Current Protein Identity：P62333
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 5GJQ Structure of the human 26S proteasome bound to USP14-UbAl Deposited 2016-07-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count |
Chain L
1–389（389 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 2 seconds before plunging
|
Resolution 4.35 Å |
| 5GJR An atomic structure of the human 26S proteasome Deposited 2016-07-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 64 PDB declaration: 64-meric（64） Consistent with protein count |
Chain L
1–389（389 aa）
Chain z
1–389（389 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 12 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 2 seconds before plunging
|
Resolution 3.50 Å |
| 5L4G The human 26S proteasome at 3.9 A Deposited 2016-05-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain L
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å R-free 0.205 |
| 5LN3 The human 26S Proteasome at 6.8 Ang. Deposited 2016-08-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain L
1–389（389 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.80 Å |
| 5M32 Human 26S proteasome in complex with Oprozomib Deposited 2016-10-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 42-meric（42） Review required |
Chain f
1–379（379 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE；blot with sensor
|
Resolution 3.80 Å |
| 5T0C Structural basis for dynamic regulation of the human 26S proteasome Deposited 2016-08-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 64 PDB declaration: 64-meric（64） Consistent with protein count |
Chain AE
1–389（389 aa）
Chain BE
1–389（389 aa）
|
Not recorded | ZN ZINC ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 5VFP Nucleotide-driven Triple-state Remodeling of the AAA-ATPase Channel in the Activated Human 26S Proteasome Deposited 2017-04-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric（46） Consistent with protein count |
Chain E
15–389（375 aa）
|
Not recorded | ZN ZINC ION × 1 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 5VFQ Nucleotide-driven Triple-state Remodeling of the AAA-ATPase Channel in the Activated Human 26S Proteasome Deposited 2017-04-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric（46） Consistent with protein count |
Chain E
15–389（375 aa）
|
Not recorded | ZN ZINC ION × 1 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 5VFR Nucleotide-driven Triple-state Remodeling of the AAA-ATPase Channel in the Activated Human 26S Proteasome Deposited 2017-04-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric（46） Consistent with protein count |
Chain E
15–389（375 aa）
|
Not recorded | ZN ZINC ION × 1 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.90 Å |
| 5VFS Nucleotide-Driven Triple-State Remodeling of the AAA-ATPase Channel in the Activated Human 26S Proteasome Deposited 2017-04-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric（46） Consistent with protein count |
Chain E
15–389（375 aa）
|
Not recorded | ZN ZINC ION × 1 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 5VFT Nucleotide-driven Triple-state Remodeling of the AAA-ATPase Channel in the Activated Human 26S Proteasome Deposited 2017-04-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric（46） Consistent with protein count |
Chain E
37–389（353 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 5VFU Nucleotide-driven Triple-state Remodeling of the AAA-ATPase Channel in the Activated Human 26S Proteasome Deposited 2017-04-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric（46） Consistent with protein count |
Chain E
37–389（353 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.80 Å |
| 5VGZ Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle Deposited 2017-04-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric（17） Consistent with protein count |
Chain E
11–114（104 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å R-free 0.447 |
| 5VHF Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle Deposited 2017-04-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric（19） Consistent with protein count |
Chain E
11–389（379 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.70 Å |
| 5VHH Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle Deposited 2017-04-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric（19） Consistent with protein count |
Chain E
11–389（379 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.10 Å |
| 5VHI Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle Deposited 2017-04-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric（19） Consistent with protein count |
Chain E
11–389（379 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.80 Å |
| 5VHJ Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle Deposited 2017-04-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain E
128–389（262 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.50 Å |
| 5VHM Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle Deposited 2017-04-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain E
128–389（262 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.30 Å |
| 5VHN Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle Deposited 2017-04-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain E
115–389（275 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.30 Å |
| 5VHO Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle Deposited 2017-04-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain E
128–389（262 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.30 Å |
| 5VHP Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle Deposited 2017-04-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain E
128–389（262 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.90 Å |
| 5VHQ Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle Deposited 2017-04-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain E
128–389（262 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.90 Å |
| 5VHR Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle Deposited 2017-04-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain E
128–389（262 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.70 Å |
| 5VHS Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle Deposited 2017-04-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain E
11–389（379 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.80 Å |
| 6WJN SD-like state of human 26S Proteasome with non-cleavable M1-linked hexaubiquitin and E3 ubiquitin ligase E6AP/UBE3A Deposited 2020-04-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric（46） Consistent with protein count |
Chain E
15–389（375 aa）
|
Not recorded | ZN ZINC ION × 1 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；50 mM Tris, pH 7.5, 50 mM NaCl, 1.5 mM ATP-gamma-S, 5 mM MgCl2, 2 mM DTT, 10 uM zinc sulfate
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.70 Å |
| 8CVT Human 19S-20S proteasome, state SD2 Deposited 2022-05-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric（46） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 LDZ N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S)-4-methyl-1-oxopentan-2-yl]-L-leucinamide × 6 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8JRI Cryo-EM structure of human 26S proteasomal RP subcomplex (Ea state) without any bound substrate. Deposited 2023-06-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 25 PDB declaration: 25-meric（25） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；incubation time= 3 s
blotting time= 2.5 s
|
Resolution 3.40 Å |
| 8JRT Cryo-EM structure of human 26S proteasomal RP subcomplex (Ea state) bound to K11/K48-branched ubiquitin (Ub) chain composed of three Ub. Deposited 2023-06-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；incubation time= 3 s
blotting time= 2.5 s
|
Resolution 3.60 Å |
| 8JTI Cryo-EM structure of human 26S RP (Eb state) bound to K11/K48-branched ubiquitin (Ub) chain composed of four Ub. Deposited 2023-06-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric（29） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；incubation time= 3 s
blotting time= 2.5 s
|
Resolution 3.80 Å |
| 8K0G Cryo-EM structure of human 26S RP (Ed state) bound to K11/K48-branched ubiquitin (Ub) chain composed of four Ub. Deposited 2023-07-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；incubation time= 3 s
blotting time= 2.5 s
|
Resolution 3.80 Å |
| 8USB Non-substrate-engaged human 26S proteasome with Nub1/FAT10 bound to Rpn1 Deposited 2023-10-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric（27） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 5 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.73 Å |
| 8USC Nub1/Fat10-processing human 26S proteasome Deposited 2023-10-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | MG MAGNESIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 3 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9BUI M1A Midnolin-Proteasome (with Ubl) Deposited 2024-05-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 41 PDB declaration: 41-meric（41） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 9BV1 M2A Midnolin-Proteasome (translocating) Deposited 2024-05-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric（40） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9BV2 M2B Midnolin-Proteasome (translocating) Deposited 2024-05-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric（40） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9BV3 M1B Midnolin-Proteasome Deposited 2024-05-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric（40） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9BW4 TXNL1-bound proteasome Deposited 2024-05-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric（40） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9E8G Nub1/Fat10-processing human 26S proteasome bound to Txnl1 with Rpt5 at top of spiral staircase Deposited 2024-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric（35） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 3 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.01 Å |
| 9E8H Human proteasome in resting state conformation bound to TXNL1 in backward conformation Deposited 2024-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric（27） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9E8I Human proteasome in resting state conformation bound to TXNL1 in Forward conformation Deposited 2024-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric（27） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.87 Å |
| 9E8J Nub1/Fat10-processing human 26S proteasome bound to Txnl1 with Rpt1 at top of spiral staircase Deposited 2024-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.47 Å |
| 9E8K Nub1/Fat10-processing human 26S proteasome with Rpt6 at top of spiral staircase (AAA+ motor locally refined) Deposited 2024-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric（15） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.08 Å |
| 9E8L Nub1/Fat10-processing human 26S proteasome bound to Txnl1 with Rpt4 at top of spiral staircase Deposited 2024-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.59 Å |
| 9E8N Nub1/Fat10-processing human 26S proteasome bound to Txnl1 with Rpt3 at top of spiral staircase (locally refined on the AAA+ motor) Deposited 2024-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric（15） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.62 Å |
| 9E8O Nub1/Fat10-processing human 26S proteasome bound to Txnl1 with Rpt2 at top of spiral staircase and partially unfolded Eos Deposited 2024-11-05 | Assembly 1 Insufficient information Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9E8Q Nub1/Fat10-processing human 26S proteasome with Rpt2 at top of spiral staircase Deposited 2024-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric（33） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.16 Å |
| 9M2W The cryo-EM structure of 26S proteasome-Midnolin complex in the MD state Deposited 2025-02-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.31 Å |
| 9PDI Nub1/Fat10-processing human 26S proteasome with Rpt2 at top of spiral staircase and partially unfolded Eos (AAA+ motor locally refined) Deposited 2025-06-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric（15） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.98 Å |
| 9PDL Nub1/Fat10-processing human 26S proteasome with Rpt5 at top of spiral staircase (AAA+ locally refined) Deposited 2025-06-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric（15） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 5 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.76 Å |
| 9PDN Nub1/Fat10-processing human 26S proteasome with Rpt1 at top of spiral staircase (AAA+ locally refined) Deposited 2025-06-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric（15） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |
| 9PF1 Nub1/Fat10-processing human 26S proteasome with Rpt4 at top of spiral staircase (AAA+ motor locally refined) Deposited 2025-07-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric（15） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.57 Å |
| 9UF8 The cryo-EM structure of 26S proteasome-Midnolin complex in the MA state Deposited 2025-04-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.32 Å |
| 9UG9 The cryo-EM structure of 26S proteasome-Midnolin complex MB state Deposited 2025-04-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9W39 Structure of human 26S proteasome complexed with midnolin, 19S proteasome with Ubl bound Deposited 2025-07-29 | Assembly 1 Insufficient information Heteromer；Protein × 19 PDB declaration: 19-meric（19） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 1 MG MAGNESIUM ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.65 Å |
| 9WBG Structure of human 26S proteasome complexed with midnolin, 19S proteasome with Ubl and Catch domain resolved Deposited 2025-08-14 | Assembly 1 Insufficient information Heteromer；Protein × 20 PDB declaration: 20-meric（20） Consistent with protein count |
Chain E
1–389（389 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 1 MG MAGNESIUM ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.23 Å |