CYTOCHROME P450-CAM
Pseudomonas putida
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 1–414 | Mutation：F87W/Y96F | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 8000, Cacodylate, Sodium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | Resolution 2.10 Å R-free 0.264 |
| 2 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain B； UniProt 1–414 | Mutation：F87W/Y96F | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 8000, Cacodylate, Sodium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | Resolution 2.10 Å R-free 0.264 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 1C8J | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1AKD CYTOCHROME P450CAM FROM PSEUDOMONAS PUTIDA, COMPLEXED WITH 1S-CAMPHOR Deposited 1997-05-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
dialysis；pH 7.4；PROTEIN WAS CRYSTALLIZED BY DIALYSIS FROM 10% PEG 8000, 50 MM TRISHCL PH 7.4, 250 MM KCL, 100 MM DTE, IN THE PRESENCE OF EXCESS (1S)-CAMPHOR., dialysis
|
Resolution 1.80 Å R-free 0.265 |
| 1CP4 FORMATION, CRYSTAL STRUCTURE, AND REARRANGEMENT OF A CYTOCHROME P450-CAM IRON-PHENYL COMPLEX Deposited 1991-06-04 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 BNZ BENZENE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 1DZ4 ferric p450cam from pseudomonas putida Deposited 2000-02-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；275 K；CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40
|
Resolution 1.60 Å R-free 0.237 |
| 1DZ4 ferric p450cam from pseudomonas putida Deposited 2000-02-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；275 K；CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40
|
Resolution 1.60 Å R-free 0.237 |
| 1DZ6 ferrous p450cam from pseudomonas putida Deposited 2000-02-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；275 K；CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40
|
Resolution 1.90 Å R-free 0.268 |
| 1DZ6 ferrous p450cam from pseudomonas putida Deposited 2000-02-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；275 K；CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40
|
Resolution 1.90 Å R-free 0.268 |
| 1DZ8 oxygen complex of p450cam from pseudomonas putida Deposited 2000-02-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 OXY OXYGEN MOLECULE × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；275 K；CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40
|
Resolution 1.90 Å R-free 0.248 |
| 1DZ8 oxygen complex of p450cam from pseudomonas putida Deposited 2000-02-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；275 K；CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40
|
Resolution 1.90 Å R-free 0.248 |
| 1DZ9 Putative oxo complex of P450cam from Pseudomonas putida Deposited 2000-02-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 O OXYGEN ATOM × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；275 K；CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40
|
Resolution 1.90 Å R-free 0.248 |
| 1DZ9 Putative oxo complex of P450cam from Pseudomonas putida Deposited 2000-02-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；275 K；CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40
|
Resolution 1.90 Å R-free 0.248 |
| 1GEB X-RAY CRYSTAL STRUCTURE AND CATALYTIC PROPERTIES OF THR252ILE MUTANT OF CYTOCHROME P450CAM Deposited 2000-11-01 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：T252I | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
SMALL TUBES；pH 6.5；298 K；(NH4)2SO4, DTT, pH 6.5, SMALL TUBES, temperature 298K
|
Resolution 2.03 Å R-free 0.228 |
| 1GEK STRUCTURAL CHARACTERIZATION OF N-BUTYL-ISOCYANIDE COMPLEXES OF CYTOCHROMES P450NOR AND P450CAM Deposited 2000-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NBN N-BUTYL ISOCYANIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
SMALL TUBES；pH 6；298 K；(NH4)2SO4, pH 6.0, SMALL TUBES, temperature 298K
|
Resolution 1.70 Å R-free 0.271 |
| 1GEM STRUCTURAL CHARACTERIZATION OF N-BUTYL-ISOCYANIDE COMPLEXES OF CYTOCHROMES P450NOR AND P450CAM Deposited 2000-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NBN N-BUTYL ISOCYANIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
SMALL TUBES；pH 6；298 K；(NH4)2SO4, pH 6.0, SMALL TUBES, temperature 298K
|
Resolution 2.00 Å R-free 0.225 |
| 1GJM Covalent attachment of an electroactive sulphydryl reagent in the active site of cytochrome P450cam Deposited 2001-07-26 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Mutation：YES | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 FEM N-(2-FERROCENYLETHYL)MALEIMIDE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；SEE MAIN REFERENCE, pH 7.50
|
Resolution 2.20 Å R-free 0.221 |
| 1IWI Putidaredoxin-Binding Stablilizes an Active Conformer of Cytochrome P450cam in its Reduced State; Crystal Structure of Cytochrome P450cam Deposited 2002-05-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
0–414（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
SMALL TUBES；pH 6；293 K；ammonium sulfate, pH 6.0, SMALL TUBES, temperature 293K
|
Resolution 2.00 Å R-free 0.182 |
| 1IWJ Putidaredoxin-Binding Stablilizes an Active Conformer of Cytochrome P450cam in its Reduced State; Crystal Structure of Mutant(109K) Cytochrome P450cam Deposited 2002-05-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
0–414（415 aa）
|
Mutation：R109K | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
SMALL TUBES；pH 6；293 K；ammonium sulfate, pH 6.0, SMALL TUBES, temperature 293K
|
Resolution 2.00 Å R-free 0.223 |
| 1IWK Putidaredoxin-Binding Stablilizes an Active Conformer of Cytochrome P450cam in its Reduced State; Crystal Structure of Mutant(112K) Cytochrome P450cam Deposited 2002-05-15 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
0–414（415 aa）
|
Mutation：R112K | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
SMALL TUBES；pH 6；293 K；ammonium sulfate, pH 6.0, SMALL TUBES, temperature 293K
|
Resolution 2.00 Å R-free 0.235 |
| 1J51 CRYSTAL STRUCTURE OF CYTOCHROME P450CAM MUTANT (F87W/Y96F/V247L/C334A) WITH 1,3,5-TRICHLOROBENZENE Deposited 2002-01-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Mutation：F87W/Y96F/V247L/C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 TCZ 1,3,5-TRICHLORO-BENZENE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 6.5；291 K；PEG8000, sodium acetate, sodium cacodylate, pH 6.5, EVAPORATION, temperature 291K
|
Resolution 2.20 Å R-free 0.258 |
| 1J51 CRYSTAL STRUCTURE OF CYTOCHROME P450CAM MUTANT (F87W/Y96F/V247L/C334A) WITH 1,3,5-TRICHLOROBENZENE Deposited 2002-01-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–414（414 aa）
|
Mutation：F87W/Y96F/V247L/C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 TCZ 1,3,5-TRICHLORO-BENZENE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 6.5；291 K；PEG8000, sodium acetate, sodium cacodylate, pH 6.5, EVAPORATION, temperature 291K
|
Resolution 2.20 Å R-free 0.258 |
| 1J51 CRYSTAL STRUCTURE OF CYTOCHROME P450CAM MUTANT (F87W/Y96F/V247L/C334A) WITH 1,3,5-TRICHLOROBENZENE Deposited 2002-01-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–414（414 aa）
|
Mutation：F87W/Y96F/V247L/C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 TCZ 1,3,5-TRICHLORO-BENZENE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 6.5；291 K；PEG8000, sodium acetate, sodium cacodylate, pH 6.5, EVAPORATION, temperature 291K
|
Resolution 2.20 Å R-free 0.258 |
| 1J51 CRYSTAL STRUCTURE OF CYTOCHROME P450CAM MUTANT (F87W/Y96F/V247L/C334A) WITH 1,3,5-TRICHLOROBENZENE Deposited 2002-01-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
1–414（414 aa）
|
Mutation：F87W/Y96F/V247L/C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 6.5；291 K；PEG8000, sodium acetate, sodium cacodylate, pH 6.5, EVAPORATION, temperature 291K
|
Resolution 2.20 Å R-free 0.258 |
| 1K2O Cytochrome P450Cam with Bound BIS(2,2'-BIPYRIDINE)-(5-METHYL-2-2'-BIPYRIDINE)-C2-ADAMANTANE RUTHENIUM (II) Deposited 2001-09-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Mutation：C334A | CAC CACODYLATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RFA DELTA-BIS(2,2'-BIPYRIDINE)-(5-METHYL-2-2'-BIPYRIDINE)-C2-ADAMANTANE RUTHENIUM (II) × 1 RFB LAMBDA-BIS(2,2'-BIPYRIDINE)-(5-METHYL-2-2'-BIPYRIDINE)-C2-ADAMANTANE RUTHENIUM (II) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
SLOW COOLING；pH 7.5；277 K；Hepes, PEG, KCL, DTT, pH 7.5, slow cooling, temperature 277K
|
Resolution 1.65 Å R-free 0.226 |
| 1K2O Cytochrome P450Cam with Bound BIS(2,2'-BIPYRIDINE)-(5-METHYL-2-2'-BIPYRIDINE)-C2-ADAMANTANE RUTHENIUM (II) Deposited 2001-09-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–414（414 aa）
|
Mutation：C334A | CAC CACODYLATE ION × 3 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RFA DELTA-BIS(2,2'-BIPYRIDINE)-(5-METHYL-2-2'-BIPYRIDINE)-C2-ADAMANTANE RUTHENIUM (II) × 1 RFB LAMBDA-BIS(2,2'-BIPYRIDINE)-(5-METHYL-2-2'-BIPYRIDINE)-C2-ADAMANTANE RUTHENIUM (II) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
SLOW COOLING；pH 7.5；277 K；Hepes, PEG, KCL, DTT, pH 7.5, slow cooling, temperature 277K
|
Resolution 1.65 Å R-free 0.226 |
| 1LWL Crystal Structure of Cytochrome P450-cam with a Fluorescent Probe D-8-Ad (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-octyl-amide) Deposited 2002-05-31 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 DSO ADAMANTANE-1-CARBOXYLIC ACID-5-DIMETHYLAMINO-NAPHTHALENE-1-SULFONYLAMINO-OCTYL-AMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；Citrate, PEG, KCl, DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.247 |
| 1MPW Molecular Recognition in (+)-a-Pinene Oxidation by Cytochrome P450cam Deposited 2002-09-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Mutation：F87W/Y96F/V247L/C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 TMH (+)-alpha-Pinene × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 6.5；291 K；PEG8000, sodium acetate, sodium cacodylate, pH 6.5, EVAPORATION, temperature 291K
|
Resolution 2.34 Å R-free 0.269 |
| 1MPW Molecular Recognition in (+)-a-Pinene Oxidation by Cytochrome P450cam Deposited 2002-09-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–414（414 aa）
|
Mutation：F87W/Y96F/V247L/C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 TMH (+)-alpha-Pinene × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 6.5；291 K；PEG8000, sodium acetate, sodium cacodylate, pH 6.5, EVAPORATION, temperature 291K
|
Resolution 2.34 Å R-free 0.269 |
| 1NOO CYTOCHROME P450-CAM COMPLEXED WITH 5-EXO-HYDROXYCAMPHOR Deposited 1995-12-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAH 5-EXO-HYDROXYCAMPHOR × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1O76 CYANIDE COMPLEX OF P450CAM FROM PSEUDOMONAS PUTIDA Deposited 2002-10-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CYN CYANIDE ION × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；275 K；CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40
|
Resolution 1.80 Å R-free 0.236 |
| 1O76 CYANIDE COMPLEX OF P450CAM FROM PSEUDOMONAS PUTIDA Deposited 2002-10-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CYN CYANIDE ION × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；275 K；CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40
|
Resolution 1.80 Å R-free 0.236 |
| 1P2Y CRYSTAL STRUCTURE OF CYTOCHROME P450CAM IN COMPLEX WITH (S)-(-)-NICOTINE Deposited 2003-04-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NCT (S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；PEG 8000, potassium phosphate, potassium chloride, dithiothreitol, nicotine, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.263 |
| 1P7R CRYSTAL STRUCTURE OF REDUCED, CO-EXPOSED COMPLEX OF CYTOCHROME P450CAM WITH (S)-(-)-NICOTINE Deposited 2003-05-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NCT (S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；PEG 8000, potassium phosphate, potassium chloride, dithiothreitol, nicotine, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.85 Å R-free 0.284 |
| 1PHA INHIBITOR-INDUCED CONFORMATIONAL CHANGE IN CYTOCHROME P450-CAM Deposited 1992-07-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 PFZ 1-(N-IMIDAZOLYL)-2-HYDROXY-2-(2,3-DICHLOROPHENYL)OCTANE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.63 Å |
| 1PHB INHIBITOR-INDUCED CONFORMATIONAL CHANGE IN CYTOCHROME P450-CAM Deposited 1992-07-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 PFZ 1-(N-IMIDAZOLYL)-2-HYDROXY-2-(2,3-DICHLOROPHENYL)OCTANE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.60 Å |
| 1PHC CRYSTAL STRUCTURE OF SUBSTRATE-FREE PSEUDOMONAS PUTIDA CYTOCHROME P450 Deposited 1992-07-27 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.60 Å |
| 1PHD CRYSTAL STRUCTURES OF METYRAPONE-AND PHENYLIMIDAZOLE-INHIBITED COMPLEXES OF CYTOCHROME P450-CAM Deposited 1992-07-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 PIW 1-phenyl-1H-imidazole × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.60 Å |
| 1PHE CRYSTAL STRUCTURES OF METYRAPONE-AND PHENYLIMIDAZOLE-INHIBITED COMPLEXES OF CYTOCHROME P450-CAM Deposited 1992-07-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | SO4 SULFATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 PIY 2-phenyl-1H-imidazole × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.60 Å |
| 1PHF CRYSTAL STRUCTURES OF METYRAPONE-AND PHENYLIMIDAZOLE-INHIBITED COMPLEXES OF CYTOCHROME P450-CAM Deposited 1992-07-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 PIM 4-PHENYL-1H-IMIDAZOLE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.60 Å |
| 1PHG CRYSTAL STRUCTURES OF METYRAPONE-AND PHENYLIMIDAZOLE-INHIBITED COMPLEXES OF CYTOCHROME P450-CAM Deposited 1992-07-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 MYT METYRAPONE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.60 Å |
| 1QMQ Optical detection of cytochrome P450 by sensitizer-linked substrates Deposited 1999-10-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Mutation：YES | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 DRB DELTA-BIS(2,2'-BIPYRIDINE)-(5-METHYL-2-2'-BIPYRIDINE)-C9-ADAMANTANE RUTHENIUM (II) × 2 ACT ACETATE ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6；pH 6.00
|
Resolution 1.55 Å R-free 0.226 |
| 1RE9 CRYSTAL STRUCTURE OF CYTOCHROME P450-CAM WITH A FLUORESCENT PROBE D-8-AD (ADAMANTANE-1-CARBOXYLIC ACID-5-DIMETHYLAMINO-NAPHTHALENE-1-SULFONYLAMINO-OCTYL-AMIDE) Deposited 2003-11-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 DSO ADAMANTANE-1-CARBOXYLIC ACID-5-DIMETHYLAMINO-NAPHTHALENE-1-SULFONYLAMINO-OCTYL-AMIDE × 1 EDO 1,2-ETHANEDIOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；CITRATE, PEG, KCL, DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.45 Å R-free 0.218 |
| 1RF9 Crystal structure of cytochrome P450-cam with a fluorescent probe D-4-AD (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-butyl-amide) Deposited 2003-11-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
0–414（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 DBR ADAMANTANE-1-CARBOXYLIC ACID-5-DIMETHYLAMINO-NAPHTHALENE-1-SULFONYLAMINO-BUTYL-AMIDE × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；CITRATE, PEG, KCL, DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.227 |
| 1T85 Crystal Structure of the Ferrous CO-bound Cytochrome P450cam Mutant (L358P/C334A) Deposited 2004-05-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Mutation：L358P,C334A | K POTASSIUM ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CMO CARBON MONOXIDE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.218 |
| 1T86 Crystal Structure of the Ferrous Cytochrome P450cam Mutant (L358P/C334A) Deposited 2004-05-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Mutation：L358P/C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；290 K；PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 290K
|
Resolution 1.90 Å R-free 0.236 |
| 1T86 Crystal Structure of the Ferrous Cytochrome P450cam Mutant (L358P/C334A) Deposited 2004-05-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–414（414 aa）
|
Mutation：L358P/C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；290 K；PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 290K
|
Resolution 1.90 Å R-free 0.236 |
| 1T87 Crystal Structure of the Ferrous CO-bound Cytochrome P450cam (C334A) Deposited 2004-05-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Mutation：C334A | K POTASSIUM ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；290 K；PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 290K
|
Resolution 1.80 Å R-free 0.207 |
| 1T87 Crystal Structure of the Ferrous CO-bound Cytochrome P450cam (C334A) Deposited 2004-05-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–414（414 aa）
|
Mutation：C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 CMO CARBON MONOXIDE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；290 K；PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 290K
|
Resolution 1.80 Å R-free 0.207 |
| 1T88 Crystal Structure of the Ferrous Cytochrome P450cam (C334A) Deposited 2004-05-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Mutation：C334A | K POTASSIUM ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；290 K；PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 290K
|
Resolution 1.90 Å R-free 0.212 |
| 1T88 Crystal Structure of the Ferrous Cytochrome P450cam (C334A) Deposited 2004-05-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–414（414 aa）
|
Mutation：C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；290 K；PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 290K
|
Resolution 1.90 Å R-free 0.212 |
| 1UYU Xenon COMPLEX OF wildtype P450CAM FROM PSEUDOMONAS PUTIDA Deposited 2004-03-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 XE XENON × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；275 K；CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40
|
Resolution 2.00 Å R-free 0.236 |
| 1UYU Xenon COMPLEX OF wildtype P450CAM FROM PSEUDOMONAS PUTIDA Deposited 2004-03-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 2 XE XENON × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；275 K；CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40
|
Resolution 2.00 Å R-free 0.236 |
| 1YRC X-ray Crystal Structure of hydrogenated Cytochrome P450cam Deposited 2005-02-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICRODIALYSIS；pH 7.4；277 K；TRISHCl, KCl, Camphor, DTE, PEG 8000, pH 7.4, MICRODIALYSIS, temperature 277K
|
Resolution 1.40 Å R-free 0.219 |
| 1YRD X-ray crystal structure of PERDEUTERATED Cytochrome P450cam Deposited 2005-02-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICRODIALYSIS；pH 7.4；277 K；TRISHCl, KCl, Camphor, DTE, PEG 8000, pH 7.4, MICRODIALYSIS, temperature 277K
|
Resolution 1.70 Å R-free 0.213 |
| 25BV Crystal structure of P450cam mutant-F87R Deposited 2026-03-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：F88R | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M ammonium acetate, 0.04 M citric acid,0.06 M bis-tris, pH6.4, 24% PEG3350
|
Resolution 1.74 Å R-free 0.210 |
| 25BV Crystal structure of P450cam mutant-F87R Deposited 2026-03-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–415（415 aa）
|
Mutation：F88R | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M ammonium acetate, 0.04 M citric acid,0.06 M bis-tris, pH6.4, 24% PEG3350
|
Resolution 1.74 Å R-free 0.210 |
| 2A1M Crystal structure of ferrous dioxygen complex of wild-type cytochrome P450cam Deposited 2005-06-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
0–414（415 aa）
|
Mutation：C334A | K POTASSIUM ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 OXY OXYGEN MOLECULE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；288 K；PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 288K
|
Resolution 2.10 Å R-free 0.247 |
| 2A1M Crystal structure of ferrous dioxygen complex of wild-type cytochrome P450cam Deposited 2005-06-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
0–414（415 aa）
|
Mutation：C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 OXY OXYGEN MOLECULE × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；288 K；PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 288K
|
Resolution 2.10 Å R-free 0.247 |
| 2A1N Crystal structure of ferrous dioxygen complex of D251N cytochrome P450cam Deposited 2005-06-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
0–414（415 aa）
|
Mutation：D251N/C334A | K POTASSIUM ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 OXY OXYGEN MOLECULE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；288 K；PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 288K
|
Resolution 1.90 Å R-free 0.235 |
| 2A1N Crystal structure of ferrous dioxygen complex of D251N cytochrome P450cam Deposited 2005-06-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
0–414（415 aa）
|
Mutation：D251N/C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 OXY OXYGEN MOLECULE × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；288 K；PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 288K
|
Resolution 1.90 Å R-free 0.235 |
| 2A1O Crystal structure of ferrous dioxygen complex of T252A cytochrome P450cam Deposited 2005-06-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
0–414（415 aa）
|
Mutation：T252A/C334A | K POTASSIUM ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 OXY OXYGEN MOLECULE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；288 K；PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 288K
|
Resolution 1.55 Å R-free 0.219 |
| 2A1O Crystal structure of ferrous dioxygen complex of T252A cytochrome P450cam Deposited 2005-06-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
0–414（415 aa）
|
Mutation：T252A/C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 OXY OXYGEN MOLECULE × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；288 K；PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 288K
|
Resolution 1.55 Å R-free 0.219 |
| 2CP4 CRYSTAL STRUCTURE OF THE CYTOCHROME P450-CAM ACTIVE SITE MUTANT THR252ALA Deposited 1991-06-04 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.10 Å |
| 2CPP HIGH-RESOLUTION CRYSTAL STRUCTURE OF CYTOCHROME P450-CAM Deposited 1987-04-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.63 Å |
| 2FE6 P450CAM from Pseudomonas putida reconstituted with manganic protoporphyrin IX Deposited 2005-12-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | K POTASSIUM ION × 1 MNR PROTOPORPHYRIN IX CONTAINING MN × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；7.5 MG/ML P450, 50 MM TRIS, 125 MM KCL, 13.5-15% PEG8000, 50 MM DTE, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.50 Å R-free 0.249 |
| 2FER P450CAM from Pseudomonas putida reconstituted with manganic protoporphyrin IX Deposited 2005-12-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
11–415（405 aa）
|
Not recorded | K POTASSIUM ION × 1 MNR PROTOPORPHYRIN IX CONTAINING MN × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；7.5 MG/ML P450, 25 MM POTASSIUM PHOSPHATE, 250 MM KCL, 50 MM TRIS, 13.5-15% PEG 8000, 50 MM DTE, pH 7.40, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.225 |
| 2FEU P450CAM from Pseudomonas putida reconstituted with manganic protoporphyrin IX Deposited 2005-12-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
11–415（405 aa）
|
Not recorded | K POTASSIUM ION × 1 MNR PROTOPORPHYRIN IX CONTAINING MN × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；7.5 MG/ML P450, 25 MM POTASSIUM PHOSPHATE, 250 MM KCL, 100 UM CAMPHOR, 50 MM TRIS, 13.5-15% PEG8000, 100 UM CAMPHOR, 50 MM DTE, pH 7.40, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.238 |
| 2FEU P450CAM from Pseudomonas putida reconstituted with manganic protoporphyrin IX Deposited 2005-12-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
11–415（405 aa）
|
Not recorded | K POTASSIUM ION × 2 MNR PROTOPORPHYRIN IX CONTAINING MN × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；7.5 MG/ML P450, 25 MM POTASSIUM PHOSPHATE, 250 MM KCL, 100 UM CAMPHOR, 50 MM TRIS, 13.5-15% PEG8000, 100 UM CAMPHOR, 50 MM DTE, pH 7.40, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.238 |
| 2FRZ Crystal Structure of Cytochrome P450cam mutant (F87W/Y96F/V247L/C334A) Deposited 2006-01-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Mutation：F87W, Y96F, V247L, C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；0.2M Sodium Acetate trihydrate, 0.1M Sodium Cacodylate, 25% PEG 8000, 0.01M spermidine , pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.10 Å R-free 0.243 |
| 2FRZ Crystal Structure of Cytochrome P450cam mutant (F87W/Y96F/V247L/C334A) Deposited 2006-01-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–414（414 aa）
|
Mutation：F87W, Y96F, V247L, C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；0.2M Sodium Acetate trihydrate, 0.1M Sodium Cacodylate, 25% PEG 8000, 0.01M spermidine , pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.10 Å R-free 0.243 |
| 2GQX Crystal structure of cytochrome p450cam mutant (f87w/y96f/l244a/v247l/c334a) with pentachlorobenzene Deposited 2006-04-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
10–414（405 aa）
|
Mutation：F87W/Y96F/L244A/V247L/C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 5CL 1,2,3,4,5-PENTACHLOROBENZENE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；0.2M Sodium Acetate trihydrate, 0.1M Sodium Cacodylate, 30% PEG 8000, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.10 Å R-free 0.236 |
| 2GQX Crystal structure of cytochrome p450cam mutant (f87w/y96f/l244a/v247l/c334a) with pentachlorobenzene Deposited 2006-04-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
10–414（405 aa）
|
Mutation：F87W/Y96F/L244A/V247L/C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；0.2M Sodium Acetate trihydrate, 0.1M Sodium Cacodylate, 30% PEG 8000, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.10 Å R-free 0.236 |
| 2GR6 Crystal structure of cytochrome p450cam mutant (f87w/y96f/l244a/v247l/c334a) Deposited 2006-04-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
10–414（405 aa）
|
Mutation：F87W/Y96F/L244A/V247L/C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；0.2M Sodium Acetate trihydrate, 0.1M Sodium Cacodylate, 25% PEG 8000, 13mM spermidine , pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.30 Å R-free 0.244 |
| 2GR6 Crystal structure of cytochrome p450cam mutant (f87w/y96f/l244a/v247l/c334a) Deposited 2006-04-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
10–414（405 aa）
|
Mutation：F87W/Y96F/L244A/V247L/C334A | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；0.2M Sodium Acetate trihydrate, 0.1M Sodium Cacodylate, 25% PEG 8000, 13mM spermidine , pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.30 Å R-free 0.244 |
| 2H7Q Cytochrome P450cam complexed with imidazole Deposited 2006-06-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 IMD IMIDAZOLE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；295 K；50mM Potassium phosphate pH 7.4, 250mM KCL, 50mM dithiothreitol, 36-52% ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.50 Å R-free 0.239 |
| 2H7R L244A mutant of Cytochrome P450cam complexed with imidazole Deposited 2006-06-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Mutation：L244A, C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 1MZ 1-METHYLIMIDAZOLE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；295 K；50mM Potassium phosphate, pH 7.4, 250mM KCL, 50 mM dithiothreitol, 36-52% ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.10 Å R-free 0.283 |
| 2H7S L244A mutant of Cytochrome P450cam Deposited 2006-06-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Mutation：L244A, C334A | HEC HEME C × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；286 K；50mM Potassium phosphate pH 7.4, 250mM KCL, 50mM dithiothreitol, 36-52% ammonium sulfate., VAPOR DIFFUSION, HANGING DROP, temperature 286K
|
Resolution 2.15 Å R-free 0.249 |
| 2H7S L244A mutant of Cytochrome P450cam Deposited 2006-06-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–414（414 aa）
|
Mutation：L244A, C334A | HEC HEME C × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；286 K；50mM Potassium phosphate pH 7.4, 250mM KCL, 50mM dithiothreitol, 36-52% ammonium sulfate., VAPOR DIFFUSION, HANGING DROP, temperature 286K
|
Resolution 2.15 Å R-free 0.249 |
| 2L8M Reduced and CO-bound cytochrome P450cam (CYP101A1) Deposited 2011-01-19 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
Fragment：sequence database residues 11-415
|
Not recorded | CAM CAMPHOR × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CMO CARBON MONOXIDE × 1 K POTASSIUM ION × 4 CL CHLORIDE ION × 3 |
SOLUTION NMR
NMR measurement conditions
pH 7.4；298 K；Ionic strength (raw mmCIF value) 100；Pressure ambient
NMR sample composition
0.4 mM [U-100% 13C; U-100% 15N; U-80% 2H] reduced-CO-bound CYP101A1, 3 mM camphor, pH 7.4 TrisHCl buffer, 100 mM KCl cytochrome, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.4 mM [U-15N] reduced-CO-bound CYP101A1, 3 mM camphor, pH 7.4 TrisHCl buffer, 100 mM KCl, 5% nematic phase C12E5/hexanol added for alignment, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.4 mM [U-15N] reduced-CO-bound CYP101A1, 3 mM camphor, pH 7.4 TrisHCl buffer, 100 mM KCl, except 8 mg/mL pf1 phage added, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2LQD Reduced and CO-bound cytochrome P450cam (CYP101A1) Deposited 2012-03-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–415（413 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CMO CARBON MONOXIDE × 1 K POTASSIUM ION × 1 |
SOLUTION NMR
NMR measurement conditions
pH 7.4；298 K；Ionic strength (raw mmCIF value) 100；Pressure 1
NMR sample composition
0.4 mM [U-100% 13C; U-100% 15N; U-80% 2H] cytochrome P450cam, 90 % H2O, 10 % D2O, 0.4 mM CYP101A1, 3 mM CAM, 100 mM KCl, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.4 mM [U-15N] cytochrome P450cam, 90 % H2O, 10 % D2O, 0.4 mM CYP101A1, 3 mM CAM, 100 mM KCl, 5 % C12E5/hexanol, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.4 mM [U-15N] cytochrome P450cam, 90 % H2O, 10 % D2O, 0.4 mM CYP101A1, 3 mM CAM, 100 mM KCl, 8 mg/mL pf1, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2M56 The structure of the complex of cytochrome P450cam and its electron donor putidaredoxin determined by paramagnetic NMR spectroscopy Deposited 2013-02-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
12–415（404 aa）
Fragment：UNP RESIDUES 12-415
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
SOLUTION NMR
NMR measurement conditions
pH 7.4；290 K；Ionic strength (raw mmCIF value) 100；Pressure ambient
NMR sample composition
100 uM [U-95% 15N] entity_1-1, 93% H2O/7% D2O | 93% H2O/7% D2O
|
Resolution not provided |
| 2QBL Crystal structure of ferric G248T cytochrome P450cam Deposited 2007-06-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：G248T | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；1 UL OF 15 MG/ML P450 IN 50 MM Potassium phosphate, 250 MM KCL WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION
(100 mM Tris, 250 mM KCl, 27-30% PEG 8000, 100 MM DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.264 |
| 2QBM Crystal structure of the P450cam G248T mutant in the cyanide bound state Deposited 2007-06-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：G248T | CYN CYANIDE ION × 1 K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；1 UL OF 15 MG/ML P450 IN 50 MM Potassium phosphate, 250 MM KCL WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION
(100 mM Tris, 250 mM KCl, 27-30% PEG 8000, 100 MM DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.220 |
| 2QBN Crystal structure of ferric G248V cytochrome P450cam Deposited 2007-06-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：G248V | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；1 UL OF 15 MG/ML P450 IN 50 MM Potassium phosphate, 250 MM KCL WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION
(100 mM Tris, 250 mM KCl, 27-30% PEG 8000, 100 MM DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.75 Å R-free 0.223 |
| 2QBO Crystal structure of the P450cam G248V mutant in the cyanide bound state Deposited 2007-06-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：G248V | CYN CYANIDE ION × 1 K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；1 UL OF 15 MG/ML P450 IN 50 MM Potassium phosphate, 250 MM KCL WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION
(100 mM Tris, 250 mM KCl, 27-30% PEG 8000, 100 MM DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.252 |
| 2Z97 Crystal Structure of Ferric Cytochrome P450cam Reconstituted with 7-Methyl-7-depropionated Hemin Deposited 2007-09-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | K POTASSIUM ION × 1 7HE 7-METHYL-7-DEPROPIONATEHEMIN × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；14% PEG 8000, 50mM Tris-HCl, 250mM KCl, 1mM d-camphor, 10mM dithioerythritol(DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.203 |
| 2ZAW Crystal Structure of Ferric Cytochrome P450cam Reconstituted with 6-Methyl-6-depropionated Hemin Deposited 2007-10-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | K POTASSIUM ION × 1 CL CHLORIDE ION × 1 6HE 6-METHY-6-DEPROPIONATEHEMIN × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；14% PEG 8000, 50mM Tris-HCl, 250mM KCl, 1mM d-camphor, 10mM dithioerythritol(DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.55 Å R-free 0.180 |
| 2ZAX Crystal Structure of Ferric Cytochrome P450cam Deposited 2007-10-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；11-14% PEG 4000, 50mM Tris-HCl, 250mM KCl, 1mM d-camphor, 10mM dithioerythritol(DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.60 Å R-free 0.183 |
| 2ZUH Crystal Structure of Camphor-soaked Ferric Cytochrome P450cam Mutant (D297A) Deposited 2008-10-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：D297A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；12-15% PEG 4000, 50mM Tris-HCl, 250mM KCl, 0.1mM d-Camphor, 10mM dithioerythritol(DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.55 Å R-free 0.185 |
| 2ZUI Crystal Structure of Camphor-soaked Ferric Cytochrome P450cam Mutant (D297N) Deposited 2008-10-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：D297N | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；12-15% PEG 4000, 50mM Tris-HCl, 250mM KCl, 1mM d-Camphor, 10mM dithioerythritol(DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.50 Å R-free 0.178 |
| 2ZUJ Crystal Structure of Camphor-soaked Ferric Cytochrome P450cam Mutant(D297L) Deposited 2008-10-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：D297L | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；11-14% PEG 8000, 250mM potassium chloride, 10mM DTE, 1mM d-camphor, 50mM Tris-HCl, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.60 Å R-free 0.203 |
| 2ZWT Crystal Structure of Ferric Cytochrome P450cam Deposited 2008-12-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；11-14% PEG 4000, 50mM Tris-HCl, 250mM KCl, 1mM d-camphor, 10mM dithioerythritol(DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.35 Å R-free 0.190 |
| 2ZWU Crystal Structure of Camphor Soaked Ferric Cytochrome P450cam Deposited 2008-12-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；11-14% PEG 4000, 50mM Tris-HCl, 250mM KCl, 1mM d-camphor, 10mM dithioerythritol(DTE); After crystallization, soaked camphor saturated buffer for 2 days, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.30 Å R-free 0.184 |
| 3CP4 CRYSTAL STRUCTURE OF THE CYTOCHROME P450-CAM ACTIVE SITE MUTANT THR252ALA Deposited 1991-06-04 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ADM ADAMANTANE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å |
| 3CPP CRYSTAL STRUCTURE OF THE CARBON MONOXY-SUBSTRATE-CYTOCHROME P450-CAM TERNARY COMPLEX Deposited 1989-07-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CMO CARBON MONOXIDE × 1 CAM CAMPHOR × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 3FWF Ferric camphor bound cytochrome P450cam containing a Selenocysteine as the 5th heme ligand, monoclinic crystal form Deposited 2009-01-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
11–415（405 aa）
|
Mutation：C357U, R365L, E366Q | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；24 % PEG 4000, 250 mM KCl, 50 mM TrisHCl, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.83 Å R-free 0.207 |
| 3FWF Ferric camphor bound cytochrome P450cam containing a Selenocysteine as the 5th heme ligand, monoclinic crystal form Deposited 2009-01-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
11–415（405 aa）
|
Mutation：C357U, R365L, E366Q | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；24 % PEG 4000, 250 mM KCl, 50 mM TrisHCl, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.83 Å R-free 0.207 |
| 3FWG Ferric camphor bound Cytochrome P450cam, Arg365Leu, Glu366Gln, monoclinic crystal form Deposited 2009-01-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
11–415（405 aa）
|
Mutation：R365L, E366Q | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；24% PEG 4000, 50 mM Tris HCl, 250 mM KCl, 20% glycerol, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.55 Å R-free 0.217 |
| 3FWG Ferric camphor bound Cytochrome P450cam, Arg365Leu, Glu366Gln, monoclinic crystal form Deposited 2009-01-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
11–415（405 aa）
|
Mutation：R365L, E366Q | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；24% PEG 4000, 50 mM Tris HCl, 250 mM KCl, 20% glycerol, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.55 Å R-free 0.217 |
| 3FWI Ferric camphor bound Cytochrome P450cam containing a selenocysteine as the 5th heme ligand, tetragonal crystal form Deposited 2009-01-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
11–415（405 aa）
|
Mutation：C357U, R365L, E366Q | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；24 % PEG 4000, 250 mM KCl, 50 mM Tris HCl, cryoprotection by addition of 20 % glycerol, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.40 Å R-free 0.238 |
| 3FWJ Ferric camphor bound Cytochrome P450cam containing a selenocysteine as the 5th heme ligand, orthorombic crystal form Deposited 2009-01-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
11–415（405 aa）
|
Mutation：C357U, R365L, E366Q | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；24% PEG 4000, 50 mM Tris HCl, 250 mM KCl, 20 % glycerol for cryoprotection, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.231 |
| 3L61 Crystal structure of substrate-free P450cam at 200 mM [K+] Deposited 2009-12-22 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；279 K；12-22% PEG 8000, 0.05 M Tris, 200 mM KCl, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 1.50 Å R-free 0.216 |
| 3L62 Crystal structure of substrate-free P450cam at low [K+] Deposited 2009-12-22 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；279 K；12-22% PEG 8000, 0.05 M Tris, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 1.70 Å R-free 0.224 |
| 3L63 Crystal structure of camphor-bound P450cam at low [K+] Deposited 2009-12-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；279 K；12-22% PEG 8000, 0.05M Tris, 4-8mM camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 1.50 Å R-free 0.197 |
| 3OIA Crystal Structure of Cytochrome P450cam crystallized in the presence of a tethered substrate analog AdaC1-C8GluEtg-Bio Deposited 2010-08-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
Fragment：Full length
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 A31 N-{15-oxo-19-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-4,7,10-trioxa-14-azanonadec-1-yl}-N'-(8-{[(3S,5S,7S)-tricyclo[3.3.1.1~3,7~]dec-1-ylcarbonyl]amino}octyl)pentanediamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 6.5；279 K；12-22% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.1-0.2M KCl, sitting-drop vapor diffusion, temperature 279K
|
Resolution 1.65 Å R-free 0.234 |
| 3OL5 Crystal Structure of Cytochrome P450cam crystallized with a tethered substrate analog 3OH-AdaC1-C8-Dans Deposited 2010-08-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
Fragment：Full length
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 SAW (1R,3S,5R,7S)-N-[8-({[5-(dimethylamino)naphthalen-1-yl]sulfonyl}amino)octyl]-3-hydroxytricyclo[3.3.1.1~3,7~]decane-1-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 6.5；279 K；12-22% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.1-0.2M KCl, sitting-drop vapor diffusion, temperature 279K
|
Resolution 1.75 Å R-free 0.219 |
| 3P6M Crystal Structure of Cytochrome P450cam crystallized in the presence of a tethered substrate analog AdaC1-C8-Dans Deposited 2010-10-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 DSO ADAMANTANE-1-CARBOXYLIC ACID-5-DIMETHYLAMINO-NAPHTHALENE-1-SULFONYLAMINO-OCTYL-AMIDE × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 6.5；279 K；12-22% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.1-0.2M KCl, sitting-drop vapor diffusion, temperature 279K
|
Resolution 2.00 Å R-free 0.251 |
| 3P6N Crystal Structure of Cytochrome P450cam crystallized in the presence of a tethered substrate analog AdaC1-C8-Dans Deposited 2010-10-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 DSO ADAMANTANE-1-CARBOXYLIC ACID-5-DIMETHYLAMINO-NAPHTHALENE-1-SULFONYLAMINO-OCTYL-AMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 6.5；279 K；12-22% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.1-0.2M KCl, sitting-drop vapor diffusion, temperature 279K
|
Resolution 1.70 Å R-free 0.227 |
| 3P6O Crystal Structure of Cytochrome P450cam crystallized in the presence of a tethered substrate analog AdaC1-Etg-Dans Deposited 2010-10-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 ETG (3S,5S,7S)-N-(2-{2-[2-({[5-(dimethylamino)naphthalen-1-yl]sulfonyl}amino)ethoxy]ethoxy}ethyl)tricyclo[3.3.1.1~3,7~]decane-1-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 6.5；279 K；12-22% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.1-0.2M KCl, sitting-drop vapor diffusion, temperature 279K
|
Resolution 2.00 Å R-free 0.267 |
| 3P6P Crystal Structure of Cytochrome P450cam crystallized in the presence of a tethered substrate analog AdaC1-C6-Bio Deposited 2010-10-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 A6B (3S,5S,7S)-N-[6-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)hexyl]tricyclo[3.3.1.1~3,7~]decane-1-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 6.5；279 K；12-22% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.1-0.2M KCl, sitting-drop vapor diffusion, temperature 279K
|
Resolution 1.90 Å R-free 0.241 |
| 3P6Q Crystal Structure of Cytochrome P450cam crystallized in the presence of a tethered substrate analog AdaC2-Etg-Boc Deposited 2010-10-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 6.5；279 K；12-22% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.1-0.2M KCl, sitting-drop vapor diffusion, temperature 279K
|
Resolution 1.95 Å R-free 0.238 |
| 3P6R Crystal Structure of Cytochrome P450cam crystallized in the presence of a tethered substrate analog 3OH-AdaC1-Etg-Boc Deposited 2010-10-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 6.5；279 K；12-22% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.1-0.2M KCl, sitting-drop vapor diffusion, temperature 279K
|
Resolution 2.10 Å R-free 0.246 |
| 3P6S Crystal Structure of Cytochrome P450cam crystallized in the presence of a tethered substrate analog AdaC2-C8-Dans Deposited 2010-10-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 6.5；279 K；12-22% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.1-0.2M KCl, sitting-drop vapor diffusion, temperature 279K
|
Resolution 2.00 Å R-free 0.250 |
| 3P6T Crystal Structure of Cytochrome P450cam crystallized in the presence of a tethered substrate analog AdaC2-C8-Dans Deposited 2010-10-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 SBF N-[8-({[5-(dimethylamino)naphthalen-1-yl]sulfonyl}amino)octyl]-2-[(3S,5S,7S)-tricyclo[3.3.1.1~3,7~]dec-1-yl]acetamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 6.5；279 K；12-22% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.1-0.2M KCl, sitting-drop vapor diffusion, temperature 279K
|
Resolution 1.90 Å R-free 0.251 |
| 3P6U Crystal Structure of Cytochrome P450cam crystallized in the presence of a tethered substrate analog AdaC3-C6-Dans Deposited 2010-10-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 6.5；279 K；12-22% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.1-0.2M KCl, sitting-drop vapor diffusion, temperature 279K
|
Resolution 1.70 Å R-free 0.214 |
| 3P6V Crystal Structure of Cytochrome P450cam crystallized in the presence of a tethered substrate analog 3Et-AdaC1-Etg-Boc Deposited 2010-10-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 6.5；279 K；12-22% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.1-0.2M KCl, sitting-drop vapor diffusion, temperature 279K
|
Resolution 2.00 Å R-free 0.242 |
| 3P6W Crystal Structure of Cytochrome P450cam crystallized in the presence of a tethered substrate analog AdaC3-Etg-Boc Deposited 2010-10-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 6.5；279 K；12-22% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.1-0.2M KCl, sitting-drop vapor diffusion, temperature 279K
|
Resolution 2.10 Å R-free 0.263 |
| 3P6X Crystal Structure of Cytochrome P450cam crystallized in the presence of a tethered substrate analog AdaC3-C8-Dans Deposited 2010-10-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 C3Q N-[8-({[5-(dimethylamino)naphthalen-1-yl]sulfonyl}amino)octyl]-3-[(3S,5S,7S)-tricyclo[3.3.1.1~3,7~]dec-1-yl]propanamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 6.5；279 K；12-22% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.1-0.2M KCl, sitting-drop vapor diffusion, temperature 279K
|
Resolution 1.65 Å R-free 0.218 |
| 3W9C Crystal structure of the electron transfer complex of cytochrome p450cam with putidaredoxin Deposited 2013-04-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–415（414 aa）
|
Mutation：K126C, R130C, C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 SO4 SULFATE ION × 4 GOL GLYCEROL × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.1M HEPES (PH7.5), 0.1M SODIUM CHLORIDE, 1.6M AMMONIUM SULFATE, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.50 Å R-free 0.251 |
| 3WRH Crystal structure of P450cam Deposited 2014-02-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.1；279 K；50mM Tris-HCl, 200mM KCl,20-30% PEG 4000, 0.00025mM Camphor, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 1.62 Å R-free 0.209 |
| 3WRH Crystal structure of P450cam Deposited 2014-02-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.1；279 K；50mM Tris-HCl, 200mM KCl,20-30% PEG 4000, 0.00025mM Camphor, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 1.62 Å R-free 0.209 |
| 3WRI Crystal structure of P450cam Deposited 2014-02-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；279 K；50mM Tris-HCl, 200mM KCl,20-30% PEG 4000, 0.001mM Camphor, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 2.90 Å R-free 0.268 |
| 3WRI Crystal structure of P450cam Deposited 2014-02-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；279 K；50mM Tris-HCl, 200mM KCl,20-30% PEG 4000, 0.001mM Camphor, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 2.90 Å R-free 0.268 |
| 3WRJ Crystal structure of P450cam Deposited 2014-02-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.7；279 K；50mM Tris-HCl, 200mM KCl, 20-30% PEG 4000, 0.001mM Camphor, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 1.85 Å R-free 0.221 |
| 3WRJ Crystal structure of P450cam Deposited 2014-02-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.7；279 K；50mM Tris-HCl, 200mM KCl, 20-30% PEG 4000, 0.001mM Camphor, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 1.85 Å R-free 0.221 |
| 3WRK Crystal structure of P450cam Deposited 2014-02-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；279 K；50mM Tris-HCl, 200mM KCl, 20-30% PEG 4000, 0.00025mM Camphor, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 2.61 Å R-free 0.235 |
| 3WRK Crystal structure of P450cam Deposited 2014-02-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；279 K；50mM Tris-HCl, 200mM KCl, 20-30% PEG 4000, 0.00025mM Camphor, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 2.61 Å R-free 0.235 |
| 3WRL Crystal structure of P450cam Deposited 2014-02-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；279 K；50mM Tris-HCl, 200mM KCl, 20-30% PEG 4000, 0.001mM Camphor, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 1.65 Å R-free 0.227 |
| 3WRL Crystal structure of P450cam Deposited 2014-02-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；279 K；50mM Tris-HCl, 200mM KCl, 20-30% PEG 4000, 0.001mM Camphor, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 1.65 Å R-free 0.227 |
| 3WRM Crystal structure of P450cam Deposited 2014-02-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；279 K；50mM Tris-HCl, 200mM KCl, 20-30% PEG 4000, 1mM Camphor, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 1.95 Å R-free 0.233 |
| 3WRM Crystal structure of P450cam Deposited 2014-02-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
1–415（415 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 K POTASSIUM ION × 1 CAM CAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；279 K；50mM Tris-HCl, 200mM KCl, 20-30% PEG 4000, 1mM Camphor, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 279K
|
Resolution 1.95 Å R-free 0.233 |
| 4CP4 CRYSTAL STRUCTURE OF THE CYTOCHROME P450-CAM ACTIVE SITE MUTANT THR252ALA Deposited 1991-06-04 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.10 Å |
| 4CPP CRYSTAL STRUCTURES OF CYTOCHROME P450-CAM COMPLEXED WITH CAMPHANE, THIOCAMPHOR, AND ADAMANTANE: FACTORS CONTROLLING P450 SUBSTRATE HYDROXYLATION Deposited 1990-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ADM ADAMANTANE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.11 Å |
| 4EK1 Crystal Structure of Electron-Spin Labeled Cytochrome P450cam Deposited 2012-04-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：S48C,C58S,C85S,C136S,S190C,C285S,C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 7.4；279 K；12% PEG 8000, 0.1M Tris, pH 7.4, 0.2M KCl, 1mM camphor, sitting-drop vapor diffusion, temperature 279K
|
Resolution 1.97 Å R-free 0.253 |
| 4EK1 Crystal Structure of Electron-Spin Labeled Cytochrome P450cam Deposited 2012-04-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
2–415（414 aa）
|
Mutation：S48C,C58S,C85S,C136S,S190C,C285S,C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting-drop vapor diffusion；pH 7.4；279 K；12% PEG 8000, 0.1M Tris, pH 7.4, 0.2M KCl, 1mM camphor, sitting-drop vapor diffusion, temperature 279K
|
Resolution 1.97 Å R-free 0.253 |
| 4G3R Crystal Structure of Nitrosyl Cytochrome P450cam Deposited 2012-07-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50mM Tris-HCl pH 7.4, 26-32% PEG 4000, 250mM KCl, 1mM D-Camphor, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.20 Å R-free 0.250 |
| 4G3R Crystal Structure of Nitrosyl Cytochrome P450cam Deposited 2012-07-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
2–415（414 aa）
|
Mutation：C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 NO NITRIC OXIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50mM Tris-HCl pH 7.4, 26-32% PEG 4000, 250mM KCl, 1mM D-Camphor, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.20 Å R-free 0.250 |
| 4JWS Crystal structure of Cytochrome P450cam-putidaredoxin complex Deposited 2013-03-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–415（415 aa）
|
Mutation：K344C, C334A, C59S, C86S, C137S, C286S | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CA CALCIUM ION × 1 1N0 1,1'-hexane-1,6-diyldipyrrolidine-2,5-dione × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；0.2 M calcium acetate hydrate, 14-22% PEG3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.15 Å R-free 0.225 |
| 4JWS Crystal structure of Cytochrome P450cam-putidaredoxin complex Deposited 2013-03-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–415（415 aa）
|
Mutation：K344C, C334A, C59S, C86S, C137S, C286S | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CA CALCIUM ION × 1 1N0 1,1'-hexane-1,6-diyldipyrrolidine-2,5-dione × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；0.2 M calcium acetate hydrate, 14-22% PEG3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.15 Å R-free 0.225 |
| 4JWU Crystal structure of Cytochrome P450cam-putidaredoxin complex Deposited 2013-03-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–415（415 aa）
|
Mutation：K344C, C334A, C59S, C86S, C137S, C286S | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CA CALCIUM ION × 1 1N0 1,1'-hexane-1,6-diyldipyrrolidine-2,5-dione × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；0.2 M calcium acetate hydrate, 14-22% PEG3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.20 Å R-free 0.266 |
| 4JWU Crystal structure of Cytochrome P450cam-putidaredoxin complex Deposited 2013-03-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–415（415 aa）
|
Mutation：K344C, C334A, C59S, C86S, C137S, C286S | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CA CALCIUM ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；0.2 M calcium acetate hydrate, 14-22% PEG3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.20 Å R-free 0.266 |
| 4JX1 Crystal structure of reduced Cytochrome P450cam-putidaredoxin complex bound to camphor and 5-exo-hydroxycamphor Deposited 2013-03-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–415（415 aa）
|
Mutation：K344C, C334A, C59S, C86S, C137S, C286S | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 CAH 5-EXO-HYDROXYCAMPHOR × 1 CA CALCIUM ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；0.2 M calcium acetate hydrate, 14-22% PEG3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.09 Å R-free 0.212 |
| 4JX1 Crystal structure of reduced Cytochrome P450cam-putidaredoxin complex bound to camphor and 5-exo-hydroxycamphor Deposited 2013-03-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–415（415 aa）
|
Mutation：K344C, C334A, C59S, C86S, C137S, C286S | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 CA CALCIUM ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；0.2 M calcium acetate hydrate, 14-22% PEG3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.09 Å R-free 0.212 |
| 4JX1 Crystal structure of reduced Cytochrome P450cam-putidaredoxin complex bound to camphor and 5-exo-hydroxycamphor Deposited 2013-03-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–415（415 aa）
|
Mutation：K344C, C334A, C59S, C86S, C137S, C286S | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 CAH 5-EXO-HYDROXYCAMPHOR × 1 CA CALCIUM ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 1N0 1,1'-hexane-1,6-diyldipyrrolidine-2,5-dione × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；0.2 M calcium acetate hydrate, 14-22% PEG3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.09 Å R-free 0.212 |
| 4JX1 Crystal structure of reduced Cytochrome P450cam-putidaredoxin complex bound to camphor and 5-exo-hydroxycamphor Deposited 2013-03-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
1–415（415 aa）
|
Mutation：K344C, C334A, C59S, C86S, C137S, C286S | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 CAH 5-EXO-HYDROXYCAMPHOR × 1 CA CALCIUM ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；0.2 M calcium acetate hydrate, 14-22% PEG3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.09 Å R-free 0.212 |
| 4KKY Crystal structure of N-(1-Pyrene)acetamide labeled P450cam in substrate bound form. Deposited 2013-05-06 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
2–414（413 aa）
|
Mutation：T2A, C58S, C85S, A113C, C136S, C285S, C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 1T4 N-(pyren-1-yl)acetamide × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；287.15 K；20% PEG 8000, 0.2M potassium Chloride, 0.05M Bis-tris Buffer.
1mM camphor., pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 287.15K
|
Resolution 2.00 Å R-free 0.225 |
| 4L49 Structure of L358A mutant of P450cam bound to camphor Deposited 2013-06-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：C334A, L358A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50 mM Tris, 400 mM potassium chloride, 32% PEG4000, 1.2 mM D-camphor, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.13 Å R-free 0.255 |
| 4L4A Structure of L358A/K178G mutant of P450cam bound to camphor Deposited 2013-06-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：C334A, L358A, K178G | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50 mM Tris, 400 mM potassium chloride, 32% PEG4000, 1.2 mM D-camphor, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.10 Å R-free 0.231 |
| 4L4B Structure of L358A/K178G/D182N mutant of P450cam bound to camphor Deposited 2013-06-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：C334A, K178G,L358A,D182N | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50 mM Tris, 400 mM potassium chloride, 32% PEG4000, 1.2 mM D-camphor, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.10 Å R-free 0.235 |
| 4L4C Structure of L358P/K178G mutant of P450cam bound to camphor Deposited 2013-06-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：C334A, L358P, K178G | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50 mM Tris, 400 mM potassium chloride, 32% PEG4000, 1.2 mM D-camphor, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.20 Å R-free 0.243 |
| 4L4C Structure of L358P/K178G mutant of P450cam bound to camphor Deposited 2013-06-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–415（415 aa）
|
Mutation：C334A, L358P, K178G | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50 mM Tris, 400 mM potassium chloride, 32% PEG4000, 1.2 mM D-camphor, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.20 Å R-free 0.243 |
| 4L4D Structure of cyanide and camphor bound P450cam mutant L358A Deposited 2013-06-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：C334A, L358A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CYN CYANIDE ION × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50 mM Tris, 400 mM potassium chloride, 32% PEG4000, 1.2 mM D-camphor, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.10 Å R-free 0.240 |
| 4L4E Structure of cyanide and camphor bound P450cam mutant L358A/K178G Deposited 2013-06-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：C334A, K178G, L358A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CYN CYANIDE ION × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50 mM Tris, 400 mM potassium chloride, 32% PEG4000, 1.2 mM D-camphor, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.26 Å R-free 0.211 |
| 4L4F Structure of cyanide and camphor bound P450cam mutant L358A/K178G/D182N Deposited 2013-06-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：C334A, K178G,L358A,D182N | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CYN CYANIDE ION × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50 mM Tris, 400 mM potassium chloride, 32% PEG4000, 1.2 mM D-camphor, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.29 Å R-free 0.215 |
| 4L4G Structure of cyanide and camphor bound P450cam mutant L358P/K178G Deposited 2013-06-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：C334A, K178G, L358P | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CYN CYANIDE ION × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50 mM Tris, 400 mM potassium chloride, 32% PEG4000, 1.2 mM D-camphor, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.55 Å R-free 0.212 |
| 5CP4 CRYOGENIC STRUCTURE OF P450CAM Deposited 1998-05-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；pH 7.0
|
Resolution 1.75 Å R-free 0.260 |
| 5CPP THE STRUCTURAL BASIS FOR SUBSTRATE-INDUCED CHANGES IN REDOX POTENTIAL AND SPIN EQUILIBRIUM IN CYTOCHROME P-450(CAM) Deposited 1990-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ADO ADAMANTANONE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.08 Å |
| 5GXG High-resolution crystal structure of the electron transfer complex of cytochrome p450cam with putidaredoxin Deposited 2016-09-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–415（414 aa）
|
Mutation：K126C,R130C, C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 SO4 SULFATE ION × 5 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；289 K；0.1M HEPES, 0.1M Sodium Chloride, 1.6M Ammonium sulfate
|
Resolution 1.70 Å R-free 0.192 |
| 5GXG High-resolution crystal structure of the electron transfer complex of cytochrome p450cam with putidaredoxin Deposited 2016-09-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
2–415（414 aa）
|
Mutation：K126C,R130C, C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 2 SO4 SULFATE ION × 10 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；289 K；0.1M HEPES, 0.1M Sodium Chloride, 1.6M Ammonium sulfate
|
Resolution 1.70 Å R-free 0.192 |
| 5IK1 Open state of P450cam after soaking in camphor Deposited 2016-03-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
10–415（406 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；100 mM Bis-Tris
10-22 % PEG 8000
200 mM KCl
|
Resolution 1.53 Å R-free 0.205 |
| 5WK7 P450cam mutant R186A Deposited 2017-07-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：R186A, C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAH 5-EXO-HYDROXYCAMPHOR × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50mM Tris, pH 7.4, 400mM KCl, 32% PEG4000, 1.2 mM D-camphor
|
Resolution 1.98 Å R-free 0.215 |
| 5WK9 R186AP450cam with CN and camphor Deposited 2017-07-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：R186A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CYN CYANIDE ION × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50 mM potassium phosphate pH 7.4, 400 mM KCl, 32%PEG4000
|
Resolution 1.98 Å R-free 0.215 |
| 6CP4 P450CAM D251N MUTANT Deposited 1998-05-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Mutation：D251N | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；pH 7.0
|
Resolution 1.90 Å R-free 0.270 |
| 6CPP CRYSTAL STRUCTURES OF CYTOCHROME P450-CAM COMPLEXED WITH CAMPHANE, THIOCAMPHOR, AND ADAMANTANE: FACTORS CONTROLLING P450 SUBSTRATE HYDROXYLATION Deposited 1990-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAE CAMPHANE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 6NBL Cytochrome P450cam-putidaredoxin complex bound to camphor and cyanide Deposited 2018-12-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–415（415 aa）
|
Mutation：C334A, K344C, C59S, C86S, C137S, C268S | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CYN CYANIDE ION × 1 CAM CAMPHOR × 1 CA CALCIUM ION × 1 1N0 1,1'-hexane-1,6-diyldipyrrolidine-2,5-dione × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M calcium acetate hydrate, 14-22% PEG3350, pH 7.4, 50 mM KCN
|
Resolution 2.15 Å R-free 0.254 |
| 6NBL Cytochrome P450cam-putidaredoxin complex bound to camphor and cyanide Deposited 2018-12-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–415（415 aa）
|
Mutation：C334A, K344C, C59S, C86S, C137S, C268S | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CYN CYANIDE ION × 1 CAM CAMPHOR × 1 CA CALCIUM ION × 1 1N0 1,1'-hexane-1,6-diyldipyrrolidine-2,5-dione × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M calcium acetate hydrate, 14-22% PEG3350, pH 7.4, 50 mM KCN
|
Resolution 2.15 Å R-free 0.254 |
| 6WE6 Camphor bound P450cam D251E structure Deposited 2020-04-01 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：D251E/C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50 mM Tris, 10% PEG 4000, 200 mM potassium chloride, 2 mM D-camphor
|
Resolution 2.16 Å R-free 0.256 |
| 6WE6 Camphor bound P450cam D251E structure Deposited 2020-04-01 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–415（415 aa）
|
Mutation：D251E/C334A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAM CAMPHOR × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；50 mM Tris, 10% PEG 4000, 200 mM potassium chloride, 2 mM D-camphor
|
Resolution 2.16 Å R-free 0.256 |
| 6WFL Camphor soaked P450cam D251E Deposited 2020-04-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：D251E | K POTASSIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CAH 5-EXO-HYDROXYCAMPHOR × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；100 mM Bis-Tris pH 6.5, 20% PEG 8000, 200 mM potassium chloride, 5 mM DTT
|
Resolution 1.60 Å R-free 0.254 |
| 7CPP THE STRUCTURAL BASIS FOR SUBSTRATE-INDUCED CHANGES IN REDOX POTENTIAL AND SPIN EQUILIBRIUM IN CYTOCHROME P450(CAM) Deposited 1990-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NCM NORCAMPHOR × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 8CPP CRYSTAL STRUCTURES OF CYTOCHROME P450-CAM COMPLEXED WITH CAMPHANE, THIOCAMPHOR, AND ADAMANTANE: FACTORS CONTROLLING P450 SUBSTRATE HYDROXYLATION Deposited 1990-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–414（414 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 TCM THIOCAMPHOR × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.10 Å |
| 9L7R Crystal structure of P450cam-F87R mutant complex with (-)-ambroxide Deposited 2024-12-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–415（415 aa）
|
Mutation：F88R | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 A1EI5 (3~{a}~{R},5~{a}~{S},9~{a}~{S},9~{b}~{R})-3~{a},6,6,9~{a}-tetramethyl-2,4,5,5~{a},7,8,9,9~{b}-octahydro-1~{H}-benzo[e][1]benzofuran × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.04 M citric acid,0.06 M bis-tris, pH6.4, 24% PEG3350
|
Resolution 2.04 Å R-free 0.255 |
| 9L7R Crystal structure of P450cam-F87R mutant complex with (-)-ambroxide Deposited 2024-12-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–415（415 aa）
|
Mutation：F88R | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 A1EI5 (3~{a}~{R},5~{a}~{S},9~{a}~{S},9~{b}~{R})-3~{a},6,6,9~{a}-tetramethyl-2,4,5,5~{a},7,8,9,9~{b}-octahydro-1~{H}-benzo[e][1]benzofuran × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.04 M citric acid,0.06 M bis-tris, pH6.4, 24% PEG3350
|
Resolution 2.04 Å R-free 0.255 |
128 other PDB entries and 171 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | CPXA_PSEPU |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–414； UniProt 1–414 Author chain B； PDBConstruct 1–414； UniProt 1–414 |
