SAXSdb — 1agr

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1. Structure Basics

entry_id	1agr
deposition_date	1997-03-25
title	COMPLEX OF ALF4-ACTIVATED GI-ALPHA-1 WITH RGS4
keywords	;GI-ALPHA-1, HYDROLASE, SIGNAL TRANSDUCTION, RGS4, COMPLEX (SIGNAL TRANSDUCTION-REGULATOR), GTP-BINDING, GTPASE ACTIVATING PROTEIN, COMPLEX (SIGNAL TRANSDUCTION-REGULATOR) complex ;; COMPLEX (SIGNAL TRANSDUCTION/REGULATOR)
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	34.91 Å
rg_electron	34.75 Å
i0	187820000.00
molecular_weight	109410.0 kDa
excluded_volume	136560 Å³
envelope_volume	175630 Å³
shell_volume	42478 Å³
envelope_diameter	132.4 Å
shell_rg	40.52 Å
envelope_rg	35.24 Å
shape_rg	34.72 Å
total_rg	35.23 Å
total_atoms	7674
n_residues	938
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	122.7 Å
rg_real	34.99 Å
rg_real_error	1.08 Å
i0_real	1.8780e+08
i0_real_error	3.0340e+06
rg_reciprocal	34.94 Å
i0_reciprocal	187800000.0000
total_estimate	0.8673
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	35.4 Å
skewness	0.401
kurtosis	-0.331
angular_range	— – 0.2250 Å⁻¹
current_alpha	0.0000
highest_alpha	26510000.0000
n_real_points	46
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.793; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.937; Smooth: 0.953

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4. Crystallography & Experiment

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5. Entities & Polymer Info (7)

Entity 1 polymer

description	GUANINE NUCLEOTIDE-BINDING PROTEIN G(I)
formula_weight	40267.8 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,D
atom_count	7732

GCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDAARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	RGS4
formula_weight	23286.7 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	E,H

MCKGLAGLPASCLRSAKDMKHRLGFLLQKSDSCEHSSSHSKKDKVVTCQRVSQEEVKKWAESLENLINHECGLAAFKAFLKSEYSEENIDFWISCEEYKKIKSPSKLSPKAKKIYNEFISVQATKEVNLDSCTREETSRNMLEPTITCFDEAQKKIFNLMEKDSYRRFLKSRFYLDLTNPSSCGAEKQKGAKSSADCTSLVPQCA

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli BL21(DE3)

Entity 3 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

Entity 4 non-polymer

description	TETRAFLUOROALUMINATE ION
formula_weight	103.0 kDa
src_method	syn
entity_count	2

Entity 5 non-polymer

description	GUANOSINE-5'-DIPHOSPHATE
formula_weight	443.2 kDa
src_method	syn
entity_count	2

Entity 6 non-polymer

description	CITRIC ACID
formula_weight	192.1 kDa
src_method	syn
entity_count	2

Entity 7 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	58

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6. Citations (1)

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length_a	164.000 Å
length_b	97.200 Å
length_c	110.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 21 21 2
int_tables_number	18
crystal_system	—

resolution	2.800 Å
resolution_low	5.000 Å
r_factor	0.2160
r_work	0.2160
r_free	0.2910

fit	/app/data/saxs_computation/ag/1agr/1agr.fit
pddf	/app/data/pr_computation/ag/1agr/1agr.pr.out
profile	/app/data/saxs_computation/ag/1agr/1agr.dat

COMPLEX OF ALF4-ACTIVATED GI-ALPHA-1 WITH RGS4

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (7)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 non-polymer

Entity 6 non-polymer

Entity 7 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)