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1akd

CYTOCHROME P450CAM FROM PSEUDOMONAS PUTIDA, COMPLEXED WITH 1S-CAMPHOR

Method: X-RAY DIFFRACTION D_max: 70.3 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1akd
deposition_date	1997-05-16
title	CYTOCHROME P450CAM FROM PSEUDOMONAS PUTIDA, COMPLEXED WITH 1S-CAMPHOR
keywords	OXIDOREDUCTASE, OXYGENASE, CYTOCHROME P450, MONOOXYGENASE, ELECTRON TRANSPORT; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	22.66 Å
rg_electron	21.43 Å
i0	35924800.00
molecular_weight	46384.0 kDa
excluded_volume	58093 Å³
envelope_volume	67371 Å³
shell_volume	25736 Å³
envelope_diameter	71.4 Å
shell_rg	28.75 Å
envelope_rg	21.65 Å
shape_rg	21.44 Å
total_rg	22.30 Å
total_atoms	3263
n_residues	405
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	70.3 Å
rg_real	22.55 Å
rg_real_error	0.28 Å
i0_real	3.5920e+07
i0_real_error	4.5170e+05
rg_reciprocal	22.58 Å
i0_reciprocal	35930000.0000
total_estimate	0.9024
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	27.7 Å
skewness	0.177
kurtosis	-0.476
angular_range	— – 0.3500 Å⁻¹
current_alpha	0.0000
highest_alpha	7921000.0000
n_real_points	67
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.921; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.990; Smooth: 0.975

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	CYTOCHROME P450CAM
formula_weight	46587.9 kDa
ec_number	1.14.15.1
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	3545

TTETIQSNANLAPLPPHVPEHLVFDFDMYNPSNLSAGVQEAWAVLQESNVPDLVWTRCNGGHWIATRGQLIREAYEDYRHFSSECPFIPREAGEAYDFIPTSMDPPEQRQFRALANQVVGMPVVDKLENRIQELACSLIESLRPQGQCNFTEDYAEPFPIRIFMLLAGLPEEDIPHLKYLTDQMTRPDGSMTFAEAKEALYDYLIPIIEQRRQKPGTDAISIVANGQVNGRPITSDEAKRMCGLLLVGGLDTVVNFLSFSMEFLAKSPEHRQELIQRPERIPAACEELLRRFSLVADGRILTSDYEFHGVQLKKGDQILLPQMLSGLDERENACPMHVDFSRQKVSHTTFGHGSHLCLGQHLARREIIVTLKEWLTRIPDFSIAPGAQIQHKSGIVSGVQALPLVWDPATTKAV

Source

organism	Pseudomonas putida
ncbi_taxonomy_id	303
host_organism	Escherichia coli

Entity 2 non-polymer

description	POTASSIUM ION
formula_weight	39.1 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	PROTOPORPHYRIN IX CONTAINING FE
formula_weight	616.5 kDa
src_method	syn
entity_count	1

Entity 4 non-polymer

description	CAMPHOR
formula_weight	152.2 kDa
src_method	syn
entity_count	1

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	271

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6. Citations (2)

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length_a	64.300 Å
length_b	66.200 Å
length_c	106.800 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	1.800 Å
resolution_low	26.000 Å
r_factor	0.2090
r_work	0.2090
r_free	0.2650

fit	/app/data/saxs_computation/ak/1akd/1akd.fit
pddf	/app/data/pr_computation/ak/1akd/1akd.pr.out
profile	/app/data/saxs_computation/ak/1akd/1akd.dat