当前蛋白身份：Q38087
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1B8H SLIDING CLAMP, DNA POLYMERASE 提交 1999-02-01 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 D
893–903（11 aa）
片段：RESIDUES 893-903
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；pH 7.5, VAPOR DIFFUSION, HANGING DROP
|
分辨率 3.00 Å R-free 0.313 |
| 1CLQ CRYSTAL STRUCTURE OF A REPLICATION FORK DNA POLYMERASE EDITING COMPLEX AT 2.7 A RESOLUTION 提交 1999-04-30 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
片段：RESIDUES 1-903
|
未记录 | CA CALCIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；285 K；12% (W/V) PEG 350MME, 150 MM CACL2, 100 MM SODIUM CACODYLATE, PH 6.5, 12 DEGREES C, VAPOR DIFFUSION, HANGING DROP, temperature 285K
|
分辨率 2.70 Å R-free 0.284 |
| 1IG9 Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase 提交 2001-04-17 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：Exonuclease-deficient (D222A/D327A) mutant | CA CALCIUM ION × 4 TTP THYMIDINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.25；289 K；PEG 350 monomethyl ether, calcium chloride, sodium cacodylate, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 2.60 Å R-free 0.258 |
| 1IH7 High-Resolution Structure of Apo RB69 DNA Polymerase 提交 2001-04-18 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–903（903 aa）
|
未记录 | K POTASSIUM ION × 2 GMP GUANOSINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 5.6；285 K；Sodium citrate, sodium/potassium tartrate, ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 285K
|
分辨率 2.21 Å R-free 0.258 |
| 1Q9X Crystal structure of Enterobacteria phage RB69 gp43 DNA polymerase complexed with tetrahydrofuran containing DNA 提交 2003-08-26 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A | 3DR 1',2'-DIDEOXYRIBOFURANOSE-5'-PHOSPHATE × 1 DOC 2',3'-DIDEOXYCYTIDINE-5'-MONOPHOSPHATE × 1 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；298 K；PEG 350MME, Calcium chloride, Tris-HCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.50
|
分辨率 2.69 Å R-free 0.288 |
| 1Q9X Crystal structure of Enterobacteria phage RB69 gp43 DNA polymerase complexed with tetrahydrofuran containing DNA 提交 2003-08-26 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A, D327A | 3DR 1',2'-DIDEOXYRIBOFURANOSE-5'-PHOSPHATE × 1 DOC 2',3'-DIDEOXYCYTIDINE-5'-MONOPHOSPHATE × 1 DGP 2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE × 1 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；298 K；PEG 350MME, Calcium chloride, Tris-HCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.50
|
分辨率 2.69 Å R-free 0.288 |
| 1Q9X Crystal structure of Enterobacteria phage RB69 gp43 DNA polymerase complexed with tetrahydrofuran containing DNA 提交 2003-08-26 | Assembly 3 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–903（903 aa）
|
突变：D222A, D327A | 3DR 1',2'-DIDEOXYRIBOFURANOSE-5'-PHOSPHATE × 1 DOC 2',3'-DIDEOXYCYTIDINE-5'-MONOPHOSPHATE × 1 DGP 2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE × 3 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；298 K；PEG 350MME, Calcium chloride, Tris-HCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.50
|
分辨率 2.69 Å R-free 0.288 |
| 1Q9X Crystal structure of Enterobacteria phage RB69 gp43 DNA polymerase complexed with tetrahydrofuran containing DNA 提交 2003-08-26 | Assembly 4 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 D
1–903（903 aa）
|
突变：D222A, D327A | 3DR 1',2'-DIDEOXYRIBOFURANOSE-5'-PHOSPHATE × 1 DOC 2',3'-DIDEOXYCYTIDINE-5'-MONOPHOSPHATE × 1 DGP 2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；298 K；PEG 350MME, Calcium chloride, Tris-HCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.50
|
分辨率 2.69 Å R-free 0.288 |
| 1Q9Y CRYSTAL STRUCTURE OF ENTEROBACTERIA PHAGE RB69 GP43 DNA POLYMERASE COMPLEXED WITH 8-OXOGUANOSINE CONTAINING DNA 提交 2003-08-26 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；PEG 350MME, Calcium chloride, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.50
|
分辨率 2.80 Å R-free 0.271 |
| 1WAF DNA POLYMERASE FROM BACTERIOPHAGE RB69 提交 1997-04-13 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–903（903 aa）
|
未记录 | GMP GUANOSINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；pH 5.6
|
分辨率 3.20 Å R-free 0.274 |
| 1WAF DNA POLYMERASE FROM BACTERIOPHAGE RB69 提交 1997-04-13 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–903（903 aa）
|
未记录 | GMP GUANOSINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；pH 5.6
|
分辨率 3.20 Å R-free 0.274 |
| 1WAJ DNA POLYMERASE FROM BACTERIOPHAGE RB69 提交 1997-04-13 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–903（903 aa）
|
未记录 | 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；pH 5.6
|
分辨率 2.80 Å R-free 0.280 |
| 2ATQ RB69 single-stranded DNA binding protein-DNA polymerase fusion 提交 2005-08-25 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A | GDP GUANOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；PEG 400, Tris-Cl, 6-aminocaproic acid, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
分辨率 3.20 Å R-free 0.345 |
| 2DTU Crystal structure of the beta hairpin loop deletion variant of RB69 gp43 in complex with DNA containing an abasic site analog 提交 2006-07-15 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–902（902 aa）
|
突变：D222A, D327A, I253G, Deletion 254-260 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.1；293 K；PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES pH 7.1, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.37 Å R-free 0.268 |
| 2DTU Crystal structure of the beta hairpin loop deletion variant of RB69 gp43 in complex with DNA containing an abasic site analog 提交 2006-07-15 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–902（902 aa）
|
突变：D222A, D327A, I253G, Deletion 254-260 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.1；293 K；PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES pH 7.1, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.37 Å R-free 0.268 |
| 2DTU Crystal structure of the beta hairpin loop deletion variant of RB69 gp43 in complex with DNA containing an abasic site analog 提交 2006-07-15 | Assembly 3 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–902（902 aa）
|
突变：D222A, D327A, I253G, Deletion 254-260 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.1；293 K；PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES pH 7.1, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.37 Å R-free 0.268 |
| 2DTU Crystal structure of the beta hairpin loop deletion variant of RB69 gp43 in complex with DNA containing an abasic site analog 提交 2006-07-15 | Assembly 4 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 D
1–902（902 aa）
|
突变：D222A, D327A, I253G, Deletion 254-260 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.1；293 K；PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES pH 7.1, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.37 Å R-free 0.268 |
| 2DY4 Crystal structure of RB69 GP43 in complex with DNA containing Thymine Glycol 提交 2006-09-06 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG2000 MME, Magnesium Sulfate, Sodium acetate, HEPES buffer pH7.0, Beta-mercaptoethanol, glycerol, 3 cycles of Macro-seeding, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.65 Å R-free 0.281 |
| 2DY4 Crystal structure of RB69 GP43 in complex with DNA containing Thymine Glycol 提交 2006-09-06 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A, D327A 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG2000 MME, Magnesium Sulfate, Sodium acetate, HEPES buffer pH7.0, Beta-mercaptoethanol, glycerol, 3 cycles of Macro-seeding, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.65 Å R-free 0.281 |
| 2DY4 Crystal structure of RB69 GP43 in complex with DNA containing Thymine Glycol 提交 2006-09-06 | Assembly 3 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–903（903 aa）
|
突变：D222A, D327A 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG2000 MME, Magnesium Sulfate, Sodium acetate, HEPES buffer pH7.0, Beta-mercaptoethanol, glycerol, 3 cycles of Macro-seeding, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.65 Å R-free 0.281 |
| 2DY4 Crystal structure of RB69 GP43 in complex with DNA containing Thymine Glycol 提交 2006-09-06 | Assembly 4 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 D
1–903（903 aa）
|
突变：D222A, D327A 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG2000 MME, Magnesium Sulfate, Sodium acetate, HEPES buffer pH7.0, Beta-mercaptoethanol, glycerol, 3 cycles of Macro-seeding, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.65 Å R-free 0.281 |
| 2OYQ Crystal structure of RB69 gp43 in complex with DNA with 5-NIMP opposite an abasic site analog 提交 2007-02-22 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A | N5P 1-{2-DEOXY-5-O-[(R)-HYDROXY{[(R)-HYDROXY(PHOSPHONOOXY)PHOSPHORYL]OXY}PHOSPHORYL]-BETA-D-ERYTHRO-PENTOFURANOSYL}-5-NITRO -1H-INDOLE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.5, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.86 Å R-free 0.294 |
| 2OYQ Crystal structure of RB69 gp43 in complex with DNA with 5-NIMP opposite an abasic site analog 提交 2007-02-22 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.5, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.86 Å R-free 0.294 |
| 2OYQ Crystal structure of RB69 gp43 in complex with DNA with 5-NIMP opposite an abasic site analog 提交 2007-02-22 | Assembly 3 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–903（903 aa）
|
突变：D222A, D327A | MG MAGNESIUM ION × 1 N5P 1-{2-DEOXY-5-O-[(R)-HYDROXY{[(R)-HYDROXY(PHOSPHONOOXY)PHOSPHORYL]OXY}PHOSPHORYL]-BETA-D-ERYTHRO-PENTOFURANOSYL}-5-NITRO -1H-INDOLE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.5, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.86 Å R-free 0.294 |
| 2OYQ Crystal structure of RB69 gp43 in complex with DNA with 5-NIMP opposite an abasic site analog 提交 2007-02-22 | Assembly 4 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 D
1–903（903 aa）
|
突变：D222A, D327A | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.5, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.86 Å R-free 0.294 |
| 2OZM Crystal structure of RB69 gp43 in complex with DNA with 5-NITP opposite an abasic site analog 提交 2007-02-26 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A | MG MAGNESIUM ION × 1 N5P 1-{2-DEOXY-5-O-[(R)-HYDROXY{[(R)-HYDROXY(PHOSPHONOOXY)PHOSPHORYL]OXY}PHOSPHORYL]-BETA-D-ERYTHRO-PENTOFURANOSYL}-5-NITRO -1H-INDOLE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；PEG 20000,sodium acetate, magnesium acetate, beta-mercaptoethanol, TRIS 7.5, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.86 Å R-free 0.268 |
| 2OZS Crystal structure of RB69 gp43 in complex with DNA with dATP opposite dTMP 提交 2007-02-27 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A | MG MAGNESIUM ION × 1 DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 20000, sodium acetate, magnesium acetate, beta-mercaptoethanol, TRIS 7.0, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.75 Å R-free 0.272 |
| 2P5G Crystal structure of RB69 gp43 in complex with DNA with dAMP opposite an abasic site analog in a 21mer template 提交 2007-03-15 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.0, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.80 Å R-free 0.295 |
| 2P5G Crystal structure of RB69 gp43 in complex with DNA with dAMP opposite an abasic site analog in a 21mer template 提交 2007-03-15 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A,D327A 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.0, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.80 Å R-free 0.295 |
| 2P5G Crystal structure of RB69 gp43 in complex with DNA with dAMP opposite an abasic site analog in a 21mer template 提交 2007-03-15 | Assembly 3 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–903（903 aa）
|
突变：D222A,D327A 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.0, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.80 Å R-free 0.295 |
| 2P5G Crystal structure of RB69 gp43 in complex with DNA with dAMP opposite an abasic site analog in a 21mer template 提交 2007-03-15 | Assembly 4 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 D
1–903（903 aa）
|
突变：D222A,D327A 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.0, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.80 Å R-free 0.295 |
| 2P5O Crystal structure of RB69 GP43 in complex with DNA containing an abasic site analog 提交 2007-03-15 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
2–903（902 aa）
|
突变：D222A, D327A 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；8% PEG 2000 MME. 100mM Magnesium sulfate, 100mM HEPES, 100mM Sodium acetate, 15% glycerol, 2mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.80 Å R-free 0.290 |
| 2P5O Crystal structure of RB69 GP43 in complex with DNA containing an abasic site analog 提交 2007-03-15 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
2–903（902 aa）
|
突变：D222A, D327A 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；8% PEG 2000 MME. 100mM Magnesium sulfate, 100mM HEPES, 100mM Sodium acetate, 15% glycerol, 2mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.80 Å R-free 0.290 |
| 2P5O Crystal structure of RB69 GP43 in complex with DNA containing an abasic site analog 提交 2007-03-15 | Assembly 3 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
2–903（902 aa）
|
突变：D222A, D327A 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；8% PEG 2000 MME. 100mM Magnesium sulfate, 100mM HEPES, 100mM Sodium acetate, 15% glycerol, 2mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.80 Å R-free 0.290 |
| 2P5O Crystal structure of RB69 GP43 in complex with DNA containing an abasic site analog 提交 2007-03-15 | Assembly 4 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 D
2–903（902 aa）
|
突变：D222A, D327A 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；8% PEG 2000 MME. 100mM Magnesium sulfate, 100mM HEPES, 100mM Sodium acetate, 15% glycerol, 2mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.80 Å R-free 0.290 |
| 3CFO Triple Mutant APO structure 提交 2008-03-04 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–903（903 aa）
|
突变：L561A/S565G/Y567A | SO4 SULFATE ION × 4 GMP GUANOSINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；0.015 M MG ACETATE, 0.05M NA CACODYLATE, 1.7 M (NH4)2SO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.60 Å R-free 0.235 |
| 3CFP Structure of the replicating complex of a POL Alpha family DNA Polymerase, ternary complex 1 提交 2008-03-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：L561A, S565G, Y567A | CA CALCIUM ION × 4 CL CHLORIDE ION × 1 TTP THYMIDINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；7% PEG350 monomethyl ether, 0.1M NA CACODYLATE, 0.14M CACL2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.50 Å R-free 0.267 |
| 3CFR Structure of the replicating complex of a POL Alpha family DNA Polymerase, ternary complex 2 提交 2008-03-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：L561A, S565G, Y567A | CA CALCIUM ION × 5 CL CHLORIDE ION × 1 TTP THYMIDINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；7% PEG350 monomethyl ether, 0.1M NA CACODYLATE, 0.1 M CA2CL2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.40 Å R-free 0.266 |
| 3CQ8 Ternary complex of the L415F mutant RB69 exo(-)polymerase 提交 2008-04-02 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
片段：RB69 polymerase
|
突变：L415F, D222A, D327A | CA CALCIUM ION × 6 NA SODIUM ION × 3 TTP THYMIDINE-5'-TRIPHOSPHATE × 1 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；220mM CaCl2, 25% monometheyl ether PEG350, and 50mM Tris-HCL, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
分辨率 2.50 Å R-free 0.281 |
| 3KD1 Closed binary complex of an RB69 gp43 fingers domain mutant complexed with an acyclic GMP terminated primer template pair. 提交 2009-10-22 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 E
1–903（903 aa）
片段：RB69 gp43 exo- chimera containing elements from the fingers domain of the human cytomegalovirus DNA polymerase.
|
突变：D222A V478W F479V N480S I557M N558A R559L L561V I562T I563C | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；5% PEG 20000, 0.1M sodium acetate pH 5, 0.1M magnesium acetate, 0.1M Tris-HCl pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.66 Å R-free 0.248 |
| 3KD5 Closed ternary complex of an RB69 gp43 fingers domain mutant complexed with an acyclic GMP terminated primer template pair and phosphonoformic acid. 提交 2009-10-22 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 E
1–903（903 aa）
片段：RB69 gp43 exo- chimera containing elements from the fingers domain of the human cytomegalovirus DNA polymerase.
|
突变：D222A V478W F479V N480S I557M N558A R559L L561V I562T I563C | PPF PHOSPHONOFORMIC ACID × 1 MG MAGNESIUM ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；5% PEG 20000, 0.1M sodium acetate pH5, 0.1M magnesium acetate, 0.1M Tris HCl pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.69 Å R-free 0.263 |
| 3L8B Crystal structure of a replicative DNA polymerase bound to the oxidized guanine lesion guanidinohydantoin 提交 2009-12-30 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；297 K；reservoir: 12% PEG2000MME, 100mM Sodium Acetate, 150mM Magnesium Sulfate,
100mM Hepes pH 7.0, 6% Glycerol and 2mM Beta-mercaptoethanol, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
分辨率 2.15 Å R-free 0.255 |
| 3L8B Crystal structure of a replicative DNA polymerase bound to the oxidized guanine lesion guanidinohydantoin 提交 2009-12-30 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A, D327A | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；297 K；reservoir: 12% PEG2000MME, 100mM Sodium Acetate, 150mM Magnesium Sulfate,
100mM Hepes pH 7.0, 6% Glycerol and 2mM Beta-mercaptoethanol, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
分辨率 2.15 Å R-free 0.255 |
| 3LDS Crystal structure of RB69 gp43 with DNA and dATP opposite 8-oxoG 提交 2010-01-13 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L561A, I563S | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 MN MANGANESE (II) ION × 3 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；297 K；3% PEG 20000, 100mM sodium acetate, 100mM manganese acetate, 100mM Tris-HCl, 1% glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
分辨率 3.00 Å R-free 0.295 |
| 3LZI RB69 DNA Polymerase (Y567A) ternary complex with dATP Opposite 7,8-dihydro-8-oxoguanine 提交 2010-03-01 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, Y567A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
micro-batch vapor-diffusion；pH 6.5；293.15 K；150 mM CaCl2, 11%(w/v) PEG 350 monomethyl ether MME, and 100 mM Na Cacodylate pH 6.5 , micro-batch vapor-diffusion, temperature 293.15K
|
分辨率 2.30 Å R-free 0.258 |
| 3LZJ RB69 DNA Polymerase (Y567A) ternary complex with dCTP Opposite 7,8-Dihydro-8-oxoguanine 提交 2010-03-01 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, Y567A | CTP CYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
micro-batch vapor-diffusion；pH 6.5；293.15 K；150 mM CaCl2, 14%(w/v) PEG 350 monomethyl ether (MME), and 100 mM Na Cacodylate (pH 6.5), micro-batch vapor-diffusion, temperature 293.15K
|
分辨率 2.05 Å R-free 0.251 |
| 3NAE RB69 DNA Polymerase (Y567A) Ternary Complex with dATP Opposite Guanidinohydantoin 提交 2010-06-01 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, Y567A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
micro-batch vapor-diffusion；pH 6.5；293 K；15%(w/v) PEG 350 monomethyl ether (MME), 150 mM CaCl2, and 100 mM NaCacodylate (pH 6.5), micro-batch vapor-diffusion, temperature 293K
|
分辨率 2.00 Å R-free 0.231 |
| 3NCI RB69 DNA Polymerase Ternary Complex with dCTP Opposite dG at 1.8 angstrom resolution 提交 2010-06-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
片段：DNA polymerase
|
突变：D222A, D327A | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
micro-batch vapor-diffusion；pH 6.5；293 K；8%(w/v) PEG 350 monomethyl ether (MME),160 mM CaCl2, and 100 mM NaCacodylate (pH 6.5), micro-batch vapor-diffusion, temperature 293K
|
分辨率 1.79 Å R-free 0.201 |
| 3NDK RB69 DNA Polymerase (Y567A) Ternary Complex with dCTP Opposite dG 提交 2010-06-07 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, Y567A | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
micro-batch vapor-diffusion；pH 6.5；293 K；10% (w/v) PEG 350 monomethyl ether (MME), 175 mM CaCl2, 100 mM NaCacodylate (pH 6.5), micro-batch vapor-diffusion, temperature 293K
|
分辨率 2.00 Å R-free 0.229 |
| 3NE6 RB69 DNA Polymerase (S565G/Y567A) Ternary Complex with dCTP Opposite dG 提交 2010-06-08 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, S565G, Y567A | CA CALCIUM ION × 5 DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；293 K；7% (w/v) PEG 350 monomethyl ether (MME), 175 mM CaCl2, 100 mM NaCacodylate (pH 6.5), micro-batch vapor-diffusion, temperature 293K
|
分辨率 2.00 Å R-free 0.226 |
| 3NGI RB69 DNA Polymerase (Y567A) Ternary Complex with dTTP Opposite dG 提交 2010-06-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, Y567A | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 8 | X-RAY DIFFRACTION |
X-ray结晶条件
micro-batch vapor-diffusion；pH 6.5；293 K；13%(w/v) PEG 350 monomethyl ether (MME), 150 mM CaCl2, 100 mM NaCacodylate (pH 6.5), micro-batch vapor-diffusion, temperature 293K
|
分辨率 1.89 Å R-free 0.226 |
| 3NHG RB69 DNA Polymerase (S565G/Y567A) Ternary Complex with dTTP Opposite dG 提交 2010-06-14 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, S565G, Y567A | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；293 K；apply 7% (w/v) PEG 350 monomethyl ether (MME), 175 mM CaCl2, and 100 mM NaCacodylate (pH 6.5) to crystal Y567A/S565G: P/T:dCTP ternary complex and then soak dTTP into the crystal to replace dCTP in the ternary complex, micro-batch vapor-diffusion, temperature 293K
|
分辨率 2.50 Å R-free 0.244 |
| 3QEI RB69 DNA Polymerase (L561A/S565G/Y567A) Ternary Complex with dCTP Opposite Difluorotoluene Nucleoside 提交 2011-01-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：L561A, S565G, Y567A | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5), VAPOR DIFFUSION, temperature 298K
|
分辨率 2.18 Å R-free 0.238 |
| 3QEP RB69 DNA Polymerase (L561A/S565G/Y567A) Ternary Complex with dTTP Opposite Difluorotoluene Nucleoside 提交 2011-01-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：L561A, S565G, Y567A | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5), VAPOR DIFFUSION, temperature 298K
|
分辨率 1.80 Å R-free 0.204 |
| 3QER RB69 DNA Polymerase (L561A/S565G/Y567A) Ternary Complex with dATP Opposite Difluorotoluene Nucleoside 提交 2011-01-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：L561A, S565G, Y567A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), 100 mM sodium cacodylate (pH 6.5), VAPOR DIFFUSION, temperature 298K
|
分辨率 1.96 Å R-free 0.217 |
| 3QES RB69 DNA Polymerase (L561A/S565G/Y567A) Ternary Complex with dGTP Opposite Difluorotoluene Nucleoside 提交 2011-01-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L561A, S565G, Y567A | DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5), VAPOR DIFFUSION, temperature 298K
|
分辨率 1.98 Å R-free 0.218 |
| 3QET RB69 DNA Polymerase (L561A/S565G/Y567A) Ternary Complex with dTTP Opposite dT 提交 2011-01-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L561A, S565G, Y567A | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5), VAPOR DIFFUSION, temperature 298K
|
分辨率 2.08 Å R-free 0.217 |
| 3QEV RB69 DNA Polymerase (L561A/S565G/Y567A) Ternary Complex with dCTP Opposite dT 提交 2011-01-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L561A, S565G, Y567A | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5), VAPOR DIFFUSION, temperature 298K
|
分辨率 1.77 Å R-free 0.210 |
| 3QEW RB69 DNA Polymerase (L561A/S565G/Y567A) Ternary Complex with dDTP Opposite dT 提交 2011-01-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L561A, S565G, Y567A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.84 Å R-free 0.205 |
| 3QEX RB69 DNA Polymerase (L561A/S565G/Y567A) Ternary Complex with dGTP Opposite dT 提交 2011-01-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L561A, S565G, Y567A | DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5) , VAPOR DIFFUSION, temperature 298K
|
分辨率 1.73 Å R-free 0.206 |
| 3QNN RB69 DNA Polymerase (Y567A) Ternary Complex with dGT Opposite 3tCo 提交 2011-02-08 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–901（901 aa）
|
突变：Y567A | DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 15%(w/v) PEG350 monomethyl ether (MME), and 100mM sodium cacodylate (pH6.5), VAPOR DIFFUSION, temperature 298K
|
分辨率 1.92 Å R-free 0.214 |
| 3QNO RB69 DNA Polymerase (Y567A) Ternary Complex with dATP Opposite 3tCo 提交 2011-02-08 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–901（901 aa）
|
突变：Y567A | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；298 K；150 mM CaCl2, 15%(w/v) PEG350 monomethyl ether (MME), and 100mM sodium cacodylate (pH6.5), VAPOR DIFFUSION, temperature 298K
|
分辨率 1.88 Å R-free 0.212 |
| 3RMA Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；297 K；10-11 % PEG 2000 MME, 100 mM Na acetate, 150 mM MgSO4, 100 mM Hepes, 6-10 % glycerol, 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 7.2, VAPOR DIFFUSION, HANGING DROP
|
分辨率 2.84 Å R-free 0.275 |
| 3RMA Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；297 K；10-11 % PEG 2000 MME, 100 mM Na acetate, 150 mM MgSO4, 100 mM Hepes, 6-10 % glycerol, 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 7.2, VAPOR DIFFUSION, HANGING DROP
|
分辨率 2.84 Å R-free 0.275 |
| 3RMA Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 3 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；297 K；10-11 % PEG 2000 MME, 100 mM Na acetate, 150 mM MgSO4, 100 mM Hepes, 6-10 % glycerol, 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 7.2, VAPOR DIFFUSION, HANGING DROP
|
分辨率 2.84 Å R-free 0.275 |
| 3RMA Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 4 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 D
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；297 K；10-11 % PEG 2000 MME, 100 mM Na acetate, 150 mM MgSO4, 100 mM Hepes, 6-10 % glycerol, 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 7.2, VAPOR DIFFUSION, HANGING DROP
|
分辨率 2.84 Å R-free 0.275 |
| 3RMB Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；297 K；6.5 % PEG 2000 MME, 100 mM Na acetate, 150 mM MgSO4, 100 mM Hepes pH 7.2, 6% glycerol and 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K
|
分辨率 2.65 Å R-free 0.276 |
| 3RMB Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；297 K；6.5 % PEG 2000 MME, 100 mM Na acetate, 150 mM MgSO4, 100 mM Hepes pH 7.2, 6% glycerol and 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K
|
分辨率 2.65 Å R-free 0.276 |
| 3RMB Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 3 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–903（903 aa）
|
突变：D222A, D327A | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；297 K；6.5 % PEG 2000 MME, 100 mM Na acetate, 150 mM MgSO4, 100 mM Hepes pH 7.2, 6% glycerol and 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K
|
分辨率 2.65 Å R-free 0.276 |
| 3RMB Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 4 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 D
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；297 K；6.5 % PEG 2000 MME, 100 mM Na acetate, 150 mM MgSO4, 100 mM Hepes pH 7.2, 6% glycerol and 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K
|
分辨率 2.65 Å R-free 0.276 |
| 3RMC Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.8；297 K；4.5 % PEG 20,000, 150 mM Na acetate, 125 mM Mg acetate, 100 mM TrisHCl, 1% glycerol, 10mM Phenol and 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 6.8, VAPOR DIFFUSION, HANGING DROP
|
分辨率 3.00 Å R-free 0.282 |
| 3RMC Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.8；297 K；4.5 % PEG 20,000, 150 mM Na acetate, 125 mM Mg acetate, 100 mM TrisHCl, 1% glycerol, 10mM Phenol and 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 6.8, VAPOR DIFFUSION, HANGING DROP
|
分辨率 3.00 Å R-free 0.282 |
| 3RMC Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 3 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.8；297 K；4.5 % PEG 20,000, 150 mM Na acetate, 125 mM Mg acetate, 100 mM TrisHCl, 1% glycerol, 10mM Phenol and 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 6.8, VAPOR DIFFUSION, HANGING DROP
|
分辨率 3.00 Å R-free 0.282 |
| 3RMC Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 4 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 D
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.8；297 K；4.5 % PEG 20,000, 150 mM Na acetate, 125 mM Mg acetate, 100 mM TrisHCl, 1% glycerol, 10mM Phenol and 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 6.8, VAPOR DIFFUSION, HANGING DROP
|
分辨率 3.00 Å R-free 0.282 |
| 3RMD Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.8；297 K；4% PEG 20,000, 100 mM Na acetate, 125 mM Mg acetate, 100 mM TrisHCl, 1% glycerol, 10mM Phenol, 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 6.8, VAPOR DIFFUSION, HANGING DROP
|
分辨率 2.98 Å R-free 0.275 |
| 3RMD Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A, D327A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.8；297 K；4% PEG 20,000, 100 mM Na acetate, 125 mM Mg acetate, 100 mM TrisHCl, 1% glycerol, 10mM Phenol, 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 6.8, VAPOR DIFFUSION, HANGING DROP
|
分辨率 2.98 Å R-free 0.275 |
| 3RMD Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 3 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–903（903 aa）
|
突变：D222A, D327A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.8；297 K；4% PEG 20,000, 100 mM Na acetate, 125 mM Mg acetate, 100 mM TrisHCl, 1% glycerol, 10mM Phenol, 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 6.8, VAPOR DIFFUSION, HANGING DROP
|
分辨率 2.98 Å R-free 0.275 |
| 3RMD Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol 提交 2011-04-20 | Assembly 4 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 D
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.8；297 K；4% PEG 20,000, 100 mM Na acetate, 125 mM Mg acetate, 100 mM TrisHCl, 1% glycerol, 10mM Phenol, 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 6.8, VAPOR DIFFUSION, HANGING DROP
|
分辨率 2.98 Å R-free 0.275 |
| 3RWU RB69 DNA Polymerase (Y567A) Ternary Complex with dATP Opposite Difluorotoluene Nucleoside 提交 2011-05-09 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–901（901 aa）
|
突变：Y567A | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 15% (w/v) PEG350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH6.5), VAPOR DIFFUSION, temperature 298K
|
分辨率 2.33 Å R-free 0.276 |
| 3S9H RB69 DNA Polymerase Triple Mutant(L561A/S565G/Y567A) ternary complex with dUpNpp and a dideoxy-terminated primer in the presence of Ca2+ 提交 2011-06-01 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A,L561A,S565G,Y567A | CA CALCIUM ION × 7 DUP 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
MICROBATCH VAPOR DIFFUSION；pH 6.5；293.15 K；150 mM calcium chloride, 1% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, MICROBATCH VAPOR DIFFUSION, temperature 293.15K
|
分辨率 1.95 Å R-free 0.213 |
| 3SCX RB69 DNA Polymerase Triple Mutant(L561A/S565G/Y567A) Ternary Complex with dUpNpp and a Deoxy-terminated Primer in the Presence of Ca2+ 提交 2011-06-08 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A,L561A,S565G,Y567A | DUP 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE × 1 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
MICROBATCH VAPOR DIFFUSION；pH 6.5；293.15 K；150 mM calcium chloride, 1% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, MICROBATCH VAPOR DIFFUSION, temperature 293.15K
|
分辨率 2.35 Å R-free 0.259 |
| 3SI6 RB69 DNA Polymerase Triple Mutant (L561A/S565G/Y567A) Ternary Complex with dUpNpp and a Deoxy-terminated Primer in the presence of Mg2+ 提交 2011-06-17 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L561A, S565G,Y567A | DUP 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
MICROBATCH VAPOR DIFFUSION；pH 6.5；293.15 K；150 mM calcium chloride, 1% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, MICROBATCH VAPOR DIFFUSION, temperature 293.15K
|
分辨率 1.85 Å R-free 0.217 |
| 3SJJ RB69 DNA Polymerase Triple Mutant (L561A/S565G/Y567A) Ternary Complex with dUpNpp and a Deoxy-terminated Primer in the presence of Mn2+ 提交 2011-06-21 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L561A, S565G,Y567A | DUP 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE × 1 MN MANGANESE (II) ION × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
MICROBATCH VAPOR DIFFUSION；pH 6.5；293.15 K；150 mM calcium chloride, 1% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, MICROBATCH VAPOR DIFFUSION, temperature 293.15K
|
分辨率 2.38 Å R-free 0.265 |
| 3SNN RB69 DNA Polymerase (L561A/S565G/Y567A) Ternary Complex with dCTP Opposite dG in the presence of Mg2+ 提交 2011-06-29 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L561A, S565G, Y567A | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
MICROBATCH VAPOR DIFFUSION；pH 6.5；293.15 K；150 mM calcium chloride, 1% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, soak crystals with the same buffer containing 100 mM magnesium chloride instead of calcium chloride, MICROBATCH VAPOR DIFFUSION, temperature 293.15K
|
分辨率 2.00 Å R-free 0.222 |
| 3SPY RB69 DNA Polymerase(L415A/L561A/S565G/Y567A) Ternary Complex with dUpCpp Opposite dA 提交 2011-07-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–901（901 aa）
|
突变：L415A, L561A, S565G, Y567A | UPC 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]uridine × 1 MG MAGNESIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM calcium chloride, 15% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.14 Å R-free 0.242 |
| 3SPZ DNA Polymerase(L415A/L561A/S565G/Y567A) Ternary Complex with dUpCpp Opposite dA (Ca2+) 提交 2011-07-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：L415A, L561A, S565G, Y567A | UPC 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]uridine × 1 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM calcium chloride, 15% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.43 Å R-free 0.257 |
| 3SQ0 DNA Polymerase(L561A/S565G/Y567A) Ternary Complex with dUpNpp Opposite dA (Mn2+) 提交 2011-07-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：L561A, S565G, Y567A | DUP 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE × 1 MN MANGANESE (II) ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM calcium chloride, 15% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.00 Å R-free 0.245 |
| 3SQ1 RB69 DNA Polymerase Ternary Complex with dUpCpp Opposite dA 提交 2011-07-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–901（901 aa）
|
突变：yes | DUP 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM calcium chloride, 15% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.82 Å R-free 0.213 |
| 3SQ2 RB69 DNA Polymerase Ternary Complex with dTTP Opposite 2AP (AT rich sequence) 提交 2011-07-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–902（902 aa）
|
突变：yes | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM calcium chloride, 15% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.10 Å R-free 0.244 |
| 3SQ4 RB69 DNA Polymerase Ternary Complex with dTTP Opposite 2AP (GC rich sequence) 提交 2011-07-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–902（902 aa）
|
突变：yes | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM calcium chloride, 15% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.23 Å R-free 0.238 |
| 3SUN RB69 DNA Polymerase (Y567A) Ternary Complex with dTTP Opposite 2AP (AT rich sequence) 提交 2011-07-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–895（895 aa）
|
突变：Y567A | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM calcium chloride, 15% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.42 Å R-free 0.255 |
| 3SUO RB69 DNA Polymerase (Y567A) Ternary Complex with dTTP Opposite 2AP (GC rich sequence) 提交 2011-07-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–900（900 aa）
|
突变：Y567A | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM calcium chloride, 15% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.23 Å R-free 0.255 |
| 3SUP RB69 DNA Polymerase (Y567A) Ternary Complex with dCTP Opposite 2AP (GC rich sequence) 提交 2011-07-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：Y567A | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM calcium chloride, 15% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.32 Å R-free 0.261 |
| 3SUQ RB69 DNA Polymerase (Y567A) Ternary Complex with dCTP Opposite 2AP (AT rich sequence) 提交 2011-07-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–897（897 aa）
|
突变：Y567A | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM calcium chloride, 15% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 3.15 Å R-free 0.300 |
| 3TAB 5-hydroxycytosine paired with dGMP in RB69 gp43 提交 2011-08-03 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% PEG 2000-MME, 100 mM NaOAc, 100 mM MgSO4, 100 mM Hepes, 5% glycerol, 1 mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 2.80 Å R-free 0.281 |
| 3TAB 5-hydroxycytosine paired with dGMP in RB69 gp43 提交 2011-08-03 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A, D327A | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% PEG 2000-MME, 100 mM NaOAc, 100 mM MgSO4, 100 mM Hepes, 5% glycerol, 1 mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 2.80 Å R-free 0.281 |
| 3TAB 5-hydroxycytosine paired with dGMP in RB69 gp43 提交 2011-08-03 | Assembly 3 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–903（903 aa）
|
突变：D222A, D327A | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% PEG 2000-MME, 100 mM NaOAc, 100 mM MgSO4, 100 mM Hepes, 5% glycerol, 1 mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 2.80 Å R-free 0.281 |
| 3TAB 5-hydroxycytosine paired with dGMP in RB69 gp43 提交 2011-08-03 | Assembly 4 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 D
1–903（903 aa）
|
突变：D222A, D327A | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% PEG 2000-MME, 100 mM NaOAc, 100 mM MgSO4, 100 mM Hepes, 5% glycerol, 1 mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 2.80 Å R-free 0.281 |
| 3TAE 5-hydroxycytosine paired with dAMP in RB69 gp43 提交 2011-08-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% Peg 2000-MME, 100mM NaOAc, 100mM MgSO4, 100mM Hepes, 5% glycerol, 1mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 2.71 Å R-free 0.268 |
| 3TAE 5-hydroxycytosine paired with dAMP in RB69 gp43 提交 2011-08-04 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–903（903 aa）
|
突变：D222A, D327A | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% Peg 2000-MME, 100mM NaOAc, 100mM MgSO4, 100mM Hepes, 5% glycerol, 1mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 2.71 Å R-free 0.268 |
| 3TAE 5-hydroxycytosine paired with dAMP in RB69 gp43 提交 2011-08-04 | Assembly 3 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A, D327A | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% Peg 2000-MME, 100mM NaOAc, 100mM MgSO4, 100mM Hepes, 5% glycerol, 1mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 2.71 Å R-free 0.268 |
| 3TAE 5-hydroxycytosine paired with dAMP in RB69 gp43 提交 2011-08-04 | Assembly 4 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 D
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% Peg 2000-MME, 100mM NaOAc, 100mM MgSO4, 100mM Hepes, 5% glycerol, 1mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 2.71 Å R-free 0.268 |
| 3TAF 5-fluorocytosine paired with ddGMP in RB69 gp43 提交 2011-08-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% Peg 2000-MME, 100mM NaOAc, 100mM MgSO4, 100mM Hepes, 5% glycerol, 1mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 3.00 Å R-free 0.280 |
| 3TAF 5-fluorocytosine paired with ddGMP in RB69 gp43 提交 2011-08-04 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A, D327A | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% Peg 2000-MME, 100mM NaOAc, 100mM MgSO4, 100mM Hepes, 5% glycerol, 1mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 3.00 Å R-free 0.280 |
| 3TAF 5-fluorocytosine paired with ddGMP in RB69 gp43 提交 2011-08-04 | Assembly 3 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–903（903 aa）
|
突变：D222A, D327A | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% Peg 2000-MME, 100mM NaOAc, 100mM MgSO4, 100mM Hepes, 5% glycerol, 1mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 3.00 Å R-free 0.280 |
| 3TAF 5-fluorocytosine paired with ddGMP in RB69 gp43 提交 2011-08-04 | Assembly 4 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 D
1–903（903 aa）
|
突变：D222A, D327A | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% Peg 2000-MME, 100mM NaOAc, 100mM MgSO4, 100mM Hepes, 5% glycerol, 1mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 3.00 Å R-free 0.280 |
| 3TAG 5-fluorocytosine paired with dAMP in RB69 gp43 提交 2011-08-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% Peg 2000-MME, 100mM NaOAc, 100mM MgSO4, 100mM Hepes, 5% glycerol, 1mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 2.95 Å R-free 0.284 |
| 3TAG 5-fluorocytosine paired with dAMP in RB69 gp43 提交 2011-08-04 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% Peg 2000-MME, 100mM NaOAc, 100mM MgSO4, 100mM Hepes, 5% glycerol, 1mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 2.95 Å R-free 0.284 |
| 3TAG 5-fluorocytosine paired with dAMP in RB69 gp43 提交 2011-08-04 | Assembly 3 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% Peg 2000-MME, 100mM NaOAc, 100mM MgSO4, 100mM Hepes, 5% glycerol, 1mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 2.95 Å R-free 0.284 |
| 3TAG 5-fluorocytosine paired with dAMP in RB69 gp43 提交 2011-08-04 | Assembly 4 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 D
1–903（903 aa）
|
突变：D222A, D327A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；7% Peg 2000-MME, 100mM NaOAc, 100mM MgSO4, 100mM Hepes, 5% glycerol, 1mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 2.95 Å R-free 0.284 |
| 3UIQ RB69 DNA Polymerase Ternary Complex containing dUpNpp 提交 2011-11-05 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
未记录 | DUP 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE × 1 CA CALCIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150mM CaCl2, 15% PEG 350 MME, 100mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.88 Å R-free 0.220 |
| 4DTJ RB69 DNA Polymerase Ternary Complex with dTTP Opposite an Abasic Site and ddT/dA as the Penultimate Base-pair 提交 2012-02-21 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–901（901 aa）
|
未记录 | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；100 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), 100 mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.90 Å R-free 0.202 |
| 4DTM RB69 DNA Polymerase Ternary Complex with dCTP Opposite an Abasic Site and ddG/dC as the Penultimate Base-pair 提交 2012-02-21 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–901（901 aa）
|
未记录 | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；100 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), 100 mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.95 Å R-free 0.220 |
| 4DTN RB69 DNA Polymerase Ternary Complex with dATP Opposite an Abasic Site and ddA/dT as the Penultimate Base-pair 提交 2012-02-21 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
未记录 | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；100 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), 100 mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.96 Å R-free 0.216 |
| 4DTO RB69 DNA Polymerase Ternary Complex with dCTP Opposite an Abasic Site and ddA/dT as the Penultimate Base-pair 提交 2012-02-21 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
未记录 | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；100 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), 100 mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.05 Å R-free 0.225 |
| 4DTP RB69 DNA Polymerase Ternary Complex with dGTP Opposite an Abasic Site and ddA/dT as the Penultimate Base-pair 提交 2012-02-21 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
未记录 | DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；100 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), 100 mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.05 Å R-free 0.229 |
| 4DTR RB69 DNA Polymerase Ternary Complex with dATP Opposite an Abasic Site and ddC/dG as the Penultimate Base-pair 提交 2012-02-21 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L561A, S565G, Y567A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；100 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), 100 mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.04 Å R-free 0.228 |
| 4DTS RB69 DNA Polymerase Ternary Complex with dCTP Opposite an Abasic Site and ddC/dG as the Penultimate Base-pair 提交 2012-02-21 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L561A, L565G, Y567A | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；100 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), 100 mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.96 Å R-free 0.206 |
| 4DTU RB69 DNA Polymerase Ternary Complex with dGTP Opposite an Abasic Site and ddC/dG as the Penultimate Base-pair 提交 2012-02-21 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L561A, S565G, Y567A | DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；100 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), 100 mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.86 Å R-free 0.201 |
| 4DTX RB69 DNA Polymerase Ternary Complex with dTTP Opposite an Abasic Site and ddC/dG as the Penultimate Base-pair 提交 2012-02-21 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L561A, S565G, Y567A | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；100 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), 100 mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.84 Å R-free 0.206 |
| 4DU1 RB69 DNA Polymerase Ternary Complex with dATP Opposite dT 提交 2012-02-21 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
未记录 | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；100 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), 100 mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.15 Å R-free 0.245 |
| 4DU3 RB69 DNA Polymerase Ternary Complex with dDTP Opposite dT with 3-Deaza-adenine at the N-1 Position of Template Strand 提交 2012-02-21 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
未记录 | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；100 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), 100 mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.02 Å R-free 0.227 |
| 4DU4 RB69 DNA Polymerase Ternary Complex with dATP Opposite dT with 3-Deaza-adenine at the N-3 Position of Primer Strand 提交 2012-02-21 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；100 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), 100 mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.28 Å R-free 0.249 |
| 4E3S RB69 DNA Polymerase Ternary Complex with dQTP Opposite dT 提交 2012-03-10 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：L561A, S565G, Y567A | QTP 3-{2-deoxy-5-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-erythro-pentofuranosyl}-7-methyl-3H-imidazo[4,5-b]pyridine × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150mM CaCl2, 15% PEG350 MME, 100mM sodium cacodylate (pH6.5), VAPOR DIFFUSION, temperature 298K
|
分辨率 2.04 Å R-free 0.219 |
| 4FJ5 RB69 DNA polymerase ternary complex with dATP/dT 提交 2012-06-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.05 Å R-free 0.238 |
| 4FJ7 RB69 DNA polymerase ternary complex with dGTP/dT 提交 2012-06-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.90 Å R-free 0.213 |
| 4FJ8 RB69 DNA polymerase ternary complex with dCTP/dT 提交 2012-06-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.19 Å R-free 0.252 |
| 4FJ9 RB69 DNA polymerase ternary complex with dTTP/dT 提交 2012-06-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), 100 mM sodium cacodylate pH 6.5 , VAPOR DIFFUSION, temperature 298K
|
分辨率 1.97 Å R-free 0.235 |
| 4FJG RB69 DNA polymerase ternary complex with dATP/dC 提交 2012-06-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5 , VAPOR DIFFUSION, temperature 298K
|
分辨率 2.02 Å R-free 0.264 |
| 4FJH RB69 DNA polymerase ternary complex with dGTP/dC 提交 2012-06-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.11 Å R-free 0.223 |
| 4FJI RB69 DNA polymerase ternary complex with dcTP/dC 提交 2012-06-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5 , VAPOR DIFFUSION, temperature 298K
|
分辨率 2.20 Å R-free 0.264 |
| 4FJJ RB69 DNA polymerase ternary complex with dTTP/dC 提交 2012-06-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5 , VAPOR DIFFUSION, temperature 298K
|
分辨率 1.99 Å R-free 0.225 |
| 4FJK RB69 DNA polymerase ternary complex with dATP/dA 提交 2012-06-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.00 Å R-free 0.226 |
| 4FJL RB69 DNA polymerase ternary complex with dGTP/dA 提交 2012-06-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.87 Å R-free 0.223 |
| 4FJM RB69 DNA polymerase ternary complex with dCTP/dA 提交 2012-06-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L561A, S565G, Y567A | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.02 Å R-free 0.232 |
| 4FJN RB69 DNA polymerase ternary complex with dTTP/dA 提交 2012-06-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5 , VAPOR DIFFUSION, temperature 298K
|
分辨率 1.98 Å R-free 0.240 |
| 4FJX RB69 DNA polymerase ternary complex with dATP/dG 提交 2012-06-12 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5 , VAPOR DIFFUSION, temperature 298K
|
分辨率 2.11 Å R-free 0.225 |
| 4FK0 RB69 DNA polymerase ternary complex with dCTP/dG 提交 2012-06-12 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.18 Å R-free 0.252 |
| 4FK2 RB69 DNA polymerase ternary complex with dTTP/dG 提交 2012-06-12 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.98 Å R-free 0.225 |
| 4FK4 RB69 DNA polymerase ternary complex with dGTP/dG 提交 2012-06-12 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, L415A, L561A, S565G, Y567A | DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.90 Å R-free 0.222 |
| 4I9L Crystal structure of the D714A mutant of RB69 DNA polymerase 提交 2012-12-05 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, D714A | GMP GUANOSINE × 1 SO4 SULFATE ION × 3 CL CHLORIDE ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；1.65 M AMMONIUM SULPHATE, 0.01 M MAGNESIUM CHLORIDE, 0.05 M SODIUM CITRATE DIHYDROGEN, PH 5.6, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K
|
分辨率 2.60 Å R-free 0.249 |
| 4I9Q Crystal structure of the ternary complex of the D714A mutant of RB69 DNA polymerase 提交 2012-12-05 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A D327A,Y567A,D714A | XG4 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]guanosine × 2 CA CALCIUM ION × 1 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 5.6；298 K；10% PEG 8000, 0.2 M (NH4)2SO4 and 0.1 M sodium citrate (pH 5.6), VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.30 Å R-free 0.287 |
| 4I9Q Crystal structure of the ternary complex of the D714A mutant of RB69 DNA polymerase 提交 2012-12-05 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A D327A,Y567A,D714A | XG4 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]guanosine × 2 CA CALCIUM ION × 1 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 5.6；298 K；10% PEG 8000, 0.2 M (NH4)2SO4 and 0.1 M sodium citrate (pH 5.6), VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.30 Å R-free 0.287 |
| 4J2A RB69 DNA Polymerase L415A Ternary Complex 提交 2013-02-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–901（901 aa）
|
突变：L415A, D222A, D327A | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 15% PEG350 monomethyl ether (MME), and 100 mM Sodium Carcodylate , pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.80 Å R-free 0.211 |
| 4J2B RB69 DNA Polymerase L415G Ternary Complex 提交 2013-02-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–901（901 aa）
片段：RB69 DNA polymerase
|
突变：L415G, D222A, D327A | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 15% PEG350 monomethyl ether (MME), and 100 mM Sodium Carcodylate (pH6.5) , VAPOR DIFFUSION, temperature 298K
|
分辨率 2.04 Å R-free 0.220 |
| 4J2D RB69 DNA Polymerase L415K Ternary Complex 提交 2013-02-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–901（901 aa）
|
突变：L415K, D222A, D327A | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 9 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 15% PEG350 monomethyl ether (MME), and 100 mM Sodium Cacodylate , pH 6.5, VAPOR DIFFUSION, temperature 298K
|
分辨率 1.76 Å R-free 0.197 |
| 4J2E RB69 DNA Polymerase L415M Ternary Complex 提交 2013-02-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–901（901 aa）
片段：RB69 DNA polymerase
|
突变：L415M, D222A, D327A | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 15% PEG350 monomethyl ether (MME), and 100 mM Sodium Carcodylate (pH6.5), VAPOR DIFFUSION, temperature 298K
|
分辨率 2.02 Å R-free 0.213 |
| 4KHN Crystal structure of the ternary complex of the D714A mutant of RB69 DNA polymerase 提交 2013-04-30 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A, D327A, Y567A, D714A | XG4 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]guanosine × 2 CA CALCIUM ION × 1 SO4 SULFATE ION × 5 GOL GLYCEROL × 2 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；12% PEG 8000, 0.1 M MES, 0.2 Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6
|
分辨率 2.55 Å R-free 0.211 |
| 4KHN Crystal structure of the ternary complex of the D714A mutant of RB69 DNA polymerase 提交 2013-04-30 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
1–903（903 aa）
|
突变：D222A, D327A, Y567A, D714A | XG4 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]guanosine × 2 CA CALCIUM ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；12% PEG 8000, 0.1 M MES, 0.2 Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6
|
分辨率 2.55 Å R-free 0.211 |
| 4KHQ Ternary complex of RB69 mutant L415F wit DUMPNPP 提交 2013-05-01 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D327A, D222A, L415F | CA CALCIUM ION × 3 DUP 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE × 1 NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；50 MM TRIS, PH 7.5, 10% PEG350, 180 MM CALCIUM CHLORIDE, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 295K, temperature 298K
|
分辨率 2.19 Å R-free 0.212 |
| 4KHS Ternary complex of RB69 mutant L415F with a ribonucleotide at 0 position 提交 2013-05-01 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D327A, D222A, L415F | CA CALCIUM ION × 3 TTP THYMIDINE-5'-TRIPHOSPHATE × 1 NA SODIUM ION × 4 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；50 MM TRIS-HCL, 10% PEG350, 220 MM CALCIUM CHLORIDE, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 295K, pH 7.5, temperature 298K
|
分辨率 2.12 Å R-free 0.216 |
| 4KHU Ternary complex of rb69 mutant L415F with a ribonucleotide at -1 position 提交 2013-05-01 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D327A, D222A, L415F | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 NA SODIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；50 MM TRIS-HCL, 13% PEG350, 180 MM CALCIUM CHLORIDE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 2.05 Å R-free 0.247 |
| 4KHW Ternary complex of RB69 mutant L415F with ribonucleotide at -2 position 提交 2013-05-01 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D327A, D222A, L415F | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 8 NA SODIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；50 MM TRIS-HCL, 19% PEG350, 180 MM CALCIUM CHLORIDE,, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 2.37 Å R-free 0.224 |
| 4KHY Ternary complex of rb69 mutant L415F with ribonucleotide at -3 position 提交 2013-05-01 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：YES | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 6 NA SODIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.5；298 K；50 MM TRIS-HCL, 17.5% PEG350, 180 MM CALCIUM CHLORIDE, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 295K, temperature 298K
|
分辨率 2.25 Å R-free 0.241 |
| 4KI4 Ternary complex of rb69 mutant L415F with ribonucleotides at 0 and -1 position 提交 2013-05-01 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D327A, D222A, L415F | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 4 NA SODIUM ION × 6 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；50 MM TRIS-HCL, 16% PEG350, 180 MM CALCIUM CHLORIDE , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 2.45 Å R-free 0.221 |
| 4KI6 Ternary complex of rb69 mutant l415f with ribonucleotides at -1 and -2 position 提交 2013-05-01 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D327A, D222A, L415F | TTP THYMIDINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 6 NA SODIUM ION × 2 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；50 MM TRIS-HCL, 16% PEG350, 180 MM CALCIUM CHLORIDE, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 2.55 Å R-free 0.233 |
| 4M3R RB69 DNA polymerase ternary complex with dT/dG at position n-1 of primer/template duplex 提交 2013-08-06 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A,L415A,L561A,S565G,Y567A | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 12% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5), VAPOR DIFFUSION, temperature 298K
|
分辨率 2.07 Å R-free 0.221 |
| 4M3T RB69 DNA polymerase ternary complex with dT/dG at position n-2 of primer/template duplex 提交 2013-08-06 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A,L415A,L561A,S565G,Y567A | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 12% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5) , VAPOR DIFFUSION, temperature 298K
|
分辨率 1.90 Å R-free 0.209 |
| 4M3U RB69 DNA polymerase ternary complex with dT/dG at position n-3 of primer/template duplex 提交 2013-08-06 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A,L415A,L561A,S565G,Y567A | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 12% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5) , VAPOR DIFFUSION, temperature 298K
|
分辨率 2.07 Å R-free 0.223 |
| 4M3W RB69 DNA polymerase ternary complex with dT/dG at position n-4 of primer/template duplex 提交 2013-08-06 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A,L415A,L561A,S565G,Y567A | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 12% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5) , VAPOR DIFFUSION, temperature 298K
|
分辨率 2.10 Å R-free 0.220 |
| 4M3X RB69 DNA polymerase ternary complex with dT/dG at position n-5 of primer/template duplex 提交 2013-08-06 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A,L415A,L561A,S565G,Y567A | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 12% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5) , VAPOR DIFFUSION, temperature 298K
|
分辨率 2.20 Å R-free 0.241 |
| 4M3Y RB69 DNA polymerase ternary complex with dG/dT at position n-1 of primer/template duplex 提交 2013-08-06 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A,L415A,L561A,S565G,Y567A | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 12% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5) , VAPOR DIFFUSION, temperature 298K
|
分辨率 1.86 Å R-free 0.221 |
| 4M3Z RB69 DNA polymerase ternary complex with dG/dT at position n-2 of primer/tempLate duplex 提交 2013-08-06 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A,L415A,L561A,S565G,Y567A | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 12% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5) , VAPOR DIFFUSION, temperature 298K
|
分辨率 1.84 Å R-free 0.207 |
| 4M41 RB69 DNA polymerase ternary complex with dG/dT at position n-3 of primer/tempLate duplex 提交 2013-08-06 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A,L415A,L561A,S565G,Y567A | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 12% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5) , VAPOR DIFFUSION, temperature 298K
|
分辨率 2.15 Å R-free 0.237 |
| 4M42 RB69 DNA polymerase ternary complex with dG/dT at position n-4 of primer/tempLate duplex 提交 2013-08-06 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A,L415A,L561A,S565G,Y567A | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 12% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5) , VAPOR DIFFUSION, temperature 298K
|
分辨率 2.04 Å R-free 0.224 |
| 4M45 RB69 DNA polymerase ternary complex with dG/dT at position n-5 of primer/template duplex 提交 2013-08-06 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–903（903 aa）
|
突变：D222A,D327A,L415A,L561A,S565G,Y567A | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 6.5；298 K；150 mM CaCl2, 12% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5) , VAPOR DIFFUSION, temperature 298K
|
分辨率 1.89 Å R-free 0.214 |
| 7F4Y Crystal structure of replisomal dimer of DNA polymerase from bacteriophage RB69 with DNA duplexes 提交 2021-06-21 | Assembly 1 蛋白–DNA 同源多聚体；蛋白 × 2 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 A
1–903（903 aa）
链 B
1–903（903 aa）
|
未记录 | DUP 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；294 K；sodium acetate, isopropanol, magnesium chloride, sodium chloride, spermine tetrahydrochloride
|
分辨率 2.20 Å R-free 0.245 |