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1cws

HUMAN CDC25B CATALYTIC DOMAIN WITH TUNGSTATE

Method: X-RAY DIFFRACTION D_max: 59.0 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cws
deposition_date	1999-08-26
title	HUMAN CDC25B CATALYTIC DOMAIN WITH TUNGSTATE
keywords	HYDROLASE, CELL CYCLE PHOSPHATASE, DUAL SPECIFICITY PROTEIN PHOSPHATASE, CDC25, CDC25B, CELL CYCLE; CELL CYCLE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	17.48 Å
rg_electron	16.39 Å
i0	8741740.00
molecular_weight	21472.0 kDa
excluded_volume	26654 Å³
envelope_volume	30486 Å³
shell_volume	15665 Å³
envelope_diameter	57.7 Å
shell_rg	22.47 Å
envelope_rg	16.79 Å
shape_rg	16.43 Å
total_rg	17.28 Å
total_atoms	1497
n_residues	179
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	59.0 Å
rg_real	17.41 Å
rg_real_error	0.41 Å
i0_real	8.7420e+06
i0_real_error	1.0350e+05
rg_reciprocal	17.42 Å
i0_reciprocal	8742000.0000
total_estimate	0.7979
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	22.0 Å
skewness	0.240
kurtosis	-0.265
angular_range	— – 0.4550 Å⁻¹
current_alpha	0.0000
highest_alpha	1651000.0000
n_real_points	76
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.792; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.992; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (6)

Entity 1 polymer

description	CDC25 B-TYPE TYROSINE PHOSPHATASE
formula_weight	24907.5 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	1666

VLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 2 non-polymer

description	TUNGSTATE(VI)ION
formula_weight	247.8 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	1

Entity 4 non-polymer

description	CHLORIDE ION
formula_weight	35.5 kDa
src_method	syn
entity_count	1

Entity 5 non-polymer

description	BETA-MERCAPTOETHANOL
formula_weight	78.1 kDa
src_method	syn
entity_count	1

Entity 6 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	163

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6. Citations (1)

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length_a	51.734 Å
length_b	71.863 Å
length_c	74.935 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	2.000 Å
resolution_low	10.000 Å
r_factor	0.1680
r_work	—
r_free	—

fit	/app/data/saxs_computation/cw/1cws/1cws.fit
pddf	/app/data/pr_computation/cw/1cws/1cws.pr.out
profile	/app/data/saxs_computation/cw/1cws/1cws.dat