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1axm

HEPARIN-LINKED BIOLOGICALLY-ACTIVE DIMER OF FIBROBLAST GROWTH FACTOR

Method: X-RAY DIFFRACTION D_max: 197.2 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1axm
deposition_date	1997-10-16
title	HEPARIN-LINKED BIOLOGICALLY-ACTIVE DIMER OF FIBROBLAST GROWTH FACTOR
keywords	HUMAN ACIDIC FIBROBLAST GROWTH FACTOR, HEPARIN DECASACCHARIDE, GROWTH FACTOR; GROWTH FACTOR
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	62.04 Å
rg_electron	63.46 Å
i0	133342000.00
molecular_weight	91613.0 kDa
excluded_volume	112590 Å³
envelope_volume	191680 Å³
shell_volume	27807 Å³
envelope_diameter	206.3 Å
shell_rg	56.86 Å
envelope_rg	60.64 Å
shape_rg	63.48 Å
total_rg	63.20 Å
total_atoms	6392
n_residues	764
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	197.2 Å
rg_real	63.10 Å
rg_real_error	2.70 Å
i0_real	1.3330e+08
i0_real_error	2.9760e+06
rg_reciprocal	61.07 Å
i0_reciprocal	132900000.0000
total_estimate	0.6547
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	23.7 Å
skewness	0.371
kurtosis	-0.653
angular_range	— – 0.1250 Å⁻¹
current_alpha	0.0000
highest_alpha	2626000.0000
n_real_points	26
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.256; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.397; Smooth: 0.343

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	ACIDIC FIBROBLAST GROWTH FACTOR
formula_weight	15336.1 kDa
src_method	man
entity_count	6

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E,F
atom_count	6463

GNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 2 branched

description	;2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose ;
formula_weight	1494.2 kDa
src_method	man
entity_count	2

Entity 3 branched

description	;2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid ;
formula_weight	1429.1 kDa
src_method	man
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	71

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6. Citations (4)

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length_a	87.690 Å
length_b	158.050 Å
length_c	190.580 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	48

space_group	C 2 2 21
int_tables_number	20
crystal_system	—

resolution	3.000 Å
resolution_low	14.000 Å
r_factor	0.2170
r_work	0.2170
r_free	0.3040

fit	/app/data/saxs_computation/ax/1axm/1axm.fit
pddf	/app/data/pr_computation/ax/1axm/1axm.pr.out
profile	/app/data/saxs_computation/ax/1axm/1axm.dat

HEPARIN-LINKED BIOLOGICALLY-ACTIVE DIMER OF FIBROBLAST GROWTH FACTOR

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction