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1bk6

KARYOPHERIN ALPHA (YEAST) + SV40 T ANTIGEN NLS

Method: X-RAY DIFFRACTION D_max: 128.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1bk6
deposition_date	1998-07-14
title	KARYOPHERIN ALPHA (YEAST) + SV40 T ANTIGEN NLS
keywords	;PROTEIN TRANSPORT, NLS NUCLEAR IMPORT, ARMADILLO REPEAT CONTAINING PROTEIN, COMPLEX (PROTEIN TRANSPORT-PEPTIDE), COMPLEX (PROTEIN TRANSPORT-PEPTIDE) complex ;; COMPLEX (PROTEIN TRANSPORT/PEPTIDE)
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	35.11 Å
rg_electron	34.95 Å
i0	126319000.00
molecular_weight	92303.0 kDa
excluded_volume	116580 Å³
envelope_volume	148420 Å³
shell_volume	36905 Å³
envelope_diameter	135.8 Å
shell_rg	39.48 Å
envelope_rg	34.82 Å
shape_rg	34.94 Å
total_rg	35.32 Å
total_atoms	6488
n_residues	866
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	128.1 Å
rg_real	35.36 Å
rg_real_error	1.89 Å
i0_real	1.2630e+08
i0_real_error	2.3980e+06
rg_reciprocal	35.20 Å
i0_reciprocal	126300000.0000
total_estimate	0.8252
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	37.0 Å
skewness	0.550
kurtosis	-0.039
angular_range	— – 0.2250 Å⁻¹
current_alpha	0.0000
highest_alpha	30270000.0000
n_real_points	46
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.690; Stabil: 0.993; Sysdev: 1.000; Positv: 1.000; Valcen: 0.787; Smooth: 0.885

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	KARYOPHERIN ALPHA
formula_weight	46718.4 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	6500

LPQMTQQLNSDDMQEQLSATVKFRQILSREHRPPIDVVIQAGVVPRLVEFMRENQPEMLQLEAAWALTNIASGTSAQTKVVVDADAVPLFIQLLYTGSVEVKEQAIWALGNVAGDSTDYRDYVLQCNAMEPILGLFNSNKPSLIRTATWTLSNLCRGKKPQPDWSVVSQALPTLAKLIYSMDTETLVDACWAISYLSDGPQEAIQAVIDVRIPKRLVELLSHESTLVQTPALRAVGNIVTGNDLQTQVVINAGVLPALRLLLSSPKENIKKEACWTISNITAGNTEQIQAVIDANLIPPLVKLLEVAEYKTKKEACWAISNASSGGLQRPDIIRYLVSQGCIKPLCDLLEIADNRIIEVTLDALENIIKMGEADKEARGLNINENADFIEKAGGMEKIFNCQQNENDKIYEKAYKIIETYFG

Source

organism	Saccharomyces cerevisiae
common_name	;baker's yeast ;
ncbi_taxonomy_id	4932
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	LARGE T ANTIGEN
formula_weight	791.1 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	C,E

KKKRKV

Entity 3 polymer

description	LARGE T ANTIGEN
formula_weight	489.6 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	D,F

AKKAA

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	12

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6. Citations (1)

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length_a	161.120 Å
length_b	75.750 Å
length_c	84.460 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	2.800 Å
resolution_low	29.100 Å
r_factor	0.2560
r_work	0.2560
r_free	0.3070

fit	/app/data/saxs_computation/bk/1bk6/1bk6.fit
pddf	/app/data/pr_computation/bk/1bk6/1bk6.pr.out
profile	/app/data/saxs_computation/bk/1bk6/1bk6.dat

KARYOPHERIN ALPHA (YEAST) + SV40 T ANTIGEN NLS

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Entity 4 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)