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8pw7

A respirasome from murine liver

Method: ELECTRON MICROSCOPY Dmax: 224.3 Å Quality: EXCELLENT
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Cytochrome c oxidase subunit 1

OrganismNot specified

UniProt P00397

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain n； UniProt 1–514 Not recorded Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain a 1–514（514 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain a 1–514（514 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain a 1–514（514 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain a 1–514（514 aa）
Chain n 1–514（514 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain n 1–514（514 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name COX1_MOUSE
Isoform —
PDB entities 1
Chains and sequence ranges Author chain n； PDBConstruct 1–514； UniProt 1–514

Cytochrome c oxidase subunit 2

OrganismNot specified

UniProt P00405

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain o； UniProt 1–227 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain b 1–227（227 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain b 1–227（227 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain b 1–227（227 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain b 1–227（227 aa）
Chain o 1–227（227 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain o 1–227（227 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name COX2_MOUSE
Isoform —
PDB entities 2
Chains and sequence ranges Author chain o； PDBConstruct 1–227； UniProt 1–227

Cytochrome c oxidase subunit 3

OrganismNot specified

UniProt P00416

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain p； UniProt 1–261 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain c 1–261（261 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain c 1–261（261 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain c 1–261（261 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain c 1–261（261 aa）
Chain p 1–261（261 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain p 1–261（261 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name COX3_MOUSE
Isoform —
PDB entities 3
Chains and sequence ranges Author chain p； PDBConstruct 1–261； UniProt 1–261

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

OrganismNot specified

UniProt P19783

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain q； UniProt 1–169 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain d 23–169（147 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain d 23–169（147 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain d 23–169（147 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain d 1–169（169 aa）
Chain q 1–169（169 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain q 1–169（169 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name COX41_MOUSE
Isoform —
PDB entities 4
Chains and sequence ranges Author chain q； PDBConstruct 1–169； UniProt 1–169

Cytochrome c oxidase subunit 5A, mitochondrial

OrganismNot specified

UniProt P12787

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain r； UniProt 1–146 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain e 38–146（109 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain e 38–146（109 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain e 38–146（109 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain e 1–146（146 aa）
Chain r 1–146（146 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain r 1–146（146 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name COX5A_MOUSE
Isoform —
PDB entities 5
Chains and sequence ranges Author chain r； PDBConstruct 1–146； UniProt 1–146

Cytochrome c oxidase subunit 5B, mitochondrial

OrganismNot specified

UniProt P19536

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain s； UniProt 1–128 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain f 30–128（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain f 30–128（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain f 30–128（99 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain f 1–128（128 aa）
Chain s 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain s 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name COX5B_MOUSE
Isoform —
PDB entities 6
Chains and sequence ranges Author chain s； PDBConstruct 1–128； UniProt 1–128

Cytochrome c oxidase subunit 6A1, mitochondrial

OrganismNot specified

UniProt P43024

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain t； UniProt 1–111 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain g 1–111（111 aa）
Chain t 1–111（111 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain t 1–111（111 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CX6A1_MOUSE
Isoform —
PDB entities 7
Chains and sequence ranges Author chain t； PDBConstruct 1–111； UniProt 1–111

Cytochrome c oxidase subunit 6B1

OrganismNot specified

UniProt P56391

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain u； UniProt 1–86 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain h 2–86（85 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain h 2–86（85 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain h 2–86（85 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain h 1–86（86 aa）
Chain u 1–86（86 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain u 1–86（86 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CX6B1_MOUSE
Isoform —
PDB entities 8
Chains and sequence ranges Author chain u； PDBConstruct 1–86； UniProt 1–86

Cytochrome c oxidase subunit 6C

OrganismNot specified

UniProt Q9CPQ1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain v； UniProt 1–76 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain i 2–76（75 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain i 2–76（75 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain i 2–76（75 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain i 1–76（76 aa）
Chain v 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain v 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name COX6C_MOUSE
Isoform —
PDB entities 9
Chains and sequence ranges Author chain v； PDBConstruct 1–76； UniProt 1–76

Cytochrome c oxidase subunit 7B, mitochondrial

OrganismNot specified

UniProt P56393

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain x； UniProt 1–80 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain k 25–80（56 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain k 25–80（56 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain k 25–80（56 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain k 1–80（80 aa）
Chain x 1–80（80 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain x 1–80（80 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name COX7B_MOUSE
Isoform —
PDB entities 10
Chains and sequence ranges Author chain x； PDBConstruct 1–80； UniProt 1–80

Cytochrome c oxidase subunit 7C, mitochondrial

OrganismNot specified

UniProt P17665

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain y； UniProt 1–63 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain l 17–63（47 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain l 17–63（47 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain l 17–63（47 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain l 1–63（63 aa）
Chain y 1–63（63 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain y 1–63（63 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name COX7C_MOUSE
Isoform —
PDB entities 11
Chains and sequence ranges Author chain y； PDBConstruct 1–63； UniProt 1–63

Cytochrome c oxidase subunit 7A2, mitochondrial

OrganismNot specified

UniProt P48771

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain w； UniProt 1–83 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain w 1–83（83 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain w 1–83（83 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CX7A2_MOUSE
Isoform —
PDB entities 12
Chains and sequence ranges Author chain w； PDBConstruct 1–83； UniProt 1–83

Cytochrome b-c1 complex subunit 1, mitochondrial

OrganismNot specified

UniProt Q9CZ13

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain A； UniProt 1–480 Chain L； UniProt 1–480 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain A 35–480（446 aa）
Chain L 35–480（446 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain A 35–480（446 aa）
Chain L 35–480（446 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain A 35–480（446 aa）
Chain L 35–480（446 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7O3H Murine CIII2 focus-refined from supercomplex CICIII2 Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain A 35–480（446 aa）
Chain L 35–480（446 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AA 1–480（480 aa）
Chain Aa 1–480（480 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAR Respiratory complex CIII2, focus-refined of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AA 1–480（480 aa）
Chain Aa 1–480（480 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AA 1–480（480 aa）
Chain Aa 1–480（480 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB7 Respiratory complex CIII2, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AA 1–480（480 aa）
Chain Aa 1–480（480 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 CDL CARDIOLIPIN × 4 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AA 1–480（480 aa）
Chain Aa 1–480（480 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBC Respiratory complex CIII2, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AA 1–480（480 aa）
Chain Aa 1–480（480 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AA 1–480（480 aa）
Chain Aa 1–480（480 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBG Respiratory complex CIII2, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AA 1–480（480 aa）
Chain Aa 1–480（480 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 CDL CARDIOLIPIN × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AA 1–480（480 aa）
Chain Aa 1–480（480 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC5 Respiratory complex CIII2, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AA 1–480（480 aa）
Chain Aa 1–480（480 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain A 1–480（480 aa）
Chain L 1–480（480 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain A 1–480（480 aa）
Chain L 1–480（480 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 3A 35–480（446 aa）
Chain 3L 35–480（446 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

17 other PDB entries and 17 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name QCR1_MOUSE
Isoform —
PDB entities 13
Chains and sequence ranges Author chain A； PDBConstruct 1–480； UniProt 1–480 Author chain L； PDBConstruct 1–480； UniProt 1–480

Cytochrome b-c1 complex subunit 2, mitochondrial

OrganismNot specified

UniProt Q9DB77

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain B； UniProt 1–453 Chain M； UniProt 1–453 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain B 15–453（439 aa）
Chain M 15–453（439 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain B 15–453（439 aa）
Chain M 15–453（439 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain B 15–453（439 aa）
Chain M 15–453（439 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7O3H Murine CIII2 focus-refined from supercomplex CICIII2 Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain B 15–453（439 aa）
Chain M 15–453（439 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AB 1–453（453 aa）
Chain Ab 1–453（453 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAR Respiratory complex CIII2, focus-refined of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AB 1–453（453 aa）
Chain Ab 1–453（453 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AB 1–453（453 aa）
Chain Ab 1–453（453 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB7 Respiratory complex CIII2, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AB 1–453（453 aa）
Chain Ab 1–453（453 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 CDL CARDIOLIPIN × 4 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AB 1–453（453 aa）
Chain Ab 1–453（453 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBC Respiratory complex CIII2, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AB 1–453（453 aa）
Chain Ab 1–453（453 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AB 1–453（453 aa）
Chain Ab 1–453（453 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBG Respiratory complex CIII2, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AB 1–453（453 aa）
Chain Ab 1–453（453 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 CDL CARDIOLIPIN × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AB 1–453（453 aa）
Chain Ab 1–453（453 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC5 Respiratory complex CIII2, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AB 1–453（453 aa）
Chain Ab 1–453（453 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain B 1–453（453 aa）
Chain M 1–453（453 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain B 1–453（453 aa）
Chain M 1–453（453 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 3B 15–453（439 aa）
Chain 3M 15–453（439 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

17 other PDB entries and 17 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name QCR2_MOUSE
Isoform —
PDB entities 14
Chains and sequence ranges Author chain B； PDBConstruct 1–453； UniProt 1–453 Author chain M； PDBConstruct 1–453； UniProt 1–453

Cytochrome b

OrganismNot specified

UniProt P00158

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain C； UniProt 1–381 Chain N； UniProt 1–381 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain C 1–381（381 aa）
Chain N 1–381（381 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain C 1–381（381 aa）
Chain N 1–381（381 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain C 1–381（381 aa）
Chain N 1–381（381 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7O3H Murine CIII2 focus-refined from supercomplex CICIII2 Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain C 1–381（381 aa）
Chain N 1–381（381 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AC 1–381（381 aa）
Chain Ac 1–381（381 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAR Respiratory complex CIII2, focus-refined of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AC 1–381（381 aa）
Chain Ac 1–381（381 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AC 1–381（381 aa）
Chain Ac 1–381（381 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB7 Respiratory complex CIII2, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AC 1–381（381 aa）
Chain Ac 1–381（381 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 CDL CARDIOLIPIN × 4 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AC 1–381（381 aa）
Chain Ac 1–381（381 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBC Respiratory complex CIII2, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AC 1–381（381 aa）
Chain Ac 1–381（381 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AC 1–381（381 aa）
Chain Ac 1–381（381 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBG Respiratory complex CIII2, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AC 1–381（381 aa）
Chain Ac 1–381（381 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 CDL CARDIOLIPIN × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AC 1–381（381 aa）
Chain Ac 1–381（381 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC5 Respiratory complex CIII2, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AC 1–381（381 aa）
Chain Ac 1–381（381 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain C 1–381（381 aa）
Chain N 1–381（381 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain C 1–381（381 aa）
Chain N 1–381（381 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 3C 1–380（380 aa）
Chain 3N 1–380（380 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

17 other PDB entries and 17 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CYB_MOUSE
Isoform —
PDB entities 15
Chains and sequence ranges Author chain C； PDBConstruct 1–381； UniProt 1–381 Author chain N； PDBConstruct 1–381； UniProt 1–381

Cytochrome c1, heme protein, mitochondrial

OrganismNot specified

UniProt Q9D0M3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain D； UniProt 1–325 Chain O； UniProt 1–325 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 85–325（241 aa）
Chain O 85–325（241 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 85–325（241 aa）
Chain O 85–325（241 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain D 85–325（241 aa）
Chain O 85–325（241 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7O3H Murine CIII2 focus-refined from supercomplex CICIII2 Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain D 85–325（241 aa）
Chain O 85–325（241 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AD 1–325（325 aa）
Chain Ad 1–325（325 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAR Respiratory complex CIII2, focus-refined of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AD 1–325（325 aa）
Chain Ad 1–325（325 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AD 1–325（325 aa）
Chain Ad 1–325（325 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB7 Respiratory complex CIII2, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AD 1–325（325 aa）
Chain Ad 1–325（325 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 CDL CARDIOLIPIN × 4 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AD 1–325（325 aa）
Chain Ad 1–325（325 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBC Respiratory complex CIII2, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AD 1–325（325 aa）
Chain Ad 1–325（325 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AD 1–325（325 aa）
Chain Ad 1–325（325 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBG Respiratory complex CIII2, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AD 1–325（325 aa）
Chain Ad 1–325（325 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 CDL CARDIOLIPIN × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AD 1–325（325 aa）
Chain Ad 1–325（325 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC5 Respiratory complex CIII2, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AD 1–325（325 aa）
Chain Ad 1–325（325 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain D 1–325（325 aa）
Chain O 1–325（325 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain D 1–325（325 aa）
Chain O 1–325（325 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 3D 85–325（241 aa）
Chain 3O 85–325（241 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

17 other PDB entries and 17 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CY1_MOUSE
Isoform —
PDB entities 16
Chains and sequence ranges Author chain D； PDBConstruct 1–325； UniProt 1–325 Author chain O； PDBConstruct 1–325； UniProt 1–325

Cytochrome b-c1 complex subunit Rieske, mitochondrial

OrganismNot specified

UniProt Q9CR68

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain E； UniProt 1–274 Chain P； UniProt 1–274 Chain T； UniProt 1–274 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain E 79–274（196 aa）
Chain P 79–274（196 aa）
Chain T 1–78（78 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain E 79–274（196 aa）
Chain P 79–274（196 aa）
Chain T 1–78（78 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain P 79–274（196 aa）
Chain T 1–78（78 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7O3H Murine CIII2 focus-refined from supercomplex CICIII2 Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain E 79–274（196 aa）
Chain P 79–274（196 aa）
Chain T 1–78（78 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AE 1–274（274 aa）
Chain AI 1–274（274 aa）
Chain Ae 1–274（274 aa）
Chain Ai 1–274（274 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAR Respiratory complex CIII2, focus-refined of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AE 1–274（274 aa）
Chain AI 1–274（274 aa）
Chain Ae 1–274（274 aa）
Chain Ai 1–274（274 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AE 1–274（274 aa）
Chain AI 1–274（274 aa）
Chain Ae 1–274（274 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB7 Respiratory complex CIII2, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AE 1–274（274 aa）
Chain AI 1–274（274 aa）
Chain Ae 1–274（274 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 CDL CARDIOLIPIN × 4 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AE 1–274（274 aa）
Chain AI 1–274（274 aa）
Chain Ae 1–274（274 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBC Respiratory complex CIII2, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AE 1–274（274 aa）
Chain AI 1–274（274 aa）
Chain Ae 1–274（274 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AE 1–274（274 aa）
Chain AI 1–274（274 aa）
Chain Ae 1–274（274 aa）
Chain Ai 1–274（274 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBG Respiratory complex CIII2, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AE 1–274（274 aa）
Chain AI 1–274（274 aa）
Chain Ae 1–274（274 aa）
Chain Ai 1–274（274 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 CDL CARDIOLIPIN × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AE 1–274（274 aa）
Chain AI 1–274（274 aa）
Chain Ae 1–274（274 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC5 Respiratory complex CIII2, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AE 1–274（274 aa）
Chain AI 1–274（274 aa）
Chain Ae 1–274（274 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain E 1–274（274 aa）
Chain P 1–274（274 aa）
Chain T 1–274（274 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain E 1–274（274 aa）
Chain P 1–274（274 aa）
Chain T 1–274（274 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 3E 79–274（196 aa）
Chain 3P 79–274（196 aa）
Chain 3T 1–78（78 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

17 other PDB entries and 17 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name UCRI_MOUSE
Isoform —
PDB entities 17
Chains and sequence ranges Author chain E； PDBConstruct 1–274； UniProt 1–274 Author chain P； PDBConstruct 1–274； UniProt 1–274 Author chain T； PDBConstruct 1–274； UniProt 1–274

Cytochrome b-c1 complex subunit 7

OrganismNot specified

UniProt Q9CQB4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain F； UniProt 1–111 Chain Q； UniProt 1–111 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain F 2–111（110 aa）
Chain Q 2–111（110 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain F 2–111（110 aa）
Chain Q 2–111（110 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain F 2–111（110 aa）
Chain Q 2–111（110 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7O3H Murine CIII2 focus-refined from supercomplex CICIII2 Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain F 2–111（110 aa）
Chain Q 2–111（110 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AF 1–111（111 aa）
Chain Af 1–111（111 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAR Respiratory complex CIII2, focus-refined of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AF 1–111（111 aa）
Chain Af 1–111（111 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AF 1–111（111 aa）
Chain Af 1–111（111 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB7 Respiratory complex CIII2, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AF 1–111（111 aa）
Chain Af 1–111（111 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 CDL CARDIOLIPIN × 4 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AF 1–111（111 aa）
Chain Af 1–111（111 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBC Respiratory complex CIII2, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AF 1–111（111 aa）
Chain Af 1–111（111 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AF 1–111（111 aa）
Chain Af 1–111（111 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBG Respiratory complex CIII2, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AF 1–111（111 aa）
Chain Af 1–111（111 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 CDL CARDIOLIPIN × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AF 1–111（111 aa）
Chain Af 1–111（111 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC5 Respiratory complex CIII2, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AF 1–111（111 aa）
Chain Af 1–111（111 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain F 1–111（111 aa）
Chain Q 1–111（111 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain F 1–111（111 aa）
Chain Q 1–111（111 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 3F 2–111（110 aa）
Chain 3Q 2–111（110 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

17 other PDB entries and 17 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q9CQB4_MOUSE
Isoform —
PDB entities 18
Chains and sequence ranges Author chain F； PDBConstruct 1–111； UniProt 1–111 Author chain Q； PDBConstruct 1–111； UniProt 1–111

Cytochrome b-c1 complex subunit 8

OrganismNot specified

UniProt Q9CQ69

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain G； UniProt 1–82 Chain R； UniProt 1–82 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain G 2–82（81 aa）
Chain R 2–82（81 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain G 2–82（81 aa）
Chain R 2–82（81 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain G 2–82（81 aa）
Chain R 2–82（81 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7O3H Murine CIII2 focus-refined from supercomplex CICIII2 Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain G 2–82（81 aa）
Chain R 2–82（81 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AG 1–82（82 aa）
Chain Ag 1–82（82 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAR Respiratory complex CIII2, focus-refined of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AG 1–82（82 aa）
Chain Ag 1–82（82 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AG 1–82（82 aa）
Chain Ag 1–82（82 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB7 Respiratory complex CIII2, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AG 1–82（82 aa）
Chain Ag 1–82（82 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 CDL CARDIOLIPIN × 4 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AG 1–82（82 aa）
Chain Ag 1–82（82 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBC Respiratory complex CIII2, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AG 1–82（82 aa）
Chain Ag 1–82（82 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AG 1–82（82 aa）
Chain Ag 1–82（82 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBG Respiratory complex CIII2, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AG 1–82（82 aa）
Chain Ag 1–82（82 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 CDL CARDIOLIPIN × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AG 1–82（82 aa）
Chain Ag 1–82（82 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC5 Respiratory complex CIII2, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AG 1–82（82 aa）
Chain Ag 1–82（82 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain G 1–82（82 aa）
Chain R 1–82（82 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain G 1–82（82 aa）
Chain R 1–82（82 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 3G 2–82（81 aa）
Chain 3R 2–82（81 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

17 other PDB entries and 17 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name QCR8_MOUSE
Isoform —
PDB entities 19
Chains and sequence ranges Author chain G； PDBConstruct 1–82； UniProt 1–82 Author chain R； PDBConstruct 1–82； UniProt 1–82

Cytochrome b-c1 complex subunit 6, mitochondrial

OrganismNot specified

UniProt P99028

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain H； UniProt 1–89 Chain S； UniProt 1–89 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain H 14–89（76 aa）
Chain S 14–89（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain H 14–89（76 aa）
Chain S 14–89（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain H 14–89（76 aa）
Chain S 14–89（76 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7O3H Murine CIII2 focus-refined from supercomplex CICIII2 Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain H 14–89（76 aa）
Chain S 14–89（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AH 1–89（89 aa）
Chain Ah 1–89（89 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAR Respiratory complex CIII2, focus-refined of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AH 1–89（89 aa）
Chain Ah 1–89（89 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AH 1–89（89 aa）
Chain Ah 1–89（89 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB7 Respiratory complex CIII2, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AH 1–89（89 aa）
Chain Ah 1–89（89 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 CDL CARDIOLIPIN × 4 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AH 1–89（89 aa）
Chain Ah 1–89（89 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBC Respiratory complex CIII2, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AH 1–89（89 aa）
Chain Ah 1–89（89 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AH 1–89（89 aa）
Chain Ah 1–89（89 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBG Respiratory complex CIII2, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AH 1–89（89 aa）
Chain Ah 1–89（89 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 CDL CARDIOLIPIN × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AH 1–89（89 aa）
Chain Ah 1–89（89 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC5 Respiratory complex CIII2, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AH 1–89（89 aa）
Chain Ah 1–89（89 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain H 1–89（89 aa）
Chain S 1–89（89 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain H 1–89（89 aa）
Chain S 1–89（89 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 3H 14–89（76 aa）
Chain 3S 14–89（76 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

17 other PDB entries and 17 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name QCR6_MOUSE
Isoform —
PDB entities 20
Chains and sequence ranges Author chain H； PDBConstruct 1–89； UniProt 1–89 Author chain S； PDBConstruct 1–89； UniProt 1–89

Cytochrome b-c1 complex subunit 9

OrganismNot specified

UniProt Q8R1I1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain J； UniProt 1–64 Chain U； UniProt 1–64 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain J 2–64（63 aa）
Chain U 2–64（63 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain J 2–64（63 aa）
Chain U 2–64（63 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3E Murine supercomplex CIII2CIV in the intermediate locked conformation Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 31 PDB declaration: 31-meric
Chain J 2–64（63 aa）
Chain U 2–64（63 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 5 HEC HEME C × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7O3H Murine CIII2 focus-refined from supercomplex CICIII2 Deposited 2021-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain J 2–64（63 aa）
Chain U 2–64（63 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AJ 1–64（64 aa）
Chain Aj 1–64（64 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAR Respiratory complex CIII2, focus-refined of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AJ 1–64（64 aa）
Chain Aj 1–64（64 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AJ 1–64（64 aa）
Chain Aj 1–64（64 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB7 Respiratory complex CIII2, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AJ 1–64（64 aa）
Chain Aj 1–64（64 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 CDL CARDIOLIPIN × 4 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AJ 1–64（64 aa）
Chain Aj 1–64（64 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBC Respiratory complex CIII2, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AJ 1–64（64 aa）
Chain Aj 1–64（64 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AJ 1–64（64 aa）
Chain Aj 1–64（64 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBG Respiratory complex CIII2, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AJ 1–64（64 aa）
Chain Aj 1–64（64 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 CDL CARDIOLIPIN × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AJ 1–64（64 aa）
Chain Aj 1–64（64 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC5 Respiratory complex CIII2, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AJ 1–64（64 aa）
Chain Aj 1–64（64 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain J 1–64（64 aa）
Chain U 1–64（64 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain J 1–64（64 aa）
Chain U 1–64（64 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 3J 2–64（63 aa）
Chain 3U 2–64（63 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

17 other PDB entries and 17 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name QCR9_MOUSE
Isoform —
PDB entities 21
Chains and sequence ranges Author chain J； PDBConstruct 1–64； UniProt 1–64 Author chain U； PDBConstruct 1–64； UniProt 1–64

Cytochrome b-c1 complex subunit 10

OrganismNot specified

UniProt Q9CPX8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain K； UniProt 1–56 Chain V； UniProt 1–56 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7O37 Murine supercomplex CIII2CIV in the assembled locked conformation Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain K 1–56（56 aa）
Chain V 1–56（56 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 TGL TRISTEAROYLGLYCEROL × 1 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O3C Murine supercomplex CIII2CIV in the mature unlocked conformation Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain K 1–56（56 aa）
Chain V 1–56（56 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 15 CDL CARDIOLIPIN × 7 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 1 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 1 TGL TRISTEAROYLGLYCEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7O3H Murine CIII2 focus-refined from supercomplex CICIII2 Deposited 2021-04-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain K 1–56（56 aa）
Chain V 1–56（56 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7；CHAPS was added only upon freezing
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AK 1–56（56 aa）
Chain Ak 1–56（56 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAR Respiratory complex CIII2, focus-refined of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AK 1–56（56 aa）
Chain Ak 1–56（56 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AK 1–56（56 aa）
Chain Ak 1–56（56 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB7 Respiratory complex CIII2, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AK 1–56（56 aa）
Chain Ak 1–56（56 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 CDL CARDIOLIPIN × 4 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AK 1–56（56 aa）
Chain Ak 1–56（56 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBC Respiratory complex CIII2, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AK 1–56（56 aa）
Chain Ak 1–56（56 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain AK 1–56（56 aa）
Chain Ak 1–56（56 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBG Respiratory complex CIII2, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain AK 1–56（56 aa）
Chain Ak 1–56（56 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 CDL CARDIOLIPIN × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain AK 1–56（56 aa）
Chain Ak 1–56（56 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC5 Respiratory complex CIII2, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain AK 1–56（56 aa）
Chain Ak 1–56（56 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain K 1–56（56 aa）
Chain V 1–56（56 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain K 1–56（56 aa）
Chain V 1–56（56 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 3K 1–56（56 aa）
Chain 3V 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

16 other PDB entries and 16 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name QCR10_MOUSE
Isoform —
PDB entities 22
Chains and sequence ranges Author chain K； PDBConstruct 1–56； UniProt 1–56 Author chain V； PDBConstruct 1–56； UniProt 1–56

NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

OrganismNot specified

UniProt Q9DC70

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain 6； UniProt 1–224 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–224（224 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–224（224 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain 6 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain 6 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 6 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 6 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 6 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 6 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain S7 1–224（224 aa）
Chain s7 1–224（224 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain B 1–224（224 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

46 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS7_MOUSE
Isoform —
PDB entities 23
Chains and sequence ranges Author chain 6； PDBConstruct 1–224； UniProt 1–224

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

OrganismNot specified

UniProt Q9DCT2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain C1； UniProt 1–263 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–263（263 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–263（263 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain C1 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain C1 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain S3 1–263（263 aa）
Chain s3 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain C 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

46 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS3_MOUSE
Isoform —
PDB entities 24
Chains and sequence ranges Author chain C1； PDBConstruct 1–263； UniProt 1–263

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

OrganismNot specified

UniProt Q91WD5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain D1； UniProt 1–463 Non-standard monomer：Yes (specific site not provided by mmCIF) Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain D 1–463（463 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain D 1–463（463 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain D1 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain D1 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain S2 1–463（463 aa）
Chain s2 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain D 1–463（463 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

51 other PDB entries and 51 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS2_MOUSE
Isoform —
PDB entities 25
Chains and sequence ranges Author chain D1； PDBConstruct 1–463； UniProt 1–463

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

OrganismNot specified

UniProt Q9D6J6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain 2； UniProt 1–248 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–245（245 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain E 1–245（245 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain E 1–245（245 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA4 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2 N-domain). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain E 1–248（248 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 2 SF4 IRON/SULFUR CLUSTER × 3 FMN FLAVIN MONONUCLEOTIDE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.25 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–248（248 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–248（248 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain 2 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain 2 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 2 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 2 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 2 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 2 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain V2 1–244（244 aa）
Chain v2 1–244（244 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain E 1–248（248 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

47 other PDB entries and 47 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUV2_MOUSE
Isoform —
PDB entities 26
Chains and sequence ranges Author chain 2； PDBConstruct 1–248； UniProt 1–248

NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

OrganismNot specified

UniProt Q91YT0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain 1； UniProt 1–464 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 30–457（428 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA4 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2 N-domain). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain F 1–464（464 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 2 SF4 IRON/SULFUR CLUSTER × 3 FMN FLAVIN MONONUCLEOTIDE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.25 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain 1 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain 1 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 1 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 1 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 1 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 1 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain V1 1–457（457 aa）
Chain v1 1–457（457 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

47 other PDB entries and 47 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUV1_MOUSE
Isoform —
PDB entities 27
Chains and sequence ranges Author chain 1； PDBConstruct 1–464； UniProt 1–464

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

OrganismNot specified

UniProt Q91VD9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain 3； UniProt 1–727 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–715（715 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA4 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2 N-domain). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain G 1–727（727 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 2 SF4 IRON/SULFUR CLUSTER × 3 FMN FLAVIN MONONUCLEOTIDE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.25 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain 3 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain 3 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 3 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 3 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 3 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 3 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain S1 1–716（716 aa）
Chain s1 1–716（716 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain G 1–727（727 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

47 other PDB entries and 47 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS1_MOUSE
Isoform —
PDB entities 28
Chains and sequence ranges Author chain 3； PDBConstruct 1–727； UniProt 1–727

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

OrganismNot specified

UniProt Q8K3J1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain 9； UniProt 1–212 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain 9 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain 9 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 9 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 9 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 9 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 9 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain S8 1–212（212 aa）
Chain s8 1–212（212 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain I 1–212（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

46 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS8_MOUSE
Isoform —
PDB entities 29
Chains and sequence ranges Author chain 9； PDBConstruct 1–212； UniProt 1–212

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial

OrganismNot specified

UniProt Q9DC69

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain P1； UniProt 1–377 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–377（377 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–377（377 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain P1 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain P1 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain A9 1–377（377 aa）
Chain a9 1–377（377 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain P 1–377（377 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

46 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA9_MOUSE
Isoform —
PDB entities 30
Chains and sequence ranges Author chain P1； PDBConstruct 1–377； UniProt 1–377

NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

OrganismNot specified

UniProt Q9CXZ1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain Q1； UniProt 1–175 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain Q1 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain Q1 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain S4 1–175（175 aa）
Chain s4 1–175（175 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Q 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

43 other PDB entries and 43 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS4_MOUSE
Isoform —
PDB entities 31
Chains and sequence ranges Author chain Q1； PDBConstruct 1–175； UniProt 1–175

NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

OrganismNot specified

UniProt P52503

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain 7； UniProt 1–116 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–114（114 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain 7 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain 7 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 7 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 7 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 7 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 7 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain S6 1–116（116 aa）
Chain s6 1–116（116 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain R 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

45 other PDB entries and 45 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS6_MOUSE
Isoform —
PDB entities 32
Chains and sequence ranges Author chain 7； PDBConstruct 1–116； UniProt 1–116

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

OrganismNot specified

UniProt Q9CQ75

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain S1； UniProt 1–99 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA4 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2 N-domain). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain S 1–99（99 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 2 SF4 IRON/SULFUR CLUSTER × 3 FMN FLAVIN MONONUCLEOTIDE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.25 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain S1 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain S1 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain A2 1–99（99 aa）
Chain a2 1–99（99 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

47 other PDB entries and 47 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA2_MOUSE
Isoform —
PDB entities 33
Chains and sequence ranges Author chain S1； PDBConstruct 1–99； UniProt 1–99

Acyl carrier protein, mitochondrial

OrganismNot specified

UniProt Q9CR21

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain T1； UniProt 1–156 Chain U1； UniProt 1–156 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain T1 1–156（156 aa）
Chain U1 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain T1 1–156（156 aa）
Chain U1 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain AB 1–156（156 aa）
Chain AC 1–156（156 aa）
Chain ab 1–156（156 aa）
Chain ac 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
9E9C Mouse mitoribosome large subunit assembly intermediate bound to NSUN4, METRF4, GTPBP7, GTPBP10 and the MALSU1-L0R8F8-mt-ACP complex (without uL16m), State B1 (SAMC knock-out) Deposited 2024-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 58-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 95 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.62 Å
9HCC Mouse mitoribosome large subunit assembly intermediate (without uL16m) bound to MRM3-dimer, DDX28 and the MALSU-L0R8F8-mt-ACP complex, State A1 (SAMC knock-out) Deposited 2024-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 49 PDB declaration: 51-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.67 Å
9HCD Mouse mitoribosome large subunit assembly intermediate (with uL16m) bound to MRM3-dimer, DDX28 and the MALSU-L0R8F8-mt-ACP complex, state A2 (SAMC knock-out) Deposited 2024-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 50 PDB declaration: 52-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.63 Å
9HCE Mouse mitoribosome large subunit assembly intermediate (without uL16m) bound to MRM3 dimer and the MALSU-L0R8F8-mt-ACP complex, State A3 (SAMC knock out) Deposited 2024-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 49 PDB declaration: 51-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.31 Å
9HCF Mouse mitoribosome large subunit assembly intermediate bound to NSUN4, METRF4, GTPBP7, GTPBP10 and the MALSU-L0R8F8-mtACP complex with uL16m, State B2 (SAMC knock-out) Deposited 2024-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 57 PDB declaration: 59-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 99 FES FE2/S2 (INORGANIC) CLUSTER × 1 ZN ZINC ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.85 Å
9HCG Mouse mitoribosome large subunit assembly intermediate bound to NSUN4, METRF4, MRM2, GTPBP7 and MALSU1-L0R8F8-mt-ACP complex, State D (SAMC knock-out) Deposited 2024-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 57 PDB declaration: 59-meric
Chain w 74–152（79 aa）
Not recorded MG MAGNESIUM ION × 105 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.91 Å
9HCH Mouse mitoribosome large subunit assembly intermediate bound to NSUN4, METRF4, GTPBP7 and the MALSU1-L0R8F8-mt-ACP complex, State C1 (SAMC knock-out) Deposited 2024-11-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 56 PDB declaration: 58-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 99 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.85 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 72–156（85 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9W2V Cryo-EM structure of complex I on the bovine heart submitochondrial particles, closed Deposited 2025-07-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 72–156（85 aa）
Not recorded FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å

60 other PDB entries and 60 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ACPM_MOUSE
Isoform —
PDB entities 34
Chains and sequence ranges Author chain T1； PDBConstruct 1–156； UniProt 1–156 Author chain U1； PDBConstruct 1–156； UniProt 1–156

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

OrganismNot specified

UniProt Q9CPP6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain V1； UniProt 1–116 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain V1 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain V1 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain A5 1–116（116 aa）
Chain a5 1–116（116 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

46 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA5_MOUSE
Isoform —
PDB entities 35
Chains and sequence ranges Author chain V1； PDBConstruct 1–116； UniProt 1–116

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

OrganismNot specified

UniProt Q9CQZ5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain W1； UniProt 1–131 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–131（131 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–131（131 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain W1 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain W1 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain A6 1–131（131 aa）
Chain a6 1–131（131 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain W 1–131（131 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

46 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA6_MOUSE
Isoform —
PDB entities 36
Chains and sequence ranges Author chain W1； PDBConstruct 1–131； UniProt 1–131

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

OrganismNot specified

UniProt Q7TMF3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain q1； UniProt 1–145 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain q1 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain q1 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain AN 1–145（145 aa）
Chain an 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

43 other PDB entries and 43 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUAC_MOUSE
Isoform —
PDB entities 37
Chains and sequence ranges Author chain q1； PDBConstruct 1–145； UniProt 1–145

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

OrganismNot specified

UniProt Q9Z1P6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain r1； UniProt 1–113 Non-standard monomer：Yes (specific site not provided by mmCIF) Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 2–113（112 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 2–113（112 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain r 1–113（113 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain r 1–113（113 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain r 1–113（113 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain r 1–113（113 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain r 1–113（113 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 2–113（112 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain r1 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain r1 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain A7 2–113（112 aa）
Chain a7 2–113（112 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain r 1–113（113 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

46 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA7_MOUSE
Isoform —
PDB entities 38
Chains and sequence ranges Author chain r1； PDBConstruct 1–113； UniProt 1–113

NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

OrganismNot specified

UniProt Q8BK30

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain s1； UniProt 1–104 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA4 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2 N-domain). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain s 1–104（104 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 2 SF4 IRON/SULFUR CLUSTER × 3 FMN FLAVIN MONONUCLEOTIDE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.25 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–104（104 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–104（104 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain s1 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain s1 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain V3 1–104（104 aa）
Chain v3 1–104（104 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain s 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

47 other PDB entries and 47 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUV3_MOUSE
Isoform —
PDB entities 39
Chains and sequence ranges Author chain s1； PDBConstruct 1–104； UniProt 1–104

NADH-ubiquinone oxidoreductase chain 3

OrganismNot specified

UniProt P03899

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain A1； UniProt 1–115 Non-standard monomer：Yes (specific site not provided by mmCIF) Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 1–115（115 aa）
Mutation：N-terminal Formylation Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain A 1–115（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain A 1–115（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain A1 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain A1 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 3 1–115（115 aa）
Chain 3a 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain A 1–115（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

46 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU3M_MOUSE
Isoform —
PDB entities 40
Chains and sequence ranges Author chain A1； PDBConstruct 1–115； UniProt 1–115

NADH-ubiquinone oxidoreductase chain 1

OrganismNot specified

UniProt P03888

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain H1； UniProt 1–318 Non-standard monomer：Yes (specific site not provided by mmCIF) Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7LFI MODEL OF MHC CLASS Ib H2-M3 WITH MOUSE ND1 N-TERMINAL HEPTAPEPTIDE REFINED AT 1.70 ANGSTROMS RESOLUTION Deposited 2021-01-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–7（7 aa） Fragment：First seven amino-terminal residues
Non-standard monomer：Yes (specific site not provided by mmCIF) NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；283 K；0.1 M Hepes, 20% (w/v) PEG 4000, 30% (v/v) ethylene glycol
Resolution 1.70 Å R-free 0.259
7LFI MODEL OF MHC CLASS Ib H2-M3 WITH MOUSE ND1 N-TERMINAL HEPTAPEPTIDE REFINED AT 1.70 ANGSTROMS RESOLUTION Deposited 2021-01-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain F 1–7（7 aa） Fragment：First seven amino-terminal residues
Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；283 K；0.1 M Hepes, 20% (w/v) PEG 4000, 30% (v/v) ethylene glycol
Resolution 1.70 Å R-free 0.259
7LFJ MODEL OF MHC CLASS Ib H2-M3 WITH MOUSE ND1 N-TERMINAL HEPTAPEPTIDE, ALA MUTANT, REFINED AT 1.70 ANGSTROMS RESOLUTION Deposited 2021-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–7（7 aa） Fragment：First seven amino-terminal residues
Mutation：I6A Non-standard monomer：Yes (specific site not provided by mmCIF) NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；283 K；0.1 M Hepes, 20% (w/v) PEG 4000, 30% (v/v) ethylene glycol
Resolution 1.70 Å R-free 0.207
7LFJ MODEL OF MHC CLASS Ib H2-M3 WITH MOUSE ND1 N-TERMINAL HEPTAPEPTIDE, ALA MUTANT, REFINED AT 1.70 ANGSTROMS RESOLUTION Deposited 2021-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain F 1–7（7 aa） Fragment：First seven amino-terminal residues
Mutation：I6A Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；283 K；0.1 M Hepes, 20% (w/v) PEG 4000, 30% (v/v) ethylene glycol
Resolution 1.70 Å R-free 0.207
7LFK MODEL OF MHC CLASS Ib H2-M3 WITH MOUSE ND1 N-TERMINAL HEPTAPEPTIDE, THR MUTANT, REFINED AT 1.60 ANGSTROMS RESOLUTION Deposited 2021-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–7（7 aa） Fragment：First seven amino-terminal residues
Mutation：I6T Non-standard monomer：Yes (specific site not provided by mmCIF) NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 NA SODIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；283 K；0.1 M Hepes, 20% (w/v) PEG 4000, 30% (v/v) ethylene glycol
Resolution 1.60 Å R-free 0.244
7LFK MODEL OF MHC CLASS Ib H2-M3 WITH MOUSE ND1 N-TERMINAL HEPTAPEPTIDE, THR MUTANT, REFINED AT 1.60 ANGSTROMS RESOLUTION Deposited 2021-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain F 1–7（7 aa） Fragment：First seven amino-terminal residues
Mutation：I6T Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；283 K；0.1 M Hepes, 20% (w/v) PEG 4000, 30% (v/v) ethylene glycol
Resolution 1.60 Å R-free 0.244
7LFL MODEL OF MHC CLASS Ib H2-M3 WITH MOUSE ND1 N-TERMINAL HEPTAPEPTIDE, VAL MUTANT, MONOCLINIC CELL, REFINED AT 1.60 ANGSTROMS RESOLUTION Deposited 2021-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–7（7 aa） Fragment：First seven amino-terminal residues
Mutation：I6V Non-standard monomer：Yes (specific site not provided by mmCIF) NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；283 K；0.1 M Hepes, 20% (w/v) PEG 4000, 30% (v/v) ethylene glycol
Resolution 1.60 Å R-free 0.239
7LFM MODEL OF MHC CLASS Ib H2-M3 WITH MOUSE ND1 N-TERMINAL HEPTAPEPTIDE, VAL MUTANT, TRICLINIC CELL, REFINED AT 1.60 ANGSTROMS RESOLUTION Deposited 2021-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–7（7 aa） Fragment：First seven amino-terminal residues
Mutation：I6V Non-standard monomer：Yes (specific site not provided by mmCIF) NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；283 K；0.1 M Hepes, 20% (w/v) PEG 4000, 30% (v/v) ethylene glycol
Resolution 1.60 Å R-free 0.217
7LFM MODEL OF MHC CLASS Ib H2-M3 WITH MOUSE ND1 N-TERMINAL HEPTAPEPTIDE, VAL MUTANT, TRICLINIC CELL, REFINED AT 1.60 ANGSTROMS RESOLUTION Deposited 2021-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain F 1–7（7 aa） Fragment：First seven amino-terminal residues
Mutation：I6V Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；283 K；0.1 M Hepes, 20% (w/v) PEG 4000, 30% (v/v) ethylene glycol
Resolution 1.60 Å R-free 0.217
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain H 1–318（318 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain H 1–318（318 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain H1 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain H1 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–318（318 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain H 1–318（318 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

50 other PDB entries and 54 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU1M_MOUSE
Isoform —
PDB entities 41
Chains and sequence ranges Author chain H1； PDBConstruct 1–318； UniProt 1–318

NADH-ubiquinone oxidoreductase chain 6

OrganismNot specified

UniProt P03925

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain J1； UniProt 1–172 Non-standard monomer：Yes (specific site not provided by mmCIF) Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain J 1–171（171 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain J 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain J 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain J 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain J 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain J 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain J 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain J 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain J 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain J 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain J 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain J1 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain J1 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–172（172 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 6 1–172（172 aa）
Chain 6a 1–172（172 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

29 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU6M_MOUSE
Isoform —
PDB entities 42
Chains and sequence ranges Author chain J1； PDBConstruct 1–172； UniProt 1–172

NADH-ubiquinone oxidoreductase chain 4L

OrganismNot specified

UniProt P03903

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain K1； UniProt 1–98 Non-standard monomer：Yes (specific site not provided by mmCIF) Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain K 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain K 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain K 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain K 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain K 1–98（98 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain K 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain K 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain K 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain K 1–98（98 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain K 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain K1 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain K1 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–98（98 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 4L 1–98（98 aa）
Chain 4l 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU4LM_MOUSE
Isoform —
PDB entities 43
Chains and sequence ranges Author chain K1； PDBConstruct 1–98； UniProt 1–98

NADH-ubiquinone oxidoreductase chain 5

OrganismNot specified

UniProt P03921

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain L1； UniProt 1–607 Non-standard monomer：Yes (specific site not provided by mmCIF) Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain L 1–607（607 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain L 1–607（607 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain L 1–607（607 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain L 1–607（607 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain L 1–607（607 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain L 1–607（607 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain L 1–607（607 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain L 1–607（607 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain L 1–607（607 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain L 1–607（607 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain L1 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain L1 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–607（607 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 5 1–607（607 aa）
Chain 5a 1–607（607 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU5M_MOUSE
Isoform —
PDB entities 44
Chains and sequence ranges Author chain L1； PDBConstruct 1–607； UniProt 1–607

NADH-ubiquinone oxidoreductase chain 4

OrganismNot specified

UniProt P03911

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain M1； UniProt 1–459 Non-standard monomer：Yes (specific site not provided by mmCIF) Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain M 1–459（459 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain M 1–459（459 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain M 1–459（459 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain M 1–459（459 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain M 1–459（459 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain M 1–459（459 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain M 1–459（459 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain M 1–459（459 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain M 1–459（459 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain M 1–459（459 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain M1 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain M1 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 4 1–459（459 aa）
Chain 4a 1–459（459 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU4M_MOUSE
Isoform —
PDB entities 45
Chains and sequence ranges Author chain M1； PDBConstruct 1–459； UniProt 1–459

NADH-ubiquinone oxidoreductase chain 2

OrganismNot specified

UniProt P03893

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain N1； UniProt 1–345 Non-standard monomer：Yes (specific site not provided by mmCIF) Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain N 1–345（345 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain N 1–345（345 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain N 1–345（345 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain N 1–345（345 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain N 1–345（345 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain N 1–345（345 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain N 1–345（345 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain N 1–345（345 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain N 1–345（345 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain N 1–345（345 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain N1 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain N1 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–345（345 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain 2 1–345（345 aa）
Chain 2a 1–345（345 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU2M_MOUSE
Isoform —
PDB entities 46
Chains and sequence ranges Author chain N1； PDBConstruct 1–345； UniProt 1–345

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

OrganismNot specified

UniProt Q99LC3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain O1； UniProt 1–355 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–355（355 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain O 1–355（355 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain O 1–355（355 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain O 1–355（355 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain O 1–355（355 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain O 1–355（355 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain O 1–355（355 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain O 1–355（355 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain O 1–355（355 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–355（355 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–355（355 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain O1 1–355（355 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain O1 1–355（355 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–355（355 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain AL 1–355（355 aa）
Chain al 1–355（355 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUAA_MOUSE
Isoform —
PDB entities 47
Chains and sequence ranges Author chain O1； PDBConstruct 1–355； UniProt 1–355

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

OrganismNot specified

UniProt Q9DCJ5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain X1； UniProt 1–172 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain X1 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain X1 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain A8 1–172（172 aa）
Chain a8 1–172（172 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain X 1–172（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

51 other PDB entries and 51 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA8_MOUSE
Isoform —
PDB entities 48
Chains and sequence ranges Author chain X1； PDBConstruct 1–172； UniProt 1–172

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

OrganismNot specified

UniProt G5E814

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain Y1； UniProt 3–143 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–143（143 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–143（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–143（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–143（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–143（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–143（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–143（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain Y 1–143（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain Y 1–143（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain Y 1–143（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain Y 1–143（143 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain Y 1–143（143 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain Y 1–143（143 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain Y 1–143（143 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain Y 1–143（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain Y 1–143（143 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain Y 1–143（143 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain Y 1–143（143 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain Y 1–143（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain Y 1–143（143 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–143（143 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 2–143（142 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain Y1 3–143（141 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain Y1 3–143（141 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 3–143（141 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 3–143（141 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 3–143（141 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 3–143（141 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain AM 2–143（142 aa）
Chain am 2–143（142 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

29 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G5E814_MOUSE
Isoform —
PDB entities 49
Chains and sequence ranges Author chain Y1； PDBConstruct 1–141； UniProt 3–143

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

OrganismNot specified

UniProt Q9ERS2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain Z1； UniProt 1–144 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain Z1 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain Z1 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain AO 1–144（144 aa）
Chain ao 1–144（144 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain Z 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

46 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUAD_MOUSE
Isoform —
PDB entities 50
Chains and sequence ranges Author chain Z1； PDBConstruct 1–144； UniProt 1–144

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

OrganismNot specified

UniProt O35683

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain a1； UniProt 1–70 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–67（67 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–68（68 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–68（68 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain a1 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain a1 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain A1 1–70（70 aa）
Chain a1 1–70（70 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

46 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA1_MOUSE
Isoform —
PDB entities 51
Chains and sequence ranges Author chain a1； PDBConstruct 1–70； UniProt 1–70

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

OrganismNot specified

UniProt Q9CQ91

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain b1； UniProt 1–84 Non-standard monomer：Yes (specific site not provided by mmCIF) Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 2–84（83 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain b1 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain b1 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain A3 1–84（84 aa）
Chain a3 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

46 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA3_MOUSE
Isoform —
PDB entities 52
Chains and sequence ranges Author chain b1； PDBConstruct 1–84； UniProt 1–84

NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

OrganismNot specified

UniProt Q9CQY9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain c1； UniProt 1–76 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain c1 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain c1 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain C1 1–76（76 aa）
Chain c1 1–76（76 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUC1_MOUSE
Isoform —
PDB entities 53
Chains and sequence ranges Author chain c1； PDBConstruct 1–76； UniProt 1–76

NADH dehydrogenase [ubiquinone] 1 subunit C2

OrganismNot specified

UniProt Q9CQ54

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain d1； UniProt 1–120 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain d 1–120（120 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain d 1–120（120 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain d 1–120（120 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain d 1–120（120 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain d 1–120（120 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain d 1–120（120 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain d 1–120（120 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain d 1–120（120 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain d1 1–120（120 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain d1 1–120（120 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain C2 1–120（120 aa）
Chain c2 1–120（120 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUC2_MOUSE
Isoform —
PDB entities 54
Chains and sequence ranges Author chain d1； PDBConstruct 1–120； UniProt 1–120

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

OrganismNot specified

UniProt Q99LY9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain e1； UniProt 1–106 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain e1 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain e1 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain S5 1–106（106 aa）
Chain s5 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS5_MOUSE
Isoform —
PDB entities 55
Chains and sequence ranges Author chain e1； PDBConstruct 1–106； UniProt 1–106

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

OrganismNot specified

UniProt P0DN34

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain f1； UniProt 1–57 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain f1 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain f1 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain B1 1–57（57 aa）
Chain b1 1–57（57 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB1_MOUSE
Isoform —
PDB entities 56
Chains and sequence ranges Author chain f1； PDBConstruct 1–57； UniProt 1–57

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial

OrganismNot specified

UniProt O09111

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain g1； UniProt 1–151 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–151（151 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain g 1–151（151 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain g 1–151（151 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain g 1–151（151 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain g 1–151（151 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain g 1–151（151 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain g 1–151（151 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain g 1–151（151 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain g 1–151（151 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–151（151 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–151（151 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain g1 1–151（151 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain g1 1–151（151 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain BM 1–151（151 aa）
Chain bm 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUBB_MOUSE
Isoform —
PDB entities 57
Chains and sequence ranges Author chain g1； PDBConstruct 1–151； UniProt 1–151

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial

OrganismNot specified

UniProt Q9CQH3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain h1； UniProt 1–189 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain h1 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain h1 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain B5 1–189（189 aa）
Chain b5 1–189（189 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB5_MOUSE
Isoform —
PDB entities 58
Chains and sequence ranges Author chain h1； PDBConstruct 1–189； UniProt 1–189

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

OrganismNot specified

UniProt Q3UIU2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain i1； UniProt 1–128 Non-standard monomer：Yes (specific site not provided by mmCIF) Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain i 1–128（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain i 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain i 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain i 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain i 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain i 1–128（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain i 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain i 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain i 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain i 1–128（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain i 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain i1 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain i1 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain B6 2–128（127 aa）
Chain b6 2–128（127 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB6_MOUSE
Isoform —
PDB entities 59
Chains and sequence ranges Author chain i1； PDBConstruct 1–128； UniProt 1–128

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial

OrganismNot specified

UniProt Q9CPU2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain j1； UniProt 1–105 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–105（105 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain j 1–105（105 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain j 1–105（105 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain j 1–105（105 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain j 1–105（105 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain j 1–105（105 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain j 1–105（105 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain j 1–105（105 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain j 1–105（105 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–105（105 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–105（105 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain j1 1–105（105 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain j1 1–105（105 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain B2 1–105（105 aa）
Chain b2 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB2_MOUSE
Isoform —
PDB entities 60
Chains and sequence ranges Author chain j1； PDBConstruct 1–105； UniProt 1–105

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

OrganismNot specified

UniProt Q9CQZ6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain k1； UniProt 1–104 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain k 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain k 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain k 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain k 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain k 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain k 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain k 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain k 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–104（104 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–104（104 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain k1 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain k1 1–104（104 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–104（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain B3 1–104（104 aa）
Chain b3 1–104（104 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB3_MOUSE
Isoform —
PDB entities 61
Chains and sequence ranges Author chain k1； PDBConstruct 1–104； UniProt 1–104

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

OrganismNot specified

UniProt Q9D6J5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain l1； UniProt 1–186 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain l1 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain l1 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain B8 1–186（186 aa）
Chain b8 1–186（186 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB8_MOUSE
Isoform —
PDB entities 62
Chains and sequence ranges Author chain l1； PDBConstruct 1–186； UniProt 1–186

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

OrganismNot specified

UniProt Q9CQC7

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain m1； UniProt 1–129 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain m1 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain m1 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain B4 1–129（129 aa）
Chain b4 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB4_MOUSE
Isoform —
PDB entities 63
Chains and sequence ranges Author chain m1； PDBConstruct 1–129； UniProt 1–129

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

OrganismNot specified

UniProt Q9CQJ8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain n1； UniProt 1–179 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain n1 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain n1 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain B9 1–179（179 aa）
Chain b9 1–179（179 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB9_MOUSE
Isoform —
PDB entities 64
Chains and sequence ranges Author chain n1； PDBConstruct 1–179； UniProt 1–179

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

OrganismNot specified

UniProt Q9CR61

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain o1； UniProt 1–137 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 2–137（136 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain o1 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain o1 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain B7 2–137（136 aa）
Chain b7 2–137（136 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

29 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB7_MOUSE
Isoform —
PDB entities 65
Chains and sequence ranges Author chain o1； PDBConstruct 1–137； UniProt 1–137

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

OrganismNot specified

UniProt Q9DCS9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain p1； UniProt 1–176 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） Cytochrome c oxidase subunit 8A, mitochondrial × 1 （Q64445） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain p1 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain p1 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain BL 1–176（176 aa）
Chain bl 1–176（176 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUBA_MOUSE
Isoform —
PDB entities 66
Chains and sequence ranges Author chain p1； PDBConstruct 1–176； UniProt 1–176

Cytochrome c oxidase subunit 8A, mitochondrial

OrganismNot specified

UniProt Q64445

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 79 PDB declaration: 79-meric（79） Consistent with protein copy count Chain z； UniProt 1–69 Not recorded Cytochrome c oxidase subunit 1 × 1 （P00397） Cytochrome c oxidase subunit 2 × 1 （P00405） Cytochrome c oxidase subunit 3 × 1 （P00416） Cytochrome c oxidase subunit 4 isoform 1, mitochondrial × 1 （P19783） Cytochrome c oxidase subunit 5A, mitochondrial × 1 （P12787） Cytochrome c oxidase subunit 5B, mitochondrial × 1 （P19536） Cytochrome c oxidase subunit 6A1, mitochondrial × 1 （P43024） Cytochrome c oxidase subunit 6B1 × 1 （P56391） Cytochrome c oxidase subunit 6C × 1 （Q9CPQ1） Cytochrome c oxidase subunit 7B, mitochondrial × 1 （P56393） Cytochrome c oxidase subunit 7C, mitochondrial × 1 （P17665） Cytochrome c oxidase subunit 7A2, mitochondrial × 1 （P48771） Cytochrome b-c1 complex subunit 1, mitochondrial × 2 （Q9CZ13） Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （Q9DB77） Cytochrome b × 2 （P00158） Cytochrome c1, heme protein, mitochondrial × 2 （Q9D0M3） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 3 （Q9CR68） Cytochrome b-c1 complex subunit 7 × 2 （Q9CQB4） Cytochrome b-c1 complex subunit 8 × 2 （Q9CQ69） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P99028） Cytochrome b-c1 complex subunit 9 × 2 （Q8R1I1） Cytochrome b-c1 complex subunit 10 × 2 （Q9CPX8） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （Q9DC70） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （Q9DCT2） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （Q91WD5） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （Q9D6J6） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （Q91YT0） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （Q91VD9） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （Q8K3J1） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q9DC69） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q9CXZ1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P52503） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q9CQ75） Acyl carrier protein, mitochondrial × 2 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q9CPP6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q9CQZ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q7TMF3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q9Z1P6） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （Q8BK30） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03899） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03888） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03925） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03903） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03921） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03911） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03893） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （Q99LC3） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （Q9DCJ5） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （G5E814） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9ERS2） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O35683） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q9CQ91） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q9CQY9） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q9CQ54） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q99LY9） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （P0DN34） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （O09111） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q9CQH3） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q3UIU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q9CPU2） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q9CQZ6） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q9D6J5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （Q9CQC7） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9CQJ8） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q9CR61） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q9DCS9） CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.7 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8PW7 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain m 1–69（69 aa）
Chain z 1–69（69 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain z 1–69（69 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name COX8A_MOUSE
Isoform —
PDB entities 67
Chains and sequence ranges Author chain z； PDBConstruct 1–69； UniProt 1–69

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 8pw7

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 8pw7
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2. Structure Basics 2. Structure Basics

Entry ID entry_id8pw7
Deposition date deposition_date2023-07-19
Structure title titleA respirasome from murine liver
Keywords keywordsRespiratory chain super complex, mammalian mitochondria, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier88.89 Å
Radius of gyration Rg (electron density) rg_electron87.88 Å
Forward intensity I(0) i034338900000.00
Molecular weight molecular_weight1645400.0 kDa
Excluded volume excluded_volume2082300 ų
Envelope volume envelope_volume3089100 ų
Hydration-shell volume shell_volume282620 ų
Envelope diameter envelope_diameter303.0 Å
Shell Rg shell_rg92.08 Å
Envelope Rg envelope_rg85.54 Å
Shape Rg shape_rg87.90 Å
Total Rg total_rg87.82 Å
Total atoms total_atoms115575
Residues n_residues14142
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax224.3 Å
Rg (real space) rg_real85.69 Å
Rg uncertainty (real space) rg_real_error0.53 Å
I(0) (real space) i0_real3.2920e+10
I(0) uncertainty (real space) i0_real_error6.0940e+08
Rg (reciprocal space) rg_reciprocal89.52 Å
I(0) (reciprocal space) i0_reciprocal34400000000.0000
Solution quality estimate total_estimate0.9133
Solution quality rating solution_quality EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary118.8 Å
Skewness Skewness skewness0.064
Kurtosis Kurtosis kurtosis-0.684
Angular range angular_range— – 0.0850 Å−1
Current regularization parameter α current_alpha0.6846
Highest regularization parameter α highest_alpha1380000000.0000
Real-space data points n_real_points18
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.001; Oscil: 0.995; Stabil: 0.978; Sysdev: 1.000; Positv: 1.000; Valcen: 0.953; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (86)

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8. Citations (2)

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9. Files and Curves (10)