SAXSdb — 1atn

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1. Structure Basics

entry_id	1atn
deposition_date	1991-03-08
title	Atomic structure of the actin:DNASE I complex
keywords	ENDODEOXYRIBONUCLEASE; ENDODEOXYRIBONUCLEASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	30.78 Å
rg_electron	30.33 Å
i0	83303400.00
molecular_weight	71563.0 kDa
excluded_volume	89175 Å³
envelope_volume	107940 Å³
shell_volume	30328 Å³
envelope_diameter	102.6 Å
shell_rg	36.62 Å
envelope_rg	30.03 Å
shape_rg	30.32 Å
total_rg	30.92 Å
total_atoms	5019
n_residues	629
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	96.9 Å
rg_real	30.86 Å
rg_real_error	0.77 Å
i0_real	8.3300e+07
i0_real_error	1.2640e+06
rg_reciprocal	30.83 Å
i0_reciprocal	83300000.0000
total_estimate	0.8869
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	28.5 Å
skewness	0.328
kurtosis	-0.624
angular_range	— – 0.2550 Å⁻¹
current_alpha	0.0000
highest_alpha	15590000.0000
n_real_points	52
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.933; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.916; Smooth: 0.811

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	ACTIN
formula_weight	41522.2 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	5019

XDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHR

Entity 2 polymer

description	DEOXYRIBONUCLEASE I
formula_weight	29047.5 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	D

LKIAAFNIRTFGETKMSNATLASYIVRIVRRYDIVLIQEVRDSHLVAVGKLLDYLNQDDPNTYHYVVSEPLGRNSYKERYLFLFRPNKVSVLDTYQYDDGCGNCGNDSFSREPAVVKFSSHSTKVKEFAIVALHSAPSDAVAEINSLYDVYLDVQQKWHLNDVMLMGDFNADCSYVTSSQWSSIRLRTSSTFQWLIPDSADTTATSTNCAYDRIVVAGSLLQSSVVPGSAAPFDFQAAYGLSNEMALAISDHYPVEVTLT

Entity 3 branched

description	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	586.5 kDa
src_method	man
entity_count	1

Entity 4 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	4

Entity 5 non-polymer

description	ADENOSINE-5'-TRIPHOSPHATE
formula_weight	507.2 kDa
src_method	syn
entity_count	1

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6. Citations (9)

Atomic structure of the actin:DNase I complex.

Nature (1990)

PMID: 2395459 · DOI: 10.1038/347037a0 PRIMARY

Structure and Function of Actin

Annu.Rev.Biophys.Biophys.Chem. (1992)

Muscle Proteins: Actin

Curr.Opin.Struct.Biol. (1991)

Similarity of the Three-Dimensional Structures of Actin and the ATPase Fragment of a 70-kDa Heat Shock Cognate Protein

Proc.Natl.Acad.Sci.USA (1991)

Atomic Model of the Actin Filament

Nature (1990)

Crystallographic Refinement and Structure of DNase I at 2 Angstroms Resolution

J.Mol.Biol. (1986)

Three-Dimensional Structure of the Complex of Actin and DNase I at 4.5 Angstroms Resolution

Embo J. (1985)

Three-Dimensional Structure of Bovine Pancreatic DNase I at 2.5 Angstroms Resolution

Embo J. (1984)

Three-Dimensional Structure of the Complex of Skeletal Muscle Actin and Bovine Pancreatic DNase I at 6-Angstroms Resolution

Proc.Natl.Acad.Sci.USA (1981)

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length_a	132.900 Å
length_b	56.300 Å
length_c	109.700 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	2.800 Å
resolution_low	10.000 Å
r_factor	—
r_work	0.2100
r_free	—

fit	/app/data/saxs_computation/at/1atn/1atn.fit
pddf	/app/data/pr_computation/at/1atn/1atn.pr.out
profile	/app/data/saxs_computation/at/1atn/1atn.dat

Atomic structure of the actin:DNASE I complex

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 branched

Entity 4 non-polymer

Entity 5 non-polymer

6. Citations (9)

7. Files & Curves (10)

Files (7)

Curves (3)