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1jh4

Solution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip1

Method: SOLUTION NMR Dmax: 46.4 Å Quality: REASONABLE
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1. Protein Identity and Related Structures Protein Identity & Related Structures

polyadenylate-binding protein 1

Homo sapiens

UniProt P11940

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 544–636 Fragment：C-terminal domain polyadenylate-binding protein-interacting protein-1 × 1 （Q9H074） SOLUTION NMR NMR measurement conditions：pH 6.3；303 K；Ionic strength (raw mmCIF value) 0.1M NaCl；Pressure ambient NMR sample composition：3mM 15N-labeled PABC; 3mM 15N-labeled peptide; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 90% H2O/10% D2O NMR sample composition：3mM unlabeled PABC; 3mM unlabeled peptide; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 90% H2O/10% D2O NMR sample composition：3mM unlabeled PABC; 3mM unlabeled peptide; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 100% D2O NMR sample composition：3mM unlabeled PABC; 3mM N15-labeled peptide; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 90% H2O/10% D2O Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1JH4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1CVJ X-RAY CRYSTAL STRUCTURE OF THE POLY(A)-BINDING PROTEIN IN COMPLEX WITH POLYADENYLATE RNA Deposited 1999-08-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric
Chain A 1–190（190 aa） Fragment：RESIDUES 1-190
Not recorded AMP ADENOSINE MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；296.7 K；AMMONIUM SULFATE, TCEP, TRIS-HCL, POTASSIUM CHLORIDE, GLYCEROL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.7K
Resolution 2.60 Å R-free 0.304
1CVJ X-RAY CRYSTAL STRUCTURE OF THE POLY(A)-BINDING PROTEIN IN COMPLEX WITH POLYADENYLATE RNA Deposited 1999-08-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric
Chain B 1–190（190 aa） Fragment：RESIDUES 1-190
Not recorded AMP ADENOSINE MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；296.7 K；AMMONIUM SULFATE, TCEP, TRIS-HCL, POTASSIUM CHLORIDE, GLYCEROL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.7K
Resolution 2.60 Å R-free 0.304
1CVJ X-RAY CRYSTAL STRUCTURE OF THE POLY(A)-BINDING PROTEIN IN COMPLEX WITH POLYADENYLATE RNA Deposited 1999-08-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric
Chain C 1–190（190 aa） Fragment：RESIDUES 1-190
Not recorded AMP ADENOSINE MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；296.7 K；AMMONIUM SULFATE, TCEP, TRIS-HCL, POTASSIUM CHLORIDE, GLYCEROL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.7K
Resolution 2.60 Å R-free 0.304
1CVJ X-RAY CRYSTAL STRUCTURE OF THE POLY(A)-BINDING PROTEIN IN COMPLEX WITH POLYADENYLATE RNA Deposited 1999-08-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric
Chain D 1–190（190 aa） Fragment：RESIDUES 1-190
Not recorded AMP ADENOSINE MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；296.7 K；AMMONIUM SULFATE, TCEP, TRIS-HCL, POTASSIUM CHLORIDE, GLYCEROL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.7K
Resolution 2.60 Å R-free 0.304
1CVJ X-RAY CRYSTAL STRUCTURE OF THE POLY(A)-BINDING PROTEIN IN COMPLEX WITH POLYADENYLATE RNA Deposited 1999-08-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric
Chain E 1–190（190 aa） Fragment：RESIDUES 1-190
Not recorded AMP ADENOSINE MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；296.7 K；AMMONIUM SULFATE, TCEP, TRIS-HCL, POTASSIUM CHLORIDE, GLYCEROL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.7K
Resolution 2.60 Å R-free 0.304
1CVJ X-RAY CRYSTAL STRUCTURE OF THE POLY(A)-BINDING PROTEIN IN COMPLEX WITH POLYADENYLATE RNA Deposited 1999-08-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric
Chain F 1–190（190 aa） Fragment：RESIDUES 1-190
Not recorded AMP ADENOSINE MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；296.7 K；AMMONIUM SULFATE, TCEP, TRIS-HCL, POTASSIUM CHLORIDE, GLYCEROL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.7K
Resolution 2.60 Å R-free 0.304
1CVJ X-RAY CRYSTAL STRUCTURE OF THE POLY(A)-BINDING PROTEIN IN COMPLEX WITH POLYADENYLATE RNA Deposited 1999-08-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric
Chain G 1–190（190 aa） Fragment：RESIDUES 1-190
Not recorded AMP ADENOSINE MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；296.7 K；AMMONIUM SULFATE, TCEP, TRIS-HCL, POTASSIUM CHLORIDE, GLYCEROL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.7K
Resolution 2.60 Å R-free 0.304
1CVJ X-RAY CRYSTAL STRUCTURE OF THE POLY(A)-BINDING PROTEIN IN COMPLEX WITH POLYADENYLATE RNA Deposited 1999-08-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 8 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric
Chain H 1–190（190 aa） Fragment：RESIDUES 1-190
Not recorded AMP ADENOSINE MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；296.7 K；AMMONIUM SULFATE, TCEP, TRIS-HCL, POTASSIUM CHLORIDE, GLYCEROL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.7K
Resolution 2.60 Å R-free 0.304
1G9L SOLUTION STRUCTURE OF THE PABC DOMAIN OF HUMAN POLY(A) BINDING PROTEIN Deposited 2000-11-24 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 498–636（139 aa） Fragment：C-TERMINAL DOMAIN (RESIDUES 498-636)
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.3；303 K；Ionic strength (raw mmCIF value) 0.2；Pressure ambient
NMR sample composition 3mM 15N-labeled PABC; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 90% H2O/10% D2O
NMR sample composition 3mM 15N,13C-labeled PABC; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 100% D2O
NMR sample composition 3mM unlabeled PABC; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 90% H2O/10% D2O
Resolution not provided
1JGN Solution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip2 Deposited 2001-06-26 Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 544–636（93 aa） Fragment：C-terminal domain
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.3；303 K；Ionic strength (raw mmCIF value) 0.1M NaCl；Pressure ambient
NMR sample composition 3mM 15N-labeled PABC; 4mM unlabeled peptide; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 90% H2O/10% D2O
NMR sample composition 3mM 15N,13C-labeled PABC; 4mM unlabeled peptide; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 100% D2O
NMR sample composition 2.5mM 15N-labeled peptide; 3mM unlabeled PABC; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 90% H2O/10% D2O
NMR sample composition 2mM 15N,13C-labeled peptide; 3mM unlabeled PABC; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 100% D2O
NMR sample composition 3mM unlabeled PABC; 3mM unlabeled peptide; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 90% H2O/10% D2O
NMR sample composition 3mM unlabeled PABC; 3mM unlabeled peptide; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 100% D2O
Resolution not provided
2K8G Solution structure of RRM2 domain of PABP1 Deposited 2008-09-09 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 90–182（93 aa） Fragment：RRM2 domain (UNP residues 90-182)
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.1；303 K；Ionic strength (raw mmCIF value) 0.04；Pressure ambient
NMR sample composition 1 mM [U-98% 13C; U-98% 15N] PABP1, 20 mM sodium chloride, 20 mM sodium phosphate, 1 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-98% 15N] PABP1, 20 mM sodium chloride, 20 mM sodium phosphate, 1 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM PABP1, 20 mM sodium chloride, 20 mM sodium phosphate, 1 mM DTT, 100% D2O | 100% D2O
Resolution not provided
2RQG Structure of GSPT1/ERF3A-PABC Deposited 2009-05-08 Different construct Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 541–623（83 aa） Fragment：PABC DOMAIN
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；303 K；Ionic strength (raw mmCIF value) 0.15M；Pressure AMBIENT
NMR sample composition 1 mM [U-98% 13C; U-98% 15N] G1 TO S PHASE TRANSITION 1-1, 1 mM [U-98% 13C; U-98% 15N] POLYADENYLATE-BINDING PROTEIN 1-2, 93% H2O/7% D2O | 93% H2O/7% D2O
Resolution not provided
2RQH Structure of GSPT1/ERF3A-PABC Deposited 2009-05-08 Different construct Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 541–623（83 aa） Fragment：PABC DOMAIN
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；303 K；Ionic strength (raw mmCIF value) 0.15M；Pressure AMBIENT
NMR sample composition 1 mM G1 TO S PHASE TRANSITION 1-1, 1 mM [U-98% 13C; U-98% 15N] POLYADENYLATE-BINDING PROTEIN 1-2, 93% H2O/7% D2O | 93% H2O/7% D2O
Resolution not provided
2X04 Crystal structure of the PABC-TNRC6C complex Deposited 2009-12-04 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 456–530（75 aa） Fragment：C-TERMINAL DOMAIN (PABC), RESIDUES 456-530
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；0.1 M NA-ACETATE PH 4.6, 200 MM AMMONIUM SULFATE, 30% (W/V) PEG 4000
Resolution 1.49 Å R-free 0.186
2X04 Crystal structure of the PABC-TNRC6C complex Deposited 2009-12-04 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 456–530（75 aa） Fragment：C-TERMINAL DOMAIN (PABC), RESIDUES 456-530
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；0.1 M NA-ACETATE PH 4.6, 200 MM AMMONIUM SULFATE, 30% (W/V) PEG 4000
Resolution 1.49 Å R-free 0.186
3KTP Structural basis of GW182 recognition by poly(A)-binding protein Deposited 2009-11-25 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 544–626（83 aa） Fragment：C-terminal domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；2.0 M ammonium sulfate, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.50 Å R-free 0.231
3KTR Structural basis of ataxin-2 recognition by poly(A)-binding protein Deposited 2009-11-25 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 544–626（83 aa） Fragment：C-terminal domain
Not recorded CD CADMIUM ION × 1 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；2.2 M ammonium sulfate, 0.2 M CdCl2, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.70 Å R-free 0.245
3KUI Crystal structure of C-terminal domain of PABPC1 in complex with binding region of eRF3a Deposited 2009-11-27 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 544–626（83 aa） Fragment：C-terminal domain
Not recorded ZN ZINC ION × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.4；295 K；2.1M ammonium sulfate, 0.2M sodium sulfate, 10mM zinc chloride, 0.1M sodium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.30 Å R-free 0.255
3KUJ Crystal structure of C-terminal domain of PABPC1 in complex with binding region of eRF3a Deposited 2009-11-27 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 544–626（83 aa） Fragment：C-terminal domain
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；295 K；1.4M ammonium sulfate, 0.1M citric acid, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.40 Å R-free 0.223
3KUR Crystal structure of the MLLE domain of poly(A)-binding protein Deposited 2009-11-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 544–617（74 aa） Fragment：C-terminal domain
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.3M ammonium sulfate, 0.5M lithium sulfate, 5% glycerol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.50 Å R-free 0.272
3KUR Crystal structure of the MLLE domain of poly(A)-binding protein Deposited 2009-11-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 544–617（74 aa） Fragment：C-terminal domain
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.3M ammonium sulfate, 0.5M lithium sulfate, 5% glycerol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.50 Å R-free 0.272
3KUR Crystal structure of the MLLE domain of poly(A)-binding protein Deposited 2009-11-27 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 544–617（74 aa） Fragment：C-terminal domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.3M ammonium sulfate, 0.5M lithium sulfate, 5% glycerol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.50 Å R-free 0.272
3KUR Crystal structure of the MLLE domain of poly(A)-binding protein Deposited 2009-11-27 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 544–617（74 aa） Fragment：C-terminal domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.3M ammonium sulfate, 0.5M lithium sulfate, 5% glycerol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.50 Å R-free 0.272
3KUR Crystal structure of the MLLE domain of poly(A)-binding protein Deposited 2009-11-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain E 544–617（74 aa） Fragment：C-terminal domain
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.3M ammonium sulfate, 0.5M lithium sulfate, 5% glycerol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.50 Å R-free 0.272
3KUR Crystal structure of the MLLE domain of poly(A)-binding protein Deposited 2009-11-27 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain F 544–617（74 aa） Fragment：C-terminal domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.3M ammonium sulfate, 0.5M lithium sulfate, 5% glycerol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.50 Å R-free 0.272
3KUR Crystal structure of the MLLE domain of poly(A)-binding protein Deposited 2009-11-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain G 544–617（74 aa） Fragment：C-terminal domain
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.3M ammonium sulfate, 0.5M lithium sulfate, 5% glycerol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.50 Å R-free 0.272
3KUR Crystal structure of the MLLE domain of poly(A)-binding protein Deposited 2009-11-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain H 544–617（74 aa） Fragment：C-terminal domain
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.3M ammonium sulfate, 0.5M lithium sulfate, 5% glycerol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.50 Å R-free 0.272
3KUS Crystal structure of the MLLE domain of poly(A)-binding protein in complex with the binding region of Paip2 Deposited 2009-11-27 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 544–626（83 aa） Fragment：C-terminal domain
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；2.3M ammonium sulfate, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.40 Å R-free 0.205
3KUS Crystal structure of the MLLE domain of poly(A)-binding protein in complex with the binding region of Paip2 Deposited 2009-11-27 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 544–626（83 aa） Fragment：C-terminal domain
Not recorded EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；2.3M ammonium sulfate, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.40 Å R-free 0.205
3KUT Crystal structure of the MLLE domain of poly(A)-binding protein in complex with the binding region of Paip2 Deposited 2009-11-27 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 544–626（83 aa） Fragment：C-terminal domain
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；2.25M ammonium sulfate, 0.2M KBr, 0.1 M Bis-Tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.50 Å R-free 0.201
3KUT Crystal structure of the MLLE domain of poly(A)-binding protein in complex with the binding region of Paip2 Deposited 2009-11-27 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 544–626（83 aa） Fragment：C-terminal domain
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；2.25M ammonium sulfate, 0.2M KBr, 0.1 M Bis-Tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.50 Å R-free 0.201
3PKN Crystal structure of MLLE domain of poly(A) binding protein in complex with PAM2 motif of La-related protein 4 (LARP4) Deposited 2010-11-11 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 544–626（83 aa） Fragment：MLLE domain (UNP residues 544-626)
Not recorded SO4 SULFATE ION × 1 IOD IODIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；0.25 M potassium iodide, 1.9 M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.80 Å R-free 0.269
3PKN Crystal structure of MLLE domain of poly(A) binding protein in complex with PAM2 motif of La-related protein 4 (LARP4) Deposited 2010-11-11 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 544–626（83 aa） Fragment：MLLE domain (UNP residues 544-626)
Not recorded SO4 SULFATE ION × 2 IOD IODIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；0.25 M potassium iodide, 1.9 M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.80 Å R-free 0.269
3PTH The PABC1 MLLE domain bound to the variant PAM2 motif of LARP4B Deposited 2010-12-03 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 543–621（79 aa） Fragment：UNP residues 543-621
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.5；289 K；2 ul protein solution at 43 mg/ml containing the 1.5 fold molar amount of peptide ligand was mixed with 2ul reservoir solution containing 1.5M magnesium sulfate, pH 6.5, VAPOR DIFFUSION, temperature 289K
Resolution 1.70 Å R-free 0.218
4F02 Crystal structure of the PABP-binding site of eIF4G in complex with RRM1-2 of PABP and poly(A) Deposited 2012-05-03 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 2 PDB declaration: trimeric
Chain A 1–190（190 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；294 K；0.03 M Tris, 1.3 M (NH4)2SO4, 5% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 2.00 Å R-free 0.230
4F02 Crystal structure of the PABP-binding site of eIF4G in complex with RRM1-2 of PABP and poly(A) Deposited 2012-05-03 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 2 PDB declaration: trimeric
Chain D 1–190（190 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；294 K；0.03 M Tris, 1.3 M (NH4)2SO4, 5% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 2.00 Å R-free 0.230
4F25 Crystal structure of the second RRM domain of human PABPC1 at pH 6.0 Deposited 2012-05-07 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 99–199（101 aa） Fragment：RRM2 domain (un residues 99-119)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；294 K；25% PEG 1500 and 0.1 M MIB buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 1.90 Å R-free 0.269
4F26 Crystal structure of the second RRM domain of human PABPC1 a pH 9.0 Deposited 2012-05-07 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 99–199（101 aa） Fragment：RRM2 domain (un residues 99-119)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；294 K；25% PEG 1500 and 0.1 M MMT buffer , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 2.00 Å R-free 0.286
5DX1 Crystal structure of CARM1, sinefungin, and PABP1 peptide (R455) Deposited 2015-09-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain F 449–466（18 aa） Fragment：UNP residues 449-466
Chain G 449–466（18 aa） Fragment：UNP residues 449-466
Mutation：Nterminal biotin and aminohexanoic acid, methylated R460 Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：Nterminal biotin and aminohexanoic acid, methylated R460 Non-standard monomer：Yes (specific site not provided by mmCIF) SFG SINEFUNGIN × 2 GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；0.2 M Ammonium Sulfate, 0.1 M Tris pH 8.5, 18% w/v PEG 3350
Resolution 1.93 Å R-free 0.238
5DX1 Crystal structure of CARM1, sinefungin, and PABP1 peptide (R455) Deposited 2015-09-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain H 449–466（18 aa） Fragment：UNP residues 449-466
Chain I 449–466（18 aa） Fragment：UNP residues 449-466
Mutation：Nterminal biotin and aminohexanoic acid, methylated R460 Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：Nterminal biotin and aminohexanoic acid, methylated R460 Non-standard monomer：Yes (specific site not provided by mmCIF) SFG SINEFUNGIN × 2 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；0.2 M Ammonium Sulfate, 0.1 M Tris pH 8.5, 18% w/v PEG 3350
Resolution 1.93 Å R-free 0.238
5DX8 Crystal structure of CARM1, sinefungin, and methylated PABP1 peptide (R455) Deposited 2015-09-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain E 449–466（18 aa） Fragment：UNP residues 449-466
Chain F 449–466（18 aa） Fragment：UNP residues 449-466
Mutation：Nterminal biotin and aminohexanoic acid, methylated R455 and R460 Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：Nterminal biotin and aminohexanoic acid, methylated R455 and R460 Non-standard monomer：Yes (specific site not provided by mmCIF) SFG SINEFUNGIN × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；0.2 M Ammonium Sulfate, 0.1 M Tris pH 8.5, 18% w/v PEG 3350
Resolution 1.94 Å R-free 0.246
5DX8 Crystal structure of CARM1, sinefungin, and methylated PABP1 peptide (R455) Deposited 2015-09-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain G 449–466（18 aa） Fragment：UNP residues 449-466
Chain H 449–466（18 aa） Fragment：UNP residues 449-466
Mutation：Nterminal biotin and aminohexanoic acid, methylated R455 and R460 Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：Nterminal biotin and aminohexanoic acid, methylated R455 and R460 Non-standard monomer：Yes (specific site not provided by mmCIF) SFG SINEFUNGIN × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；0.2 M Ammonium Sulfate, 0.1 M Tris pH 8.5, 18% w/v PEG 3350
Resolution 1.94 Å R-free 0.246
5DXA Crystal structure of CARM1, sinefungin, and methylated PABP1 peptide (R460) Deposited 2015-09-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain F 449–466（18 aa） Fragment：UNP residues 449-466
Chain G 449–466（18 aa） Fragment：UNP residues 449-466
Mutation：Nterminal biotin and aminohexanoic acid, methylated R460, asymmetrically dimethylated R455 Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：Nterminal biotin and aminohexanoic acid, methylated R460, asymmetrically dimethylated R455 Non-standard monomer：Yes (specific site not provided by mmCIF) SFG SINEFUNGIN × 2 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；0.2 M Ammonium Sulfate, 0.1 M Tris pH 8.5, 18% w/v PEG 3350
Resolution 2.07 Å R-free 0.244
5DXA Crystal structure of CARM1, sinefungin, and methylated PABP1 peptide (R460) Deposited 2015-09-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain I 449–466（18 aa） Fragment：UNP residues 449-466
Mutation：Nterminal biotin and aminohexanoic acid, methylated R460, asymmetrically dimethylated R455 Non-standard monomer：Yes (specific site not provided by mmCIF) SFG SINEFUNGIN × 2 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；0.2 M Ammonium Sulfate, 0.1 M Tris pH 8.5, 18% w/v PEG 3350
Resolution 2.07 Å R-free 0.244
5LGQ Crystal structure of mouse CARM1 in complex with ligand P2C3s Deposited 2016-07-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 456–466（11 aa）
Chain F 456–466（11 aa）
Chain G 456–466（11 aa）
Chain H 456–466（11 aa）
Not recorded EDO 1,2-ETHANEDIOL × 7 PEG DI(HYDROXYETHYL)ETHER × 4 DXE 1,2-DIMETHOXYETHANE × 1 PG6 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE × 1 SO4 SULFATE ION × 1 8ZB (2~{R},3~{R},4~{S},5~{R})-2-(6-aminopurin-9-yl)-5-propyl-oxolane-3,4-diol × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；100 mM Tris-HCl pH 8.5 14% PEG 3350 200 mM A.S.
Resolution 2.11 Å R-free 0.236
5LGR Crystal structure of mouse CARM1 in complex with ligand P1C3u Deposited 2016-07-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 447–458（12 aa）
Chain F 447–458（12 aa）
Chain G 447–458（12 aa）
Chain H 447–458（12 aa）
Not recorded SO4 SULFATE ION × 1 EDO 1,2-ETHANEDIOL × 6 DXE 1,2-DIMETHOXYETHANE × 1 PG6 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE × 1 PEG DI(HYDROXYETHYL)ETHER × 1 QVR (2~{R},3~{R},4~{S},5~{R})-2-(6-aminopurin-9-yl)-5-[(~{E})-prop-1-enyl]oxolane-3,4-diol × 4 LPD L-PROLINAMIDE × 4 ACE ACETYL GROUP × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；Tris-HCl pH 8.5 100 mM PEG 3350 14 % Ammonium Sulfate 200 mM
Resolution 2.00 Å R-free 0.234
5LGS Crystal structure of mouse CARM1 in complex with ligand P2C3u Deposited 2016-07-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 456–464（9 aa）
Chain F 456–464（9 aa）
Chain G 456–464（9 aa）
Chain H 456–464（9 aa）
Not recorded SO4 SULFATE ION × 1 DXE 1,2-DIMETHOXYETHANE × 3 PEG DI(HYDROXYETHYL)ETHER × 1 PG4 TETRAETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 3 QVR (2~{R},3~{R},4~{S},5~{R})-2-(6-aminopurin-9-yl)-5-[(~{E})-prop-1-enyl]oxolane-3,4-diol × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；Tris-HCl pH 8.5 100 mM PEG 3350 20 % A.S. 200 mM
Resolution 2.10 Å R-free 0.215
7BN3 Crystal structure of C-terminal domain of PABPC1 in complex with Nucleoprotein from Human Coronavirus 229E Deposited 2021-01-21 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 455–537（83 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；0.1 M MES sodium salt pH-6.5, 1.8 M Ammonium Sulfate
Resolution 1.93 Å R-free 0.228
7BN3 Crystal structure of C-terminal domain of PABPC1 in complex with Nucleoprotein from Human Coronavirus 229E Deposited 2021-01-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 455–537（83 aa）
Not recorded SO4 SULFATE ION × 2 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；0.1 M MES sodium salt pH-6.5, 1.8 M Ammonium Sulfate
Resolution 1.93 Å R-free 0.228
7BN3 Crystal structure of C-terminal domain of PABPC1 in complex with Nucleoprotein from Human Coronavirus 229E Deposited 2021-01-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 455–537（83 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；0.1 M MES sodium salt pH-6.5, 1.8 M Ammonium Sulfate
Resolution 1.93 Å R-free 0.228
8SMO Crystal structure of the complex between truncated MLLE domain of PABPC1 and engineered superPAM2 peptide Deposited 2023-04-26 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 556–626（71 aa） Fragment：MLLE domain
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；0.1 M citric acid pH 4.0, 1.6 M ammonium sulfate
Resolution 3.00 Å R-free 0.292
8SMO Crystal structure of the complex between truncated MLLE domain of PABPC1 and engineered superPAM2 peptide Deposited 2023-04-26 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 556–626（71 aa） Fragment：MLLE domain
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；0.1 M citric acid pH 4.0, 1.6 M ammonium sulfate
Resolution 3.00 Å R-free 0.292
8SMO Crystal structure of the complex between truncated MLLE domain of PABPC1 and engineered superPAM2 peptide Deposited 2023-04-26 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 556–626（71 aa） Fragment：MLLE domain
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；0.1 M citric acid pH 4.0, 1.6 M ammonium sulfate
Resolution 3.00 Å R-free 0.292
8SMO Crystal structure of the complex between truncated MLLE domain of PABPC1 and engineered superPAM2 peptide Deposited 2023-04-26 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 556–626（71 aa） Fragment：MLLE domain
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；0.1 M citric acid pH 4.0, 1.6 M ammonium sulfate
Resolution 3.00 Å R-free 0.292
8SMO Crystal structure of the complex between truncated MLLE domain of PABPC1 and engineered superPAM2 peptide Deposited 2023-04-26 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain I 556–626（71 aa） Fragment：MLLE domain
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；0.1 M citric acid pH 4.0, 1.6 M ammonium sulfate
Resolution 3.00 Å R-free 0.292
8SMO Crystal structure of the complex between truncated MLLE domain of PABPC1 and engineered superPAM2 peptide Deposited 2023-04-26 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain K 556–626（71 aa） Fragment：MLLE domain
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；0.1 M citric acid pH 4.0, 1.6 M ammonium sulfate
Resolution 3.00 Å R-free 0.292
8SMO Crystal structure of the complex between truncated MLLE domain of PABPC1 and engineered superPAM2 peptide Deposited 2023-04-26 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain M 556–626（71 aa） Fragment：MLLE domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；0.1 M citric acid pH 4.0, 1.6 M ammonium sulfate
Resolution 3.00 Å R-free 0.292
8SMO Crystal structure of the complex between truncated MLLE domain of PABPC1 and engineered superPAM2 peptide Deposited 2023-04-26 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain O 556–626（71 aa） Fragment：MLLE domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；0.1 M citric acid pH 4.0, 1.6 M ammonium sulfate
Resolution 3.00 Å R-free 0.292

27 other PDB entries and 58 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PABP1_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 6–98； UniProt 544–636

polyadenylate-binding protein-interacting protein-1

Homo sapiens

UniProt Q9H074

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain B； UniProt 124–145 Fragment：22-residue fragment polyadenylate-binding protein 1 × 1 （P11940） SOLUTION NMR NMR measurement conditions：pH 6.3；303 K；Ionic strength (raw mmCIF value) 0.1M NaCl；Pressure ambient NMR sample composition：3mM 15N-labeled PABC; 3mM 15N-labeled peptide; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 90% H2O/10% D2O NMR sample composition：3mM unlabeled PABC; 3mM unlabeled peptide; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 90% H2O/10% D2O NMR sample composition：3mM unlabeled PABC; 3mM unlabeled peptide; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 100% D2O NMR sample composition：3mM unlabeled PABC; 3mM N15-labeled peptide; 50mM phosphate buffer; 0.1M NaCl; 1mM NaN3; pH 6.3 | 90% H2O/10% D2O Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1JH4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3NTW Structure of the MLLE domain of EDD in complex with a PAM2 peptide from Paip1 Deposited 2010-07-05 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 123–144（22 aa） Fragment：UNP residues 123-144, PAM2 motif
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；1.0 M ammonium sulfate, 0.2 M lithium sulfate, 10% glycerol and 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.60 Å R-free 0.290
3NTW Structure of the MLLE domain of EDD in complex with a PAM2 peptide from Paip1 Deposited 2010-07-05 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 123–144（22 aa） Fragment：UNP residues 123-144, PAM2 motif
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；1.0 M ammonium sulfate, 0.2 M lithium sulfate, 10% glycerol and 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.60 Å R-free 0.290
3RK6 Crystal structure of the middle domain of human Paip1 Deposited 2011-04-17 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 157–373（217 aa） Fragment：middle domain (MIF4G, UNP residues 157-373)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；18% PEG20000, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.00 Å R-free 0.244
3RK6 Crystal structure of the middle domain of human Paip1 Deposited 2011-04-17 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 157–373（217 aa） Fragment：middle domain (MIF4G, UNP residues 157-373)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；18% PEG20000, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.00 Å R-free 0.244
6YXJ Crystal structure of SARS-CoV macrodomain II in complex with human Paip1 Deposited 2020-05-02 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 157–375（219 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.1 M (NH4)2SO4, 0.1 M HEPES pH 7.3, 18% PEG 3,350, 15% glycerol
Resolution 3.50 Å R-free 0.336

3 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PAIP1_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–22； UniProt 124–145

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1jh4

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1jh4
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1jh4
Deposition date deposition_date2001-06-27
Structure title titleSolution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip1
Keywords keywordsall-helical domain, protein-peptide complex, RNA BINDING PROTEIN; RNA BINDING PROTEIN
Experimental Method methodSOLUTION NMR
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier17.21 Å
Radius of gyration Rg (electron density) rg_electron17.26 Å
Forward intensity I(0) i01994230000.00
Molecular weight molecular_weight382850.0 kDa
Excluded volume excluded_volume481520 ų
Envelope volume envelope_volume87652 ų
Hydration-shell volume shell_volume27671 ų
Envelope diameter envelope_diameter85.8 Å
Shell Rg shell_rg33.46 Å
Envelope Rg envelope_rg28.84 Å
Shape Rg shape_rg17.25 Å
Total Rg total_rg17.65 Å
Total atoms total_atoms54120
Residues n_residues3600
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax46.4 Å
Rg (real space) rg_real15.86 Å
Rg uncertainty (real space) rg_real_error0.10 Å
I(0) (real space) i0_real1.8950e+09
I(0) uncertainty (real space) i0_real_error1.6770e+07
Rg (reciprocal space) rg_reciprocal17.50 Å
I(0) (reciprocal space) i0_reciprocal1994000000.0000
Solution quality estimate total_estimate0.6814
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary17.1 Å
Skewness Skewness skewness0.399
Kurtosis Kurtosis kurtosis-0.356
Angular range angular_range— – 0.4600 Å−1
Current regularization parameter α current_alpha2.8600
Highest regularization parameter α highest_alpha266800.0000
Real-space data points n_real_points77
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.009; Oscil: 0.972; Stabil: 0.986; Sysdev: 0.000; Positv: 1.000; Valcen: 0.987; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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7. Fold Classification (SCOP + CATH) 3 domains

SCOP 2.08 (2 domains)

Domain ID domain_idd1jh4a1
Class classa — All alpha proteins
Fold Fold folda.144 — PABP domain-like
Superfamily Superfamily superfamilya.144.1 — PABC (PABP) domain
Family Family familya.144.1.1 — PABC (PABP) domain
Domain ID domain_idd1jh4a2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags

CATH v4.4 (1 domains)

Domain ID domain_id1jh4A01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology1900 — c-terminal domain of poly(a) binding protein
Homologous superfamily homologous superfamily10 — c-terminal domain of poly(a) binding protein
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8. Citations (2)

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9. Files and Curves (10)