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1ee4

CRYSTAL STRUCTURE OF YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A C-MYC NLS PEPTIDE

Method: X-RAY DIFFRACTION D_max: 186.0 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ee4
deposition_date	2000-01-30
title	CRYSTAL STRUCTURE OF YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A C-MYC NLS PEPTIDE
keywords	ARM repeat, TRANSPORT PROTEIN; TRANSPORT PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	57.39 Å
rg_electron	58.46 Å
i0	118043000.00
molecular_weight	92662.0 kDa
excluded_volume	117040 Å³
envelope_volume	176300 Å³
shell_volume	27799 Å³
envelope_diameter	200.1 Å
shell_rg	52.78 Å
envelope_rg	56.07 Å
shape_rg	58.46 Å
total_rg	58.28 Å
total_atoms	6691
n_residues	873
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	186.0 Å
rg_real	58.46 Å
rg_real_error	2.24 Å
i0_real	1.1800e+08
i0_real_error	2.4210e+06
rg_reciprocal	56.42 Å
i0_reciprocal	117700000.0000
total_estimate	0.5859
solution_quality	REASONABLE a REASONABLE solution
n_peaks	4
r_peak_primary	27.6 Å
skewness	0.446
kurtosis	-0.827
angular_range	— – 0.1350 Å⁻¹
current_alpha	0.0000
highest_alpha	2233000.0000
n_real_points	28
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.179; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.065; Smooth: 0.010

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	KARYOPHERIN ALPHA
formula_weight	46870.5 kDa
mutation	Y397D
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	6991

QELPQMTQQLNSDDMQEQLSATVKFRQILSREHRPPIDVVIQAGVVPRLVEFMRENQPEMLQLEAAWALTNIASGTSAQTKVVVDADAVPLFIQLLYTGSVEVKEQAIWALGNVAGDSTDYRDYVLQCNAMEPILGLFNSNKPSLIRTATWTLSNLCRGKKPQPDWSVVSQALPTLAKLIYSMDTETLVDACWAISYLSDGPQEAIQAVIDVRIPKRLVELLSHESTLVQTPALRAVGNIVTGNDLQTQVVINAGVLPALRLLLSSPKENIKKEACWTISNITAGNTEQIQAVIDANLIPPLVKLLEVAEDKTKKEACWAISNASSGGLQRPDIIRYLVSQGCIKPLCDLLEIADNRIIEVTLDALENILKMGEADKEARGLNINENADFIEKAGGMEKIFNCQQNENDKIYEKAYKIIETYF

Source

organism	Saccharomyces cerevisiae
common_name	;baker's yeast ;
ncbi_taxonomy_id	4932

Entity 2 polymer

description	MYC PROTO-ONCOGENE PROTEIN
formula_weight	1000.2 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	C,D,E,F

PAAKRVKLD

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	300

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6. Citations (1)

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length_a	42.680 Å
length_b	85.850 Å
length_c	117.500 Å
angle_alpha	90.00 °
angle_beta	93.42 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	2.100 Å
resolution_low	30.000 Å
r_factor	—
r_work	0.2460
r_free	0.2770

fit	/app/data/saxs_computation/ee/1ee4/1ee4.fit
pddf	/app/data/pr_computation/ee/1ee4/1ee4.pr.out
profile	/app/data/saxs_computation/ee/1ee4/1ee4.dat

CRYSTAL STRUCTURE OF YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A C-MYC NLS PEPTIDE

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)