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1as3

GDP BOUND G42V GIA1

Method: X-RAY DIFFRACTION D_max: 82.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1as3
deposition_date	1997-08-11
title	GDP BOUND G42V GIA1
keywords	SIGNAL TRANSDUCTION, GTPASE; SIGNAL TRANSDUCTION
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	23.26 Å
rg_electron	22.39 Å
i0	25440600.00
molecular_weight	38118.0 kDa
excluded_volume	47572 Å³
envelope_volume	56839 Å³
shell_volume	21956 Å³
envelope_diameter	83.9 Å
shell_rg	28.62 Å
envelope_rg	22.86 Å
shape_rg	22.38 Å
total_rg	23.21 Å
total_atoms	2673
n_residues	330
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	82.7 Å
rg_real	23.34 Å
rg_real_error	0.72 Å
i0_real	2.5440e+07
i0_real_error	3.7210e+05
rg_reciprocal	23.32 Å
i0_reciprocal	25440000.0000
total_estimate	0.8494
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	25.4 Å
skewness	0.480
kurtosis	-0.114
angular_range	— – 0.3400 Å⁻¹
current_alpha	0.0000
highest_alpha	4589000.0000
n_real_points	66
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.735; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.892; Smooth: 0.941

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	GIA1
formula_weight	40309.9 kDa
mutation	G42V
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2737

GCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAVESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDAARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	GUANOSINE-5'-DIPHOSPHATE
formula_weight	443.2 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	64

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6. Citations (1)

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length_a	121.440 Å
length_b	121.440 Å
length_c	68.290 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	I 4
int_tables_number	79
crystal_system	—

resolution	2.400 Å
resolution_low	8.000 Å
r_factor	0.2120
r_work	0.2120
r_free	0.2740

fit	/app/data/saxs_computation/as/1as3/1as3.fit
pddf	/app/data/pr_computation/as/1as3/1as3.pr.out
profile	/app/data/saxs_computation/as/1as3/1as3.dat

GDP BOUND G42V GIA1

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction