SAXSdb — 1cez

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1. Structure Basics

entry_id	1cez
deposition_date	1999-03-11
title	CRYSTAL STRUCTURE OF A T7 RNA POLYMERASE-T7 PROMOTER COMPLEX
keywords	T7 RNA POLYMERASE, TRANSFERASE-DNA COMPLEX; TRANSFERASE/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	31.27 Å
rg_electron	29.91 Å
i0	192462000.00
molecular_weight	104140.0 kDa
excluded_volume	127650 Å³
envelope_volume	166980 Å³
shell_volume	45231 Å³
envelope_diameter	105.8 Å
shell_rg	38.30 Å
envelope_rg	29.78 Å
shape_rg	29.88 Å
total_rg	30.69 Å
total_atoms	7295
n_residues	894
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	99.4 Å
rg_real	31.09 Å
rg_real_error	0.68 Å
i0_real	1.9250e+08
i0_real_error	2.9260e+06
rg_reciprocal	31.17 Å
i0_reciprocal	192500000.0000
total_estimate	0.8955
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	40.9 Å
skewness	0.148
kurtosis	-0.413
angular_range	— – 0.2550 Å⁻¹
current_alpha	0.0000
highest_alpha	26320000.0000
n_real_points	52
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.885; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.995; Smooth: 0.987

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	;DNA (5'-D(PTPAPTPAPGPTPGPAPGPTPCPGPTPAPTPTP*A)-3') ;
formula_weight	5256.4 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	T
atom_count	7766

TATAGTGAGTCGTATTA

Entity 2 polymer

description	;DNA (5'-D(PTPAPAPTPAPCPGPAPCPTPCPAPCPTP*A)-3') ;
formula_weight	4537.0 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	N

TAATACGACTCACTA

Entity 3 polymer

description	PROTEIN (BACTERIOPHAGE T7 RNA POLYMERASE)
formula_weight	98984.2 kDa
ec_number	2.7.7.6
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A

MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVEDIPAIEREELPMKPEDIDMNPEALTAWKRAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAKKVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLGQFRLQPTINTNKDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLNLRDILESDFAFA

Source

organism	Enterobacteria phage T7
ncbi_taxonomy_id	10760
host_organism	Escherichia coli BL21

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	471

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6. Citations (1)

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length_a	220.100 Å
length_b	73.300 Å
length_c	80.900 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 2
int_tables_number	18
crystal_system	—

resolution	2.400 Å
resolution_low	40.000 Å
r_factor	0.2240
r_work	0.2240
r_free	0.2700

fit	/app/data/saxs_computation/ce/1cez/1cez.fit
pddf	/app/data/pr_computation/ce/1cez/1cez.pr.out
profile	/app/data/saxs_computation/ce/1cez/1cez.dat

CRYSTAL STRUCTURE OF A T7 RNA POLYMERASE-T7 PROMOTER COMPLEX

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

Entity 4 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)