7uik

Mediator-PIC Early (Tail A + Upstream DNA & Activator)

Method: ELECTRON MICROSCOPY
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Regulatory protein GAL4

Saccharomyces cerevisiae S288C

UniProt P04386

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 8 DNA 2 DNA (38-MER) × 1 DNA (37-MER) × 1 Mediator of RNA polymerase II transcription subunit 15 × 1 (P19659) Mediator of RNA polymerase II transcription subunit 2 × 1 (Q12124) Mediator of RNA polymerase II transcription subunit 3 × 1 (P40356) Mediator of RNA polymerase II transcription subunit 14 × 1 (P19263) Mediator of RNA polymerase II transcription subunit 5 × 1 (P53114) Mediator of RNA polymerase II transcription subunit 16 × 1 (P32259) ZINC ION × 4 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name GAL4_YEAST
Isoform —
PDB entities 3
Chains and sequence ranges Author chain T; PDBConstruct 1–147; UniProt 1–147 Author chain U; PDBConstruct 1–147; UniProt 1–147

Mediator of RNA polymerase II transcription subunit 15

OrganismNot specified

UniProt P19659

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 8 DNA 2 DNA (38-MER) × 1 DNA (37-MER) × 1 Regulatory protein GAL4 × 2 (P04386) Mediator of RNA polymerase II transcription subunit 2 × 1 (Q12124) Mediator of RNA polymerase II transcription subunit 3 × 1 (P40356) Mediator of RNA polymerase II transcription subunit 14 × 1 (P19263) Mediator of RNA polymerase II transcription subunit 5 × 1 (P53114) Mediator of RNA polymerase II transcription subunit 16 × 1 (P32259) ZINC ION × 4 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name MED15_YEAST
Isoform —
PDB entities 4
Chains and sequence ranges Author chain o; PDBConstruct 1–1081; UniProt 1–1081

Mediator of RNA polymerase II transcription subunit 2

OrganismNot specified

UniProt Q12124

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 8 DNA 2 DNA (38-MER) × 1 DNA (37-MER) × 1 Regulatory protein GAL4 × 2 (P04386) Mediator of RNA polymerase II transcription subunit 15 × 1 (P19659) Mediator of RNA polymerase II transcription subunit 3 × 1 (P40356) Mediator of RNA polymerase II transcription subunit 14 × 1 (P19263) Mediator of RNA polymerase II transcription subunit 5 × 1 (P53114) Mediator of RNA polymerase II transcription subunit 16 × 1 (P32259) ZINC ION × 4 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name MED2_YEAST
Isoform —
PDB entities 5
Chains and sequence ranges Author chain b; PDBConstruct 1–431; UniProt 1–431

Mediator of RNA polymerase II transcription subunit 3

OrganismNot specified

UniProt P40356

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 8 DNA 2 DNA (38-MER) × 1 DNA (37-MER) × 1 Regulatory protein GAL4 × 2 (P04386) Mediator of RNA polymerase II transcription subunit 15 × 1 (P19659) Mediator of RNA polymerase II transcription subunit 2 × 1 (Q12124) Mediator of RNA polymerase II transcription subunit 14 × 1 (P19263) Mediator of RNA polymerase II transcription subunit 5 × 1 (P53114) Mediator of RNA polymerase II transcription subunit 16 × 1 (P32259) ZINC ION × 4 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name MED3_YEAST
Isoform —
PDB entities 6
Chains and sequence ranges Author chain c; PDBConstruct 1–397; UniProt 1–397

Mediator of RNA polymerase II transcription subunit 14

OrganismNot specified

UniProt P19263

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 8 DNA 2 DNA (38-MER) × 1 DNA (37-MER) × 1 Regulatory protein GAL4 × 2 (P04386) Mediator of RNA polymerase II transcription subunit 15 × 1 (P19659) Mediator of RNA polymerase II transcription subunit 2 × 1 (Q12124) Mediator of RNA polymerase II transcription subunit 3 × 1 (P40356) Mediator of RNA polymerase II transcription subunit 5 × 1 (P53114) Mediator of RNA polymerase II transcription subunit 16 × 1 (P32259) ZINC ION × 4 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name MED14_YEAST
Isoform —
PDB entities 7
Chains and sequence ranges Author chain n; PDBConstruct 1–241; UniProt 834–1074

Mediator of RNA polymerase II transcription subunit 5

OrganismNot specified

UniProt P53114

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 8 DNA 2 DNA (38-MER) × 1 DNA (37-MER) × 1 Regulatory protein GAL4 × 2 (P04386) Mediator of RNA polymerase II transcription subunit 15 × 1 (P19659) Mediator of RNA polymerase II transcription subunit 2 × 1 (Q12124) Mediator of RNA polymerase II transcription subunit 3 × 1 (P40356) Mediator of RNA polymerase II transcription subunit 14 × 1 (P19263) Mediator of RNA polymerase II transcription subunit 16 × 1 (P32259) ZINC ION × 4 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name MED5_YEAST
Isoform —
PDB entities 8
Chains and sequence ranges Author chain e; PDBConstruct 1–1132; UniProt 1–1132

Mediator of RNA polymerase II transcription subunit 16

OrganismNot specified

UniProt P32259

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 8 DNA 2 DNA (38-MER) × 1 DNA (37-MER) × 1 Regulatory protein GAL4 × 2 (P04386) Mediator of RNA polymerase II transcription subunit 15 × 1 (P19659) Mediator of RNA polymerase II transcription subunit 2 × 1 (Q12124) Mediator of RNA polymerase II transcription subunit 3 × 1 (P40356) Mediator of RNA polymerase II transcription subunit 14 × 1 (P19263) Mediator of RNA polymerase II transcription subunit 5 × 1 (P53114) ZINC ION × 4 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name MED16_YEAST
Isoform —
PDB entities 9
Chains and sequence ranges Author chain p; PDBConstruct 1–974; UniProt 1–974

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id7uik
Deposition date deposition_date2022-03-29
Structure title titleMediator-PIC Early (Tail A + Upstream DNA & Activator)
Keywords keywords;Divergent transcription, Mediator, RNA Polymerase II, PIC, Pre-Initiation, Activation, Transcription Factor, Gal4, Gal4VP16, TRANSCRIPTION ;; TRANSCRIPTION
Experimental Method methodELECTRON MICROSCOPY
▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7uik__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7uik__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

7uik__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)57.95 Å
Rg (electron density)57.09 Å
Total Rg57.30 Å
Atom count20237
Residues2383
Excluded volume358830 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 7uik__assembly_1__model_1 decameric (10) Success 4.1.3-1-20251215 (887e7ef) View Download
▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (10)

▶

7. Citations (1)