Current Protein Identity:Q9WX76
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1EH1 RIBOSOME RECYCLING FACTOR FROM THERMUS THERMOPHILUS Deposited 2000-02-18 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–185(185 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 4.9;293 K;ammonium sulphate, sodium acetate, MES, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 20K
|
Resolution 2.60 Å R-free 0.307 |
| 3J0D Models for the T. thermophilus ribosome recycling factor bound to the E. coli post-termination complex Deposited 2011-06-29 | Assembly 1 Protein–RNA Heteromer;Protein × 3 PDB declaration: undecameric(11) Consistent with all polymers |
Chain J
1–185(185 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
BUFFER R;pH 7.5;BUFFER R
cryo-EM vitrification conditions
Cryogen ETHANE;VITROBOT
|
Resolution 11.10 Å |
| 3J0E Models for the T. thermophilus ribosome recycling factor and the E. coli elongation factor G bound to the E. coli post-termination complex Deposited 2011-06-29 | Assembly 1 Protein–RNA Heteromer;Protein × 3 PDB declaration: nonameric(9) Consistent with all polymers |
Chain G
1–185(185 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
BUFFER R;pH 7.5;BUFFER R
cryo-EM vitrification conditions
Cryogen ETHANE;VITROBOT
|
Resolution 9.90 Å |
| 4V54 Crystal structure of the bacterial ribosome from Escherichia coli in complex with ribosome recycling factor (RRF). Deposited 2007-06-16 | Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 53-meric(53) Consistent with all polymers |
Chain B6
1–185(185 aa)
|
Not recorded | MG MAGNESIUM ION × 170 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
batch;pH 7.5;277 K;MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K
|
Resolution 3.30 Å R-free 0.304 |
| 4V54 Crystal structure of the bacterial ribosome from Escherichia coli in complex with ribosome recycling factor (RRF). Deposited 2007-06-16 | Assembly 2 Protein–RNA Heteromer;Protein × 50 PDB declaration: 53-meric(53) Consistent with all polymers |
Chain D6
1–185(185 aa)
|
Not recorded | MG MAGNESIUM ION × 173 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
batch;pH 7.5;277 K;MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K
|
Resolution 3.30 Å R-free 0.304 |
| 4V55 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin and ribosome recycling factor (RRF). Deposited 2007-06-17 | Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 53-meric(53) Consistent with all polymers |
Chain B6
1–185(185 aa)
|
Not recorded | MG MAGNESIUM ION × 170 LLL (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL × 2 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
batch;pH 7.5;277 K;MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K
|
Resolution 4.00 Å R-free 0.305 |
| 4V55 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin and ribosome recycling factor (RRF). Deposited 2007-06-17 | Assembly 2 Protein–RNA Heteromer;Protein × 50 PDB declaration: 53-meric(53) Consistent with all polymers |
Chain D6
1–185(185 aa)
|
Not recorded | MG MAGNESIUM ION × 173 LLL (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL × 2 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
batch;pH 7.5;277 K;MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K
|
Resolution 4.00 Å R-free 0.305 |
| 4V5A Structure of the Ribosome Recycling Factor bound to the Thermus thermophilus 70S ribosome with mRNA, ASL-Phe and tRNA-fMet Deposited 2007-06-28 | Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 56-meric(56) Consistent with all polymers |
Chain AY
1–185(185 aa)
|
Not recorded | MG MAGNESIUM ION × 741 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7;COMPARE SELMER ET AL SCIENCE 2006, pH 7.0
|
Resolution 3.50 Å R-free 0.326 |
| 4V5A Structure of the Ribosome Recycling Factor bound to the Thermus thermophilus 70S ribosome with mRNA, ASL-Phe and tRNA-fMet Deposited 2007-06-28 | Assembly 2 Protein–RNA Heteromer;Protein × 50 PDB declaration: 56-meric(56) Consistent with all polymers |
Chain CY
1–185(185 aa)
|
Not recorded | MG MAGNESIUM ION × 741 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7;COMPARE SELMER ET AL SCIENCE 2006, pH 7.0
|
Resolution 3.50 Å R-free 0.326 |
| 4V5Y Crystal structure of the bacterial ribosome from Escherichia coli in complex with paromomycin and ribosome recycling factor (RRF). Deposited 2007-06-19 | Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 53-meric(53) Consistent with all polymers |
Chain B6
1–185(185 aa)
|
Not recorded | MG MAGNESIUM ION × 170 PAR PAROMOMYCIN × 2 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
batch;pH 7.5;277 K;MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K
|
Resolution 4.45 Å R-free 0.309 |
| 4V5Y Crystal structure of the bacterial ribosome from Escherichia coli in complex with paromomycin and ribosome recycling factor (RRF). Deposited 2007-06-19 | Assembly 2 Protein–RNA Heteromer;Protein × 50 PDB declaration: 53-meric(53) Consistent with all polymers |
Chain D6
1–185(185 aa)
|
Not recorded | MG MAGNESIUM ION × 173 PAR PAROMOMYCIN × 2 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
batch;pH 7.5;277 K;MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K
|
Resolution 4.45 Å R-free 0.309 |
| 6UCQ Crystal structure of the Thermus thermophilus 70S ribosome recycling complex Deposited 2019-09-17 | Assembly 1 Insufficient information Heteromer;Protein × 50 PDB declaration: 56-meric(56) Consistent with all polymers |
Chain 1w
1–185(185 aa)
|
Not recorded | MG MAGNESIUM ION × 1523 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION;pH 7.3;292 K;0.15 M L-arginine-HCl, 0.1M Tris-HCl pH 7.3, 2.4-2.7% PEG-20K, 7-9% MPD, 0.5 mM BME
|
Resolution 3.50 Å R-free 0.294 |
| 6UCQ Crystal structure of the Thermus thermophilus 70S ribosome recycling complex Deposited 2019-09-17 | Assembly 2 Insufficient information Heteromer;Protein × 50 PDB declaration: 56-meric(56) Consistent with all polymers |
Chain 2w
1–185(185 aa)
|
Not recorded | MG MAGNESIUM ION × 1350 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION;pH 7.3;292 K;0.15 M L-arginine-HCl, 0.1M Tris-HCl pH 7.3, 2.4-2.7% PEG-20K, 7-9% MPD, 0.5 mM BME
|
Resolution 3.50 Å R-free 0.294 |