Current Protein Identity：P07273
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1ENW ELONGATION FACTOR TFIIS DOMAIN II Deposited 2000-03-21 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
131–240（110 aa）
Fragment：DOMAIN II (RESIDUES 131-240)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；270 K；Ionic strength (raw mmCIF value) 50 mM；Pressure ambient
NMR sample composition
2 mM TFIIS; 5mM phosphate buffer; 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition
2 mM TFIIS; 5mM phosphate buffer; 99.996% D2O | 99.996% D2O
|
Resolution not provided |
| 1EO0 CONSERVED DOMAIN COMMON TO TRANSCRIPTION FACTORS TFIIS, ELONGIN A, CRSP70 Deposited 2000-03-21 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–77（77 aa）
Fragment：DOMAIN I
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.5；273 K；Ionic strength (raw mmCIF value) 300 mM NaCl；Pressure ambient
NMR sample composition
2 mM TFIIS1-111; 10 mM phosphate buffer; 90% H2O, 10%D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 1PQV RNA polymerase II-TFIIS complex Deposited 2003-06-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric（13） Consistent with protein count |
Chain S
1–309（309 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 9 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；sodium-ammonium tartrate, Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.80 Å |
| 1Y1V Refined RNA Polymerase II-TFIIS complex Deposited 2004-11-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric（13） Consistent with protein count |
Chain S
131–309（179 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.80 Å R-free 0.294 |
| 1Y1Y RNA Polymerase II-TFIIS-DNA/RNA complex Deposited 2004-11-19 | Assembly 1 Other combination Heteromer；Protein × 13 PDB declaration: pentadecameric（15） Consistent with all polymers |
Chain S
131–309（179 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 4.00 Å |
| 3GTM Co-complex of Backtracked RNA polymerase II with TFIIS Deposited 2009-03-27 | Assembly 1 Other combination Heteromer；Protein × 11 PDB declaration: tetradecameric（14） Consistent with all polymers |
Chain S
147–306（160 aa）
Fragment：Transcription Factor IIS E291H mutation, UNP residues 147-309
|
Mutation：E291H | MG MAGNESIUM ION × 1 ZN ZINC ION × 9 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；390mM (NH4)2HPO4/NaH2PO4, pH 6.0, 50mM Dioxane, 10mM DTT, 9-11% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.80 Å R-free 0.289 |
| 3PO3 Arrested RNA Polymerase II reactivation intermediate Deposited 2010-11-21 | Assembly 1 Other combination Heteromer；Protein × 13 PDB declaration: hexadecameric（16） Consistent with all polymers |
Chain S
132–309（178 aa）
|
Mutation：D290A | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 ACT ACETATE ION × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PGE TRIETHYLENE GLYCOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；4-7% PEG6000, 50mM HEPES pH 7.0, 200mM AMMONIUM ACETATE, 300mM SODIUM ACETATE, 5mM TCEP
, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.30 Å R-free 0.189 |
| 5FMF the P-lobe of RNA polymerase II pre-initiation complex Deposited 2015-11-03 | Assembly 1 Protein–DNA Heteromer；Protein × 25 PDB declaration: 27-meric（27） Consistent with all polymers |
Chain 2
136–309（174 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 MM HEPES (PH7.6), 5 MM DTT, 2 MM MG(OAC)2,AND 40 MM KOAC；pH 7.6；20 MM HEPES (PH7.6), 5 MM DTT, 2 MM MG(OAC)2,AND 40 MM KOAC
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 120, INSTRUMENT- FEI VITROBOT MARK III,
|
Resolution 6.00 Å |
| 7FAW Structure of LW domain from Yeast Deposited 2021-07-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–73（73 aa）
Fragment：LW domain
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；0.1M Tris, pH 8.5, 25% PEG 3350
|
Resolution 2.44 Å R-free 0.240 |
| 7FAW Structure of LW domain from Yeast Deposited 2021-07-07 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–73（73 aa）
Fragment：LW domain
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；0.1M Tris, pH 8.5, 25% PEG 3350
|
Resolution 2.44 Å R-free 0.240 |
| 7FAW Structure of LW domain from Yeast Deposited 2021-07-07 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
1–73（73 aa）
Fragment：LW domain
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；0.1M Tris, pH 8.5, 25% PEG 3350
|
Resolution 2.44 Å R-free 0.240 |
| 7UI9 Core Mediator-PICearly (Copy A) Deposited 2022-03-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric（33） Consistent with protein count |
Chain S
1–309（309 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7UIF Mediator-PIC Early (Core B) Deposited 2022-03-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric（33） Consistent with protein count |
Chain S
1–309（309 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 7UIO Mediator-PIC Early (Composite Model) Deposited 2022-03-29 | Assembly 1 Protein–DNA Heteromer；Protein × 78 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain AS
1–309（309 aa）
Chain BS
1–309（309 aa）
|
Not recorded | ZN ZINC ION × 4 THR THREONINE × 1 ALA ALANINE × 1 ASP ASPARTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8UOQ Composite map of PIC_delta_TFIIK form2 Deposited 2023-10-20 | Assembly 1 Protein–DNA Heteromer；Protein × 28 PDB declaration: 30-meric（30） Consistent with all polymers |
Chain S
1–309（309 aa）
|
Not recorded | ZN ZINC ION × 15 MG MAGNESIUM ION × 2 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8UOT Composite map of PICdeltaTFIIK form1 Deposited 2023-10-20 | Assembly 1 Protein–DNA Heteromer；Protein × 28 PDB declaration: 30-meric（30） Consistent with all polymers |
Chain S
1–309（309 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 15 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6；20 mM HEPES PH 7.6
50 mM KOAc
5 mM DTT
2 mM MgOAc
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |