024-04-12 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain AS14
16–150（135 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain AS14
16–150（135 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain AS14
16–150（135 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 | Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric（35） Consistent with all polymers |
Chain O
1–151（151 aa）
|
Not recorded | MG MAGNESIUM ION × 74 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain 0
1–151（151 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 6.00 Å |
| 9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 | Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric（42） Consistent with all polymers |
Chain Q
1–151（151 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions
Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
|
Resolution 2.90 Å |
| 9H74 Late-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guided by the trans-RNA Deposited 2024-10-25 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain Q
1–151（151 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions
Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
|
Resolution 2.90 Å |
| 9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain MM
1–151（151 aa）
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 285 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.65 Å |
| 9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 | Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain MM
1–151（151 aa）
|
Not recorded | MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.82 Å |
| 9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain MM
1–151（151 aa）
|
Not recorded | MG MAGNESIUM ION × 291 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.86 Å |
| 9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain An
1–151（151 aa）
|
Not recorded | MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.80 Å |
| 9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric（89） Consistent with all polymers |
Chain An
1–151（151 aa）
|
Not recorded | SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.43 Å |
| 9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Z1
1–151（151 aa）
|
Not recorded | MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.65 Å |
| 9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain OO
1–151（151 aa）
|
Not recorded | SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain OO
1–151（151 aa）
|
Not recorded | SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |