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1as0

GTP-GAMMA-S BOUND G42V GIA1

Method: X-RAY DIFFRACTION D_max: 71.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1as0
deposition_date	1997-08-11
title	GTP-GAMMA-S BOUND G42V GIA1
keywords	SIGNAL TRANSDUCTION, GTPASE; SIGNAL TRANSDUCTION
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	21.23 Å
rg_electron	20.45 Å
i0	23496900.00
molecular_weight	36513.0 kDa
excluded_volume	45517 Å³
envelope_volume	52557 Å³
shell_volume	21557 Å³
envelope_diameter	71.8 Å
shell_rg	26.90 Å
envelope_rg	20.74 Å
shape_rg	20.47 Å
total_rg	21.21 Å
total_atoms	2559
n_residues	313
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	71.1 Å
rg_real	21.20 Å
rg_real_error	0.42 Å
i0_real	2.3500e+07
i0_real_error	2.8710e+05
rg_reciprocal	21.20 Å
i0_reciprocal	23500000.0000
total_estimate	0.8746
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	25.1 Å
skewness	0.350
kurtosis	-0.217
angular_range	— – 0.3750 Å⁻¹
current_alpha	0.0000
highest_alpha	5257000.0000
n_real_points	69
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.811; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.992; Smooth: 0.941

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	GIA1
formula_weight	40309.9 kDa
mutation	G42V
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2649

GCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAVESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDAARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	1

Entity 4 non-polymer

description	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE
formula_weight	539.2 kDa
src_method	syn
entity_count	1

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	90

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6. Citations (1)

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length_a	78.950 Å
length_b	78.950 Å
length_c	105.290 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 32 2 1
int_tables_number	154
crystal_system	—

resolution	2.000 Å
resolution_low	8.000 Å
r_factor	0.2060
r_work	0.2060
r_free	0.2590

fit	/app/data/saxs_computation/as/1as0/1as0.fit
pddf	/app/data/pr_computation/as/1as0/1as0.pr.out
profile	/app/data/saxs_computation/as/1as0/1as0.dat