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1b23

E. coli cysteinyl-tRNA and T. aquaticus elongation factor EF-TU:GTP ternary complex

Method: X-RAY DIFFRACTION D_max: 111.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1b23
deposition_date	1998-12-04
title	E. coli cysteinyl-tRNA and T. aquaticus elongation factor EF-TU:GTP ternary complex
keywords	TRANSLATION ELONGATION FACTOR, TRANSFER RNA, PROTEIN SYNTHESIS, GENE REGULATION-RNA COMPLEX; GENE REGULATION/RNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	30.58 Å
rg_electron	29.76 Å
i0	122777000.00
molecular_weight	69625.0 kDa
excluded_volume	79285 Å³
envelope_volume	104640 Å³
shell_volume	31711 Å³
envelope_diameter	116.8 Å
shell_rg	34.14 Å
envelope_rg	30.04 Å
shape_rg	29.67 Å
total_rg	30.29 Å
total_atoms	4779
n_residues	471
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	111.6 Å
rg_real	30.91 Å
rg_real_error	1.17 Å
i0_real	1.2280e+08
i0_real_error	1.8880e+06
rg_reciprocal	30.77 Å
i0_reciprocal	122800000.0000
total_estimate	0.8107
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	32.2 Å
skewness	0.658
kurtosis	0.132
angular_range	— – 0.2600 Å⁻¹
current_alpha	0.0000
highest_alpha	9803000.0000
n_real_points	53
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.650; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.690; Smooth: 0.895

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4. Crystallography & Experiment

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5. Entities & Polymer Info (7)

Entity 1 polymer

description	CYSTEINYL TRNA
formula_weight	23927.5 kDa
src_method	man
entity_count	1

Polymer

type	polyribonucleotide
strand_id	R
atom_count	5040

GGCGCGUUAACAAAGCGGUUAUGUAGCGGAUUGCAAAUCCGUCUAGUCCGGUUCGACUCCGGAACGCGCCUCCA

Source

organism	Escherichia coli
ncbi_taxonomy_id	562

Entity 2 polymer

description	ELONGATION FACTOR TU
formula_weight	44743.0 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	P

AKGEFIRTKPHVNVGTIGHVDHGKTTLTAALTYVAAAENPNVEVKDYGDIDKAPEERARGITINTAHVEYETAKRHYSHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFMNKVDMVDDPELLDLVEMEVRDLLNQYEFPGDEVPVIRGSALLALEEMHKNPKTKRGENEWVDKIWELLDAIDEYIPTPVRDVDKPFLMPVEDVFTITGRGTVATGRIERGKVKVGDEVEIVGLAPETRKTVVTGVEMHRKTLQEGIAGDNVGLLLRGVSREEVERGQVLAKPGSITPHTKFEASVYILKKEEGGRHTGFFTGYRPQFYFRTTDVTGVVRLPQGVEMVMPGDNVTFTVELIKPVALEEGLRFAIREGGRTVGAGVVTKILE

Source

organism	Thermus aquaticus
ncbi_taxonomy_id	271
host_organism	Escherichia coli

Entity 3 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	3

Entity 4 non-polymer

description	CYSTEINE
formula_weight	121.2 kDa
src_method	syn
entity_count	1

Entity 5 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	2

Entity 6 non-polymer

description	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
formula_weight	522.2 kDa
src_method	syn
entity_count	1

Entity 7 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	261

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6. Citations (2)

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length_a	126.750 Å
length_b	132.980 Å
length_c	154.880 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	F 2 2 2
int_tables_number	22
crystal_system	—

resolution	2.600 Å
resolution_low	10.000 Å
r_factor	0.2060
r_work	0.2060
r_free	0.2630

fit	/app/data/saxs_computation/b2/1b23/1b23.fit
pddf	/app/data/pr_computation/b2/1b23/1b23.pr.out
profile	/app/data/saxs_computation/b2/1b23/1b23.dat

E. coli cysteinyl-tRNA and T. aquaticus elongation factor EF-TU:GTP ternary complex

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (7)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 non-polymer

Entity 6 non-polymer

Entity 7 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)