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1cvj

X-RAY CRYSTAL STRUCTURE OF THE POLY(A)-BINDING PROTEIN IN COMPLEX WITH POLYADENYLATE RNA

Method: X-RAY DIFFRACTION D_max: 111.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cvj
deposition_date	1999-08-23
title	X-RAY CRYSTAL STRUCTURE OF THE POLY(A)-BINDING PROTEIN IN COMPLEX WITH POLYADENYLATE RNA
keywords	RRM, PROTEIN-RNA COMPLEX, GENE REGULATION-RNA COMPLEX; GENE REGULATION/RNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	36.19 Å
rg_electron	36.21 Å
i0	456353000.00
molecular_weight	158870.0 kDa
excluded_volume	192570 Å³
envelope_volume	261160 Å³
shell_volume	58512 Å³
envelope_diameter	121.7 Å
shell_rg	43.96 Å
envelope_rg	35.70 Å
shape_rg	36.24 Å
total_rg	36.60 Å
total_atoms	11109
n_residues	1304
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	111.2 Å
rg_real	35.98 Å
rg_real_error	0.66 Å
i0_real	4.5640e+08
i0_real_error	7.2180e+06
rg_reciprocal	36.11 Å
i0_reciprocal	456400000.0000
total_estimate	0.8904
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	48.0 Å
skewness	0.136
kurtosis	-0.372
angular_range	— – 0.2200 Å⁻¹
current_alpha	0.0000
highest_alpha	37280000.0000
n_real_points	45
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.907; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.979; Smooth: 0.871

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	5'-R(APAPAPAPAPAPAPAPAPAP*A)-3'
formula_weight	3576.3 kDa
src_method	syn
entity_count	8

Polymer

type	polyribonucleotide
strand_id	M,N,O,P,Q,R,S,T
atom_count	11162

AAAAAAAAAAA

Entity 2 polymer

description	POLYADENYLATE BINDING PROTEIN 1
formula_weight	21463.6 kDa
src_method	man
entity_count	8

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E,F,G,H

MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEF

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 3 non-polymer

description	ADENOSINE MONOPHOSPHATE
formula_weight	347.2 kDa
src_method	syn
entity_count	8

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	53

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6. Citations (1)

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length_a	79.557 Å
length_b	176.797 Å
length_c	82.489 Å
angle_alpha	90.00 °
angle_beta	117.67 °
angle_gamma	90.00 °
z_pdb	16

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	2.600 Å
resolution_low	20.000 Å
r_factor	0.2300
r_work	0.2300
r_free	0.3040

fit	/app/data/saxs_computation/cv/1cvj/1cvj.fit
pddf	/app/data/pr_computation/cv/1cvj/1cvj.pr.out
profile	/app/data/saxs_computation/cv/1cvj/1cvj.dat

X-RAY CRYSTAL STRUCTURE OF THE POLY(A)-BINDING PROTEIN IN COMPLEX WITH POLYADENYLATE RNA

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)