Current Protein Identity：Q15465
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3HO5 Crystal structure of Hedgehog-interacting protein (HHIP) and Sonic hedgehog (SHH) complex Deposited 2009-06-01 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain H
29–197（169 aa）
Fragment：UNP residues 29-197
|
Not recorded | ZN ZINC ION × 1 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；292 K；0.1 M bis-Tris propane pH 7.0 and 2.2-2.5 M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 3.01 Å R-free 0.287 |
| 3M1N Crystal structure of Human Sonic Hedgehog N-terminal domain Deposited 2010-03-05 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
23–197（175 aa）
Fragment：Sonic hedgehog protein N-product
|
Mutation：A23I, C24I | ZN ZINC ION × 1 SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；273 K；21-24% PEG 4000, 200 mM LiSO4, and 100 mM Tris, pH = 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K
|
Resolution 1.85 Å R-free 0.260 |
| 3M1N Crystal structure of Human Sonic Hedgehog N-terminal domain Deposited 2010-03-05 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
23–197（175 aa）
Fragment：Sonic hedgehog protein N-product
|
Mutation：A23I, C24I | ZN ZINC ION × 1 SO4 SULFATE ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；273 K；21-24% PEG 4000, 200 mM LiSO4, and 100 mM Tris, pH = 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K
|
Resolution 1.85 Å R-free 0.260 |
| 3MXW Crystal structure Sonic hedgehog bound to the 5E1 fab fragment Deposited 2010-05-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
29–197（169 aa）
|
Not recorded | ZN ZINC ION × 1 CA CALCIUM ION × 2 SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；protein solution (20 mM Hepes, pH 7.2, 0.1 M NaCl, 5 mM CaCl2) was mixed with equal volume of well solution (0.2 M lithium sulfate, 100 mM Hepes, pH 7.5, and 22% w/v PEG 4000), VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 1.83 Å R-free 0.218 |
| 6DMY Cryo-EM structure of human Ptch1 and ShhN complex Deposited 2018-06-05 | Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
24–197（174 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 Y01 CHOLESTEROL HEMISUCCINATE × 2 SER SERINE × 1 ZN ZINC ION × 1 CA CALCIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6E1H Structure of 2:1 human Ptch1-Shh-N complex Deposited 2018-07-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
24–197（174 aa）
|
Not recorded | ZN ZINC ION × 1 CA CALCIUM ION × 2 PLM PALMITIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
|
Resolution 3.50 Å |
| 6N7G Cryo-EM structure of tetrameric Ptch1 in complex with ShhNp (form I) Deposited 2018-11-27 | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain C
24–197（174 aa）
Chain F
24–197（174 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 20 CLR CHOLESTEROL × 10 ZN ZINC ION × 2 CA CALCIUM ION × 4 PLM PALMITIC ACID × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.80 Å |
| 6N7H Cryo-EM structure of the 2:1 hPtch1-Shhp complex Deposited 2018-11-27 | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
24–197（174 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 10 CLR CHOLESTEROL × 5 ZN ZINC ION × 1 CA CALCIUM ION × 2 PLM PALMITIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6N7K Cryo-EM structure of tetrameric Ptch1 in complex with ShhNp (form II) Deposited 2018-11-27 | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain C
24–197（174 aa）
Chain F
24–197（174 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 20 CLR CHOLESTEROL × 10 ZN ZINC ION × 2 CA CALCIUM ION × 4 PLM PALMITIC ACID × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.50 Å |
| 6OEV Structure of human Patched1 in complex with native Sonic Hedgehog Deposited 2019-03-27 | Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
23–197（175 aa）
Fragment：residues 24-197
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
|
Resolution 3.80 Å |
| 6PJV Structure of Human Sonic Hedgehog in complex with Zinc and Magnesium Deposited 2019-06-28 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
29–197（169 aa）
|
Not recorded | DMS DIMETHYL SULFOXIDE × 3 GOL GLYCEROL × 1 MG MAGNESIUM ION × 2 ZN ZINC ION × 1 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；24% (w/v) polyethylene glycol 8000, 0.10 M TRIS pH 7.0, and 0.24 M MgCl2
|
Resolution 1.43 Å R-free 0.139 |
| 6RMG Structure of PTCH1 bound to a modified Hedgehog ligand ShhN-C24II Deposited 2019-05-06 | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
21–197（177 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 Y01 CHOLESTEROL HEMISUCCINATE × 11 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 6RVD Revised cryo-EM structure of the human 2:1 Ptch1-Shh complex Deposited 2019-05-31 | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
24–197（174 aa）
|
Not recorded | CLR CHOLESTEROL × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 7 PLM PALMITIC ACID × 1 ZN ZINC ION × 1 CA CALCIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20 mM Hepes pH 7.5,
150 mM NaCl,
0.06% Digitonin,
1mM CaCl2.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7E2I Cryo-EM structure of hDisp1NNN-ShhN Deposited 2021-02-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
1–462（462 aa）
|
Not recorded | ZN ZINC ION × 1 Y01 CHOLESTEROL HEMISUCCINATE × 7 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.07 Å |
| 7MHZ Human Hedgehog acyltransferase (HHAT) in complex with a palmitoylated Hedgehog peptide product and a Fab antibody fragment Deposited 2021-04-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
24–31（8 aa）
Fragment：UNP residues 24-31
|
Not recorded | PLM PALMITIC ACID × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 PKZ Palmitoyl-CoA × 3 AJP Digitonin × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 7RHQ Cryo-EM structure of Xenopus Patched-1 in complex with GAS1 and Sonic Hedgehog Deposited 2021-07-18 | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
24–197（174 aa）
|
Not recorded | CLR CHOLESTEROL × 2 Y01 CHOLESTEROL HEMISUCCINATE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ZN ZINC ION × 1 CA CALCIUM ION × 2 PLM PALMITIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.53 Å |
| 8YYZ Crystal structure of Sonic hedgehog in complex with antibody 5E1 mutant H-R102A in the absence of metals Deposited 2024-04-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
29–197（169 aa）
|
Not recorded | CL CHLORIDE ION × 5 GOL GLYCEROL × 1 IOD IODIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；0.2M Ammonium iodide, 20% w/v Polyethylene glycol 3350
|
Resolution 1.90 Å R-free 0.222 |
| 8Z2V Crystal structure of Sonic hedgehog in complex with antibody 5E1 mutant H-R102A with metals Deposited 2024-04-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
29–197（169 aa）
|
Not recorded | CL CHLORIDE ION × 1 ZN ZINC ION × 1 CA CALCIUM ION × 2 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；200mM MgCl2, 20% PEG 3350
|
Resolution 1.89 Å R-free 0.225 |
| 8Z39 Crystal structure of Sonic hedgehog in complex with antibody 5E1 mutant L-T56A with metals Deposited 2024-04-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
29–197（169 aa）
|
Not recorded | CA CALCIUM ION × 2 ZN ZINC ION × 1 CL CHLORIDE ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；200mM NaCl, 20% PEG 3350
|
Resolution 1.80 Å R-free 0.208 |
| 8Z3A Crystal structure of Sonic hedgehog in complex with antibody 5E1 without metals Deposited 2024-04-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
29–197（169 aa）
|
Not recorded | GOL GLYCEROL × 1 NO3 NITRATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；200mM KNO3, 20% PEG 3350
|
Resolution 1.75 Å R-free 0.207 |