当前蛋白身份：P03899
重新检索
差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 6G2J Mouse mitochondrial complex I in the active state 提交 2018-03-23 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE；8-16s blotting.
|
分辨率 3.30 Å |
| 6G72 Mouse mitochondrial complex I in the deactive state 提交 2018-04-04 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE；8-16s blotting.
|
分辨率 3.90 Å |
| 6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A 提交 2020-07-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 A
1–115（115 aa）
|
突变：N-terminal Formylation 非标准单体：是（mmCIF未提供具体位点） | SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14；pH corrected at room temperature
cryo-EM玻璃化条件
冷冻剂 ETHANE；blot for 10 seconds before plunging
|
分辨率 3.10 Å |
| 6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A 提交 2020-07-20 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14；pH corrected at room temperature
cryo-EM玻璃化条件
冷冻剂 ETHANE；blot for 10 seconds before plunging
|
分辨率 3.00 Å |
| 7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A 提交 2020-09-29 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE；8-16s blotting
|
分辨率 3.17 Å |
| 7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A 提交 2020-09-29 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.82 Å |
| 7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A 提交 2020-12-14 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE；blot for 10 seconds before plunging
|
分辨率 3.04 Å |
| 7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms 提交 2021-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.40 Å |
| 8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). 提交 2022-12-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 43 PDB 声明：43-meric（43） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.90 Å |
| 8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). 提交 2023-01-24 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 43 PDB 声明：43-meric（43） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.20 Å |
| 8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). 提交 2023-01-24 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 43 PDB 声明：43-meric（43） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.90 Å |
| 8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature 提交 2023-02-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 67 PDB 声明：67-meric（67） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.20 Å |
| 8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature 提交 2023-02-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.20 Å |
| 8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature 提交 2023-02-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 66 PDB 声明：66-meric（66） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.30 Å |
| 8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature 提交 2023-02-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.30 Å |
| 8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature 提交 2023-02-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 66 PDB 声明：66-meric（66） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.30 Å |
| 8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature 提交 2023-02-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.20 Å |
| 8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature 提交 2023-02-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 67 PDB 声明：67-meric（67） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.20 Å |
| 8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature 提交 2023-02-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.30 Å |
| 8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature 提交 2023-02-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 66 PDB 声明：66-meric（66） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 6.30 Å |
| 8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature 提交 2023-02-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.20 Å |
| 8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A 提交 2023-03-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14；PH corrected at room temperature
cryo-EM玻璃化条件
冷冻剂 ETHANE；blot for 10 to 12 seconds before plunging
|
分辨率 2.84 Å |
| 8OM1 Mitochondrial complex I from Mus musculus in the active state 提交 2023-03-31 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE；blot for 10 seconds before plunging
|
分辨率 2.39 Å |
| 8PW5 CS respirasome from murine liver 提交 2023-07-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 92 PDB 声明：92-meric（92） 与蛋白数一致 |
链 A1
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.7
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.60 Å |
| 8PW6 C respirasome from murine liver 提交 2023-07-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 79 PDB 声明：79-meric（79） 与蛋白数一致 |
链 A1
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.7
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.30 Å |
| 8PW7 A respirasome from murine liver 提交 2023-07-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 79 PDB 声明：79-meric（79） 与蛋白数一致 |
链 A1
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.7
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.50 Å |
| 8RGP Closed Complex I from murine brain 提交 2023-12-14 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.7
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.00 Å |
| 8RGQ Open Complex I from murine liver 提交 2023-12-14 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.7
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.00 Å |
| 8RGR Closed Complex I from murine liver 提交 2023-12-14 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.7
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.90 Å |
| 8RGT Open Complex I from murine brain 提交 2023-12-14 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 A
1–115（115 aa）
|
非标准单体：是（mmCIF未提供具体位点） | SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.7
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.10 Å |
| 8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects 提交 2023-09-26 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 111 PDB 声明：111-meric（111） 与蛋白数一致 |
链 3
1–115（115 aa）
链 3a
1–115（115 aa）
|
未记录 | MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.7
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.70 Å |
| 8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.10 Å |
| 8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.50 Å |
| 8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.60 Å |
| 8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.40 Å |
| 8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.50 Å |
| 8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.70 Å |
| 8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.30 Å |
| 8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.50 Å |
| 8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.10 Å |
| 8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.60 Å |
| 8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.30 Å |
| 8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.60 Å |
| 8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.50 Å |
| 8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.10 Å |
| 8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.30 Å |
| 8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation 提交 2023-12-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 23 PDB 声明：23-meric（23） 与蛋白数一致 |
链 A
1–115（115 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.20 Å |