Current Protein Identity:O75570
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 | Assembly 1 Protein–RNA Heteromer;Protein × 88 PDB declaration: 94-meric(94) Consistent with all polymers |
Chain Aa
1–445(445 aa)
|
Not recorded | MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.60 Å |
| 8OIP 28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 | Assembly 1 Protein–RNA Heteromer;Protein × 33 PDB declaration: 36-meric(36) Consistent with all polymers |
Chain Aa
1–445(445 aa)
|
Not recorded | MG MAGNESIUM ION × 127 SPM SPERMINE × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 11 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.60 Å |
| 8OIQ 39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 | Assembly 1 Protein–RNA Heteromer;Protein × 58 PDB declaration: 62-meric(62) Consistent with all polymers |
Chain Aa
1–445(445 aa)
|
Not recorded | MG MAGNESIUM ION × 205 K POTASSIUM ION × 30 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.50 Å |
| 8OIR 55S human mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 | Assembly 1 Protein–RNA Heteromer;Protein × 88 PDB declaration: 94-meric(94) Consistent with all polymers |
Chain Aa
1–445(445 aa)
|
Mutation:311GGQ313 > AAQ | K POTASSIUM ION × 51 MG MAGNESIUM ION × 354 VAL VALINE × 1 ZN ZINC ION × 3 FS2 FE-S-O HYBRID CLUSTER × 3 MET METHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.10 Å |
| 8OIS 28S human mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 | Assembly 1 Protein–RNA Heteromer;Protein × 33 PDB declaration: 36-meric(36) Consistent with all polymers |
Chain Aa
1–445(445 aa)
|
Mutation:311GGQ313 > AAQ | K POTASSIUM ION × 17 MG MAGNESIUM ION × 126 MET METHIONINE × 1 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.00 Å |
| 8OIT 39S human mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 | Assembly 1 Protein–RNA Heteromer;Protein × 58 PDB declaration: 62-meric(62) Consistent with all polymers |
Chain Aa
1–445(445 aa)
|
Mutation:311GGQ313 > AAQ | K POTASSIUM ION × 34 MG MAGNESIUM ION × 228 VAL VALINE × 1 ZN ZINC ION × 2 FS2 FE-S-O HYBRID CLUSTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.90 Å |