Current Protein Identity：P68363
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2E4H Solution structure of cytoskeletal protein in complex with tubulin tail Deposited 2006-12-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
416–451（36 aa）
Fragment：C-tail, residues 416-451
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.9；303 K；Pressure ambient
NMR sample composition
0.9mM U-15N,13C CLIP: Nonlabel tubulin; 30mM BisTris buffer K; 1mM DTT; 100% D2O | 100% D2O
NMR sample composition
0.9mM U-15N CLIP: Nonlabel tubulin; 30mM BisTris buffer K; 1mM DTT; 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition
0.9mM U-15N,13C tubulin: Nonlabel CLIP; 30mM BisTris buffer K; 1mM DTT; 100% D2O | 100% D2O
NMR sample composition
0.9mM U-15N tubulin: Nonlabel CLIP; 30mM BisTris buffer K; 1mM DTT; 90% H2O, 10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 5IJ0 Cryo EM density of microtubule assembled from human TUBB3 Deposited 2016-03-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–437（437 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 5IJ9 Cryo EM density of microtubule assembled from human TUBB3-D417H mutant Deposited 2016-03-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–437（437 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 5N5N Cryo-EM structure of tsA201 cell alpha1B and betaI and betaIVb microtubules Deposited 2017-02-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain G
1–437（437 aa）
Chain H
1–437（437 aa）
Chain I
1–437（437 aa）
Chain J
1–437（437 aa）
Chain K
1–437（437 aa）
Chain L
1–437（437 aa）
|
Not recorded | G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 MG MAGNESIUM ION × 12 GTP GUANOSINE-5'-TRIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB80 with GMPCPP (80mM PIPES, 2mM MgCl2, 1mM EGTA, 1mM DTT, 1mM GMPCPP)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6E7B 13-pf 3-start GMPCPP-human alpha1B/beta3 microtubules Deposited 2018-07-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–437（437 aa）
|
Not recorded | MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6E7C 14-pf 3-start GMPCPP-human alpha1B/beta2B microtubules Deposited 2018-07-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–437（437 aa）
|
Not recorded | MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.65 Å |
| 6I2I Refined 13pf Hela Cell Tubulin microtubule (EML4-NTD decorated) Deposited 2018-11-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；25mM PIPES, 1.5mM MgCl2, 1mM EGTA, 1mM DTT, 30mM NaCl,1mM GMPCPP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6J4V Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications Deposited 2019-01-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
431–451（21 aa）
|
Mutation：E450C | PO4 PHOSPHATE ION × 2 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；pH 8.2；293 K；For crystallization of V2c-SVBP C58S in complex with the maTail peptide the components were mixed in a 1:3 molar ratio and incubated at room temperature for 24 h before setting up crystallization trials. The resulting V2c-SVBP S58C-maTail complex was crystallized in 1.4 M sodium/potassium phosphate, pH 8.2.
|
Resolution 2.10 Å R-free 0.200 |
| 6QUS HsCKK (human CAMSAP1) decorated 13pf taxol-GDP microtubule Deposited 2019-02-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain O
1–451（451 aa）
Chain X
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6QUY NgCKK (N.Gruberi CKK) decorated 13pf taxol-GDP microtubule Deposited 2019-02-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6QVE NgCKK (Naegleria Gruberi CKK) decorated 14pf taxol-GDP microtubule Deposited 2019-03-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6QVJ HsCKK (human CAMSAP1) decorated 14pf taxol-GDP microtubule Deposited 2019-03-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain O
1–451（451 aa）
Chain X
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6S8L Structure, Thermodynamics, and Kinetics of Plinabulin Binding to two Tubulin Isotypes Deposited 2019-07-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 PN6 (3Z,6Z)-3-benzylidene-6-[(5-tert-butyl-1H-imidazol-4-yl)methylidene]piperazine-2,5-dione × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；100 mM Bis-TrisMethane, pH 5.5, supplemented with 200 mM ammonium sulfate and 25% PEG3350
|
Resolution 1.80 Å R-free 0.266 |
| 7LXB HeLa-tubulin in complex with cryptophycin 52 Deposited 2021-03-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric（16） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
Chain E
1–451（451 aa）
Chain G
1–451（451 aa）
Chain I
1–451（451 aa）
Chain K
1–451（451 aa）
Chain M
1–451（451 aa）
Chain O
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 8 YGY Cryptophycin 52 × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.26 Å |
| 7M18 HeLa-tubulin in complex with cryptophycin 1 Deposited 2021-03-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric（16） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
Chain E
1–451（451 aa）
Chain G
1–451（451 aa）
Chain I
1–451（451 aa）
Chain K
1–451（451 aa）
Chain M
1–451（451 aa）
Chain O
1–451（451 aa）
|
Not recorded | YNP Cryptophycin 1 × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.38 Å |
| 7M20 18-mer HeLa-tubulin rings in complex with Cryptophycin 1 Deposited 2021-03-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
Chain E
1–451（451 aa）
Chain G
1–451（451 aa）
Chain I
1–451（451 aa）
Chain K
1–451（451 aa）
Chain M
1–451（451 aa）
Chain O
1–451（451 aa）
Chain Q
1–451（451 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 9 YNP Cryptophycin 1 × 9 GTP GUANOSINE-5'-TRIPHOSPHATE × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.84 Å |
| 7PJF Inhibiting parasite proliferation using a rationally designed anti-tubulin agent Deposited 2021-08-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.5；293 K；100 mM Bis-TrisMethane, pH 5.5, supplemented with 200 mM ammonium sulfate and 25% PEG3350
|
Resolution 1.86 Å R-free 0.218 |
| 7SJ7 Undecorated 13pf wildtype microtubule from recombinant human tubulin Deposited 2021-10-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
Chain E
1–451（451 aa）
Chain J
1–451（451 aa）
Chain K
1–451（451 aa）
Chain L
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；80 mM PIPES, 1 mM EGTA, 5 mM MgCl2, 2 mM GTP, pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.80 Å |
| 7SJ8 13pf wildtype microtubule from recombinant human tubulin decorated with kinesin Deposited 2021-10-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
Chain E
1–451（451 aa）
Chain J
1–451（451 aa）
Chain K
1–451（451 aa）
Chain L
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；80 mM PIPES, 1 mM EGTA, 5 mM MgCl2, 2 mM GTP, pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；30 second adsorption time onto grid. blot force 15, 4 second blot time, 37 degrees, and 100 percent humidity. 0.5 second drain time.
|
Resolution 3.60 Å |
| 7SJ9 13pf E254A microtubule from recombinant human tubulin decorated with EB3 Deposited 2021-10-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
Chain E
1–451（451 aa）
Chain J
1–451（451 aa）
Chain K
1–451（451 aa）
Chain L
1–451（451 aa）
|
Mutation：E254A Mutation：E254A Mutation：E254A Mutation：E254A Mutation：E254A Mutation：E254A | GTP GUANOSINE-5'-TRIPHOSPHATE × 12 MG MAGNESIUM ION × 12 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；80 mM PIPES, 1 mM EGTA, 5 mM MgCl2, 2 mM GTP, pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.80 Å |
| 7SJA Undecorated 13pf E254N microtubule from recombinant human tubulin Deposited 2021-10-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
Chain E
1–451（451 aa）
Chain J
1–451（451 aa）
Chain K
1–451（451 aa）
Chain L
1–451（451 aa）
|
Mutation：E254N Mutation：E254N Mutation：E254N Mutation：E254N Mutation：E254N Mutation：E254N | GTP GUANOSINE-5'-TRIPHOSPHATE × 12 MG MAGNESIUM ION × 12 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；80 mM PIPES, 1 mM EGTA, 5 mM MgCl2, 2 mM GTP, pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.80 Å |
| 7Z6S MATCAP bound to a human 14 protofilament microtubule Deposited 2022-03-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: Hexameric（6） Consistent with protein count |
Chain A
1–451（451 aa）
Chain K
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7ZCW Cryo-EM structure of GMPCPP-microtubules in complex with VASH2-SVBP Deposited 2022-03-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–451（451 aa）
Chain E
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 6 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8T42 Model of TTLL6 MTBH1-2 bound to microtubule Deposited 2023-06-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain A
1–451（451 aa）
Chain F
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；The sample was blotted with Whatman #5 blotting paper on both sides of the grid for 3 s with a blot offset of -1 using a Vitrobot Mark IV (Thermo Fisher Scientific) with a chamber set to 30C at 100% humidity and subsequently plunged into liquid ethane.
|
Resolution 3.60 Å |
| 8U3Z Model of TTLL6 bound to microtubule from composite map Deposited 2023-09-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain A
1–451（451 aa）
Chain F
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8V2J Structure of alpha1B and betaI/IVb microtubule bound to GDP Deposited 2023-11-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–451（451 aa）
Chain D
1–451（451 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；5 ul of microtubules were applied to glow-discharged cryo-EM grid and allowed to absorb for 30 sec.
|
Resolution 2.90 Å |
| 8VA2 Symmetry expanded map of 2 gamma-tubulins bound to 2 alpha tubulins in gamma tubulin ring complex capped microtubule end. Deposited 2023-12-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain a
1–451（451 aa）
Chain b
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 8VRJ Rigid body fitted model for gamma tubulin ring complex capped microtubule Deposited 2024-01-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 60 PDB declaration: 60-meric（60） Consistent with protein count |
Chain 1
1–451（451 aa）
Chain O
1–451（451 aa）
Chain P
1–451（451 aa）
Chain Q
1–451（451 aa）
Chain R
1–451（451 aa）
Chain S
1–451（451 aa）
Chain T
1–451（451 aa）
Chain U
1–451（451 aa）
Chain V
1–451（451 aa）
Chain W
1–451（451 aa）
Chain X
1–451（451 aa）
Chain Y
1–451（451 aa）
Chain Z
1–451（451 aa）
|
Mutation：E254D Mutation：E254D Mutation：E254D Mutation：E254D Mutation：E254D Mutation：E254D Mutation：E254D Mutation：E254D Mutation：E254D Mutation：E254D Mutation：E254D Mutation：E254D Mutation：E254D | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.70 Å |
| 8VRK Rigid body fitted model for refined density map of gamma tubulin ring complex capped microtubule Deposited 2024-01-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 60 PDB declaration: 60-meric（60） Consistent with protein count |
Chain 1
1–451（451 aa）
Chain O
1–451（451 aa）
Chain P
1–451（451 aa）
Chain Q
1–451（451 aa）
Chain R
1–451（451 aa）
Chain S
1–451（451 aa）
Chain T
1–451（451 aa）
Chain U
1–451（451 aa）
Chain V
1–451（451 aa）
Chain W
1–451（451 aa）
Chain X
1–451（451 aa）
Chain Y
1–451（451 aa）
Chain Z
1–451（451 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.50 Å |
| 8VT7 Structure of the gamma tubulin ring complex nucleated microtubule protofilament. Deposited 2024-01-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–451（451 aa）
Chain J
1–451（451 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.66 Å |
| 9BP6 Structure of alpha1B and betaI/IVb microtubule bound to GMPCPP Deposited 2024-05-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–451（451 aa）
Chain I
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；Double-cycled GMPCPP microtubules were diluted to 2.5uM in Brb80 buffer. 5 ul of microtubules were applied to glow-discharged cryo-EM grid and allowed to absorb for 30 sec.
|
Resolution 3.10 Å |
| 9CMM Two protofilament structure of alpha1B and betaI/IVb microtubule bound to GMPCPP Deposited 2024-07-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
Chain E
1–451（451 aa）
Chain I
1–451（451 aa）
|
Not recorded | G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 4 MG MAGNESIUM ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；Double-cycled GMPCPP microtubules were diluted to 2.5uM in Brb80 buffer. 5 ul of microtubules were applied to glow-discharged cryo-EM grid and allowed to absorb for 30 sec.
|
Resolution 3.10 Å |
| 9COC Two protofilament structure of alpha1B and betaI/IVb microtubule bound to GDP Deposited 2024-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
Chain D
1–451（451 aa）
Chain I
1–451（451 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；5 ul of microtubules were applied to glow-discharged electron microscopy grids and allowed to absorb for 30 sec.
|
Resolution 2.90 Å |
| 9F3B Undecorated 13pf E254Q microtubule from recombinant human tubulin Deposited 2024-04-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric（12） Consistent with protein count |
Chain A
1–42（42 aa）
Chain A
47–441（395 aa）
Chain C
1–42（42 aa）
Chain C
47–441（395 aa）
Chain E
1–42（42 aa）
Chain E
47–441（395 aa）
Chain G
1–42（42 aa）
Chain G
47–441（395 aa）
Chain I
1–42（42 aa）
Chain I
47–441（395 aa）
Chain K
1–42（42 aa）
Chain K
47–441（395 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 12 MG MAGNESIUM ION × 12 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9F3H Undecorated 13pf mosaic 20%E254Q - 80% E254QN microtubule from recombinant human tubulin Deposited 2024-04-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric（12） Consistent with protein count |
Chain A
47–441（395 aa）
Chain C
47–441（395 aa）
Chain E
47–441（395 aa）
Chain G
47–441（395 aa）
Chain I
47–441（395 aa）
Chain K
47–441（395 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 12 MG MAGNESIUM ION × 12 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 9F3R 13pf E254Q microtubule from recombinant human tubulin decorated with EB3 Deposited 2024-04-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: 14-meric（14） Consistent with protein count |
Chain A
1–42（42 aa）
Chain A
47–441（395 aa）
Chain C
1–42（42 aa）
Chain C
47–441（395 aa）
Chain E
1–42（42 aa）
Chain E
47–441（395 aa）
Chain G
1–42（42 aa）
Chain G
47–441（395 aa）
Chain I
1–42（42 aa）
Chain I
47–441（395 aa）
Chain K
1–42（42 aa）
Chain K
47–441（395 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 12 MG MAGNESIUM ION × 12 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 9F3S 13pf mosaic 20%E254Q - 80% E254N microtubule from recombinant human tubulin decorated with EB3 Deposited 2024-04-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: 14-meric（14） Consistent with protein count |
Chain A
1–42（42 aa）
Chain A
47–441（395 aa）
Chain C
1–42（42 aa）
Chain C
47–441（395 aa）
Chain E
1–42（42 aa）
Chain E
47–441（395 aa）
Chain G
1–42（42 aa）
Chain G
47–441（395 aa）
Chain I
1–42（42 aa）
Chain I
47–441（395 aa）
Chain K
1–42（42 aa）
Chain K
47–441（395 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 12 MG MAGNESIUM ION × 12 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 9HQ4 TTLL11 bound to microtubule Deposited 2024-12-16 | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
|
Not recorded | G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 4 MG MAGNESIUM ION × 4 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7；40 mM Tris-HCl, pH 7.0, 1 mM TCEP, 1 mM MgCl2, 5 % glycerol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.28 Å |
| 9SMX CM1-activated gTuRC in complex with nascent alpha-E254D mutant microtubules Deposited 2025-09-09 | Assembly 1 Insufficient information Heteromer；Protein × 73 PDB declaration: 73-meric（73） Consistent with protein count |
Chain BA
1–451（451 aa）
Chain CA
1–451（451 aa）
Chain DA
1–451（451 aa）
Chain EA
1–451（451 aa）
Chain FA
1–451（451 aa）
Chain GA
1–451（451 aa）
Chain HA
1–451（451 aa）
Chain IA
1–451（451 aa）
Chain JA
1–451（451 aa）
Chain KA
1–451（451 aa）
Chain LA
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.67 Å |