← Back to search

1bk5

KARYOPHERIN ALPHA FROM SACCHAROMYCES CEREVISIAE

Method: X-RAY DIFFRACTION D_max: 127.7 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1bk5
deposition_date	1998-07-14
title	KARYOPHERIN ALPHA FROM SACCHAROMYCES CEREVISIAE
keywords	PROTEIN TRANSPORT, NLS NUCLEAR IMPORT, ARMADILLO REPEAT CONTAINING PROTEIN; PROTEIN TRANSPORT
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	35.09 Å
rg_electron	34.83 Å
i0	132047000.00
molecular_weight	93413.0 kDa
excluded_volume	117590 Å³
envelope_volume	149190 Å³
shell_volume	37265 Å³
envelope_diameter	135.5 Å
shell_rg	39.31 Å
envelope_rg	34.70 Å
shape_rg	34.82 Å
total_rg	35.19 Å
total_atoms	6552
n_residues	844
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	127.7 Å
rg_real	35.36 Å
rg_real_error	1.21 Å
i0_real	1.3200e+08
i0_real_error	2.3220e+06
rg_reciprocal	35.19 Å
i0_reciprocal	132000000.0000
total_estimate	0.6169
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	39.7 Å
skewness	0.561
kurtosis	-0.016
angular_range	— – 0.2250 Å⁻¹
current_alpha	0.0000
highest_alpha	23100000.0000
n_real_points	46
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.711; Stabil: 1.000; Sysdev: 0.062; Positv: 1.000; Valcen: 0.792; Smooth: 0.903

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (3)

Entity 1 polymer

description	KARYOPHERIN ALPHA
formula_weight	46718.4 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	6782

LPQMTQQLNSDDMQEQLSATVKFRQILSREHRPPIDVVIQAGVVPRLVEFMRENQPEMLQLEAAWALTNIASGTSAQTKVVVDADAVPLFIQLLYTGSVEVKEQAIWALGNVAGDSTDYRDYVLQCNAMEPILGLFNSNKPSLIRTATWTLSNLCRGKKPQPDWSVVSQALPTLAKLIYSMDTETLVDACWAISYLSDGPQEAIQAVIDVRIPKRLVELLSHESTLVQTPALRAVGNIVTGNDLQTQVVINAGVLPALRLLLSSPKENIKKEACWTISNITAGNTEQIQAVIDANLIPPLVKLLEVAEYKTKKEACWAISNASSGGLQRPDIIRYLVSQGCIKPLCDLLEIADNRIIEVTLDALENILKMGEADKEARGLNINENADFIEKAGGMEKIFNCQQNENDKIYEKAYKIIETYFG

Source

organism	Saccharomyces cerevisiae
common_name	;baker's yeast ;
ncbi_taxonomy_id	4932
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	COBALT (II) ION
formula_weight	58.9 kDa
src_method	syn
entity_count	2

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	230

▶

6. Citations (1)

▶

length_a	157.980 Å
length_b	74.140 Å
length_c	84.050 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	2.200 Å
resolution_low	19.500 Å
r_factor	0.2370
r_work	0.2370
r_free	0.2760

fit	/app/data/saxs_computation/bk/1bk5/1bk5.fit
pddf	/app/data/pr_computation/bk/1bk5/1bk5.pr.out
profile	/app/data/saxs_computation/bk/1bk5/1bk5.dat

KARYOPHERIN ALPHA FROM SACCHAROMYCES CEREVISIAE

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction