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1ee5

YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A NUCLEOPLASMIN NLS PEPTIDE

Method: X-RAY DIFFRACTION D_max: 101.8 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ee5
deposition_date	2000-01-30
title	YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A NUCLEOPLASMIN NLS PEPTIDE
keywords	Armadillo repeat, TRANSPORT PROTEIN; TRANSPORT PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	27.98 Å
rg_electron	27.85 Å
i0	33474100.00
molecular_weight	45867.0 kDa
excluded_volume	57870 Å³
envelope_volume	70470 Å³
shell_volume	23153 Å³
envelope_diameter	105.7 Å
shell_rg	32.20 Å
envelope_rg	28.13 Å
shape_rg	27.87 Å
total_rg	28.24 Å
total_atoms	3310
n_residues	440
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	101.8 Å
rg_real	28.41 Å
rg_real_error	1.18 Å
i0_real	3.3470e+07
i0_real_error	5.9260e+05
rg_reciprocal	28.28 Å
i0_reciprocal	33470000.0000
total_estimate	0.5538
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	23.2 Å
skewness	0.612
kurtosis	-0.239
angular_range	— – 0.2850 Å⁻¹
current_alpha	0.0000
highest_alpha	13790000.0000
n_real_points	58
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.522; Stabil: 0.999; Sysdev: 0.075; Positv: 1.000; Valcen: 0.425; Smooth: 0.982

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	KARYOPHERIN ALPHA
formula_weight	46927.6 kDa
mutation	Y397D
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	3342

QELPQMTQQLNSDDMQEQLSATVKFRQILSREHRPPIDVVIQAGVVPRLVEFMRENQPEMLQLEAAWALTNIASGTSAQTKVVVDADAVPLFIQLLYTGSVEVKEQAIWALGNVAGDSTDYRDYVLQCNAMEPILGLFNSNKPSLIRTATWTLSNLCRGKKPQPDWSVVSQALPTLAKLIYSMDTETLVDACWAISYLSDGPQEAIQAVIDVRIPKRLVELLSHESTLVQTPALRAVGNIVTGNDLQTQVVINAGVLPALRLLLSSPKENIKKEACWTISNITAGNTEQIQAVIDANLIPPLVKLLEVAEDKTKKEACWAISNASSGGLQRPDIIRYLVSQGCIKPLCDLLEIADNRIIEVTLDALENILKMGEADKEARGLNINENADFIEKAGGMEKIFNCQQNENDKIYEKAYKIIETYFG

Source

organism	Saccharomyces cerevisiae
common_name	;baker's yeast ;
ncbi_taxonomy_id	4932

Entity 2 polymer

description	NUCLEOPLASMIN
formula_weight	2030.5 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

AVKRPAATKKAGQAKKKKL

Source

organism	Xenopus laevis
common_name	African clawed frog
ncbi_taxonomy_id	8355

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	32

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6. Citations (1)

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length_a	157.560 Å
length_b	63.700 Å
length_c	62.150 Å
angle_alpha	90.00 °
angle_beta	97.34 °
angle_gamma	90.00 °
z_pdb	4

space_group	C 1 2 1
int_tables_number	5
crystal_system	—

resolution	2.400 Å
resolution_low	30.000 Å
r_factor	—
r_work	0.2410
r_free	0.2650

fit	/app/data/saxs_computation/ee/1ee5/1ee5.fit
pddf	/app/data/pr_computation/ee/1ee5/1ee5.pr.out
profile	/app/data/saxs_computation/ee/1ee5/1ee5.dat

YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A NUCLEOPLASMIN NLS PEPTIDE

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)