Current Protein Identity:P04386
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1AW6 GAL4 (CD), NMR, 24 STRUCTURES Deposited 1997-10-10 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–43(43 aa)
Fragment:DNA-BINDING DOMAIN
|
Not recorded | CD CADMIUM ION × 2 | SOLUTION NMR |
NMR measurement conditions
pH 7.2;298 K
|
Resolution not provided |
| 1D66 DNA RECOGNITION BY GAL4: STRUCTURE OF A PROTEIN/DNA COMPLEX Deposited 1992-03-06 | Assembly 1 Protein–DNA Homooligomer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
1–65(65 aa)
Chain B
1–65(65 aa)
|
Not recorded | CD CADMIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.8;pH 6.80, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.70 Å |
| 1HBW Solution nmr structure of the dimerization domain of the yeast transcriptional activator Gal4 (residues 50-106) Deposited 2001-04-20 | Assembly 1 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
50–106(57 aa)
Fragment:DIMERIZATION DOMAIN RESIDUES 50-106
Chain B
50–106(57 aa)
Fragment:DIMERIZATION DOMAIN RESIDUES 50-106
|
Mutation:YES Mutation:YES | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.4;308 K;Ionic strength (raw mmCIF value) 50 MM SODIUM PHOSPHATE
|
Resolution not provided |
| 3BTS Crystal structure of a ternary complex of the transcriptional repressor Gal80p (Gal80S0 [G301R]) and the acidic activation domain of Gal4p (aa 854-874) from Saccharomyces cerevisiae with NAD Deposited 2007-12-30 | Assembly 1 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count |
Chain E
854–874(21 aa)
Fragment:S. cerevisiae Gal4p peptide; UNP residues 854-874
Chain F
854–874(21 aa)
Fragment:S. cerevisiae Gal4p peptide; UNP residues 854-874
|
Not recorded | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;290 K;20% PEG 3350, 0.15M Sodium fluoride, followed by
soaking in NAD to a final concentration of 5mM, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 2.70 Å R-free 0.232 |
| 3COQ Structural Basis for Dimerization in DNA Recognition by Gal4 Deposited 2008-03-29 | Assembly 1 Protein–DNA Homooligomer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
8–96(89 aa)
Fragment:DNA binding domain with complete dimerization domain
Chain B
8–96(89 aa)
Fragment:DNA binding domain with complete dimerization domain
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION;pH 5.5;298 K;40mM Mg(OAc)2, 25mM sodium phosphate, 5% PEG400, 5% MPD, pH 5.5, VAPOR DIFFUSION, temperature 298K
|
Resolution 2.40 Å R-free 0.269 |
| 7UIK Mediator-PIC Early (Tail A + Upstream DNA & Activator) Deposited 2022-03-29 | Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers |
Chain T
1–147(147 aa)
Chain U
1–147(147 aa)
|
Not recorded | ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.70 Å |
| 7UIO Mediator-PIC Early (Composite Model) Deposited 2022-03-29 | Assembly 1 Protein–DNA Heteromer;Protein × 78 PDB declaration: 80-meric(80) Consistent with all polymers |
Chain GA
1–147(147 aa)
Chain GB
1–147(147 aa)
|
Not recorded | ZN ZINC ION × 4 THR THREONINE × 1 ALA ALANINE × 1 ASP ASPARTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |