Current Protein Identity：P00634
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1AJA THREE-DIMENSIONAL STRUCTURE OF THE D153G MUTANT OF E. COLI ALKALINE PHOSPHATASE: A MUTANT WITH WEAKER MAGNESIUM BINDING AND INCREASED CATALYTIC ACTIVITY Deposited 1995-08-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：D153G Mutation：D153G | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.50 Å |
| 1AJB THREE-DIMENSIONAL STRUCTURE OF THE D153G MUTANT OF E. COLI ALKALINE PHOSPHATASE: A MUTANT WITH WEAKER MAGNESIUM BINDING AND INCREASED CATALYTIC ACTIVITY Deposited 1995-08-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：D153G Mutation：D153G | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.50 Å |
| 1AJC THREE-DIMENSIONAL STRUCTURE OF THE D153G MUTANT OF E. COLI ALKALINE PHOSPHATASE: A MUTANT WITH WEAKER MAGNESIUM BINDING AND INCREASED CATALYTIC ACTIVITY Deposited 1995-07-18 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：D153G Mutation：D153G | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.50 Å |
| 1AJD THREE-DIMENSIONAL STRUCTURE OF THE D153G MUTANT OF E. COLI ALKALINE PHOSPHATASE: A MUTANT WITH WEAKER MAGNESIUM BINDING AND INCREASED CATALYTIC ACTIVITY Deposited 1995-08-18 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：D153G Mutation：D153G | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.50 Å |
| 1ALH KINETICS AND CRYSTAL STRUCTURE OF A MUTANT E. COLI ALKALINE PHOSPHATASE (ASP-369-->ASN): A MECHANISM INVOLVING ONE ZINC PER ACTIVE SITE Deposited 1994-08-23 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
26–471（446 aa）
Chain B
26–471（446 aa）
|
Not recorded | ZN ZINC ION × 2 PO4 PHOSPHATE ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.50 Å |
| 1ALI ALKALINE PHOSPHATASE MUTANT (H412N) Deposited 1995-06-02 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：H412N Mutation：H412N | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
THE STRUCTURE IS OF A MUTANT ALKALINE PHOSPHATE (H412N)
IN WHICH HIS 412 IS REPLACED BY ASN, DETERMINED WITH
CRYSTALS SOAKED IN STABILIZATION BUFFER CONTAINING 10 MM
ZINC CHLORIDE. THERE ARE TWO ZINCS AND ONE MAGNESIUM
COMPLEXED WITH AN INORGANIC PHOSPHATE BOUND IN EACH OF
THE TWO ACTIVE SITES.
|
Resolution 2.20 Å |
| 1ALJ ALKALINE PHOSPHATASE MUTANT (H412N) Deposited 1995-06-02 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：H412N Mutation：H412N | ZN ZINC ION × 2 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
THIS ENTRY IS A MUTANT ALKALINE PHOSPHATE (H412N)
IN WHICH HIS 412 IS REPLACED BY ASN, DETERMINED WITH
CRYSTALS SOAKED IN STABILIZATION BUFFER CONTAINING NO
ADDED ZINC. THERE IS ONE ZINC AND ONE MAGNESIUM
COMPLEXED WITH INORGANIC PHOSPHATE BOUND IN EACH OF
THE TWO ACTIVE SITES.
|
Resolution 2.60 Å |
| 1ALK REACTION MECHANISM OF ALKALINE PHOSPHATASE BASED ON CRYSTAL STRUCTURES. TWO METAL ION CATALYSIS Deposited 1993-03-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1ANI ALKALINE PHOSPHATASE (D153H, K328H) Deposited 1995-09-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
26–471（446 aa）
Chain B
26–471（446 aa）
|
Mutation：D153H, K328H Mutation：D153H, K328H | ZN ZINC ION × 6 PO4 PHOSPHATE ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.50 Å |
| 1ANJ ALKALINE PHOSPHATASE (K328H) Deposited 1995-09-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
26–471（446 aa）
Chain B
26–471（446 aa）
|
Mutation：K328H Mutation：K328H | ZN ZINC ION × 6 PO4 PHOSPHATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.30 Å R-free 0.249 |
| 1B8J ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE Deposited 1999-02-01 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 4 SO4 SULFATE ION × 2 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；CRYSTALLIZATION CONDITIONS: 65 % SATURATING (NH4)2SO4, 100 MM TRIS, 10 MM MGCL2, 1 MM ZNCL2, 0.1 MM NH4VO3, PH 7.5
|
Resolution 1.90 Å R-free 0.196 |
| 1ED8 STRUCTURE OF E. COLI ALKALINE PHOSPHATASE INHIBITED BY THE INORGANIC PHOSPHATE AT 1.75A RESOLUTION Deposited 2000-01-27 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Tris, ammonium sulfate, magnesium chloride, zinc chloride , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.75 Å R-free 0.228 |
| 1ED9 STRUCTURE OF E. COLI ALKALINE PHOSPHATASE WITHOUT THE INORGANIC PHOSPHATE AT 1.75A RESOLUTION Deposited 2000-01-27 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Tris, ammonium sulfate,
magnesium chloride, zinc chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.75 Å R-free 0.224 |
| 1ELX E. COLI ALKALINE PHOSPHATASE MUTANT (S102A) Deposited 1998-02-10 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：S102A Mutation：S102A | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；25 MG/ML PROTEIN IN 39% SATURATING (NH4)2SO4, 100 MM TRIS, 10 MM MGCL2 100 MM ZNCL2, 2 MM NAH2PO4 AT PH 7.5, EQUILIBRATED AGAINST 55% SATURATING (NH4)2SO4
|
Resolution 2.60 Å R-free 0.207 |
| 1ELY E. COLI ALKALINE PHOSPHATASE MUTANT (S102C) Deposited 1998-02-10 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：S102C Mutation：S102C | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；25 MG/ML PROTEIN IN 39% SATURATING (NH4)2SO4, 100 MM TRIS, 10 MM MGCL2 100 MM ZNCL2, 2 MM NAH2PO4 AT PH 7.5, EQUILIBRATED AGAINST 55% SATURATING (NH4)2SO4
|
Resolution 2.80 Å R-free 0.193 |
| 1ELZ E. COLI ALKALINE PHOSPHATASE MUTANT (S102G) Deposited 1998-02-10 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：S102G Mutation：S102G | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；25 MG/ML PROTEIN IN 39% SATURATING (NH4)2SO4, 100 MM TRIS, 10 MM MGCL2 100 MM ZNCL2, 2 MM NAH2PO4 AT PH 7.5, EQUILIBRATED AGAINST 55% SATURATING (NH4)2SO4
|
Resolution 2.80 Å R-free 0.173 |
| 1EW8 ALKALINE PHOSPHATASE (E.C. 3.1.3.1) COMPLEX WITH PHOSPHONOACETIC ACID Deposited 2000-04-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Not recorded | ZN ZINC ION × 6 PO4 PHOSPHATE ION × 2 SO4 SULFATE ION × 2 MG MAGNESIUM ION × 2 PAE PHOSPHONOACETIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9.5；298 K；enzyme: 30 mg/mL; buffer: 40% saturating ammonium sulfate/100 mM Tris/10 mM MgSO4, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.20 Å R-free 0.257 |
| 1EW9 ALKALINE PHOSPHATASE (E.C. 3.1.3.1) COMPLEX WITH MERCAPTOMETHYL PHOSPHONATE Deposited 2000-04-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Not recorded | ZN ZINC ION × 6 SO4 SULFATE ION × 2 MG MAGNESIUM ION × 2 MMQ MERCAPTOMETHYL PHOSPHONATE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9.5；298 K；enzyme: 30 mg/mL; buffer: 40% saturated ammonium sulfate, 100 mM Tris/10 mM magnesium sulfate, pH 9.5, VAPOR
DIFFUSION, HANGING DROP, temperature 25K
|
Resolution 2.00 Å R-free 0.229 |
| 1HJK ALKALINE PHOSPHATASE MUTANT H331Q Deposited 1997-05-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：H331Q Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：H331Q Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；55% SATURATING (NH4)2SO4, 100 MM TRIS, 10 MM MGCL2, 10 MM ZNCL2, 2 MM NAH2PO4 AT PH 7.5
|
Resolution 2.30 Å R-free 0.200 |
| 1HQA ALKALINE PHOSPHATASE (H412Q) Deposited 1995-11-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：H412Q Mutation：H412Q | ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.25 Å |
| 1KH4 E. COLI ALKALINE PHOSPHATASE MUTANT (D330N) IN COMPLEX WITH PHOSPHATE Deposited 2001-11-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：D330N Mutation：D330N | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；Ammonium sulfate, magnesium chloride, zinc sulfate, TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.245 |
| 1KH5 E. COLI ALKALINE PHOSPHATASE MUTANT (D330N) MIMIC OF THE TRANSITION STATES WITH ALUMINIUM FLUORIDE Deposited 2001-11-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：D330N Mutation：D330N | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 AF3 ALUMINUM FLUORIDE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；292 K；Ammonium sulfate, magnesium chloride, zinc sulfate, TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.00 Å R-free 0.228 |
| 1KH7 E. COLI ALKALINE PHOSPHATASE MUTANT (D153GD330N) Deposited 2001-11-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：D153G, D330N Mutation：D153G, D330N | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；292 K；ammonium sulfate, magnesium chloride, zinc sulfate, TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.40 Å R-free 0.249 |
| 1KH9 E. COLI ALKALINE PHOSPHATASE MUTANT (D153GD330N) COMPLEX WITH PHOSPHATE Deposited 2001-11-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：D153G, D330N Mutation：D153G, D330N | ZN ZINC ION × 4 MG MAGNESIUM ION × 1 PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；292 K；ammonium sulfate, magnesium chloride, zinc sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.50 Å R-free 0.250 |
| 1KHJ E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N) MIMIC OF THE TRANSITION STATES WITH ALUMINIUM FLUORIDE Deposited 2001-11-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：D153H, D330N Mutation：D153H, D330N | ZN ZINC ION × 4 AF3 ALUMINUM FLUORIDE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；292 K；ammonium sulfate, magnesium chloride, zinc sulfate, TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.30 Å R-free 0.223 |
| 1KHK E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N) Deposited 2001-11-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：D153H, D330N Mutation：D153H, D330N | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；292 K；ammonium sulfate, magnesium chloride, zinc sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.50 Å R-free 0.207 |
| 1KHL E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N) COMPLEX WITH PHOSPHATE Deposited 2001-11-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：D153H, D330N Mutation：D153H, D330N | ZN ZINC ION × 4 PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；292 K；ammonium sulfate, magnesium chloride, zinc sulfate, TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.50 Å R-free 0.227 |
| 1KHN E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N) ZINC FORM Deposited 2001-11-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：D153H, D330N Mutation：D153H, D330N | ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；292 K；ammonium sulfate, magnesium chloride, zinc sulfate, TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.60 Å R-free 0.248 |
| 1URA ALKALINE PHOSPHATASE (D51ZN) Deposited 1996-02-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
26–471（446 aa）
Chain B
26–471（446 aa）
|
Mutation：D51N Mutation：D51N | ZN ZINC ION × 4 PO4 PHOSPHATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；65% SATURATING (NH4)2SO4, 100 MM TRIS, 10 MM MGCL2, 10 MM ZNCL2, 2 MM NAH2PO4 AT PH 7.5.
|
Resolution 2.04 Å R-free 0.234 |
| 1URB ALKALINE PHOSPHATASE (N51MG) Deposited 1996-02-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
26–471（446 aa）
Chain B
26–471（446 aa）
|
Mutation：D51N Mutation：D51N | ZN ZINC ION × 2 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 9.2；45% SATURATING (NH4)2SO4, 0.10 MM CAPS, 20 MM MGCL2, 10 UM ZNCL2, 0.1 MM NAH2PO4 AT PH 9.2.
|
Resolution 2.14 Å R-free 0.241 |
| 1Y6V Structure of E. coli Alkaline Phosphatase in presence of cobalt at 1.60 A resolution Deposited 2004-12-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Not recorded | CO COBALT (II) ION × 6 PO4 PHOSPHATE ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9.5；293 K；10 mM cobalt chloride, 2.1 M Ammonium sulfate, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.60 Å R-free 0.212 |
| 1Y7A Structure of D153H/K328W E. coli alkaline phosphatase in presence of cobalt at 1.77 A resolution Deposited 2004-12-08 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：D153H, K328W Mutation：D153H, K328W | CO COBALT (II) ION × 6 PO4 PHOSPHATE ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9.5；293 K；10 mM cobalt chloride, 2.2 M ammonium sulfate, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.77 Å R-free 0.238 |
| 2ANH ALKALINE PHOSPHATASE (D153H) Deposited 1995-09-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
26–471（446 aa）
Chain B
26–471（446 aa）
|
Mutation：D153H Mutation：D153H | ZN ZINC ION × 6 PO4 PHOSPHATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.40 Å R-free 0.239 |
| 2G9Y Structure of S102T E. coli alkaline phosphatase in presence of phosphate at 2.00 A resolution Deposited 2006-03-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：S102T Mutation：S102T | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9.5；293 K；2.0M ammonium sulfate, 100mM Tris, 10mM magnesium chloride, 0.01mM zinc chloride, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.242 |
| 2GA3 Structure of S102T E. coli Alkaline Phosphatase-phosphate intermediate at 2.20A resolution Deposited 2006-03-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：S102(TPO) Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：S102(TPO) Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9.5；293 K；2.0M ammonium sulfate, 100mM Tris, 10mM magnesium chloride, 0.01mM zinc chloride, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.229 |
| 2MLZ NMR structure of E. coli Trigger Factor in complex with unfolded PhoA365-471 Deposited 2014-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
360–471（112 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；295 K；Ionic strength (raw mmCIF value) 100；Pressure ambient
NMR sample composition
0.5 mM [U-100% 13C; U-100% 15N], Alkaline phosphatase, 0.5 mM [U-100% 13C; U-100% 15N], Trigger Factor, 100 mM potassium chloride, 3 mM BME, 20 mM potassium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 3BDF Crystal structure of metal-free E. coli alkaline phosphatase (T155V) Deposited 2007-11-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
22–471（450 aa）
Chain B
22–471（450 aa）
|
Mutation：T155V Mutation：T155V | SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1 M MES, 0.2 M LiSO4, 1 mM ZnCl2, 5 mM MgCl2, 25-30% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.40 Å R-free 0.184 |
| 3BDG Crystal structure of wild-type/T155V mixed dimer of E. coli alkaline phosphatase Deposited 2007-11-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
22–471（450 aa）
Chain B
22–471（450 aa）
|
Mutation：T155V | SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1 M MES, 0.2 M LiSO4, 1 mM ZnCl2, 5 mM MgCl2, 25-30% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.40 Å R-free 0.180 |
| 3BDH Crystal structure of zinc-deficient wild-type E. coli alkaline phosphatase Deposited 2007-11-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
22–471（450 aa）
Chain B
22–471（450 aa）
|
Not recorded | MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1 M MES, 0.2 M LiSO4, 1 mM ZnCl2, 5 mM MgCl2, 25-30% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.85 Å R-free 0.189 |
| 3CMR E. coli alkaline phosphatase mutant R166S in complex with phosphate Deposited 2008-03-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：R166S Mutation：R166S | ZN ZINC ION × 4 PO4 PHOSPHATE ION × 2 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.8；298 K；22% PEG 3350, 0.2 M sodium citrate, 1 mM magnesium chloride, 5 mM sodium phosphate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.05 Å R-free 0.213 |
| 3DPC Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide Deposited 2008-07-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Mutation：S102L Mutation：S102L | PO4 PHOSPHATE ION × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9.5；314 K；39%-43% saturation ammonium sulfate, 100mM Tris pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 314K
|
Resolution 2.30 Å R-free 0.271 |
| 3DYC Structure of E322Y Alkaline Phosphatase in Complex with Inorganic Phosphate Deposited 2008-07-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
26–471（446 aa）
Fragment：alkaline phosphatase
Chain B
26–471（446 aa）
Fragment：alkaline phosphatase
|
Mutation：E322Y Mutation：E322Y | ZN ZINC ION × 6 PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；0.2 M ammonium fluoride, 20% PEG 3350, and 0.5 mM zinc chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.243 |
| 3TG0 E. coli alkaline phosphatase with bound inorganic phosphate Deposited 2011-08-16 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Fragment：unp residues 23-471
Chain B
23–471（449 aa）
Fragment：unp residues 23-471
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；277 K；20% PEG 4000, 0.2 M HEPES, 1 mM ZnCl2, 0.01 mM MgCl2, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.20 Å R-free 0.169 |
| 3TG0 E. coli alkaline phosphatase with bound inorganic phosphate Deposited 2011-08-16 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
23–471（449 aa）
Fragment：unp residues 23-471
Chain D
23–471（449 aa）
Fragment：unp residues 23-471
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；277 K；20% PEG 4000, 0.2 M HEPES, 1 mM ZnCl2, 0.01 mM MgCl2, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.20 Å R-free 0.169 |
| 4KM4 E. coli alkaline phosphatase mutant S102G/R166S in complex with inorganic phosphate Deposited 2013-05-08 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
26–470（445 aa）
Chain B
26–470（445 aa）
|
Mutation：S102G, R166S Mutation：S102G, R166S | PO4 PHOSPHATE ION × 2 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291 K；Equal parts 23.5 mg/mL enzyme in 10 mM NaMOPS and 50 mM NaCl and 0.2 M NH4F, 17-21% PEG 3350, and 500 uM ZnCl2, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.80 Å R-free 0.296 |
| 4YR1 Crystal Structure of E. Coli Alkaline Phosphatase D101A/D153A in complex with inorganic phosphate Deposited 2015-03-13 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
31–471（441 aa）
Chain B
31–471（441 aa）
|
Mutation：D101A D153A Mutation：D101A D153A | ZN ZINC ION × 4 PO4 PHOSPHATE ION × 2 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；PEG 3350, Bis-Tris, ammonium sulfate, glycerol (cryo-protectant)
|
Resolution 2.24 Å R-free 0.259 |
| 5C66 E. Coli Alkaline Phosphatase in complex with tungstate Deposited 2015-06-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–471（449 aa）
Chain B
23–471（449 aa）
|
Not recorded | ZN ZINC ION × 6 WO4 TUNGSTATE(VI)ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；291 K；sodium MOPS, sodium chloride, zinc chloride, magnesium chloride, PEG3350, ammonium fluoride, sodium tungstate
|
Resolution 2.03 Å R-free 0.240 |
| 5JTL The structure of chaperone SecB in complex with unstructured proPhoA Deposited 2016-05-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain E
1–471（471 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；301 K；Ionic strength (raw mmCIF value) 150；Pressure 1
NMR sample composition
300 uM [U-100% 13C; U-100% 15N] E.coli Chaperone SecB, 300 uM [U-100% 13C; U-100% 15N] E. Coli Alkaline Phosphatase (PhoA), 150 mM potassium chloride, 50 mM sodium phosphate, 50 mM potassium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 5JTM The structure of chaperone SecB in complex with unstructured PhoA binding site a Deposited 2016-05-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain E
1–25（25 aa）
Fragment：residues 1-25
Chain F
1–25（25 aa）
Fragment：residues 1-25
Chain G
1–25（25 aa）
Fragment：residues 1-25
Chain H
1–25（25 aa）
Fragment：residues 1-25
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；301 K；Ionic strength (raw mmCIF value) 150；Pressure 1
NMR sample composition
300 uM [U-100% 13C; U-100% 15N] SecB, 300 uM [U-100% 13C; U-100% 15N] PhoA binding site a, 150 uM potassium chloride, 50 uM sodium phosphate, 50 uM potassium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 5JTN The structure of chaperone SecB in complex with unstructured proPhoA binding site c Deposited 2016-05-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain E
91–145（55 aa）
Fragment：residues 91-145
Chain F
91–145（55 aa）
Fragment：residues 91-145
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；301 K；Ionic strength (raw mmCIF value) 150；Pressure 1
NMR sample composition
300 uM [U-100% 13C; U-100% 15N] E.coli Chaperone SecB, 300 uM [U-100% 13C; U-100% 15N] E.coli Alkaline Phosphatase (PhoA) binding site c, 150 mM potassium chloride, 50 mM sodium phosphate, 50 mM potassium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 5JTO The structure of chaperone SecB in complex with unstructured proPhoA binding site d Deposited 2016-05-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain E
271–310（40 aa）
Fragment：residues 271-310
Chain F
271–310（40 aa）
Fragment：residues 271-310
Chain G
271–310（40 aa）
Fragment：residues 271-310
Chain H
271–310（40 aa）
Fragment：residues 271-310
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；301 K；Ionic strength (raw mmCIF value) 150；Pressure 1
NMR sample composition
300 uM [U-100% 13C; U-100% 15N] E.coli chaperone SecB, 300 uM [U-100% 13C; U-100% 15N] E.coli Alkaline Phosphatase (PhoA) binding site d, 150 mM potassium chloride, 50 mM sodium phosphate, 50 mM potassium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 5JTP The structure of chaperone SecB in complex with unstructured proPhoA binding site e Deposited 2016-05-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain E
450–471（22 aa）
Fragment：residues 450-471
Chain F
450–471（22 aa）
Fragment：residues 450-471
Chain G
450–471（22 aa）
Fragment：residues 450-471
Chain H
450–471（22 aa）
Fragment：residues 450-471
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；301 K；Ionic strength (raw mmCIF value) 150；Pressure 1
NMR sample composition
300 uM [U-100% 13C; U-100% 15N] E.coli Chaperone SecB, 300 uM [U-100% 13C; U-100% 15N] E. Coli Alkaline Phosphatase (PhoA) binding site e, 150 mM potassium chloride, 50 mM sodium phosphate, 50 mM potassium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 5TPQ E. coli alkaline phosphatase D101A, D153A, R166S, E322A, K328A mutant Deposited 2016-10-20 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
30–471（442 aa）
Fragment：UNP residues 30-471
Chain B
30–471（442 aa）
Fragment：UNP residues 30-471
|
Mutation：D101A, D153A, R166S, E322A, K328A Mutation：D101A, D153A, R166S, E322A, K328A | ZN ZINC ION × 7 PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；5 mg/mL protein in 10 mM MOPS pH 7.0, 50 mM NaCl, 100 mM ZnCl2. Protein solution mixed with equal volume of precipitant solution: 23% PEG 3350, 0.2 M NH3F, 0.2 M HEPES pH=8.0
|
Resolution 2.45 Å R-free 0.219 |
| 6PPT Structural Basis for Client Recognition and Activity of Hsp40 Chaperones Deposited 2019-07-08 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
4–13（10 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 120；Pressure 1
NMR sample composition
1 mM [U-13C; U-15N] PhoA-CBD1, 20 mM potassium phosphate, 75 mM potassium chloride, 0.04 % sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 6PQ2 Structural Basis for Client Recognition and Activity of Hsp40 Chaperones Deposited 2019-07-08 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
11–20（10 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 100；Pressure 1
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] L11-K20_CBD1, 20 mM potassium phosphate, 75 mM potassium chloride, 0.04 % sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 6PQE Structural Basis for Client Recognition and Activity of Hsp40 Chaperones Deposited 2019-07-09 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
235–245（11 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 100；Pressure 1
NMR sample composition
1 mM [U-13C; U-15N] PhoA_235-245_DnaJ_191-256, 20 mM potassium phosphate, 75 mM potassium chloride, 0.04 % sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 6PQM Structural Basis for Client Recognition and Activity of Hsp40 Chaperones Deposited 2019-07-09 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
415–430（16 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 100；Pressure 1
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] PhoA-Hsp40/DnaJ CBD2 fusion, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 6PRI Structural Basis for Client Recognition and Activity of Hsp40 Chaperones Deposited 2019-07-10 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
437–447（11 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 100；Pressure 1
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] F-CBD2, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 6PRJ Structural Basis for Client Recognition and Activity of Hsp40 Chaperones Deposited 2019-07-10 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
457–467（11 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 100；Pressure 1
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] G-CBD2, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 6PRQ Structural Basis for Client Recognition and Activity of Hsp40 Chaperones Deposited 2019-07-10 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
179–187（9 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 100；Pressure 1
NMR sample composition
1 mM [ILVMAT-sel-1H-13C_methyls; U-15N; U-2H] C-CBD1-CBD2, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] C-CBD1-CBD2, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 7JMM Crystal structure of the substrate-binding domain of E. coli DnaK in complex with the peptide RAKNIILLSR Deposited 2020-08-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
63–72（10 aa）
|
Mutation：P63R, I71S, G72R | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.4 M (NH4)2SO4, 0.1 M K3PO4
|
Resolution 2.56 Å R-free 0.327 |
| 7JN8 Crystal structure of the substrate-binding domain of E. coli DnaK in complex with the peptide RGNTLVIVSR Deposited 2020-08-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
381–390（10 aa）
|
Mutation：E381R, T389S, A390R | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.8 M (NH4)2SO4, 0.1 M K3PO4
|
Resolution 3.09 Å R-free 0.319 |
| 7JN9 Crystal structure of the substrate-binding domain of E. coli DnaK in complex with the peptide QEHTGSQLRIAAYGP Deposited 2020-08-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
432–446（15 aa）
|
Not recorded | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.4 M (NH4)2SO4, 0.1 M K3PO4
|
Resolution 2.40 Å R-free 0.286 |
| 7JNE Crystal structure of the substrate-binding domain of E. coli DnaK in complex with the peptide RGSQLRIASR Deposited 2020-08-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
435–444（10 aa）
|
Mutation：T435R, A443S, Y444R | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.4 M (NH4)2SO4, 0.1 M K3PO4
|
Resolution 2.54 Å R-free 0.334 |
| 7N6J Crystal structure of the substrate-binding domain of E. coli DnaK in complex with the peptide RKQSTIALALLPLLFTPRR Deposited 2021-06-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–19（19 aa）
|
Mutation：M1R, V18R, T19R | SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.4 M (NH4)2SO4, 0.1 M K3PO4 pH 7.0
|
Resolution 2.00 Å R-free 0.265 |
| 7N6K Crystal structure of the substrate-binding domain of E. coli DnaK in complex with the peptide RALALLPLSR Deposited 2021-06-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
6–15（10 aa）
|
Mutation：I6R, L14S, F15R | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；2.6 M (NH4)2SO4, 0.1 M K3PO4
|
Resolution 2.55 Å R-free 0.306 |
| 7N6L Crystal structure of the substrate-binding domain of E. coli DnaK in complex with the peptide EANQQKPLLGLFADG Deposited 2021-06-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
270–284（15 aa）
|
Not recorded | GOL GLYCEROL × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.8 M (NH4)2SO4, 0.1 M K3PO4
|
Resolution 2.40 Å R-free 0.332 |
| 7N6M Crystal structure of the substrate-binding domain of E. coli DnaK in complex with the peptide RQKPLLGLSR Deposited 2021-06-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
273–282（10 aa）
|
Mutation：Q273R, F281S, A282R | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.6 M (NH4)2SO4, 0.1 M K3PO4
|
Resolution 1.82 Å R-free 0.287 |