Current Protein Identity：P38501
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1AQ8 STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE REDUCED WITH ASCORBATE Deposited 1997-08-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
34–376（343 aa）
Chain B
34–376（343 aa）
Chain C
34–376（343 aa）
|
Not recorded | CU COPPER (II) ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 4.5；10% PEG4000, 0.1 SODIUM ACETATE PH 4.5
|
Resolution 2.00 Å |
| 1AS6 STRUCTURE OF NITRITE BOUND TO OXIDIZED ALCALIGENES FAECALIS NITRITE REDUCTASE AT CRYO TEMPERATURE Deposited 1997-08-13 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
34–376（343 aa）
Chain B
34–376（343 aa）
Chain C
34–376（343 aa）
|
Not recorded | CU COPPER (II) ION × 6 NO2 NITRITE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 4.5；10% PEG4000, 0.1 SODIUM ACETATE PH 4.5
|
Resolution 1.80 Å |
| 1AS7 STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE AT CRYO TEMPERATURE Deposited 1997-08-13 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
34–376（343 aa）
Chain B
34–376（343 aa）
Chain C
34–376（343 aa）
|
Not recorded | CU COPPER (II) ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 4.5；10% PEG4000, 0.1 SODIUM ACETATE PH 4.5
|
Resolution 2.00 Å |
| 1AS8 STRUCTURE OF NITRITE BOUND TO REDUCED ALCALIGENES FAECALIS NITRITE REDUCTASE AT CRYO TEMPERATURE Deposited 1997-08-13 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
34–376（343 aa）
Chain B
34–376（343 aa）
Chain C
34–376（343 aa）
|
Not recorded | CU COPPER (II) ION × 6 NO2 NITRITE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 4.5；10% PEG4000, 0.1 SODIUM ACETATE PH 4.5
|
Resolution 1.85 Å |
| 1ET5 CRYSTAL STRUCTURE OF NITRITE REDUCTASE ASP98ASN MUTANT FROM ALCALIGENES FAECALIS S-6 Deposited 2000-04-12 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：40 - 376
|
Mutation：D98N | CU COPPER (II) ION × 6 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.7；298 K；sodium cacodylate, zinc acetate, PEG 4000, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.90 Å R-free 0.214 |
| 1ET7 CRYSTAL STRUCTURE OF NITRITE REDUCTASE HIS255ASP MUTANT FROM ALCALIGENES FAECALIS S-6 Deposited 2000-04-12 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：40 - 376
|
Mutation：H255D | CU COPPER (II) ION × 6 CD CADMIUM ION × 9 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.7；298 K；PEG 6000 sodium cacodylate, cadmium acetate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.70 Å R-free 0.210 |
| 1ET8 CRYSTAL STRUCTURE OF NITRITE REDUCTASE HIS255ASN MUTANT FROM ALCALIGENES FAECALIS Deposited 2000-04-12 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：40 - 376
|
Mutation：H255N | CU COPPER (II) ION × 6 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.7；298 K；PEG 8000, sodium cacodylate, zinc acetate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.80 Å R-free 0.212 |
| 1J9Q Crystal structure of nitrite soaked oxidized D98N AFNIR Deposited 2001-05-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：Residues 40-376
Chain B
40–376（337 aa）
Fragment：Residues 40-376
Chain C
40–376（337 aa）
Fragment：Residues 40-376
|
Mutation：D98N Mutation：D98N Mutation：D98N | CU COPPER (II) ION × 6 NO2 NITRITE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；PEG 6000, sodium acetate, sodium nitrite, copper chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.65 Å R-free 0.210 |
| 1J9R Crystal structure of nitrite soaked reduced D98N AFNIR Deposited 2001-05-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain B
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain C
40–376（337 aa）
Fragment：RESIDUES 40-376
|
Mutation：D98N Mutation：D98N Mutation：D98N | CU COPPER (II) ION × 9 NO2 NITRITE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；PEG 6000, sodium acetate, sodium nitrite, copper chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.225 |
| 1J9S Crystal structure of nitrite soaked oxidized H255N AFNIR Deposited 2001-05-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain B
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain C
40–376（337 aa）
Fragment：RESIDUES 40-376
|
Mutation：H255N Mutation：H255N Mutation：H255N | CU COPPER (II) ION × 6 NO2 NITRITE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；PEG 6000, sodium acetate, copper chloride, sodium nitrite, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.204 |
| 1J9T Crystal structure of nitrite soaked reduced H255N AFNIR Deposited 2001-05-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain B
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain C
40–376（337 aa）
Fragment：RESIDUES 40-376
|
Mutation：H255N Mutation：H255N Mutation：H255N | CU COPPER (II) ION × 9 NO2 NITRITE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；PEG 6000, sodium acetate, sodium nitrite, copper chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.95 Å R-free 0.218 |
| 1L9O CRYSTAL STRUCTURE OF NITRITE SOAKED I257A VARIANT OF THE COPPER-CONTAINING NITRITE REDUCTASE FROM ALCALIGENES FAECALIS Deposited 2002-03-26 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain B
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain C
40–376（337 aa）
Fragment：RESIDUES 40-376
|
Mutation：I257A Mutation：I257A Mutation：I257A | CU COPPER (II) ION × 6 NO2 NITRITE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；sodium acetate peg 4000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.192 |
| 1L9P CRYSTAL STRUCTURE OF NITRITE SOAKED I257G VARIANT OF THE COPPER-CONTAINING NITRITE REDUCTASE FROM ALCALIGENES FAECALIES S-6 Deposited 2002-03-26 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain B
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain C
40–376（337 aa）
Fragment：RESIDUES 40-376
|
Mutation：I257G Mutation：I257G Mutation：I257G | CU COPPER (II) ION × 6 NO2 NITRITE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；acetate peg 4000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.75 Å R-free 0.194 |
| 1L9Q CRYSTAL STRUCTURE OF THE I257L VARIANT OF THE COPPER-CONTAINING NITRITE REDUCTASE FROM ALCALIGENES FAECALIS S-6 Deposited 2002-03-26 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain B
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain C
40–376（337 aa）
Fragment：RESIDUES 40-376
|
Mutation：I257L Mutation：I257L Mutation：I257L | CU COPPER (II) ION × 6 NO2 NITRITE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；acetate peg 4000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.188 |
| 1L9R CRYSTAL STRUCTURE OF THE I257M VARIANT OF THE COPPER-CONTAINING NITRITE REDUCTASE FROM ALCALIGENES FAECALIS S-6 Deposited 2002-03-26 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain B
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain C
40–376（337 aa）
Fragment：RESIDUES 40-376
|
Mutation：I257M Mutation：I257M Mutation：I257M | CU COPPER (II) ION × 6 NO2 NITRITE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；acetate peg 4000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.78 Å R-free 0.197 |
| 1L9S CRYSTAL STRUCTURE OF THE I257T VARIANT OF THE COPPER-CONTAINING NITRITE REDUCTASE FROM ALCALIGENES FAECALIS S-6 Deposited 2002-03-26 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain B
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain C
40–376（337 aa）
Fragment：RESIDUES 40-376
|
Mutation：I257T Mutation：I257T Mutation：I257T | CU COPPER (II) ION × 6 NO2 NITRITE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；acetate peg 4000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.78 Å R-free 0.197 |
| 1L9T CRYSTAL STRUCTURE OF THE I257V VARIANT OF THE COPPER-CONTAINING NITRITE REDUCTASE FROM ALCALIGENES FAECALIS S-6 Deposited 2002-03-26 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain B
40–376（337 aa）
Fragment：RESIDUES 40-376
Chain C
40–376（337 aa）
Fragment：RESIDUES 40-376
|
Mutation：I257V Mutation：I257V Mutation：I257V | CU COPPER (II) ION × 6 NO2 NITRITE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；acetate peg 4000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.75 Å R-free 0.208 |
| 1NPJ Crystal structure of H145A mutant of nitrite reductase from Alcaligenes faecalis Deposited 2003-01-18 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
34–376（343 aa）
Chain B
34–376（343 aa）
Chain C
34–376（343 aa）
|
Mutation：H145A Mutation：H145A Mutation：H145A | CU COPPER (II) ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；300 K；PEG 8000, sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 1.90 Å R-free 0.225 |
| 1NPN Crystal structure of a copper reconstituted H145A mutant of nitrite reductase from Alcaligenes faecalis Deposited 2003-01-18 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
34–376（343 aa）
Chain B
34–376（343 aa）
Chain C
34–376（343 aa）
|
Mutation：H145A Mutation：H145A Mutation：H145A | CU COPPER (II) ION × 6 CL CHLORIDE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；300 K；PEG 8000, sodium acetate , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 1.80 Å R-free 0.195 |
| 1NTD STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE MUTANT M150E THAT CONTAINS ZINC Deposited 1995-07-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
34–376（343 aa）
|
Mutation：M150E | CU COPPER (II) ION × 6 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.30 Å R-free 0.232 |
| 1SJM Nitrite bound copper containing nitrite reductase Deposited 2004-03-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Chain B
40–376（337 aa）
Chain C
40–376（337 aa）
|
Not recorded | CU COPPER (II) ION × 6 NO2 NITRITE ION × 3 ACT ACETATE ION × 5 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；PEG 4000, sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 321K
|
Resolution 1.40 Å R-free 0.138 |
| 1SNR Nitric oxide bound to Cu nitrite reductase Deposited 2004-03-11 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Chain B
40–376（337 aa）
Chain C
40–376（337 aa）
|
Not recorded | CU1 COPPER (I) ION × 3 CU COPPER (II) ION × 3 ACT ACETATE ION × 8 NO NITRIC OXIDE × 3 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；291 K；PEG4000, sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 4.0
|
Resolution 1.31 Å R-free 0.141 |
| 1ZDQ Crystal Structure of Met150Gly AfNiR with Methylsulfanyl Methane Bound Deposited 2005-04-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–375（336 aa）
Chain B
40–375（336 aa）
Chain C
40–375（336 aa）
|
Mutation：M150G Mutation：M150G Mutation：M150G | CU COPPER (II) ION × 6 MSM (METHYLSULFANYL)METHANE × 3 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.80 Å R-free 0.199 |
| 1ZDS Crystal Structure of Met150Gly AfNiR with Acetamide Bound Deposited 2005-04-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–375（336 aa）
Chain B
40–375（336 aa）
Chain C
40–375（336 aa）
|
Mutation：M150G Mutation：M150G Mutation：M150G | CU COPPER (II) ION × 6 ACM ACETAMIDE × 6 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.55 Å R-free 0.214 |
| 2AFN STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE AND A COPPER SITE MUTANT, M150E, THAT CONTAINS ZINC Deposited 1995-07-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
34–376（343 aa）
Chain B
34–376（343 aa）
Chain C
34–376（343 aa）
|
Not recorded | CU COPPER (II) ION × 6 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.00 Å R-free 0.223 |
| 2B08 Reduced acetamide-bound M150G Nitrite Reductase from Alcaligenes faecalis Deposited 2005-09-13 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
37–376（340 aa）
Chain B
37–376（340 aa）
Chain C
37–376（340 aa）
|
Mutation：M150G Mutation：M150G Mutation：M150G | CU1 COPPER (I) ION × 9 ACM ACETAMIDE × 4 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.90 Å R-free 0.220 |
| 2E86 Azide bound to copper containing nitrite reductase from A. faecalis S-6 Deposited 2007-01-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：residues 4-340
Chain B
40–376（337 aa）
Fragment：residues 4-340
Chain C
40–376（337 aa）
Fragment：residues 4-340
|
Not recorded | CU1 COPPER (I) ION × 3 CU COPPER (II) ION × 3 ACT ACETATE ION × 10 AZI AZIDE ION × 3 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；6-8 % PEG 4000, 0.1M sodium acetate buffer pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.50 Å R-free 0.179 |
| 2FJS Crystal Structure of Anaerobically Reduced Wild Type Nitrite Reductase from A. faecalis Deposited 2006-01-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Chain B
40–376（337 aa）
Chain C
40–376（337 aa）
|
Not recorded | CU1 COPPER (I) ION × 3 CU COPPER (II) ION × 3 ACT ACETATE ION × 10 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；8-10% PEG 4000, 0.1 M NaOAC, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.85 Å R-free 0.180 |
| 2P80 Solution structure of the complex between nitrite reductase and pseudoazurin from A. faecalis Deposited 2007-03-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
40–376（337 aa）
Chain B
40–376（337 aa）
Chain C
40–376（337 aa）
|
Not recorded | CU COPPER (II) ION × 7 GD GADOLINIUM ATOM × 9 | SOLUTION NMR |
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 50 mM phosphate；Pressure 1
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 50 mM phosphate；Pressure 1
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 50 mM phosphate；Pressure 1
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 50 mM phosphate；Pressure 1
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 50 mM phosphate；Pressure 1
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 50 mM phosphate；Pressure 1
NMR sample composition
250 mM 15N, 2H pseudoazurin in complex with nitrite reductase -bound GdCLaNP at position 221/223 (0.38 eq), in 50 mM phosphate buffer, H2O, 6% (v/v) D2O | H2O, 6% (v/v) D2O
NMR sample composition
250 mM 15N, 2H pseudoazurin in complex with nitrite reductase -bound YCLaNP at position 221/223 (0.38 eq), in 50 mM phosphate buffer, H2O, 6% (v/v) D2O | H2O, 6% (v/v) D2O
NMR sample composition
250 mM 15N, 2H pseudoazurin in complex with nitrite reductase -bound GdCLaNP at position 234/236 (0.32 eq), in 50 mM phosphate buffer, H2O, 6% (v/v) D2O | H2O, 6% (v/v) D2O
NMR sample composition
250 mM 15N, 2H pseudoazurin in complex with nitrite reductase -bound YCLaNP at position 234/236 (0.32 eq), in 50 mM phosphate buffer, H2O, 6% (v/v) D2O | H2O, 6% (v/v) D2O
NMR sample composition
250 mM 15N, 2H pseudoazurin in complex with nitrite reductase -bound GdCLaNP at position 333/336 (0.53 eq), in 50 mM phosphate buffer, H2O, 6% (v/v) D2O | H2O, 6% (v/v) D2O
NMR sample composition
250 mM 15N, 2H pseudoazurin in complex with nitrite reductase -bound YCLaNP at position 333/336 (0.53 eq), in 50 mM phosphate buffer, H2O, 6% (v/v) D2O | H2O, 6% (v/v) D2O
|
Resolution not provided |
| 2PP7 Crystal structure of anaerobically manipulated wild type oxidized AfNiR (acetate bound) Deposited 2007-04-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Chain B
40–376（337 aa）
Chain C
40–376（337 aa）
|
Not recorded | CU1 COPPER (I) ION × 3 CU COPPER (II) ION × 3 ACT ACETATE ION × 13 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；6-11% PEG 4000, 100mM sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.65 Å R-free 0.180 |
| 2PP8 Formate bound to oxidized wild type AfNiR Deposited 2007-04-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Chain B
40–376（337 aa）
Chain C
40–376（337 aa）
|
Not recorded | CU1 COPPER (I) ION × 3 CU COPPER (II) ION × 3 ACT ACETATE ION × 8 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 FMT FORMIC ACID × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；6-11% PEG 4000, 100mM sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.50 Å R-free 0.201 |
| 2PP9 Nitrate bound wild type oxidized AfNiR Deposited 2007-04-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Chain B
40–376（337 aa）
Chain C
40–376（337 aa）
|
Not recorded | CU1 COPPER (I) ION × 3 CU COPPER (II) ION × 3 NO3 NITRATE ION × 3 ACT ACETATE ION × 8 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；6-11% PEG 4000, 100mM sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.195 |
| 2PPA Anaerobically manipulated wild type oxidized AfNiR bound to nitrous oxide Deposited 2007-04-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Chain B
40–376（337 aa）
Chain C
40–376（337 aa）
|
Not recorded | CU1 COPPER (I) ION × 3 CU COPPER (II) ION × 3 ACT ACETATE ION × 10 N2O NITROUS OXIDE × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；6-11% PEG 4000, 100mM sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.69 Å R-free 0.224 |
| 2PPC Oxidized wild type AfNiR exposed to NO (nitrite bound) Deposited 2007-04-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Chain B
40–376（337 aa）
Chain C
40–376（337 aa）
|
Not recorded | CU1 COPPER (I) ION × 3 CU COPPER (II) ION × 3 NO2 NITRITE ION × 3 ACT ACETATE ION × 10 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；6-11% PEG 4000, 100mM sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.58 Å R-free 0.182 |
| 2PPD Oxidized H145A mutant of AfNiR bound to nitric oxide Deposited 2007-04-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
34–376（343 aa）
Chain B
34–376（343 aa）
Chain C
34–376（343 aa）
|
Mutation：H145A Mutation：H145A Mutation：H145A | CU1 COPPER (I) ION × 3 CU COPPER (II) ION × 3 NO NITRIC OXIDE × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 ACT ACETATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；6-11% polyethylene glycol 6000, 0.1 M ammonium sulfate and 0.01 M sodium acetate buffer, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.212 |
| 2PPE Reduced H145A mutant of AfNiR exposed to NO Deposited 2007-04-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
34–376（343 aa）
Chain B
34–376（343 aa）
Chain C
34–376（343 aa）
|
Mutation：H145A Mutation：H145A Mutation：H145A | CU1 COPPER (I) ION × 3 CU COPPER (II) ION × 3 ACT ACETATE ION × 2 NO NITRIC OXIDE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；6-11% polyethylene glycol 6000, 0.1 M ammonium sulfate and 0.01 M sodium acetate buffer, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.75 Å R-free 0.196 |
| 2PPF Reduced mutant D98N of AfNiR exposed to nitric oxide Deposited 2007-04-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Chain B
40–376（337 aa）
Chain C
40–376（337 aa）
|
Mutation：D98N Mutation：D98N Mutation：D98N | CU1 COPPER (I) ION × 5 CU COPPER (II) ION × 3 ACT ACETATE ION × 8 NO NITRIC OXIDE × 4 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；7-12% polyethylene glycol 4000, 75 mM acetamide and 10 mM sodium acetate buffer, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.65 Å R-free 0.170 |
| 3H4F Met62Leu variant of nitrite reductase from Alcaligenes faeclis Deposited 2009-04-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–375（336 aa）
Chain B
40–375（336 aa）
Chain C
40–375（336 aa）
|
Mutation：Met62Leu Mutation：Met62Leu Mutation：Met62Leu | CU COPPER (II) ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4.5；298 K；pH 4.5, VAPOR DIFFUSION, temperature 298K
|
Resolution 2.10 Å R-free 0.227 |
| 3H4H Met94Thr/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis Deposited 2009-04-20 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
41–375（335 aa）
Chain B
41–375（335 aa）
Chain C
41–375（335 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.60 Å R-free 0.226 |
| 3H56 Met150Leu/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis Deposited 2009-04-21 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–375（336 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4.5；298 K；pH 4.5, VAPOR DIFFUSION, temperature 298K
|
Resolution 1.50 Å R-free 0.204 |
| 4YSC Completely oxidized structure of copper nitrite reductase from Alcaligenes faecalis Deposited 2015-03-17 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：UNP residues 40-376
Chain B
40–376（337 aa）
Fragment：UNP residues 40-376
Chain C
40–376（337 aa）
Fragment：UNP residues 40-376
|
Not recorded | CU COPPER (II) ION × 6 CL CHLORIDE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
BATCH MODE；293 K；100 mM sodium acetate pH 4.0, 10% PEG 4000
|
Resolution 2.03 Å R-free 0.203 |
| 4YSE High resolution synchrotron structure of copper nitrite reductase from Alcaligenes faecalis Deposited 2015-03-17 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：UNP residues 40-376
Chain B
40–376（337 aa）
Fragment：UNP residues 40-376
Chain C
40–376（337 aa）
Fragment：UNP residues 40-376
|
Not recorded | CU COPPER (II) ION × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 6 ACY ACETIC ACID × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；100mM sodium acetate, pH 4, 8% PEG4000
|
Resolution 1.20 Å R-free 0.168 |
| 5D4H High-resolution nitrite complex of a copper nitrite reductase determined by synchrotron radiation crystallography Deposited 2015-08-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：UNP residue 40-376
Chain B
40–376（337 aa）
Fragment：UNP residue 40-376
Chain C
40–376（337 aa）
Fragment：UNP residue 40-376
|
Not recorded | CU COPPER (II) ION × 6 NO2 NITRITE ION × 3 GOL GLYCEROL × 22 ACY ACETIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.1；293 K；100mM sodium acetate (pH 4.1) and 7% PEG 4000
|
Resolution 1.30 Å R-free 0.165 |
| 5D4I Intact nitrite complex of a copper nitrite reductase determined by serial femtosecond crystallography Deposited 2015-08-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：UNP residues 40-376
Chain B
40–376（337 aa）
Fragment：UNP residues 40-376
Chain C
40–376（337 aa）
Fragment：UNP residues 40-376
|
Not recorded | CU COPPER (II) ION × 6 NO2 NITRITE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
BATCH MODE；pH 4；293 K；100mM sodium acetate (pH 4.0) and 12% PEG 4000
|
Resolution 1.60 Å R-free 0.196 |
| 5D4J Chloride-bound form of a copper nitrite reductase from Alcaligenes faecals Deposited 2015-08-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Fragment：UNP residues 40-376
Chain B
40–376（337 aa）
Fragment：UNP residues 40-376
Chain C
40–376（337 aa）
Fragment：UNP residues 40-376
|
Not recorded | CU COPPER (II) ION × 6 CL CHLORIDE ION × 3 GOL GLYCEROL × 10 ACY ACETIC ACID × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.1；293 K；1:1 mixture of the purified protein solution (40 mg/ml) and a reservoir solution composed of 100 mM sodium acetate (pH 4.1) and 7% PEG 4000
|
Resolution 2.00 Å R-free 0.232 |
| 5F7A Nitrite complex structure of copper nitrite reductase from Alcaligenes faecalis determined at 293 K Deposited 2015-12-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Chain B
40–376（337 aa）
Chain C
40–376（337 aa）
|
Not recorded | CU COPPER (II) ION × 6 NO2 NITRITE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.1；293 K；100 mM sodium acetate, 7% PEG 4000
|
Resolution 1.54 Å R-free 0.180 |
| 5F7B Resting state structure of CuNiR form Alcaligenes faecalis determined at 293 K Deposited 2015-12-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
40–376（337 aa）
Chain B
40–376（337 aa）
Chain C
40–376（337 aa）
|
Not recorded | CU COPPER (II) ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.1；293 K；100 mM sodium acetate, 7% PEG 4000
|
Resolution 1.56 Å R-free 0.177 |