SAXSdb — 1aro

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1. Structure Basics

entry_id	1aro
deposition_date	1997-08-08
title	T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME
keywords	;TRANSCRIPTION, DNA-DIRECTED RNA POLYMERASE, HYDROLASE, GLYCOSIDASE, COMPLEX (POLYMERASE-HYDROLASE), COMPLEX (POLYMERASE-HYDROLASE) complex ;; COMPLEX (POLYMERASE/HYDROLASE)
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	32.13 Å
rg_electron	31.48 Å
i0	180262000.00
molecular_weight	105100.0 kDa
excluded_volume	130420 Å³
envelope_volume	172340 Å³
shell_volume	45145 Å³
envelope_diameter	107.7 Å
shell_rg	38.98 Å
envelope_rg	31.19 Å
shape_rg	31.51 Å
total_rg	32.01 Å
total_atoms	7314
n_residues	923
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	98.1 Å
rg_real	31.97 Å
rg_real_error	0.54 Å
i0_real	1.8030e+08
i0_real_error	2.6700e+06
rg_reciprocal	32.04 Å
i0_reciprocal	180300000.0000
total_estimate	0.9059
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	42.0 Å
skewness	0.180
kurtosis	-0.469
angular_range	— – 0.2450 Å⁻¹
current_alpha	0.0000
highest_alpha	34630000.0000
n_real_points	50
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.956; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.993; Smooth: 0.909

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	T7 RNA POLYMERASE
formula_weight	98936.0 kDa
ec_number	2.7.7.6
mutation	C347S, C723S, C839S
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	P
atom_count	7314

MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVITKWKHSPVEDIPAIEREELPMKPEDIDMNPEALTAWKRAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAKKVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILRKRSAVHWVTPDGFPVWQEYKKPIQTRLNLMFLGQFRLQPTINTNKDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADAANLFKAVRETMVDTYESSDVLADFYDQFADQLHESQLDKMPALPAKGNLNLRDILESDFAFA

Source

organism	Enterobacteria phage T7
ncbi_taxonomy_id	10760
host_organism	Escherichia coli BL21

Entity 2 polymer

description	T7 LYSOZYME
formula_weight	17007.3 kDa
ec_number	3.5.1.28
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	L

MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDVGYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSLRSLLVTLLAKYEGAVLRAHHEVAPKACPSFDLKRWWEKNELVTSDRG

Source

organism	Enterobacteria phage T7
ncbi_taxonomy_id	10760
host_organism	Escherichia coli BL21

Entity 3 non-polymer

description	MERCURY (II) ION
formula_weight	200.6 kDa
src_method	syn
entity_count	7

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6. Citations (1)

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length_a	273.385 Å
length_b	95.612 Å
length_c	63.582 Å
angle_alpha	90.00 °
angle_beta	101.40 °
angle_gamma	90.00 °
z_pdb	4

space_group	C 1 2 1
int_tables_number	5
crystal_system	—

resolution	2.800 Å
resolution_low	30.000 Å
r_factor	0.2620
r_work	0.2620
r_free	0.3090

fit	/app/data/saxs_computation/ar/1aro/1aro.fit
pddf	/app/data/pr_computation/ar/1aro/1aro.pr.out
profile	/app/data/saxs_computation/ar/1aro/1aro.dat

T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)