Current Protein Identity：P06396
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1C0F CRYSTAL STRUCTURE OF DICTYOSTELIUM CAATP-ACTIN IN COMPLEX WITH GELSOLIN SEGMENT 1 Deposited 1999-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain S
53–176（124 aa）
|
Mutation：N35C | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；290 K；IMIDAZOLE, SODIUM CHLORIDE, ATP, CALCIUM CHLORIDE, MAGNESIUM CHLORIDE, SODIUM
AZIDE, DITHIOTHREITOL, PEG 6000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 2.40 Å R-free 0.231 |
| 1C0G CRYSTAL STRUCTURE OF 1:1 COMPLEX BETWEEN GELSOLIN SEGMENT 1 AND A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 228: Q228K/T229A/A230Y/E360H) Deposited 1999-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain S
53–176（124 aa）
|
Mutation：YES | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；MES, SODIUM CHLORIDE, ATP, CALCIUM CHLORIDE, MAGNESIUM CHLORIDE, SODIUM AZIDE,
DITHIOTHREITOL, PEG 6000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.00 Å R-free 0.223 |
| 1D4X Crystal Structure of Caenorhabditis Elegans Mg-ATP Actin Complexed with Human Gelsolin Segment 1 at 1.75 A resolution. Deposited 1999-10-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
52–177（126 aa）
Fragment：SEGMENT 1
|
Not recorded | MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SO2 SULFUR DIOXIDE × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100 MM TRIS-HCL, PH 8.0, 2 MM MG-ATP, 1.60 M LI2SO4,, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.75 Å R-free 0.233 |
| 1D4X Crystal Structure of Caenorhabditis Elegans Mg-ATP Actin Complexed with Human Gelsolin Segment 1 at 1.75 A resolution. Deposited 1999-10-06 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain G
52–177（126 aa）
Fragment：SEGMENT 1
|
Not recorded | MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SO2 SULFUR DIOXIDE × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100 MM TRIS-HCL, PH 8.0, 2 MM MG-ATP, 1.60 M LI2SO4,, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.75 Å R-free 0.233 |
| 1DEJ CRYSTAL STRUCTURE OF A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 646: Q228K/T229A/A230Y/A231K/S232E/E360H) IN COMPLEX WITH HUMAN GELSOLIN SEGMENT 1 Deposited 1999-11-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain S
53–176（124 aa）
Fragment：SEGMENT 1
|
Mutation：YES | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；MES, NACL, CACL2, MGCL2, ATP, PEG6000, DTT, NAN3, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.214 |
| 1EQY COMPLEX BETWEEN RABBIT MUSCLE ALPHA-ACTIN: HUMAN GELSOLIN DOMAIN 1 Deposited 2000-04-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain S
52–176（125 aa）
Fragment：DOMAIN 1
|
Mutation：N33C | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.6；298 K；PEG 6000, sodium chloride, adenosine triphosphate, calcium, magnesium, sodium azide, pH 6.6, Vapor Diffusion, temperature 298.0K
|
Resolution 2.30 Å R-free 0.280 |
| 1ESV COMPLEX BETWEEN LATRUNCULIN A:RABBIT MUSCLE ALPHA ACTIN:HUMAN GELSOLIN DOMAIN 1 Deposited 2000-04-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain S
52–176（125 aa）
Fragment：DOMAIN 1
|
Mutation：N33C | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.6；298 K；PEG 6000, sodium Chloride, adenosine triphosphate, calcium, magnesium, sodium azide, pH 6.6, VAPOR DIFFUSION, temperature 298.0K
|
Resolution 2.00 Å R-free 0.286 |
| 1H1V gelsolin G4-G6/actin complex Deposited 2002-07-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
439–769（331 aa）
Fragment：G4-G6, RESIDUES 412-742 OF CYTOPLASMIC ISOFORM
|
Not recorded | CA CALCIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；PROTEIN WAS CRYSTALLIZED FROM 100MM HEPES BUFFER, PH 7.5, 20% GLYCEROL, 10 % PEG 8000
|
Resolution 2.99 Å R-free 0.268 |
| 1KCQ Human Gelsolin Domain 2 with a Cd2+ bound Deposited 2001-11-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
185–288（104 aa）
Fragment：DOMAIN 2
|
Not recorded | CD CADMIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；290 K；PEG 400, cadmium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP at 290K
|
Resolution 1.65 Å R-free 0.233 |
| 1KCQ Human Gelsolin Domain 2 with a Cd2+ bound Deposited 2001-11-09 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
185–288（104 aa）
Fragment：DOMAIN 2
|
Not recorded | CD CADMIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；290 K；PEG 400, cadmium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP at 290K
|
Resolution 1.65 Å R-free 0.233 |
| 1MDU Crystal structure of the chicken actin trimer complexed with human gelsolin segment 1 (GS-1) Deposited 2002-08-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
52–176（125 aa）
Fragment：actin-severing
|
Not recorded | CA CALCIUM ION × 12 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；PEG 3350, tris, calcium chloride, magnesium chloride, ADP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.233 |
| 1MDU Crystal structure of the chicken actin trimer complexed with human gelsolin segment 1 (GS-1) Deposited 2002-08-07 | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain D
52–176（125 aa）
Fragment：actin-severing
|
Not recorded | CA CALCIUM ION × 9 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；PEG 3350, tris, calcium chloride, magnesium chloride, ADP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.233 |
| 1NLV Crystal Structure Of Dictyostelium Discoideum Actin Complexed With Ca ATP And Human Gelsolin Segment 1 Deposited 2003-01-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：DOMAIN 1
|
Not recorded | CA CALCIUM ION × 2 SO4 SULFATE ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SO2 SULFUR DIOXIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；lithium chloride, magnesium chloride, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.80 Å R-free 0.258 |
| 1NLV Crystal Structure Of Dictyostelium Discoideum Actin Complexed With Ca ATP And Human Gelsolin Segment 1 Deposited 2003-01-07 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：DOMAIN 1
|
Not recorded | CA CALCIUM ION × 4 SO4 SULFATE ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SO2 SULFUR DIOXIDE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；lithium chloride, magnesium chloride, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.80 Å R-free 0.258 |
| 1NM1 Crystal Structure of D. Dicsoideum Actin Complexed With Gelsolin Segment 1 and Mg ATP at 1.8 A Resolution Deposited 2003-01-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：DOMAIN 1
|
Not recorded | MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SO2 SULFUR DIOXIDE × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；Magnesium chloride, litheim chloride, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.80 Å R-free 0.232 |
| 1NM1 Crystal Structure of D. Dicsoideum Actin Complexed With Gelsolin Segment 1 and Mg ATP at 1.8 A Resolution Deposited 2003-01-08 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：DOMAIN 1
|
Not recorded | MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SO2 SULFUR DIOXIDE × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；Magnesium chloride, litheim chloride, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.80 Å R-free 0.232 |
| 1NMD Crystal Structure of D. Discoideum Actin-Gelsolin Segment 1 Complex Crystallized In Presence Of Lithium ATP Deposited 2003-01-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：domain I
|
Not recorded | SO4 SULFATE ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SO2 SULFUR DIOXIDE × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；300 K；Lithium sulfate, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 1.90 Å R-free 0.250 |
| 1NMD Crystal Structure of D. Discoideum Actin-Gelsolin Segment 1 Complex Crystallized In Presence Of Lithium ATP Deposited 2003-01-09 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：domain I
|
Not recorded | SO4 SULFATE ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SO2 SULFUR DIOXIDE × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；300 K；Lithium sulfate, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 1.90 Å R-free 0.250 |
| 1P8X The Calcium-Activated C-terminal half of gelsolin Deposited 2003-05-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
439–782（344 aa）
Fragment：C-terminal domain
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277.15 K；15% (w/v) PEG 8000, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K
|
Resolution 2.00 Å R-free 0.245 |
| 1P8X The Calcium-Activated C-terminal half of gelsolin Deposited 2003-05-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
439–782（344 aa）
Fragment：C-terminal domain
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277.15 K；15% (w/v) PEG 8000, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K
|
Resolution 2.00 Å R-free 0.245 |
| 1P8X The Calcium-Activated C-terminal half of gelsolin Deposited 2003-05-08 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
439–782（344 aa）
Fragment：C-terminal domain
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277.15 K；15% (w/v) PEG 8000, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K
|
Resolution 2.00 Å R-free 0.245 |
| 1P8Z Complex Between Rabbit Muscle alpha-Actin: Human Gelsolin Residues Val26-Glu156 Deposited 2003-05-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
52–187（136 aa）
|
Not recorded | CD CADMIUM ION × 3 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；0.1M Sodium Acetate, 10 mM Cadmium Chloride, 12.5% (v/v) PEG 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.263 |
| 1P8Z Complex Between Rabbit Muscle alpha-Actin: Human Gelsolin Residues Val26-Glu156 Deposited 2003-05-08 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain G
52–187（136 aa）
|
Not recorded | CD CADMIUM ION × 6 CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；0.1M Sodium Acetate, 10 mM Cadmium Chloride, 12.5% (v/v) PEG 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.263 |
| 1SOL A PIP2 AND F-ACTIN-BINDING SITE OF GELSOLIN, RESIDUE 150-169 (NMR, AVERAGED STRUCTURE) Deposited 1995-09-29 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
177–196（20 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR | mmCIF provides none of the parsed conditions | Resolution not provided |
| 1T44 Structural basis of actin sequestration by thymosin-B4: Implications for arp2/3 activation Deposited 2004-04-28 | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
55–179（125 aa）
Fragment：Chimera of Gelsolin domain 1 (residues 28-152) from human and C-Terminal domain of thymosin Beta-4 from mouse (residues 153-171)
|
Not recorded | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 6.5；293 K；PEG 8000, Sodium acetate, calcium chloride, pH 6.5, microbatch, temperature 293K
|
Resolution 2.00 Å R-free 0.195 |
| 1YAG STRUCTURE OF THE YEAST ACTIN-HUMAN GELSOLIN SEGMENT 1 COMPLEX Deposited 1998-10-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：SUBDOMAIN 1
|
Not recorded | MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.50
|
Resolution 1.90 Å R-free 0.231 |
| 1YAG STRUCTURE OF THE YEAST ACTIN-HUMAN GELSOLIN SEGMENT 1 COMPLEX Deposited 1998-10-09 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：SUBDOMAIN 1
|
Not recorded | MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.50
|
Resolution 1.90 Å R-free 0.231 |
| 1YVN THE YEAST ACTIN VAL 159 ASN MUTANT COMPLEX WITH HUMAN GELSOLIN SEGMENT 1. Deposited 1999-03-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：FRAGMENT 1
|
Not recorded | MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；0.1 M HEPES, 1.60 M (NH4)2SO4, 2 MM MG-ATP, PH=8.0.
|
Resolution 2.10 Å R-free 0.235 |
| 1YVN THE YEAST ACTIN VAL 159 ASN MUTANT COMPLEX WITH HUMAN GELSOLIN SEGMENT 1. Deposited 1999-03-23 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：FRAGMENT 1
|
Not recorded | MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；0.1 M HEPES, 1.60 M (NH4)2SO4, 2 MM MG-ATP, PH=8.0.
|
Resolution 2.10 Å R-free 0.235 |
| 2FF3 Crystal structure of Gelsolin domain 1:N-wasp V2 motif hybrid in complex with actin Deposited 2005-12-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
52–179（128 aa）
Fragment：Gelsolin domain 1
|
Not recorded | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 6.5；293.15 K；5% PEG 8000, 0.1M sodium acetate, 10mM calcium chloride, pH 6.5, microbatch, temperature 293.15K
|
Resolution 2.00 Å R-free 0.250 |
| 2FF6 Crystal structure of Gelsolin domain 1:ciboulot domain 2 hybrid in complex with actin Deposited 2005-12-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain G
51–178（128 aa）
Fragment：Gelsolin domain 1
|
Not recorded | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；7% PEG 3000, 0.1M Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 2.05 Å R-free 0.237 |
| 2FH1 C-terminal half of gelsolin soaked in low calcium at pH 4.5 Deposited 2005-12-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
439–782（344 aa）
Fragment：C-terminal half domain
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
|
Resolution 1.55 Å R-free 0.214 |
| 2FH1 C-terminal half of gelsolin soaked in low calcium at pH 4.5 Deposited 2005-12-23 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
439–782（344 aa）
Fragment：C-terminal half domain
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
|
Resolution 1.55 Å R-free 0.214 |
| 2FH1 C-terminal half of gelsolin soaked in low calcium at pH 4.5 Deposited 2005-12-23 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
439–782（344 aa）
Fragment：C-terminal half domain
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
|
Resolution 1.55 Å R-free 0.214 |
| 2FH2 C-terminal half of gelsolin soaked in EGTA at pH 4.5 Deposited 2005-12-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
439–782（344 aa）
Fragment：C-terminal half domain
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
|
Resolution 2.50 Å R-free 0.255 |
| 2FH2 C-terminal half of gelsolin soaked in EGTA at pH 4.5 Deposited 2005-12-23 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
439–782（344 aa）
Fragment：C-terminal half domain
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
|
Resolution 2.50 Å R-free 0.255 |
| 2FH2 C-terminal half of gelsolin soaked in EGTA at pH 4.5 Deposited 2005-12-23 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
439–782（344 aa）
Fragment：C-terminal half domain
|
Not recorded | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
|
Resolution 2.50 Å R-free 0.255 |
| 2FH3 C-terminal half of gelsolin soaked in low calcium at pH 8 Deposited 2005-12-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
439–782（344 aa）
Fragment：C-terminal half domain
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
|
Resolution 2.87 Å R-free 0.262 |
| 2FH3 C-terminal half of gelsolin soaked in low calcium at pH 8 Deposited 2005-12-23 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
439–782（344 aa）
Fragment：C-terminal half domain
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
|
Resolution 2.87 Å R-free 0.262 |
| 2FH3 C-terminal half of gelsolin soaked in low calcium at pH 8 Deposited 2005-12-23 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
439–782（344 aa）
Fragment：C-terminal half domain
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
|
Resolution 2.87 Å R-free 0.262 |
| 2FH4 C-terminal half of gelsolin soaked in EGTA at pH 8 Deposited 2005-12-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
439–782（344 aa）
Fragment：C-terminal half domain
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
|
Resolution 3.00 Å R-free 0.310 |
| 2FH4 C-terminal half of gelsolin soaked in EGTA at pH 8 Deposited 2005-12-23 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
439–782（344 aa）
Fragment：C-terminal half domain
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
|
Resolution 3.00 Å R-free 0.310 |
| 2FH4 C-terminal half of gelsolin soaked in EGTA at pH 8 Deposited 2005-12-23 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
439–782（344 aa）
Fragment：C-terminal half domain
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
|
Resolution 3.00 Å R-free 0.310 |
| 3A5L Crystal Structure of a Dictyostelium P109A Mg2+-Actin in Complex with Human Gelsolin Segment 1 Deposited 2009-08-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain S
53–176（124 aa）
Fragment：GELSOLIN-LIKE 1, residues 53-176
|
Mutation：N35C | CA CALCIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.207 |
| 3A5M Crystal Structure of a Dictyostelium P109I Mg2+-Actin in Complex with Human Gelsolin Segment 1 Deposited 2009-08-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain S
53–176（124 aa）
Fragment：GELSOLIN-LIKE 1, residues 53-176
|
Mutation：N35C | CA CALCIUM ION × 2 MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.223 |
| 3A5N Crystal Structure of a Dictyostelium P109A Ca2+-Actin in Complex with Human Gelsolin Segment 1 Deposited 2009-08-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain S
53–176（124 aa）
Fragment：GELSOLIN-LIKE 1, residues 53-176
|
Mutation：N35C | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.36 Å R-free 0.204 |
| 3A5O Crystal Structure of a Dictyostelium P109I Ca2+-Actin in Complex with Human Gelsolin Segment 1 Deposited 2009-08-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain S
53–176（124 aa）
Fragment：GELSOLIN-LIKE 1, residues 53-176
|
Mutation：N35C | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.207 |
| 3CI5 Complex of Phosphorylated Dictyostelium Discoideum Actin with Gelsolin Deposited 2008-03-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：Gelsolin Segment 1 (UNP residues 52-176)
|
Not recorded | MG MAGNESIUM ION × 1 SO4 SULFATE ION × 10 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GOL GLYCEROL × 5 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M HEPES, 1.7M Li2SO4, 2mM ATP, 1mM EDTA, 10% Glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.191 |
| 3CI5 Complex of Phosphorylated Dictyostelium Discoideum Actin with Gelsolin Deposited 2008-03-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：Gelsolin Segment 1 (UNP residues 52-176)
|
Not recorded | MG MAGNESIUM ION × 2 SO4 SULFATE ION × 20 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 GOL GLYCEROL × 10 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M HEPES, 1.7M Li2SO4, 2mM ATP, 1mM EDTA, 10% Glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.191 |
| 3CIP Complex of Dictyostelium Discoideum Actin with Gelsolin Deposited 2008-03-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：Gelsolin Segment 1 (UNP RESIDUES 52-176)
|
Not recorded | MG MAGNESIUM ION × 1 SO4 SULFATE ION × 9 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GOL GLYCEROL × 3 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M HEPES, pH7.5, 1.7M Li2SO4, 2mM ATP, 1mM EDTA, 5% Glycerol, vapor diffusion, hanging drop, temperature 293K
|
Resolution 1.60 Å R-free 0.197 |
| 3CIP Complex of Dictyostelium Discoideum Actin with Gelsolin Deposited 2008-03-11 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：Gelsolin Segment 1 (UNP RESIDUES 52-176)
|
Not recorded | MG MAGNESIUM ION × 2 SO4 SULFATE ION × 18 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 GOL GLYCEROL × 6 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M HEPES, pH7.5, 1.7M Li2SO4, 2mM ATP, 1mM EDTA, 5% Glycerol, vapor diffusion, hanging drop, temperature 293K
|
Resolution 1.60 Å R-free 0.197 |
| 3CJB Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with Gelsolin-segment 1 Deposited 2008-03-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：segment 1
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；10% Peg MME 5000, 5% Tacsimate, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.21 Å R-free 0.269 |
| 3CJC Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with DNase I and Gelsolin-segment 1 Deposited 2008-03-12 | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain G
52–176（125 aa）
Fragment：segment 1
|
Not recorded | CA CALCIUM ION × 2 SO4 SULFATE ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.5 M (NH4)SO4, 0.1 M Bis-Tris, 0.1 M NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.90 Å R-free 0.278 |
| 3FFK Crystal structure of human Gelsolin domains G1-G3 bound to Actin Deposited 2008-12-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
52–426（375 aa）
Fragment：UNP residues 52-426
|
Not recorded | CA CALCIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；297 K；9% PEG 4000, 100 mM Sodium Acetate, 100 mM Calcium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.273 |
| 3FFK Crystal structure of human Gelsolin domains G1-G3 bound to Actin Deposited 2008-12-03 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
52–426（375 aa）
Fragment：UNP residues 52-426
|
Not recorded | CA CALCIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；297 K；9% PEG 4000, 100 mM Sodium Acetate, 100 mM Calcium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.273 |
| 3FFN Crystal structure of calcium-free human gelsolin Deposited 2008-12-04 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–782（782 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；297 K；15% glycerol, 100 mM Bis-Tris HCl, 1.5M ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.271 |
| 3FFN Crystal structure of calcium-free human gelsolin Deposited 2008-12-04 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–782（782 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；297 K；15% glycerol, 100 mM Bis-Tris HCl, 1.5M ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.271 |
| 3TU5 Actin complex with Gelsolin Segment 1 fused to Cobl segment Deposited 2011-09-15 | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
53–174（122 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；10% (w/v) polyethyleneglycol 20000, 2% (v/v) dioxane, and 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.00 Å R-free 0.204 |
| 4PKG Complex of ATP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
12–136（125 aa）
Fragment：Gelsolin (UNP residues 12-136), GGSGGSGGS linker, Tmod1 Actin-binding site 1 (UNP residues 50-101)
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 1.80 Å R-free 0.187 |
| 4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
12–136（125 aa）
Fragment：Gelsolin (UNP residues 12-136), GGSGGSGGS linker, Tmod1 Actin-binding site 1 (UNP residues 50-101)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å R-free 0.326 |
| 4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
12–136（125 aa）
Fragment：Gelsolin (UNP residues 12-136), GGSGGSGGS linker, Tmod1 Actin-binding site 1 (UNP residues 50-101)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å R-free 0.326 |
| 4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Assembly 3 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
12–136（125 aa）
Fragment：Gelsolin (UNP residues 12-136), GGSGGSGGS linker, Tmod1 Actin-binding site 1 (UNP residues 50-101)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å R-free 0.326 |
| 4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Assembly 4 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain J
12–136（125 aa）
Fragment：Gelsolin (UNP residues 12-136), GGSGGSGGS linker, Tmod1 Actin-binding site 1 (UNP residues 50-101)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å R-free 0.326 |
| 4PKI Complex of ATP-actin With the C-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
12–136（125 aa）
Fragment：Gelsolin (UNP residues 12-136), GGSGGSGGS linker, Tmod1 Actin-binding site 2 (UNP residues 160-349)
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.25 M sodium chloride, 12% w/v PEG3350
|
Resolution 2.30 Å R-free 0.201 |
| 4S10 Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis Deposited 2015-01-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
186–288（103 aa）
Fragment：GELSOLIN DOMAIN 2
|
Mutation：S226D, R228D | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；288 K；30% PEG 1500, VAPOR DIFFUSION, temperature 288K
|
Resolution 2.61 Å R-free 0.312 |
| 4S10 Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis Deposited 2015-01-07 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
186–288（103 aa）
Fragment：GELSOLIN DOMAIN 2
|
Mutation：S226D, R228D | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；288 K；30% PEG 1500, VAPOR DIFFUSION, temperature 288K
|
Resolution 2.61 Å R-free 0.312 |
| 4Z94 Actin Complex With a Chimera of Tropomodulin-1 and Leiomodin-1 Actin-Binding Site 2 Deposited 2015-04-09 | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
12–136（125 aa）
Fragment：;Gelsolin residues 12-136 (UNP), linker,Tropomodulin-1 residues 160-228 (UNP), Leiomodin-1 Actin-Binding Site 2 (UNP residues 364-486)
;
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.8；291.5 K；20% PEG3350, 200 mM lithium sulfate, 100 mM Tris, pH 8.8, 15% glycerol
|
Resolution 2.40 Å R-free 0.243 |
| 5FAE N184K pathological variant of gelsolin domain 2 (trigonal form) Deposited 2015-12-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
178–293（116 aa）
Fragment：UNP residues 178-293
|
Mutation：N184K | CA CALCIUM ION × 1 SO4 SULFATE ION × 4 PEG DI(HYDROXYETHYL)ETHER × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.2 M lithium sulfate
0.1 M tris
40% PEG 400
|
Resolution 1.70 Å R-free 0.225 |
| 5FAF N184K pathological variant of gelsolin domain 2 (orthorhombic form) Deposited 2015-12-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
178–293（116 aa）
Fragment：UNP residues 178-293
|
Mutation：N184K | CA CALCIUM ION × 2 GOL GLYCEROL × 3 CL CHLORIDE ION × 2 ACT ACETATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；20% PEG 8000
0.1 M MES
0.2 M calcium acetate
|
Resolution 1.05 Å R-free 0.168 |
| 5H3M Solution structure of human Gelsolin protein domain 1 at pH 5.0 Deposited 2016-10-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
55–187（133 aa）
Fragment：UNP residues 55-187
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5；298 K；Ionic strength (raw mmCIF value) 0；Pressure 1
NMR sample composition
0.65 mM 13C, 15N gelsolin domain 1, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 5H3N Solution structure of human Gelsolin protein domain 1 at pH 7.3 Deposited 2016-10-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
55–187（133 aa）
Fragment：UNP residues 55-187
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.3；298 K；Ionic strength (raw mmCIF value) 0；Pressure 1
NMR sample composition
0.70 mM 13C, 15N human Gelsolin, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 5O2Z Domain swap dimer of the G167R variant of gelsolin second domain Deposited 2017-05-23 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
178–293（116 aa）
Chain B
178–293（116 aa）
|
Mutation：G167R Mutation：G167R | CA CALCIUM ION × 2 FLC CITRATE ANION × 2 ACT ACETATE ION × 2 NA SODIUM ION × 2 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.6；293 K；0.2M ammonium acetate, 0.1M sodium citrate, 30% PEG 4000
|
Resolution 1.70 Å R-free 0.242 |
| 5UBO Mical-oxidized Actin complex with Gelsolin Segment 1 Deposited 2016-12-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain S
12–138（127 aa）
Fragment：unp residues 12-138
|
Not recorded | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；298 K；PEG 6000, NaCl, imidazole, ATP, calcium
|
Resolution 2.39 Å R-free 0.191 |
| 5ZZ0 HUMAN GELSOLIN FROM RESIDUES GLU28 TO ARG161 WITH CALCIUM Deposited 2018-05-29 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain G
55–188（134 aa）
Fragment：UNP residues 55-188
|
Not recorded | CA CALCIUM ION × 2 PG0 2-(2-METHOXYETHOXY)ETHANOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；Peg 6000 (20%), 100mM Sodium acetate, 200mM Calcium Chloride Dihydrate, pH 5
|
Resolution 2.63 Å R-free 0.254 |
| 5ZZ0 HUMAN GELSOLIN FROM RESIDUES GLU28 TO ARG161 WITH CALCIUM Deposited 2018-05-29 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
55–188（134 aa）
Fragment：UNP residues 55-188
|
Not recorded | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；Peg 6000 (20%), 100mM Sodium acetate, 200mM Calcium Chloride Dihydrate, pH 5
|
Resolution 2.63 Å R-free 0.254 |
| 6H1F Structure of the nanobody-stabilized gelsolin D187N variant (second domain) Deposited 2018-07-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
178–293（116 aa）
|
Mutation：D187N | SCN THIOCYANATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；0.1 M Potassium thiocyanate, 30%w/v PEG 2000 MME
|
Resolution 1.90 Å R-free 0.233 |
| 6JCO Crystal structure of calcium free human gelsolin amyloid mutant D187N Deposited 2019-01-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
56–782（727 aa）
Chain B
56–782（727 aa）
|
Mutation：D187N Mutation：D187N | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；298.1 K；15% glycerol, 1.5M ammonium sulfate, 0.1M tris HCl pH8.5
|
Resolution 2.88 Å R-free 0.267 |
| 6JEG Crystal structure of calcium free human gelsolin amyloid mutant G167R Deposited 2019-02-05 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
54–782（729 aa）
Chain B
54–782（729 aa）
|
Mutation：G167R Mutation：G167R | GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；298 K；15% glycerol, 1.5M ammonium sulfate, 0.1M tris HCl, pH8.5
|
Resolution 2.98 Å R-free 0.237 |
| 6JEH Crystal structure of calcium free human gelsolin amyloid mutant D187Y Deposited 2019-02-05 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
56–782（727 aa）
Chain B
56–782（727 aa）
|
Mutation：D187Y Mutation：D187Y | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；298 K；15% glycerol, 1.5M ammonium sulfate, 0.1M tris HCl pH8.5
|
Resolution 2.95 Å R-free 0.249 |
| 6LJE Crystal structure of gelsolin G3 domain (calcium and magnesium condition) Deposited 2019-12-14 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
297–397（101 aa）
Fragment：UNP residues 297-397
|
Not recorded | GOL GLYCEROL × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG3350, ammonium sulphate, calcium chloride, magnesium chloride
|
Resolution 1.40 Å R-free 0.189 |
| 6LJE Crystal structure of gelsolin G3 domain (calcium and magnesium condition) Deposited 2019-12-14 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
297–397（101 aa）
Fragment：UNP residues 297-397
|
Not recorded | GOL GLYCEROL × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG3350, ammonium sulphate, calcium chloride, magnesium chloride
|
Resolution 1.40 Å R-free 0.189 |
| 6LJF Crystal structure of gelsolin G3 domain (calcium condition) Deposited 2019-12-14 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
297–397（101 aa）
Fragment：UNP residues 297-397
|
Not recorded | CA CALCIUM ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG3350, ammonium sulphate, calcium chloride
|
Resolution 1.50 Å R-free 0.183 |
| 6LJF Crystal structure of gelsolin G3 domain (calcium condition) Deposited 2019-12-14 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
297–397（101 aa）
Fragment：UNP residues 297-397
|
Not recorded | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG3350, ammonium sulphate, calcium chloride
|
Resolution 1.50 Å R-free 0.183 |
| 6Q9R Crystal structure of the pathological N184K variant of calcium-free human gelsolin Deposited 2018-12-18 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
28–782（755 aa）
|
Mutation：N184K | CL CHLORIDE ION × 6 GOL GLYCEROL × 8 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；1.5 M ammonium sulfate, 15 % glycerol, 100 mM Tris-HCl
|
Resolution 2.73 Å R-free 0.260 |
| 6Q9R Crystal structure of the pathological N184K variant of calcium-free human gelsolin Deposited 2018-12-18 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
28–782（755 aa）
|
Mutation：N184K | CL CHLORIDE ION × 3 GOL GLYCEROL × 9 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；1.5 M ammonium sulfate, 15 % glycerol, 100 mM Tris-HCl
|
Resolution 2.73 Å R-free 0.260 |
| 6Q9Z Crystal structure of the pathological G167R variant of calcium-free human gelsolin, Deposited 2018-12-18 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
28–782（755 aa）
|
Mutation：G167R | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；1.5 M ammonium sulfate, 20% glycerol, 100 mM Tris-HCl
|
Resolution 3.80 Å R-free 0.264 |
| 6Q9Z Crystal structure of the pathological G167R variant of calcium-free human gelsolin, Deposited 2018-12-18 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
28–782（755 aa）
|
Mutation：G167R | SO4 SULFATE ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；1.5 M ammonium sulfate, 20% glycerol, 100 mM Tris-HCl
|
Resolution 3.80 Å R-free 0.264 |
| 6QBF Crystal structure of the pathological D187N variant of calcium-free human gelsolin. Deposited 2018-12-21 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
28–782（755 aa）
|
Mutation：D187N | SO4 SULFATE ION × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；1.3 M Ammonium sulfate, 19% glycerol, 100 mM Tris HCl
|
Resolution 3.50 Å R-free 0.276 |
| 6QBF Crystal structure of the pathological D187N variant of calcium-free human gelsolin. Deposited 2018-12-21 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
28–782（755 aa）
|
Mutation：D187N | SO4 SULFATE ION × 1 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；1.3 M Ammonium sulfate, 19% glycerol, 100 mM Tris HCl
|
Resolution 3.50 Å R-free 0.276 |
| 6QW3 Calcium-bound gelsolin domain 2 Deposited 2019-03-05 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
178–293（116 aa）
|
Not recorded | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；36% PEG5000 MME, 0.1M Sodium Acetate
|
Resolution 1.30 Å R-free 0.174 |
| 7P2B Crystal structure of human gelsolin amyloid mutant A551P Deposited 2021-07-05 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
28–782（755 aa）
|
Mutation：A551P | SO4 SULFATE ION × 4 GOL GLYCEROL × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；15% glycerol, 2.0 M ammonium sulfate, 0.1M tris HCl, pH8.5
|
Resolution 3.00 Å R-free 0.263 |
| 7P2B Crystal structure of human gelsolin amyloid mutant A551P Deposited 2021-07-05 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
28–782（755 aa）
|
Mutation：A551P | SO4 SULFATE ION × 3 GOL GLYCEROL × 3 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；15% glycerol, 2.0 M ammonium sulfate, 0.1M tris HCl, pH8.5
|
Resolution 3.00 Å R-free 0.263 |
| 8VIZ Structure of full-length gelsolin bound to the barbed end of F-actin Deposited 2024-01-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain G
52–782（731 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 14 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.63 Å |
| 8VKH Structure of gelsolin domains G1G3 bound to the barbed end of F-actin Deposited 2024-01-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain G
52–782（731 aa）
Chain H
52–782（731 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 CA CALCIUM ION × 14 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.63 Å |
| 9UG2 Severed and capped actin fragment by two G1G3 domains of gelsolin Deposited 2025-04-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric（9） Consistent with protein count |
Chain A
54–401（348 aa）
Chain B
54–401（348 aa）
|
Not recorded | CA CALCIUM ION × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 5 PO4 PHOSPHATE ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.89 Å |