当前蛋白身份：P07329
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 11NA Azotobacter vinelandii MoFeP (C1 symmetry) determined using the SPT Labtech chameleon in the presence of 0x SurfACT 提交 2026-03-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.69 Å |
| 11NB Azotobacter vinelandii MoFeP (C2 symmetry) determined using the SPT Labtech chameleon in the presence of 0x SurfACT 提交 2026-03-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.46 Å |
| 11NC Azotobacter vinelandii MoFeP (C1 symmetry) determined using the SPT Labtech chameleon in the presence of 0.25x SurfACT 提交 2026-03-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.16 Å |
| 11ND Azotobacter vinelandii MoFeP (C2 symmetry) determined using the SPT Labtech chameleon in the presence of 0.25x SurfACT 提交 2026-03-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.06 Å |
| 11NE Azotobacter vinelandii MoFeP (C1 symmetry) determined using the SPT Labtech chameleon in the presence of 1x SurfACT 提交 2026-03-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
2–523（522 aa）
链 D
2–523（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.49 Å |
| 11NF Azotobacter vinelandii MoFeP (C2 symmetry) determined using the SPT Labtech chameleon in the presence of 1x SurfACT 提交 2026-03-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.34 Å |
| 1FP4 CRYSTAL STRUCTURE OF THE ALPHA-H195Q MUTANT OF NITROGENASE 提交 2000-08-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CFM FE-MO-S CLUSTER × 2 CLP FE-S CLUSTER × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
microcapillary batch diffusion；pH 8；298 K；PEG 4000, TRIS pH 8.0, sodium molybdate, microcapillary batch diffusion, temperature 298K
|
分辨率 2.50 Å R-free 0.240 |
| 1G20 MGATP-BOUND AND NUCLEOTIDE-FREE STRUCTURES OF A NITROGENASE PROTEIN COMPLEX BETWEEN LEU127DEL-FE PROTEIN AND THE MOFE PROTEIN 提交 2000-10-16 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 B
1–522（522 aa）
链 D
1–522（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CFM FE-MO-S CLUSTER × 2 CA CALCIUM ION × 2 CLF FE(8)-S(7) CLUSTER × 2 SF4 IRON/SULFUR CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
microcapillary batch diffusion；pH 8.5；298 K；PEG 4000, sodium acetate, Tris-HCl. Ph 8.8, pH 8.5, microcapillary batch diffusion, temperature 298K
|
分辨率 2.20 Å R-free 0.255 |
| 1G21 MGATP-BOUND AND NUCLEOTIDE-FREE STRUCTURES OF A NITROGENASE PROTEIN COMPLEX BETWEEN LEU127DEL-FE PROTEIN AND THE MOFE PROTEIN 提交 2000-10-16 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CFM FE-MO-S CLUSTER × 2 CLF FE(8)-S(7) CLUSTER × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 SF4 IRON/SULFUR CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
microcapillary batch diffusion；pH 8.5；298 K；PEG 4000, sodium acetate, Tris-HCl. Ph 8.8, pH 8.5, microcapillary batch diffusion, temperature 298K
|
分辨率 3.00 Å R-free 0.285 |
| 1L5H FeMo-cofactor Deficient Nitrogenase MoFe Protein 提交 2002-03-06 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–522（522 aa）
|
未记录 | CA CALCIUM ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 9.5；298 K；PEG 8000, CHES, pH 9.5, LIQUID DIFFUSION, temperature 298K
|
分辨率 2.30 Å R-free 0.289 |
| 1M1N Nitrogenase MoFe protein from Azotobacter vinelandii 提交 2002-06-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–522（522 aa）
链 D
1–522（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CFN FE(7)-MO-S(9)-N CLUSTER × 2 CA CALCIUM ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；PEG 8000, sodium chloride, TRIS, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.16 Å R-free 0.149 |
| 1M1N Nitrogenase MoFe protein from Azotobacter vinelandii 提交 2002-06-19 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 F
1–522（522 aa）
链 H
1–522（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CFN FE(7)-MO-S(9)-N CLUSTER × 2 CA CALCIUM ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；PEG 8000, sodium chloride, TRIS, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.16 Å R-free 0.149 |
| 1M1Y Chemical Crosslink of Nitrogenase MoFe Protein and Fe Protein 提交 2002-06-20 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 B
1–522（522 aa）
链 D
1–522（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CFM FE-MO-S CLUSTER × 2 CLF FE(8)-S(7) CLUSTER × 2 CA CALCIUM ION × 2 SF4 IRON/SULFUR CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 8；298 K；PEG 6000, NaCl, Cadaverine, Tris, pH 8.0, LIQUID DIFFUSION, temperature 298K
|
分辨率 3.20 Å R-free 0.329 |
| 1M1Y Chemical Crosslink of Nitrogenase MoFe Protein and Fe Protein 提交 2002-06-20 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 J
1–522（522 aa）
链 L
1–522（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CFM FE-MO-S CLUSTER × 2 CLF FE(8)-S(7) CLUSTER × 2 CA CALCIUM ION × 2 SF4 IRON/SULFUR CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 8；298 K；PEG 6000, NaCl, Cadaverine, Tris, pH 8.0, LIQUID DIFFUSION, temperature 298K
|
分辨率 3.20 Å R-free 0.329 |
| 1N2C NITROGENASE COMPLEX FROM AZOTOBACTER VINELANDII STABILIZED BY ADP-TETRAFLUOROALUMINATE 提交 1997-05-02 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 B
1–522（522 aa）
片段：CHAINS A AND C ARE THE ALPHA CHAINS, CHAINS B AND D ARE THE BETA CHAINS
链 D
1–522（522 aa）
片段：CHAINS A AND C ARE THE ALPHA CHAINS, CHAINS B AND D ARE THE BETA CHAINS
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CFM FE-MO-S CLUSTER × 2 CA CALCIUM ION × 2 CLF FE(8)-S(7) CLUSTER × 2 MG MAGNESIUM ION × 4 ALF TETRAFLUOROALUMINATE ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 4 SF4 IRON/SULFUR CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；16-20% PEG 8000, 0.1 M CACODYLATE PH 6.5 AND 20-50 MM MGCL2
|
分辨率 3.00 Å R-free 0.238 |
| 2AFH Crystal Structure of Nucleotide-Free Av2-Av1 Complex 提交 2005-07-25 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 B
1–522（522 aa）
链 D
1–522（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CFN FE(7)-MO-S(9)-N CLUSTER × 2 PGE TRIETHYLENE GLYCOL × 8 CA CALCIUM ION × 2 NA SODIUM ION × 1 CLF FE(8)-S(7) CLUSTER × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 PG4 TETRAETHYLENE GLYCOL × 2 1PE PENTAETHYLENE GLYCOL × 2 PEG DI(HYDROXYETHYL)ETHER × 3 P6G HEXAETHYLENE GLYCOL × 1 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；PEG 10000, Tris, sodium chloride, magnesium chloride, dithionite, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298 K
|
分辨率 2.10 Å R-free 0.221 |
| 2AFI Crystal Structure of MgADP bound Av2-Av1 Complex 提交 2005-07-25 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 B
1–522（522 aa）
链 D
1–522（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CFN FE(7)-MO-S(9)-N CLUSTER × 2 CA CALCIUM ION × 2 CLF FE(8)-S(7) CLUSTER × 2 MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 4 SF4 IRON/SULFUR CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；PEG8000, Sodium Chloride, Magnesium Chloride, ADP, Dithionite, TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298 K
|
分辨率 3.10 Å R-free 0.270 |
| 2AFI Crystal Structure of MgADP bound Av2-Av1 Complex 提交 2005-07-25 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 J
1–522（522 aa）
链 L
1–522（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CFN FE(7)-MO-S(9)-N CLUSTER × 2 CA CALCIUM ION × 2 CLF FE(8)-S(7) CLUSTER × 2 MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 4 SF4 IRON/SULFUR CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；PEG8000, Sodium Chloride, Magnesium Chloride, ADP, Dithionite, TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298 K
|
分辨率 3.10 Å R-free 0.270 |
| 2MIN NITROGENASE MOFE PROTEIN FROM AZOTOBACTER VINELANDII, OXIDIZED STATE 提交 1996-12-20 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–522（522 aa）
链 D
1–522（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CLF FE(8)-S(7) CLUSTER × 2 CFM FE-MO-S CLUSTER × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；PROTEIN WAS CRYSTALLIZED USING PRECIPITATING SOLUTION OF 30% PEG 4000, 0.2M NA2MO4, 0.1M TRIS PH 8.5
|
分辨率 2.03 Å R-free 0.266 |
| 3K1A Insights into substrate binding at FeMo-cofactor in nitrogenase from the structure of an alpha-70Ile MoFe protein variant 提交 2009-09-26 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
2–523（522 aa）
链 D
2–523（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CFN FE(7)-MO-S(9)-N CLUSTER × 2 CLF FE(8)-S(7) CLUSTER × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 8；298 K；30% PEG 4000, 100mM Tris-HCl pH 8.0, 170-190mM Sodium molybdate and 1mM Dithionite, LIQUID DIFFUSION, temperature 298.0K
|
分辨率 2.23 Å R-free 0.254 |
| 3MIN NITROGENASE MOFE PROTEIN FROM AZOTOBACTER VINELANDII, OXIDIZED STATE 提交 1996-12-20 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–522（522 aa）
链 D
1–522（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CFM FE-MO-S CLUSTER × 2 CA CALCIUM ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；PROTEIN WAS CRYSTALLIZED USING PRECIPITATING SOLUTION OF 30% PEG 4000, 0.2M NA2MO4, 0.1M TRIS PH 8.5 SOAKED IN SOAKED IN SODIUM DITHIONITE, 10MM FINAL CONC.
|
分辨率 2.03 Å R-free 0.264 |
| 3U7Q A. vinelandii nitrogenase MoFe protein at atomic resolution 提交 2011-10-14 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 IMD IMIDAZOLE × 8 CA CALCIUM ION × 2 1CL FE(8)-S(7) CLUSTER, OXIDIZED × 2 CLF FE(8)-S(7) CLUSTER × 2 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；291 K；16.5% v/v PEG6000, 0.55 M sodium chloride, 12.5% v/v MPD, 1.5% v/v xylitol, 0.2 M imidazole/malate buffer, pH 8.0, 0.1 mM Zwittergent 3-14, 0.55 mM spermine, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
分辨率 1.00 Å R-free 0.146 |
| 4ND8 Av Nitrogenase MoFe Protein High pH Form 提交 2013-10-25 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 1CL FE(8)-S(7) CLUSTER, OXIDIZED × 2 FE FE (III) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 9.79；295 K；150 mM ACES, 75 mM Tris and 75 mM ethanolamine, pH 9.79, 17%-18% PEG 3350, 0.8 M NaCl, 1 mM sodium dithionite, VAPOR DIFFUSION, temperature 295.K
|
分辨率 2.00 Å R-free 0.204 |
| 4TKU Reactivated Nitrogenase MoFe-protein from A. vinelandii 提交 2014-05-27 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 IMD IMIDAZOLE × 8 CL CHLORIDE ION × 2 CLF FE(8)-S(7) CLUSTER × 2 FE2 FE (II) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；294 K；PEG4000, NaCl, imidazole/malate, glycerol, sodium dithionite
|
分辨率 1.43 Å R-free 0.142 |
| 4TKV CO-bound Nitrogenase MoFe-protein from A. vinelandii 提交 2014-05-28 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICE iron-sulfur-molybdenum cluster with interstitial carbon × 4 IMD IMIDAZOLE × 8 CMO CARBON MONOXIDE × 6 CLF FE(8)-S(7) CLUSTER × 2 FE2 FE (II) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；294 K；PEG4000, NaCl, imidazole/malate, Glycerol, sodium dithionite
|
分辨率 1.50 Å R-free 0.154 |
| 4WNA Structure of the Nitrogenase MoFe Protein from Azotobacter vinelandii Pressurized with Xenon 提交 2014-10-11 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 XE XENON × 6 FE FE (III) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；297 K；PEG 6000, sodium chloride, xylitol, imidazole/malate, spermine, Zwittergent
|
分辨率 2.00 Å R-free 0.219 |
| 4WZA Asymmetric Nucleotide Binding in the Nitrogenase Complex 提交 2014-11-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 B
2–523（522 aa）
链 D
2–523（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；18-22% PEG 8000, 0-50 mM NaCl, 100 mM Tris pH 8.5, 10 mM sodium dithionite, 10 mM MgCl2, 5 mM AMPPCP, 5 mM ADP
|
分辨率 1.90 Å R-free 0.187 |
| 4WZB Crystal Structure of MgAMPPCP-bound Av2-Av1 complex 提交 2014-11-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 B
2–523（522 aa）
链 D
2–523（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 FE2 FE (II) ION × 2 MG MAGNESIUM ION × 4 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 SF4 IRON/SULFUR CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；PEG 8000, TRIS, SODIUM CHLORIDE, MAGNESIUM CHLORIDE, DITHIONITE, AMPPCP, PH 8.5
|
分辨率 2.30 Å R-free 0.255 |
| 4XPI Fe protein independent substrate reduction by nitrogenase variants altered in intramolecular electron transfer 提交 2015-01-17 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
2–523（522 aa）
链 D
2–523（522 aa）
|
突变：Y98H 突变：Y98H | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 GOL GLYCEROL × 15 1CL FE(8)-S(7) CLUSTER, OXIDIZED × 2 CA CALCIUM ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 8；298 K；30% PEG 4000, 100mM Tris-HCl pH 8.0, 170-190mM Sodium molybdate and 1mM Dithionite, LIQUID DIFFUSION, temperature 298.0K
|
分辨率 1.97 Å R-free 0.264 |
| 5BVG Selenium incorporated nitrogenase MoFe-protein (Av1-Se2B) from A. vinelandii 提交 2015-06-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICG iron-sulfur-molybdenum cluster with interstitial carbon with selenium incorporated × 2 IMD IMIDAZOLE × 9 CL CHLORIDE ION × 2 CLF FE(8)-S(7) CLUSTER × 2 FE2 FE (II) ION × 2 SE SELENIUM ATOM × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；291 K；PEG 8000, NaCl, Imidazole/Malate, Sodiumdithionite
|
分辨率 1.60 Å R-free 0.179 |
| 5BVH CO-bound form of Selenium incorporated nitrogenase MoFe-protein (Av1-Se-CO) from A. vinelandii 提交 2015-06-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 ICH iron-sulfur-molybdenum cluster with interstitial carbon with selenium incorporated × 2 IMD IMIDAZOLE × 8 CL CHLORIDE ION × 2 CMO CARBON MONOXIDE × 2 CLF FE(8)-S(7) CLUSTER × 2 FE2 FE (II) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；291 K；PEG 8000, NaCl, Imidazole/Malate, Sodiumdithionite
|
分辨率 1.53 Å R-free 0.160 |
| 5CX1 Nitrogenase molybdenum-iron protein beta-K400E mutant 提交 2015-07-28 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
突变：K400D 突变：K400D | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris pH 8, 600 mM NaCl, 18% PEG 10000, 5 mM sodium dithionite
|
分辨率 1.75 Å R-free 0.245 |
| 5CX1 Nitrogenase molybdenum-iron protein beta-K400E mutant 提交 2015-07-28 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 F
1–523（523 aa）
链 H
1–523（523 aa）
|
突变：K400D 突变：K400D | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris pH 8, 600 mM NaCl, 18% PEG 10000, 5 mM sodium dithionite
|
分辨率 1.75 Å R-free 0.245 |
| 5CX1 Nitrogenase molybdenum-iron protein beta-K400E mutant 提交 2015-07-28 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 J
1–523（523 aa）
链 L
1–523（523 aa）
|
突变：K400D 突变：K400D | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris pH 8, 600 mM NaCl, 18% PEG 10000, 5 mM sodium dithionite
|
分辨率 1.75 Å R-free 0.245 |
| 5CX1 Nitrogenase molybdenum-iron protein beta-K400E mutant 提交 2015-07-28 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 N
1–523（523 aa）
链 P
1–523（523 aa）
|
突变：K400D 突变：K400D | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris pH 8, 600 mM NaCl, 18% PEG 10000, 5 mM sodium dithionite
|
分辨率 1.75 Å R-free 0.245 |
| 5VQ4 Nitrogenase Av1 at pH 5 提交 2017-05-07 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 4000, sodium chloride, imidazole/malate, sodium dithionite
|
分辨率 2.30 Å R-free 0.222 |
| 6BBL Crystal structure of the a-96Gln MoFe protein variant in the presence of the substrate acetylene 提交 2017-10-18 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | C2H acetylene × 2 IMD IMIDAZOLE × 3 HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 1CL FE(8)-S(7) CLUSTER, OXIDIZED × 2 FE FE (III) ION × 2 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
MICROBATCH；pH 8；295 K；30% w/v PEG4000, 0.1 M Tris, pH 8.0, 0.17 M sodium molybdate, 0.001 M sodium dithionite
|
分辨率 1.68 Å R-free 0.185 |
| 6CDK Characterization of the P1+ intermediate state of nitrogenase P-cluster 提交 2018-02-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 8；298 K；30% PEG4000, 100 mM Tris-HCl, pH 8.0, 170-190 mM sodium molybdate, 1 mM dithionite
|
分辨率 2.10 Å R-free 0.263 |
| 6O7L Nitrogenase MoFeP mutant S188A from Azotobacter vinelandii in the dithionite reduced state after redox cycling 提交 2019-03-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
突变：S188A 突变：S188A | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；18% PEG 8000, 100 mM Tris pH 7.5, 500 mM NaCl, 10 mM dithionite
|
分辨率 2.26 Å R-free 0.254 |
| 6O7M Nitrogenase MoFeP mutant F99Y from Azotobacter vinelandii in the indigo carmine oxidized state 提交 2019-03-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
突变：F99Y 突变：F99Y | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.3；293 K；18% PEG 8000, 100 mM Tris pH 8.3, 500 mM NaCl, 5 mM indigo carmine (IDS)
|
分辨率 1.40 Å R-free 0.208 |
| 6O7N Nitrogenase MoFeP mutant F99Y/S188A from Azotobacter vinelandii in the indigo carmine oxidized state 提交 2019-03-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
突变：F99Y, S188A 突变：F99Y, S188A | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.8；293 K；18% PEG 8000, 100 mM Tris pH 7.8, 500 mM NaCl, 5 mM indigo carmine
|
分辨率 1.75 Å R-free 0.223 |
| 6O7O Nitrogenase MoFeP mutant F99Y/S188A from Azotobacter vinelandii in the dithionite reduced state after redox cycling 提交 2019-03-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
突变：F99Y, S188A 突变：F99Y, S188A | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；18% PEG 8000, 100 mM Tris pH 8.5, 500 mM NaCl, 10 mM dithionite. Protein was redox cycled with 5 mM indigo carmine and 10 mM dithionite prior to crystallization.
|
分辨率 1.89 Å R-free 0.186 |
| 6O7P Nitrogenase MoFeP mutant F99Y from Azotobacter vinelandii in the dithionite reduced state 提交 2019-03-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
突变：F99Y 突变：F99Y | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.3；293 K；22% PEG 8000, 100 mM Tris pH 8.3, 500 mM NaCl, 10 mM dithionite
|
分辨率 1.70 Å R-free 0.201 |
| 6O7Q Nitrogenase MoFeP mutant S188A from Azotobacter vinelandii in the dithionite reduced state after redox cycling 提交 2019-03-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
突变：S188A 突变：S188A | CLF FE(8)-S(7) CLUSTER × 2 HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；20% PEG 8000, 100 mM Tris pH 8.0, 500 mM NaCl, 10 mM dithionite. Protein was redox cycled with 5 mM indigo carmine and 10 mM dithionite prior to crystallization.
|
分辨率 2.00 Å R-free 0.229 |
| 6O7R Nitrogenase MoFeP mutant F99Y, S188A from Azotobacter vinelandii in the dithionite reduced state 提交 2019-03-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
突变：F99Y, S188A 突变：F99Y, S188A | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；20% PEG 8000, 100 mM Tris pH 8.0, 500 mM NaCl, 10 mM dithionite
|
分辨率 2.27 Å R-free 0.208 |
| 6O7S Nitrogenase MoFeP mutant S188A from Azotobacter vinelandii in the indigo carmine oxidized state 提交 2019-03-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
突变：S188A 突变：S188A | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 NA SODIUM ION × 2 LPJ FE(6)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；20% PEG 8000, 100 mM Tris pH 8.0, 500 mM NaCl
|
分辨率 2.27 Å R-free 0.208 |
| 6OP1 Selenium incorporated, carbon monoxide inhibited FeMo-cofactor of azotobacter vinelandii 提交 2019-04-24 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
2–523（522 aa）
链 D
2–523（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 IMD IMIDAZOLE × 9 CMO CARBON MONOXIDE × 2 MG MAGNESIUM ION × 5 CLF FE(8)-S(7) CLUSTER × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；291 K；26% v/v PEG6000, 0.75 M sodium chloride, 12.5% v/v MPD, 0.1 M imidazole/malate buffer, pH 8.0, 5 mM Na2S2O4
|
分辨率 1.70 Å R-free 0.165 |
| 6OP2 Selenium incorporated FeMo-cofactor of nitrogenase from azotobacter vinelandii at high concentration of selenium 提交 2019-04-24 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
2–523（522 aa）
链 D
2–523（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 IMD IMIDAZOLE × 11 SE SELENIUM ATOM × 2 CA CALCIUM ION × 2 CLF FE(8)-S(7) CLUSTER × 2 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；291 K；26% v/v PEG6000, 0.75 M sodium chloride, 12.5% v/v MPD, 0.1 M imidazole/malate buffer, pH 8.0, 5 mM Na2S2O4
|
分辨率 1.90 Å R-free 0.214 |
| 6OP3 Selenium incorporated FeMo-cofactor of nitrogenase from Azotobacter vinelandii with low concentration of selenium 提交 2019-04-24 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
2–523（522 aa）
链 D
2–523（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 IMD IMIDAZOLE × 12 SE SELENIUM ATOM × 2 CLF FE(8)-S(7) CLUSTER × 2 MG MAGNESIUM ION × 2 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；291 K；26% v/v PEG6000, 0.75 M sodium chloride, 12.5% v/v MPD, 0.1 M imidazole/malate buffer, pH 8.0, 5 mM Na2S2O4
|
分辨率 1.60 Å R-free 0.152 |
| 6OP4 Selenium-incorporated, carbon monoxide-inhibited, reactivated FeMo-cofactor of nitrogenase from Azotobacter vinelandii 提交 2019-04-24 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
2–523（522 aa）
链 D
2–523（522 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 IMD IMIDAZOLE × 9 CLF FE(8)-S(7) CLUSTER × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；291 K；26% v/v PEG6000, 0.75 M sodium chloride, 12.5% v/v MPD, 0.1 M imidazole/malate buffer, pH 8.0, 5 mM Na2S2O4
|
分辨率 2.30 Å R-free 0.223 |
| 6UG0 N2-bound Nitrogenase MoFe-protein from Azotobacter vinelandii 提交 2019-09-25 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICE iron-sulfur-molybdenum cluster with interstitial carbon × 1 HDZ NITROGEN MOLECULE × 3 H2S HYDROSULFURIC ACID × 6 MO MOLYBDENUM ATOM × 8 1CL FE(8)-S(7) CLUSTER, OXIDIZED × 2 FE FE (III) ION × 2 GOL GLYCEROL × 6 PGE TRIETHYLENE GLYCOL × 1 ICZ iron-sulfur-molybdenum cluster with interstitial carbon × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；14-19 % w/v PEG smear high, 0.16M ammonium acetate, 0.1M sodium citrate (pH 5.0), 25.6 % v/v glycerol, 5mM Eu(II)-EGTA
|
分辨率 1.83 Å R-free 0.259 |
| 6VXT Activated Nitrogenase MoFe-protein from Azotobacter vinelandii 提交 2020-02-24 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 MO MOLYBDENUM ATOM × 8 H2S HYDROSULFURIC ACID × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 GOL GLYCEROL × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；14-19 % w/v PEG smear high, 0.16 M ammonium acetate, 0.1 M sodium citrate(pH 5.0), 25.6 % v/v glycerol, 5 mM sodium dithionite
|
分辨率 1.74 Å R-free 0.203 |
| 7JRF CO-CO-BOUND NITROGENASE MOFE-PROTEIN FROM A. VINELANDII 提交 2020-08-12 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICE iron-sulfur-molybdenum cluster with interstitial carbon × 2 IMD IMIDAZOLE × 8 H2S HYDROSULFURIC ACID × 2 CMO CARBON MONOXIDE × 4 CLF FE(8)-S(7) CLUSTER × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；Peg 4000, sodium chloride, imidazole / malate, glycerol, spermine
|
分辨率 1.33 Å R-free 0.150 |
| 8BTS Nitrogenase MoFe protein from A. vinelandii alpha double mutant C45A/L158C 提交 2022-11-29 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 GOL GLYCEROL × 10 SO4 SULFATE ION × 5 1CL FE(8)-S(7) CLUSTER, OXIDIZED × 2 FE FE (III) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5.5；293.15 K；The purified enzyme in 50 mM Tris/HCl pH 8.0, 300 mM NaCl, 2 mM dithionite was crystallized at 8 mg.ml-1. Crystallization was performed anaerobically in a tent containing 100 % N2 atmosphere. Crystals were obtained by initial screening at 20 degree Celsius on 96-Well MRC 2-drop crystallization plates in polystyrene (SWISSCI) containing 90 ul of crystallization solution in the reservoir. The protein sample (0.5 ul) was mixed with 0.5 ul reservoir solution. The reservoir solution contained 25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS at pH 5.5, and 200 mM Lithium sulfate. The plate was then incubated in a Coy tent (N2/H2, 97:3) at 20 degree Celsius. Crystals appeared after a few weeks and were soaked in the crystallization solution supplemented with 15 % v/v glycerol as a cryo-protectant before being frozen in liquid nitrogen.
|
分辨率 3.03 Å R-free 0.223 |
| 8BTS Nitrogenase MoFe protein from A. vinelandii alpha double mutant C45A/L158C 提交 2022-11-29 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 I
1–523（523 aa）
链 L
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 GOL GLYCEROL × 2 SO4 SULFATE ION × 5 1CL FE(8)-S(7) CLUSTER, OXIDIZED × 2 FE FE (III) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5.5；293.15 K；The purified enzyme in 50 mM Tris/HCl pH 8.0, 300 mM NaCl, 2 mM dithionite was crystallized at 8 mg.ml-1. Crystallization was performed anaerobically in a tent containing 100 % N2 atmosphere. Crystals were obtained by initial screening at 20 degree Celsius on 96-Well MRC 2-drop crystallization plates in polystyrene (SWISSCI) containing 90 ul of crystallization solution in the reservoir. The protein sample (0.5 ul) was mixed with 0.5 ul reservoir solution. The reservoir solution contained 25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS at pH 5.5, and 200 mM Lithium sulfate. The plate was then incubated in a Coy tent (N2/H2, 97:3) at 20 degree Celsius. Crystals appeared after a few weeks and were soaked in the crystallization solution supplemented with 15 % v/v glycerol as a cryo-protectant before being frozen in liquid nitrogen.
|
分辨率 3.03 Å R-free 0.223 |
| 8CRS CryoEM Structure of nitrogenase MoFe-protein in detergent 提交 2022-05-11 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
2–523（522 aa）
链 D
2–523（522 aa）
|
未记录 | ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 1N7 CHAPSO × 4 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 2.04 Å |
| 8DBX CryoEM structure of partially oxidized MoFe-protein on ultrathin carbon 提交 2022-06-14 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CLF FE(8)-S(7) CLUSTER × 2 1CL FE(8)-S(7) CLUSTER, OXIDIZED × 2 FE FE (III) ION × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 1.92 Å |
| 8DBY CryoEM structure of anaerobically prepared nitrogenase MoFe-protein on ultrathin carbon 提交 2022-06-15 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 2.26 Å |
| 8DFC CryoEM structure of the 1:1 ADP-tetrafluoroaluminate stabilized nitrogenase complex from Azotobacter vinelandii 提交 2022-06-21 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 SF4 IRON/SULFUR CLUSTER × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ALF TETRAFLUOROALUMINATE ION × 2 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 2.48 Å |
| 8DFD CryoEM structure of the 2:1 ADP-tetrafluoroaluminate stabilized nitrogenase complex from Azotobacter vinelandii 提交 2022-06-21 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 4 ALF TETRAFLUOROALUMINATE ION × 4 MG MAGNESIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 2.12 Å |
| 8ENL CryoEM structure of the high pH turnover-inactivated nitrogenase MoFe-protein 提交 2022-09-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
2–523（522 aa）
链 D
2–523（522 aa）
|
未记录 | ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 UNX UNKNOWN LIGAND × 2 CLF FE(8)-S(7) CLUSTER × 2 1N7 CHAPSO × 2 FE FE (III) ION × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 2.37 Å |
| 8ENM CryoEM structure of the high pH nitrogenase MoFe-protein under non-turnover conditions 提交 2022-09-30 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 2.14 Å |
| 8RHP Cryo-EM structure of the molybdenum nitrogenase complexed with iron protein (NifH) and Shethna protein II (FeSII) 提交 2023-12-15 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 14 PDB 声明：14-meric（14） 与蛋白数一致 |
链 B
1–523（523 aa）
链 C
1–523（523 aa）
链 I
1–523（523 aa）
链 J
1–523（523 aa）
|
未记录 | CLF FE(8)-S(7) CLUSTER × 4 HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 4 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 4 CA CALCIUM ION × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.89 Å |
| 9CJB CryoEM structure of nitrogenase MoFe-protein 60 minute time point under alkaline turnover 提交 2024-07-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 9.5
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 1.97 Å |
| 9CJC CryoEM structure of nitrogenase MoFe-protein 20 minute time point under alkaline turnover 提交 2024-07-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 1 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 9.5
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 2.04 Å |
| 9CJD CryoEM structure of nitrogenase MoFe-protein 5 minute time point under alkaline turnover 提交 2024-07-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 1 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 9.5
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 1.92 Å |
| 9CJE CryoEM structure of nitrogenase MoFe-protein 20 second time point under alkaline turnover 提交 2024-07-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 1 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 9.5
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 2.22 Å |
| 9CJF CryoEM structure of alkaline-inactivated nitrogenase MoFe-protein in complex with NafT 提交 2024-07-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 5 PDB 声明：pentameric（5） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 1N7 CHAPSO × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 2.33 Å |
| 9CQX Azotobacter vinelandii Oxidized MoFeP (C1 symmetry) obtained using the SPT Labtech chameleon 提交 2024-07-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.51 Å |
| 9CQY Azotobacter vinelandii Oxidized MoFeP (C2 symmetry) obtained using the SPT Labtech chameleon 提交 2024-07-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.39 Å |
| 9CQZ Azotobacter vinelandii Reduced MoFeP (C1 symmetry) obtained using the SPT Labtech chameleon of 20 mM sodium dithionite under Al's oil 提交 2024-07-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.19 Å |
| 9CR0 Azotobacter vinelandii Reduced MoFeP (C2 symmetry) obtained using the SPT Labtech chameleon of 20 mM sodium dithionite under Al's oil 提交 2024-07-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.08 Å |
| 9CTZ Azotobacter vinelandii MoFeP (C2 symmetry) 提交 2024-07-25 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.67 Å |
| 9CU0 Azotobacter vinelandii 1:1:1 MoFeP:FeP:FeSII-Complex (C1 symmetry) 提交 2024-07-25 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 7 PDB 声明：heptameric（7） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 FE FE (III) ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 MG MAGNESIUM ION × 2 SF4 IRON/SULFUR CLUSTER × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 3.94 Å |
| 9CU1 Azotobacter vinelandii filamentous 2:2:1 MoFeP:FeP:FeSII-Complex (termini; C1 symmetry) 提交 2024-07-25 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 14 PDB 声明：14-meric（14） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
链 I
1–523（523 aa）
链 K
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 4 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 4 CLF FE(8)-S(7) CLUSTER × 4 FE FE (III) ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 SF4 IRON/SULFUR CLUSTER × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.83 Å |
| 9CU2 Azotobacter vinelandii filamentous 2:2:1 MoFeP:FeP:FeSII-Complex (C2 symmetry) 提交 2024-07-25 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 14 PDB 声明：14-meric（14） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
链 I
1–523（523 aa）
链 K
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 4 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 4 FE FE (III) ION × 4 CLF FE(8)-S(7) CLUSTER × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 SF4 IRON/SULFUR CLUSTER × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.27 Å |
| 9HB9 A. vinelandii nitrogenase MoFe protein Anc1a 提交 2024-11-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 CLF FE(8)-S(7) CLUSTER × 2 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.2 M LiSO4 x 1 H2O, 0.1 M bis-Tris/HCl at pH 6.5, 25%(w/v) of polyethylene glycol 3350
|
分辨率 2.66 Å R-free 0.220 |
| 9HBC A. vinelandii nitrogenase MoFe protein Anc2 提交 2024-11-06 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 CLF FE(8)-S(7) CLUSTER × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 EDO 1,2-ETHANEDIOL × 2 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；4.5% (w/v) of polyethylene glycol 2000, 3350, 4000 and polyethylene glycol mono methylether 5000, 0.1 M MES/NaOH at pH 6.5, 10% (v/v) of ethylene glycol and 0.15 M Mg acetate
|
分辨率 1.82 Å R-free 0.225 |
| 9MLY Azotobacter vinelandii Reduced MoFeP (C1 symmetry) obtained using the SPT Labtech chameleon of 5 mM sodium dithionite under Al's oil 提交 2024-12-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.33 Å |
| 9MLZ Azotobacter vinelandii Reduced MoFeP (C2 symmetry) obtained using the SPT Labtech chameleon of 5 mM sodium dithionite under Al's oil 提交 2024-12-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.19 Å |
| 9MM0 Azotobacter vinelandii Reduced MoFeP (C1 symmetry) obtained using the SPT Labtech chameleon of 60 mM sodium dithionite under Al's oil 提交 2024-12-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.19 Å |
| 9MM1 Azotobacter vinelandii Reduced MoFeP (C2 symmetry) obtained using the SPT Labtech chameleon of 60 mM sodium dithionite under Al's oil 提交 2024-12-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–523（523 aa）
链 D
1–523（523 aa）
|
未记录 | HCA 3-HYDROXY-3-CARBOXY-ADIPIC ACID × 2 ICS iron-sulfur-molybdenum cluster with interstitial carbon × 2 FE FE (III) ION × 2 CLF FE(8)-S(7) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Samples were frozen with the SPT Labtech chameleon
|
分辨率 2.08 Å |