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1as2

GDP+PI BOUND G42V GIA1

Method: X-RAY DIFFRACTION D_max: 70.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1as2
deposition_date	1997-08-11
title	GDP+PI BOUND G42V GIA1
keywords	SIGNAL TRANSDUCTION, GTPASE; SIGNAL TRANSDUCTION
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	21.64 Å
rg_electron	20.82 Å
i0	23431800.00
molecular_weight	36635.0 kDa
excluded_volume	45731 Å³
envelope_volume	53859 Å³
shell_volume	21783 Å³
envelope_diameter	71.2 Å
shell_rg	27.35 Å
envelope_rg	21.02 Å
shape_rg	20.84 Å
total_rg	21.63 Å
total_atoms	2571
n_residues	315
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	70.8 Å
rg_real	21.61 Å
rg_real_error	0.38 Å
i0_real	2.3430e+07
i0_real_error	2.8160e+05
rg_reciprocal	21.62 Å
i0_reciprocal	23430000.0000
total_estimate	0.8893
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	25.4 Å
skewness	0.348
kurtosis	-0.259
angular_range	— – 0.3650 Å⁻¹
current_alpha	0.0000
highest_alpha	4614000.0000
n_real_points	68
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.856; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.992; Smooth: 0.998

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	GIA1
formula_weight	40309.9 kDa
mutation	G42V
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2576

GCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAVESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDAARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	PHOSPHATE ION
formula_weight	95.0 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	GUANOSINE-5'-DIPHOSPHATE
formula_weight	443.2 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	5

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6. Citations (1)

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length_a	77.190 Å
length_b	77.190 Å
length_c	143.610 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 43 21 2
int_tables_number	96
crystal_system	—

resolution	2.800 Å
resolution_low	8.000 Å
r_factor	0.1890
r_work	0.1890
r_free	0.2500

fit	/app/data/saxs_computation/as/1as2/1as2.fit
pddf	/app/data/pr_computation/as/1as2/1as2.pr.out
profile	/app/data/saxs_computation/as/1as2/1as2.dat

GDP+PI BOUND G42V GIA1

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction